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P23367

- MUTL_ECOLI

UniProt

P23367 - MUTL_ECOLI

Protein

DNA mismatch repair protein MutL

Gene

mutL

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 2 (01 May 1992)
      Previous versions | rss
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    Functioni

    This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a "molecular matchmaker", a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA.

    GO - Molecular functioni

    1. ATPase activity Source: EcoliWiki
    2. ATP binding Source: EcoliWiki
    3. DNA binding Source: EcoliWiki
    4. identical protein binding Source: IntAct
    5. mismatched DNA binding Source: InterPro
    6. protein binding Source: IntAct
    7. single-stranded DNA binding Source: RefGenome

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. mismatch repair Source: EcoliWiki
    3. regulation of DNA recombination Source: EcoliWiki

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11281-MONOMER.
    ECOL316407:JW4128-MONOMER.
    MetaCyc:EG11281-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA mismatch repair protein MutL
    Gene namesi
    Name:mutL
    Ordered Locus Names:b4170, JW4128
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11281. mutL.

    Subcellular locationi

    GO - Cellular componenti

    1. mismatch repair complex Source: EcoliWiki
    2. nucleus Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 615615DNA mismatch repair protein MutLPRO_0000177943Add
    BLAST

    Proteomic databases

    PaxDbiP23367.
    PRIDEiP23367.

    Expressioni

    Gene expression databases

    GenevestigatoriP23367.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-554913,EBI-554913
    holAP286302EBI-554913,EBI-549153
    holBP286312EBI-554913,EBI-549161
    mutHP067228EBI-554913,EBI-545170
    recAP0A7G63EBI-554913,EBI-370331
    uvrDP030187EBI-554913,EBI-559573
    vsrP091843EBI-554913,EBI-765033

    Protein-protein interaction databases

    DIPiDIP-10285N.
    IntActiP23367. 12 interactions.
    MINTiMINT-1266937.
    STRINGi511145.b4170.

    Structurei

    Secondary structure

    1
    615
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi8 – 1912
    Helixi23 – 3614
    Beta strandi40 – 478
    Helixi48 – 503
    Beta strandi52 – 587
    Helixi65 – 673
    Helixi68 – 725
    Helixi83 – 875
    Helixi90 – 923
    Helixi98 – 1036
    Beta strandi106 – 1138
    Beta strandi115 – 1173
    Beta strandi118 – 1269
    Turni127 – 1304
    Beta strandi131 – 1377
    Beta strandi141 – 1499
    Turni150 – 1534
    Helixi155 – 1595
    Helixi164 – 18118
    Beta strandi186 – 1916
    Beta strandi194 – 1996
    Helixi209 – 2168
    Helixi218 – 2236
    Beta strandi224 – 2318
    Beta strandi234 – 2418
    Helixi243 – 2453
    Helixi250 – 2523
    Beta strandi254 – 2585
    Beta strandi261 – 2633
    Helixi266 – 27914
    Beta strandi280 – 2823
    Beta strandi288 – 2936
    Helixi296 – 2983
    Beta strandi311 – 3144
    Helixi315 – 32915
    Beta strandi437 – 4426
    Turni443 – 4453
    Beta strandi446 – 4516
    Beta strandi454 – 4596
    Helixi460 – 47213
    Beta strandi481 – 49111
    Helixi494 – 50916
    Beta strandi513 – 5164
    Beta strandi518 – 52710
    Helixi528 – 5303
    Helixi535 – 54612
    Helixi554 – 56310
    Helixi574 – 58714
    Helixi589 – 5935
    Turni597 – 5993
    Beta strandi600 – 6023
    Helixi606 – 6138

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1B62X-ray2.10A1-349[»]
    1B63X-ray1.90A1-331[»]
    1BKNX-ray2.90A/B1-349[»]
    1NHHX-ray2.40A1-331[»]
    1NHIX-ray2.00A1-331[»]
    1NHJX-ray2.30A1-331[»]
    1X9ZX-ray2.10A/B432-615[»]
    ProteinModelPortaliP23367.
    SMRiP23367. Positions 1-331, 433-614.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23367.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0323.
    HOGENOMiHOG000256551.
    KOiK03572.
    OMAiFSHIDEI.
    OrthoDBiEOG6P8TMH.
    PhylomeDBiP23367.

