P23371 (PAP1_VACCW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly(A) polymerase catalytic subunit
EC=2.7.7.19

Alternative name(s):
Poly(A) polymerase large subunit
Short name=PAP-L
VP55

Gene names

Name:	PAPL
Ordered Locus Names:	VACWR057
ORF Names:	E1L

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)) [Reference proteome]

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's. Ref.3
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.
Sequence similarities	Belongs to the poxviridae poly(A) polymerase catalytic subunit family.

Ontologies

Keywords
Biological process	Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 479

479

Poly(A) polymerase catalytic subunit

PRO_0000099107

Sites

Active site

202

1

By similarity

Active site

204

1

By similarity

Experimental info

Sequence conflict

10

1

L → F in AAB59821. Ref.1

Sequence conflict

68

1

N → D in AAB59821. Ref.1

Sequence conflict

199

1

R → L in AAB59821. Ref.1

Secondary structure

1

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479

Helix Strand Turn

Details...

Helix

13 – 22

10

Helix

28 – 34

7

Helix

35 – 37

3

Helix

38 – 46

9

Helix

49 – 63

15

Helix

71 – 81

11

Helix

82 – 87

6

Helix

91 – 111

21

Helix

113 – 116

4

Helix

131 – 134

4

Helix

135 – 145

11

Helix

165 – 178

14

Turn

180 – 182

3

Beta strand

183 – 187

5

Helix

188 – 192

5

Beta strand

204 – 209

6

Helix

210 – 225

16

Beta strand

230 – 233

4

Beta strand

237 – 244

8

Beta strand

250 – 256

7

Helix

259 – 264

6

Beta strand

267 – 270

4

Beta strand

273 – 276

4

Helix

278 – 288

11

Helix

292 – 300

9

Helix

302 – 320

19

Beta strand

335 – 338

4

Turn

339 – 342

4

Beta strand

343 – 347

5

Turn

349 – 351

3

Beta strand

352 – 361

10

Helix

363 – 372

10

Helix

382 – 384

3

Beta strand

386 – 392

7

Beta strand

395 – 399

5

Beta strand

405 – 408

4

Helix

414 – 430

17

Helix

436 – 444

9

Beta strand

468 – 471

4

Turn

472 – 475

4

Beta strand

476 – 478

3

Sequences

	Sequence		Length	Mass (Da)
	P23371 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: 2E76F2BB1A246D5F	FASTA	479	55,531
	10 20 30 40 50 60 MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD IFVSLVKKNK 70 80 90 100 110 120 KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTD ILGVLTIKAP 130 140 150 160 170 180 NVISSKISYN VTSMEELARD MLNSMNVAVI DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN 190 200 210 220 230 240 KSCICYGSYS LYLINPNIRY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY 250 260 270 280 290 300 MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK 310 320 330 340 350 360 DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD YFSFKKCLVY 370 380 390 400 410 420 LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL 430 440 450 460 470 CAHILLYQML TSGEYKQCLS DLLNSMMNRD KIPIYSHTER DKKPGRHGFI NIEKDIIVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Gershon P.D., Jones E.V., Moss B., Ahn B.-Y. Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus." Gershon P.D., Ahn B.-Y., Garfield M., Moss B. Cell 66:1269-1278(1991) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M36339 Genomic DNA. Translation: AAB59821.1.
AY243312 Genomic DNA. Translation: AAO89336.1.

RefSeq

YP_232939.1. NC_006998.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GA9	X-ray	2.30	D	11-479	[»]
2GAF	X-ray	2.40	D	11-479	[»]
3ER8	X-ray	3.18	C/D	1-479	[»]
3ER9	X-ray	2.06	B	1-479	[»]
3ERC	X-ray	3.21	C/D	1-479	[»]
3OWG	X-ray	2.86	A/B	1-479	[»]

ProteinModelPortal

P23371.

SMR

P23371. Positions 12-479.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48309N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3707514.

Phylogenomic databases

ProtClustDB

PHA2996.

Family and domain databases

InterPro

IPR024231. Pox_polyA_pol_nucTrfase.
IPR004976. Poxvirus_polyA_pol_cat.
IPR024397. Poxvirus_polyA_pol_cat_C.
IPR024398. Poxvirus_polyA_pol_cat_N.
[Graphical view]

Pfam

PF03296. Pox_polyA_pol. 1 hit.
PF12629. Pox_polyA_pol_C. 1 hit.
PF12630. Pox_polyA_pol_N. 1 hit.
[Graphical view]

PIRSF

PIRSF015693. VAC-48L_nuct. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P23371.

Entry information

Entry name

PAP1_VACCW

Accession

Primary (citable) accession number: P23371
Secondary accession number(s): Q80HX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	December 7, 2004
Last modified:	April 3, 2013
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program