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Reviewed, UniProtKB/Swiss-Prot P23371 (PAP1_VACCW)

Last modified June 16, 2009. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Poly(A) polymerase catalytic subunit
    EC=2.7.7.19
Alternative name(s):
    Poly(A) polymerase large subunit
      Short name=PAP-L
    VP55
Gene names
Name: PAPL
Ordered Locus Names: VACWR057
ORF Names: E1L
OrganismVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifier10254 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirus
Virus hostBos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Polymerase that creates the 3'-poly(A) tail of mRNA's. Ref.3

Catalytic activity

ATP + RNA(n) = diphosphate + RNA(n+1).

Subunit structure

Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.

Sequence similarities

Belongs to the poxviridae poly(A) polymerase catalytic subunit family.

Ontologies

Keywords
   Biological processTranscription
mRNA processing
   LigandATP-binding
Nucleotide-binding
   Molecular functionTransferase
   Technical term3D-structure
Gene Ontology (GO)
   Biological processmRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

transcription

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

polynucleotide adenylyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 479479Poly(A) polymerase catalytic subunit
PRO_0000099107

Sites

Active site2021 By similarity
Active site2041 By similarity

Experimental info

Sequence conflict101L → F in AAB59821. Ref.1
Sequence conflict681N → D in AAB59821. Ref.1
Sequence conflict1991R → L in AAB59821. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P23371-1 [UniParc].

Last modified December 7, 2004. Version 2.
Checksum: 2E76F2BB1A246D5F

FASTA47955,531
        10         20         30         40         50         60 
MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD IFVSLVKKNK 

        70         80         90        100        110        120 
KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTD ILGVLTIKAP 

       130        140        150        160        170        180 
NVISSKISYN VTSMEELARD MLNSMNVAVI DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN 

       190        200        210        220        230        240 
KSCICYGSYS LYLINPNIRY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY 

       250        260        270        280        290        300 
MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK 

       310        320        330        340        350        360 
DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD YFSFKKCLVY 

       370        380        390        400        410        420 
LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL 

       430        440        450        460        470 
CAHILLYQML TSGEYKQCLS DLLNSMMNRD KIPIYSHTER DKKPGRHGFI NIEKDIIVF 

« Hide

References

« Hide 'large scale' references
[1]Gershon P.D., Jones E.V., Moss B., Ahn B.-Y.
Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus."
Gershon P.D., Ahn B.-Y., Garfield M., Moss B.
Cell 66:1269-1278(1991) [PubMed: 1670500] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

M36339 Genomic DNA. Translation: AAB59821.1.
AY243312 Genomic DNA. Translation: AAO89336.1.
RefSeqYP_232939.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2GA9X-ray2.30D11-479[»]
2GAFX-ray2.40D11-479[»]
SMRP23371. Positions 12-479.
ModBaseSearch...

Genome annotation databases

GeneID3707514.

Family and domain databases

InterProIPR004976. PolyA_pol_cat_poxviral.
[Graphical view]
PfamPF03296. Pox_polyA_pol. 1 hit.
[Graphical view]
PIRSFPIRSF015693. VAC-48L_nuct. 1 hit.
ProtoNetSearch...

Entry information

Entry namePAP1_VACCW
AccessionPrimary (citable) accession number: P23371
Secondary accession number(s): Q80HX2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: December 7, 2004
Last modified: June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectVirus (Virus annotation project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents