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Protein

Poly(A) polymerase catalytic subunit

Gene

PAPL

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Polymerase that creates the 3'-poly(A) tail of mRNA's.1 Publication

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei202By similarity1
Active sitei204By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

mRNA processing, Transcription

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Poly(A) polymerase catalytic subunit (EC:2.7.7.19)
Alternative name(s):
Poly(A) polymerase large subunit
Short name:
PAP-L
VP55
Gene namesi
Name:PAPL
Ordered Locus Names:VACWR057
ORF Names:E1L
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000000344 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000991071 – 479Poly(A) polymerase catalytic subunitAdd BLAST479

Interactioni

Subunit structurei

Heterodimer of a large (catalytic) subunit and a small (regulatory) subunit.

Protein-protein interaction databases

DIPiDIP-48309N.

Structurei

Secondary structure

1479
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi13 – 22Combined sources10
Helixi28 – 34Combined sources7
Helixi35 – 37Combined sources3
Helixi38 – 46Combined sources9
Helixi49 – 63Combined sources15
Helixi71 – 81Combined sources11
Helixi82 – 87Combined sources6
Helixi91 – 111Combined sources21
Helixi113 – 116Combined sources4
Helixi131 – 134Combined sources4
Helixi135 – 145Combined sources11
Helixi165 – 178Combined sources14
Turni180 – 182Combined sources3
Beta strandi183 – 187Combined sources5
Helixi188 – 192Combined sources5
Beta strandi204 – 209Combined sources6
Helixi210 – 225Combined sources16
Beta strandi230 – 233Combined sources4
Beta strandi237 – 244Combined sources8
Beta strandi250 – 256Combined sources7
Helixi259 – 264Combined sources6
Beta strandi267 – 270Combined sources4
Beta strandi273 – 276Combined sources4
Helixi278 – 288Combined sources11
Helixi292 – 300Combined sources9
Helixi302 – 320Combined sources19
Beta strandi335 – 338Combined sources4
Turni339 – 342Combined sources4
Beta strandi343 – 347Combined sources5
Turni349 – 351Combined sources3
Beta strandi352 – 361Combined sources10
Helixi363 – 372Combined sources10
Helixi382 – 384Combined sources3
Beta strandi386 – 392Combined sources7
Beta strandi395 – 399Combined sources5
Beta strandi405 – 408Combined sources4
Helixi414 – 430Combined sources17
Helixi436 – 444Combined sources9
Beta strandi468 – 471Combined sources4
Turni472 – 475Combined sources4
Beta strandi476 – 478Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GA9X-ray2.30D11-479[»]
2GAFX-ray2.40D11-479[»]
3ER8X-ray3.18C/D1-479[»]
3ER9X-ray2.06B1-479[»]
3ERCX-ray3.21C/D1-479[»]
3OWGX-ray2.86A/B1-479[»]
ProteinModelPortaliP23371.
SMRiP23371.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23371.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR024231. Pox_polyA_pol_nucTrfase.
IPR024397. Poxvirus_polyA_pol_cat_C.
IPR024398. Poxvirus_polyA_pol_cat_N.
[Graphical view]
PfamiPF03296. Pox_polyA_pol. 1 hit.
PF12629. Pox_polyA_pol_C. 1 hit.
PF12630. Pox_polyA_pol_N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRNPDQNTL PNITLKIIET YLGRVPSVNE YHMLKLQARN IQKITVFNKD
60 70 80 90 100
IFVSLVKKNK KRFFSDVNTS ASEIKDRILS YFSKQTQTYN IGKLFTIIEL
110 120 130 140 150
QSVLVTTYTD ILGVLTIKAP NVISSKISYN VTSMEELARD MLNSMNVAVI
160 170 180 190 200
DKAKVMGRHN VSSLVKNVNK LMEEYLRRHN KSCICYGSYS LYLINPNIRY
210 220 230 240 250
GDIDILQTNS RTFLIDLAFL IKFITGNNII LSKIPYLRNY MVIKDENDNH
260 270 280 290 300
IIDSFNIRQD TMNVVPKIFI DNIYIVDPTF QLLNMIKMFS QIDRLEDLSK
310 320 330 340 350
DPEKFNARMA TMLEYVRYTH GIVFDGKRNN MPMKCIIDEN NRIVTVTTKD
360 370 380 390 400
YFSFKKCLVY LDENVLSSDI LDLNADTSCD FESVTNSVYL IHDNIMYTYF
410 420 430 440 450
SNTILLSDKG KVHEISARGL CAHILLYQML TSGEYKQCLS DLLNSMMNRD
460 470
KIPIYSHTER DKKPGRHGFI NIEKDIIVF
Length:479
Mass (Da):55,531
Last modified:December 7, 2004 - v2
Checksum:i2E76F2BB1A246D5F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10L → F in AAB59821 (Ref. 1) Curated1
Sequence conflicti68N → D in AAB59821 (Ref. 1) Curated1
Sequence conflicti199R → L in AAB59821 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36339 Genomic DNA. Translation: AAB59821.1.
AY243312 Genomic DNA. Translation: AAO89336.1.
RefSeqiYP_232939.1. NC_006998.1.

Genome annotation databases

GeneIDi3707514.
KEGGivg:3707514.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36339 Genomic DNA. Translation: AAB59821.1.
AY243312 Genomic DNA. Translation: AAO89336.1.
RefSeqiYP_232939.1. NC_006998.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GA9X-ray2.30D11-479[»]
2GAFX-ray2.40D11-479[»]
3ER8X-ray3.18C/D1-479[»]
3ER9X-ray2.06B1-479[»]
3ERCX-ray3.21C/D1-479[»]
3OWGX-ray2.86A/B1-479[»]
ProteinModelPortaliP23371.
SMRiP23371.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48309N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi3707514.
KEGGivg:3707514.

Miscellaneous databases

EvolutionaryTraceiP23371.

Family and domain databases

InterProiIPR024231. Pox_polyA_pol_nucTrfase.
IPR024397. Poxvirus_polyA_pol_cat_C.
IPR024398. Poxvirus_polyA_pol_cat_N.
[Graphical view]
PfamiPF03296. Pox_polyA_pol. 1 hit.
PF12629. Pox_polyA_pol_C. 1 hit.
PF12630. Pox_polyA_pol_N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAP1_VACCW
AccessioniPrimary (citable) accession number: P23371
Secondary accession number(s): Q80HX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.