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P23371

- PAP1_VACCW

UniProt

P23371 - PAP1_VACCW

Protein

Poly(A) polymerase catalytic subunit

Gene

PAPL

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (07 Dec 2004)
      Previous versions | rss
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    Functioni

    Polymerase that creates the 3'-poly(A) tail of mRNA's.1 Publication

    Catalytic activityi

    ATP + RNA(n) = diphosphate + RNA(n+1).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei202 – 2021By similarity
    Active sitei204 – 2041By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. polynucleotide adenylyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mRNA processing Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    mRNA processing, Transcription

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Poly(A) polymerase catalytic subunit (EC:2.7.7.19)
    Alternative name(s):
    Poly(A) polymerase large subunit
    Short name:
    PAP-L
    VP55
    Gene namesi
    Name:PAPL
    Ordered Locus Names:VACWR057
    ORF Names:E1L
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000000344: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 479479Poly(A) polymerase catalytic subunitPRO_0000099107Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.

    Protein-protein interaction databases

    DIPiDIP-48309N.

    Structurei

    Secondary structure

    1
    479
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi13 – 2210
    Helixi28 – 347
    Helixi35 – 373
    Helixi38 – 469
    Helixi49 – 6315
    Helixi71 – 8111
    Helixi82 – 876
    Helixi91 – 11121
    Helixi113 – 1164
    Helixi131 – 1344
    Helixi135 – 14511
    Helixi165 – 17814
    Turni180 – 1823
    Beta strandi183 – 1875
    Helixi188 – 1925
    Beta strandi204 – 2096
    Helixi210 – 22516
    Beta strandi230 – 2334
    Beta strandi237 – 2448
    Beta strandi250 – 2567
    Helixi259 – 2646
    Beta strandi267 – 2704
    Beta strandi273 – 2764
    Helixi278 – 28811
    Helixi292 – 3009
    Helixi302 – 32019
    Beta strandi335 – 3384
    Turni339 – 3424
    Beta strandi343 – 3475
    Turni349 – 3513
    Beta strandi352 – 36110
    Helixi363 – 37210
    Helixi382 – 3843
    Beta strandi386 – 3927
    Beta strandi395 – 3995
    Beta strandi405 – 4084
    Helixi414 – 43017
    Helixi436 – 4449
    Beta strandi468 – 4714
    Turni472 – 4754
    Beta strandi476 – 4783

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2GA9X-ray2.30D11-479[»]
    2GAFX-ray2.40D11-479[»]
    3ER8X-ray3.18C/D1-479[»]
    3ER9X-ray2.06B1-479[»]
    3ERCX-ray3.21C/D1-479[»]
    3OWGX-ray2.86A/B1-479[»]
    ProteinModelPortaliP23371.
    SMRiP23371. Positions 12-479.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23371.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR024231. Pox_polyA_pol_nucTrfase.
    IPR004976. Poxvirus_polyA_pol_cat.
    IPR024397. Poxvirus_polyA_pol_cat_C.
    IPR024398. Poxvirus_polyA_pol_cat_N.
    [Graphical view]
    PfamiPF03296. Pox_polyA_pol. 1 hit.
    PF12629. Pox_polyA_pol_C. 1 hit.
    PF12630. Pox_polyA_pol_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015693. VAC-48L_nuct. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P23371-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD    50
    IFVSLVKKNK KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL 100
    QSVLVTTYTD ILGVLTIKAP NVISSKISYN VTSMEELARD MLNSMNVAVI 150
    DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN KSCICYGSYS LYLINPNIRY 200
    GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY MVIKDENDNH 250
    IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK 300
    DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD 350
    YFSFKKCLVY LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF 400
    SNTILLSDKG KVHEISARGL CAHILLYQML TSGEYKQCLS DLLNSMMNRD 450
    KIPIYSHTER DKKPGRHGFI NIEKDIIVF 479
    Length:479
    Mass (Da):55,531
    Last modified:December 7, 2004 - v2
    Checksum:i2E76F2BB1A246D5F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101L → F in AAB59821. 1 PublicationCurated
    Sequence conflicti68 – 681N → D in AAB59821. 1 PublicationCurated
    Sequence conflicti199 – 1991R → L in AAB59821. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36339 Genomic DNA. Translation: AAB59821.1.
    AY243312 Genomic DNA. Translation: AAO89336.1.
    RefSeqiYP_232939.1. NC_006998.1.

    Genome annotation databases

    GeneIDi3707514.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36339 Genomic DNA. Translation: AAB59821.1 .
    AY243312 Genomic DNA. Translation: AAO89336.1 .
    RefSeqi YP_232939.1. NC_006998.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2GA9 X-ray 2.30 D 11-479 [» ]
    2GAF X-ray 2.40 D 11-479 [» ]
    3ER8 X-ray 3.18 C/D 1-479 [» ]
    3ER9 X-ray 2.06 B 1-479 [» ]
    3ERC X-ray 3.21 C/D 1-479 [» ]
    3OWG X-ray 2.86 A/B 1-479 [» ]
    ProteinModelPortali P23371.
    SMRi P23371. Positions 12-479.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48309N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3707514.

    Miscellaneous databases

    EvolutionaryTracei P23371.

    Family and domain databases

    InterProi IPR024231. Pox_polyA_pol_nucTrfase.
    IPR004976. Poxvirus_polyA_pol_cat.
    IPR024397. Poxvirus_polyA_pol_cat_C.
    IPR024398. Poxvirus_polyA_pol_cat_N.
    [Graphical view ]
    Pfami PF03296. Pox_polyA_pol. 1 hit.
    PF12629. Pox_polyA_pol_C. 1 hit.
    PF12630. Pox_polyA_pol_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015693. VAC-48L_nuct. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Gershon P.D., Jones E.V., Moss B., Ahn B.-Y.
      Submitted (JUL-1990) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
      Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Poly(A) polymerase and a dissociable polyadenylation stimulatory factor encoded by vaccinia virus."
      Gershon P.D., Ahn B.-Y., Garfield M., Moss B.
      Cell 66:1269-1278(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPAP1_VACCW
    AccessioniPrimary (citable) accession number: P23371
    Secondary accession number(s): Q80HX2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3