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Protein

DNA mismatch repair protein MutL

Gene

mutL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a "molecular matchmaker", a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA.

GO - Molecular functioni

  • ATPase activity Source: EcoliWiki
  • ATP binding Source: EcoliWiki
  • DNA binding Source: EcoliWiki
  • mismatched DNA binding Source: InterPro
  • single-stranded DNA binding Source: GO_Central

GO - Biological processi

  • mismatch repair Source: EcoliWiki
  • regulation of DNA recombination Source: EcoliWiki
Complete GO annotation...

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11281-MONOMER.
ECOL316407:JW4128-MONOMER.
MetaCyc:EG11281-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA mismatch repair protein MutL
Gene namesi
Name:mutL
Ordered Locus Names:b4170, JW4128
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11281. mutL.

Subcellular locationi

GO - Cellular componenti

  • mismatch repair complex Source: EcoliWiki
Complete GO annotation...

Pathology & Biotechi

Chemistry databases

DrugBankiDB02325. Isopropyl Alcohol.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001779431 – 615DNA mismatch repair protein MutLAdd BLAST615

Proteomic databases

PaxDbiP23367.
PRIDEiP23367.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-554913,EBI-554913
dnaXP06710-22EBI-554913,EBI-2604194
holAP286302EBI-554913,EBI-549153
holBP286312EBI-554913,EBI-549161
mutHP067228EBI-554913,EBI-545170
recAP0A7G63EBI-554913,EBI-370331
uvrDP030187EBI-554913,EBI-559573
vsrP091843EBI-554913,EBI-765033

Protein-protein interaction databases

BioGridi4263476. 32 interactors.
DIPiDIP-10285N.
IntActiP23367. 13 interactors.
MINTiMINT-1266937.
STRINGi511145.b4170.

