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P23371

- PAP1_VACCW

UniProt

P23371 - PAP1_VACCW

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Protein

Poly(A) polymerase catalytic subunit

Gene

PAPL

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei202 – 2021By similarity
Active sitei204 – 2041By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. mRNA processing Source: UniProtKB-KW
  2. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing, Transcription

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase catalytic subunit (EC:2.7.7.19)
Alternative name(s):
Poly(A) polymerase large subunit
Short name:
PAP-L
VP55
Gene namesi
Name:PAPL
Ordered Locus Names:VACWR057
ORF Names:E1L
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000000344: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 479479Poly(A) polymerase catalytic subunitPRO_0000099107Add
BLAST

Interactioni

Subunit structurei

Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.

Protein-protein interaction databases

DIPiDIP-48309N.

Structurei

Secondary structure

1
479
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi13 – 2210Combined sources
Helixi28 – 347Combined sources
Helixi35 – 373Combined sources
Helixi38 – 469Combined sources
Helixi49 – 6315Combined sources
Helixi71 – 8111Combined sources
Helixi82 – 876Combined sources
Helixi91 – 11121Combined sources
Helixi113 – 1164Combined sources
Helixi131 – 1344Combined sources
Helixi135 – 14511Combined sources
Helixi165 – 17814Combined sources
Turni180 – 1823Combined sources
Beta strandi183 – 1875Combined sources
Helixi188 – 1925Combined sources
Beta strandi204 – 2096Combined sources
Helixi210 – 22516Combined sources
Beta strandi230 – 2334Combined sources
Beta strandi237 – 2448Combined sources
Beta strandi250 – 2567Combined sources
Helixi259 – 2646Combined sources
Beta strandi267 – 2704Combined sources
Beta strandi273 – 2764Combined sources
Helixi278 – 28811Combined sources
Helixi292 – 3009Combined sources
Helixi302 – 32019Combined sources
Beta strandi335 – 3384Combined sources
Turni339 – 3424Combined sources
Beta strandi343 – 3475Combined sources
Turni349 – 3513Combined sources
Beta strandi352 – 36110Combined sources
Helixi363 – 37210Combined sources
Helixi382 – 3843Combined sources
Beta strandi386 – 3927Combined sources
Beta strandi395 – 3995Combined sources
Beta strandi405 – 4084Combined sources
Helixi414 – 43017Combined sources
Helixi436 – 4449Combined sources
Beta strandi468 – 4714Combined sources
Turni472 – 4754Combined sources
Beta strandi476 – 4783Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GA9X-ray2.30D11-479[»]
2GAFX-ray2.40D11-479[»]
3ER8X-ray3.18C/D1-479[»]
3ER9X-ray2.06B1-479[»]
3ERCX-ray3.21C/D1-479[»]
3OWGX-ray2.86A/B1-479[»]
ProteinModelPortaliP23371.
SMRiP23371. Positions 12-479.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23371.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR024231. Pox_polyA_pol_nucTrfase.
IPR004976. Poxvirus_polyA_pol_cat.
IPR024397. Poxvirus_polyA_pol_cat_C.
IPR024398. Poxvirus_polyA_pol_cat_N.
[Graphical view]
PfamiPF03296. Pox_polyA_pol. 1 hit.
PF12629. Pox_polyA_pol_C. 1 hit.
PF12630. Pox_polyA_pol_N. 1 hit.
[Graphical view]
PIRSFiPIRSF015693. VAC-48L_nuct. 1 hit.

Sequencei

Sequence statusi: Complete.

P23371-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD
60 70 80 90 100
IFVSLVKKNK KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL
110 120 130 140 150
QSVLVTTYTD ILGVLTIKAP NVISSKISYN VTSMEELARD MLNSMNVAVI
160 170 180 190 200
DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN KSCICYGSYS LYLINPNIRY
210 220 230 240 250
GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY MVIKDENDNH
260 270 280 290 300
IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK
310 320 330 340 350
DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD
360 370 380 390 400
YFSFKKCLVY LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF
410 420 430 440 450
SNTILLSDKG KVHEISARGL CAHILLYQML TSGEYKQCLS DLLNSMMNRD
460 470
KIPIYSHTER DKKPGRHGFI NIEKDIIVF
Length:479
Mass (Da):55,531
Last modified:December 7, 2004 - v2
Checksum:i2E76F2BB1A246D5F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101L → F in AAB59821. 1 PublicationCurated
Sequence conflicti68 – 681N → D in AAB59821. 1 PublicationCurated
Sequence conflicti199 – 1991R → L in AAB59821. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36339 Genomic DNA. Translation: AAB59821.1.
AY243312 Genomic DNA. Translation: AAO89336.1.
RefSeqiYP_232939.1. NC_006998.1.

Genome annotation databases

GeneIDi3707514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36339 Genomic DNA. Translation: AAB59821.1 .
AY243312 Genomic DNA. Translation: AAO89336.1 .
RefSeqi YP_232939.1. NC_006998.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2GA9 X-ray 2.30 D 11-479 [» ]
2GAF X-ray 2.40 D 11-479 [» ]
3ER8 X-ray 3.18 C/D 1-479 [» ]
3ER9 X-ray 2.06 B 1-479 [» ]
3ERC X-ray 3.21 C/D 1-479 [» ]
3OWG X-ray 2.86 A/B 1-479 [» ]
ProteinModelPortali P23371.
SMRi P23371. Positions 12-479.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48309N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3707514.

Miscellaneous databases

EvolutionaryTracei P23371.

Family and domain databases

InterProi IPR024231. Pox_polyA_pol_nucTrfase.
IPR004976. Poxvirus_polyA_pol_cat.
IPR024397. Poxvirus_polyA_pol_cat_C.
IPR024398. Poxvirus_polyA_pol_cat_N.
[Graphical view ]
Pfami PF03296. Pox_polyA_pol. 1 hit.
PF12629. Pox_polyA_pol_C. 1 hit.
PF12630. Pox_polyA_pol_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF015693. VAC-48L_nuct. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Gershon P.D., Jones E.V., Moss B., Ahn B.-Y.
    Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
    Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus."
    Gershon P.D., Ahn B.-Y., Garfield M., Moss B.
    Cell 66:1269-1278(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPAP1_VACCW
AccessioniPrimary (citable) accession number: P23371
Secondary accession number(s): Q80HX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: December 7, 2004
Last modified: November 26, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3