Reviewed, UniProtKB/Swiss-Prot P23371 (PAP1_VACCW)

Names and origin

Protein names

Recommended name:
    Poly(A) polymerase catalytic subunit
    EC=2.7.7.19
Alternative name(s):
    Poly(A) polymerase large subunit
      Short name=PAP-L
    VP55

Gene names

Name:	PAPL
Ordered Locus Names:	VACWR057
ORF Names:	E1L

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	479 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Polymerase that creates the 3'-poly(A) tail of mRNA's. Ref.3
Catalytic activity	ATP + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.
Sequence similarities	Belongs to the poxviridae poly(A) polymerase catalytic subunit family.

Ontologies

Keywords
Biological process	Transcription mRNA processing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polynucleotide adenylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 479	479	Poly(A) polymerase catalytic subunit		PRO_0000099107
Sites
	Active site	202	1	By similarity
	Active site	204	1	By similarity
Experimental info
	Sequence conflict	10	1	L → F in AAB59821. Ref.1
	Sequence conflict	68	1	N → D in AAB59821. Ref.1
	Sequence conflict	199	1	R → L in AAB59821. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23371-1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: 2E76F2BB1A246D5F	FASTA	479	55,531
	10 20 30 40 50 60 MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD IFVSLVKKNK 70 80 90 100 110 120 KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL QSVLVTTYTD ILGVLTIKAP 130 140 150 160 170 180 NVISSKISYN VTSMEELARD MLNSMNVAVI DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN 190 200 210 220 230 240 KSCICYGSYS LYLINPNIRY GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY 250 260 270 280 290 300 MVIKDENDNH IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK 310 320 330 340 350 360 DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD YFSFKKCLVY 370 380 390 400 410 420 LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF SNTILLSDKG KVHEISARGL 430 440 450 460 470 CAHILLYQML TSGEYKQCLS DLLNSMMNRD KIPIYSHTER DKKPGRHGFI NIEKDIIVF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Gershon P.D., Jones E.V., Moss B., Ahn B.-Y. Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus." Gershon P.D., Ahn B.-Y., Garfield M., Moss B. Cell 66:1269-1278(1991) [PubMed: 1670500] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M36339 Genomic DNA. Translation: AAB59821.1.
AY243312 Genomic DNA. Translation: AAO89336.1.

RefSeq

YP_232939.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2GA9	X-ray	2.30	D	11-479	[»]
2GAF	X-ray	2.40	D	11-479	[»]

SMR

P23371. Positions 12-479.

ModBase

Search...

Genome annotation databases

GeneID

3707514.

Family and domain databases

InterPro

IPR004976. PolyA_pol_cat_poxviral.
[Graphical view]

Pfam

PF03296. Pox_polyA_pol. 1 hit.
[Graphical view]

PIRSF

PIRSF015693. VAC-48L_nuct. 1 hit.

ProtoNet

Search...

Entry information

Entry name

PAP1_VACCW

Accession

Primary (citable) accession number: P23371
Secondary accession number(s): Q80HX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)