Structurei

Secondary structure

1615
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 19Combined sources12
Helixi23 – 36Combined sources14
Beta strandi40 – 47Combined sources8
Helixi48 – 50Combined sources3
Beta strandi52 – 58Combined sources7
Helixi65 – 67Combined sources3
Helixi68 – 72Combined sources5
Helixi83 – 87Combined sources5
Helixi90 – 92Combined sources3
Helixi98 – 103Combined sources6
Beta strandi106 – 113Combined sources8
Beta strandi115 – 117Combined sources3
Beta strandi118 – 126Combined sources9
Turni127 – 130Combined sources4
Beta strandi131 – 137Combined sources7
Beta strandi141 – 149Combined sources9
Turni150 – 153Combined sources4
Helixi155 – 159Combined sources5
Helixi164 – 181Combined sources18
Beta strandi186 – 191Combined sources6
Beta strandi194 – 199Combined sources6
Helixi209 – 216Combined sources8
Helixi218 – 223Combined sources6
Beta strandi224 – 231Combined sources8
Beta strandi234 – 241Combined sources8
Helixi243 – 245Combined sources3
Helixi250 – 252Combined sources3
Beta strandi254 – 258Combined sources5
Beta strandi261 – 263Combined sources3
Helixi266 – 279Combined sources14
Beta strandi280 – 282Combined sources3
Beta strandi288 – 293Combined sources6
Helixi296 – 298Combined sources3
Beta strandi311 – 314Combined sources4
Helixi315 – 329Combined sources15
Beta strandi437 – 442Combined sources6
Turni443 – 445Combined sources3
Beta strandi446 – 451Combined sources6
Beta strandi454 – 459Combined sources6
Helixi460 – 472Combined sources13
Beta strandi481 – 491Combined sources11
Helixi494 – 509Combined sources16
Beta strandi513 – 516Combined sources4
Beta strandi518 – 527Combined sources10
Helixi528 – 530Combined sources3
Helixi535 – 546Combined sources12
Helixi554 – 563Combined sources10
Helixi574 – 587Combined sources14
Helixi589 – 593Combined sources5
Turni597 – 599Combined sources3
Beta strandi600 – 602Combined sources3
Helixi606 – 613Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B62X-ray2.10A1-349[»]
1B63X-ray1.90A1-331[»]
1BKNX-ray2.90A/B1-349[»]
1NHHX-ray2.40A1-331[»]
1NHIX-ray2.00A1-331[»]
1NHJX-ray2.30A1-331[»]
1X9ZX-ray2.10A/B432-615[»]
5AKBX-ray4.71C/D/F1-349[»]
5AKCX-ray6.60C/D/G/H/K/L1-349[»]
5AKDX-ray7.60C/D/G/H/K/L1-349[»]
ProteinModelPortaliP23367.
SMRiP23367.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23367.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105DC6. Bacteria.
COG0323. LUCA.
HOGENOMiHOG000256551.
InParanoidiP23367.
KOiK03572.
OMAiWQQENRQ.
PhylomeDBiP23367.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPiMF_00149. DNA_mis_repair. 1 hit.
InterProiIPR013507. DNA_mismatch_repair_C.
IPR014762. DNA_mismatch_repair_CS.
IPR002099. DNA_mismatch_repair_fam.
IPR020667. DNA_mismatch_repair_MutL.
IPR003594. HATPase_C.
IPR014790. MutL_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF01119. DNA_mis_repair. 1 hit.
PF08676. MutL_C. 1 hit.
[Graphical view]
SMARTiSM01340. DNA_mis_repair. 1 hit.
SM00853. MutL_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00585. mutl. 1 hit.
PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIQVLPPQL ANQIAAGEVV ERPASVVKEL VENSLDAGAT RIDIDIERGG
60 70 80 90 100
AKLIRIRDNG CGIKKDELAL ALARHATSKI ASLDDLEAII SLGFRGEALA
110 120 130 140 150
SISSVSRLTL TSRTAEQQEA WQAYAEGRDM NVTVKPAAHP VGTTLEVLDL
160 170 180 190 200
FYNTPARRKF LRTEKTEFNH IDEIIRRIAL ARFDVTINLS HNGKIVRQYR
210 220 230 240 250
AVPEGGQKER RLGAICGTAF LEQALAIEWQ HGDLTLRGWV ADPNHTTPAL
260 270 280 290 300
AEIQYCYVNG RMMRDRLINH AIRQACEDKL GADQQPAFVL YLEIDPHQVD
310 320 330 340 350
VNVHPAKHEV RFHQSRLVHD FIYQGVLSVL QQQLETPLPL DDEPQPAPRS
360 370 380 390 400
IPENRVAAGR NHFAEPAARE PVAPRYTPAP ASGSRPAAPW PNAQPGYQKQ
410 420 430 440 450
QGEVYRQLLQ TPAPMQKLKA PEPQEPALAA NSQSFGRVLT IVHSDCALLE
460 470 480 490 500
RDGNISLLSL PVAERWLRQA QLTPGEAPVC AQPLLIPLRL KVSAEEKSAL
510 520 530 540 550
EKAQSALAEL GIDFQSDAQH VTIRAVPLPL RQQNLQILIP ELIGYLAKQS
560 570 580 590 600
VFEPGNIAQW IARNLMSEHA QWSMAQAITL LADVERLCPQ LVKTPPGGLL
610
QSVDLHPAIK ALKDE
Length:615
Mass (Da):67,924
Last modified:May 1, 1992 - v2
Checksum:iC69D2735BF5FA165
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11831 Genomic DNA. Translation: CAA77850.1.
U14003 Genomic DNA. Translation: AAA97066.1.
U00096 Genomic DNA. Translation: AAC77127.1.
AP009048 Genomic DNA. Translation: BAE78171.1.
M63655 Genomic DNA. Translation: AAA24173.1.
L19346 Unassigned DNA. Translation: AAA20098.1.
PIRiPH0853.
RefSeqiNP_418591.1. NC_000913.3.
WP_001122505.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77127; AAC77127; b4170.
BAE78171; BAE78171; BAE78171.
GeneIDi948691.
KEGGiecj:JW4128.
eco:b4170.
PATRICi32123913. VBIEscCol129921_4301.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11831 Genomic DNA. Translation: CAA77850.1.
U14003 Genomic DNA. Translation: AAA97066.1.
U00096 Genomic DNA. Translation: AAC77127.1.
AP009048 Genomic DNA. Translation: BAE78171.1.
M63655 Genomic DNA. Translation: AAA24173.1.
L19346 Unassigned DNA. Translation: AAA20098.1.
PIRiPH0853.
RefSeqiNP_418591.1. NC_000913.3.
WP_001122505.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B62X-ray2.10A1-349[»]
1B63X-ray1.90A1-331[»]
1BKNX-ray2.90A/B1-349[»]
1NHHX-ray2.40A1-331[»]
1NHIX-ray2.00A1-331[»]
1NHJX-ray2.30A1-331[»]
1X9ZX-ray2.10A/B432-615[»]
5AKBX-ray4.71C/D/F1-349[»]
5AKCX-ray6.60C/D/G/H/K/L1-349[»]
5AKDX-ray7.60C/D/G/H/K/L1-349[»]
ProteinModelPortaliP23367.
SMRiP23367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263476. 32 interactors.
DIPiDIP-10285N.
IntActiP23367. 13 interactors.
MINTiMINT-1266937.
STRINGi511145.b4170.

Chemistry databases

DrugBankiDB02325. Isopropyl Alcohol.

Proteomic databases

PaxDbiP23367.
PRIDEiP23367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77127; AAC77127; b4170.
BAE78171; BAE78171; BAE78171.
GeneIDi948691.
KEGGiecj:JW4128.
eco:b4170.
PATRICi32123913. VBIEscCol129921_4301.

Organism-specific databases

EchoBASEiEB1258.
EcoGeneiEG11281. mutL.

Phylogenomic databases

eggNOGiENOG4105DC6. Bacteria.
COG0323. LUCA.
HOGENOMiHOG000256551.
InParanoidiP23367.
KOiK03572.
OMAiWQQENRQ.
PhylomeDBiP23367.

Enzyme and pathway databases

BioCyciEcoCyc:EG11281-MONOMER.
ECOL316407:JW4128-MONOMER.
MetaCyc:EG11281-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP23367.
PROiP23367.

Family and domain databases

Gene3Di3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPiMF_00149. DNA_mis_repair. 1 hit.
InterProiIPR013507. DNA_mismatch_repair_C.
IPR014762. DNA_mismatch_repair_CS.
IPR002099. DNA_mismatch_repair_fam.
IPR020667. DNA_mismatch_repair_MutL.
IPR003594. HATPase_C.
IPR014790. MutL_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PfamiPF01119. DNA_mis_repair. 1 hit.
PF08676. MutL_C. 1 hit.
[Graphical view]
SMARTiSM01340. DNA_mis_repair. 1 hit.
SM00853. MutL_C. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00585. mutl. 1 hit.
PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMUTL_ECOLI
AccessioniPrimary (citable) accession number: P23367
Secondary accession number(s): Q2M6D5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 1, 1992
Last modified: November 2, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.