    Family and domain databases

    Gene3Di3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    HAMAPiMF_00149. DNA_mis_repair.
    InterProiIPR013507. DNA_mismatch_repair_C.
    IPR014762. DNA_mismatch_repair_CS.
    IPR002099. DNA_mismatch_repair_fam.
    IPR011186. DNA_mismatch_repair_MLH1/HexB.
    IPR020667. DNA_mismatch_repair_MutL.
    IPR003594. HATPase_ATP-bd.
    IPR014790. MutL_C.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view]
    PANTHERiPTHR10073:SF12. PTHR10073:SF12. 1 hit.
    PfamiPF01119. DNA_mis_repair. 1 hit.
    PF08676. MutL_C. 1 hit.
    [Graphical view]
    SMARTiSM00387. HATPase_c. 1 hit.
    SM00853. MutL_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    TIGRFAMsiTIGR00585. mutl. 1 hit.
    PROSITEiPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23367-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPIQVLPPQL ANQIAAGEVV ERPASVVKEL VENSLDAGAT RIDIDIERGG    50
    AKLIRIRDNG CGIKKDELAL ALARHATSKI ASLDDLEAII SLGFRGEALA 100
    SISSVSRLTL TSRTAEQQEA WQAYAEGRDM NVTVKPAAHP VGTTLEVLDL 150
    FYNTPARRKF LRTEKTEFNH IDEIIRRIAL ARFDVTINLS HNGKIVRQYR 200
    AVPEGGQKER RLGAICGTAF LEQALAIEWQ HGDLTLRGWV ADPNHTTPAL 250
    AEIQYCYVNG RMMRDRLINH AIRQACEDKL GADQQPAFVL YLEIDPHQVD 300
    VNVHPAKHEV RFHQSRLVHD FIYQGVLSVL QQQLETPLPL DDEPQPAPRS 350
    IPENRVAAGR NHFAEPAARE PVAPRYTPAP ASGSRPAAPW PNAQPGYQKQ 400
    QGEVYRQLLQ TPAPMQKLKA PEPQEPALAA NSQSFGRVLT IVHSDCALLE 450
    RDGNISLLSL PVAERWLRQA QLTPGEAPVC AQPLLIPLRL KVSAEEKSAL 500
    EKAQSALAEL GIDFQSDAQH VTIRAVPLPL RQQNLQILIP ELIGYLAKQS 550
    VFEPGNIAQW IARNLMSEHA QWSMAQAITL LADVERLCPQ LVKTPPGGLL 600
    QSVDLHPAIK ALKDE 615
    Length:615
    Mass (Da):67,924
    Last modified:May 1, 1992 - v2
    Checksum:iC69D2735BF5FA165
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11831 Genomic DNA. Translation: CAA77850.1.
    U14003 Genomic DNA. Translation: AAA97066.1.
    U00096 Genomic DNA. Translation: AAC77127.1.
    AP009048 Genomic DNA. Translation: BAE78171.1.
    M63655 Genomic DNA. Translation: AAA24173.1.
    L19346 Unassigned DNA. Translation: AAA20098.1.
    PIRiPH0853.
    RefSeqiNP_418591.1. NC_000913.3.
    YP_492312.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC77127; AAC77127; b4170.
    BAE78171; BAE78171; BAE78171.
    GeneIDi12934482.
    948691.
    KEGGiecj:Y75_p4056.
    eco:b4170.
    PATRICi32123913. VBIEscCol129921_4301.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11831 Genomic DNA. Translation: CAA77850.1 .
    U14003 Genomic DNA. Translation: AAA97066.1 .
    U00096 Genomic DNA. Translation: AAC77127.1 .
    AP009048 Genomic DNA. Translation: BAE78171.1 .
    M63655 Genomic DNA. Translation: AAA24173.1 .
    L19346 Unassigned DNA. Translation: AAA20098.1 .
    PIRi PH0853.
    RefSeqi NP_418591.1. NC_000913.3.
    YP_492312.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1B62 X-ray 2.10 A 1-349 [» ]
    1B63 X-ray 1.90 A 1-331 [» ]
    1BKN X-ray 2.90 A/B 1-349 [» ]
    1NHH X-ray 2.40 A 1-331 [» ]
    1NHI X-ray 2.00 A 1-331 [» ]
    1NHJ X-ray 2.30 A 1-331 [» ]
    1X9Z X-ray 2.10 A/B 432-615 [» ]
    ProteinModelPortali P23367.
    SMRi P23367. Positions 1-331, 433-614.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-10285N.
    IntActi P23367. 12 interactions.
    MINTi MINT-1266937.
    STRINGi 511145.b4170.

    Proteomic databases

    PaxDbi P23367.
    PRIDEi P23367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC77127 ; AAC77127 ; b4170 .
    BAE78171 ; BAE78171 ; BAE78171 .
    GeneIDi 12934482.
    948691.
    KEGGi ecj:Y75_p4056.
    eco:b4170.
    PATRICi 32123913. VBIEscCol129921_4301.

    Organism-specific databases

    EchoBASEi EB1258.
    EcoGenei EG11281. mutL.

    Phylogenomic databases

    eggNOGi COG0323.
    HOGENOMi HOG000256551.
    KOi K03572.
    OMAi FSHIDEI.
    OrthoDBi EOG6P8TMH.
    PhylomeDBi P23367.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11281-MONOMER.
    ECOL316407:JW4128-MONOMER.
    MetaCyc:EG11281-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P23367.
    PROi P23367.

    Gene expression databases

    Genevestigatori P23367.

    Family and domain databases

    Gene3Di 3.30.230.10. 1 hit.
    3.30.565.10. 1 hit.
    HAMAPi MF_00149. DNA_mis_repair.
    InterProi IPR013507. DNA_mismatch_repair_C.
    IPR014762. DNA_mismatch_repair_CS.
    IPR002099. DNA_mismatch_repair_fam.
    IPR011186. DNA_mismatch_repair_MLH1/HexB.
    IPR020667. DNA_mismatch_repair_MutL.
    IPR003594. HATPase_ATP-bd.
    IPR014790. MutL_C.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
    [Graphical view ]
    PANTHERi PTHR10073:SF12. PTHR10073:SF12. 1 hit.
    Pfami PF01119. DNA_mis_repair. 1 hit.
    PF08676. MutL_C. 1 hit.
    [Graphical view ]
    SMARTi SM00387. HATPase_c. 1 hit.
    SM00853. MutL_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    TIGRFAMsi TIGR00585. mutl. 1 hit.
    PROSITEi PS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium."
      Tsui H.-C.T., Mandavilli B.S., Winkler M.E.
      Nucleic Acids Res. 20:2379-2379(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
      Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
      Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations."
      Winkler M.E., Connolly D.M.
      J. Bacteriol. 173:1711-1721(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 596-615.
      Strain: K12.
    6. "The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase."
      Tsui H.-C.T., Zhao G., Feng G., Leung H.-C.E., Winkler M.E.
      Mol. Microbiol. 11:189-202(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
      Strain: K12.
    7. "Crystal structure and ATPase activity of MutL: implications for DNA repair and mutagenesis."
      Ban C., Yang W.
      Cell 95:541-552(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 1-349.
    8. "Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repair."
      Ban C., Junop M., Yang W.
      Cell 97:85-97(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-349.
      Strain: K12.

    Entry informationi

    Entry nameiMUTL_ECOLI
    AccessioniPrimary (citable) accession number: P23367
    Secondary accession number(s): Q2M6D5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 135 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3