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P23367 (MUTL_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 131. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA mismatch repair protein MutL
Gene names
Name:mutL
Ordered Locus Names:b4170, JW4128
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length615 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein is involved in the repair of mismatches in DNA. It is required for dam-dependent methyl-directed DNA mismatch repair. May act as a "molecular matchmaker", a protein that promotes the formation of a stable complex between two or more DNA-binding proteins in an ATP-dependent manner without itself being part of the final effector complex. The ATPase activity of MutL is stimulated by DNA. HAMAP-Rule MF_00149

Sequence similarities

Belongs to the DNA mismatch repair MutL/HexB family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 615615DNA mismatch repair protein MutL HAMAP-Rule MF_00149
PRO_0000177943

Secondary structure

........................................................................................... 615
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23367 [UniParc].

Last modified May 1, 1992. Version 2.
Checksum: C69D2735BF5FA165

FASTA61567,924
        10         20         30         40         50         60 
MPIQVLPPQL ANQIAAGEVV ERPASVVKEL VENSLDAGAT RIDIDIERGG AKLIRIRDNG 

        70         80         90        100        110        120 
CGIKKDELAL ALARHATSKI ASLDDLEAII SLGFRGEALA SISSVSRLTL TSRTAEQQEA 

       130        140        150        160        170        180 
WQAYAEGRDM NVTVKPAAHP VGTTLEVLDL FYNTPARRKF LRTEKTEFNH IDEIIRRIAL 

       190        200        210        220        230        240 
ARFDVTINLS HNGKIVRQYR AVPEGGQKER RLGAICGTAF LEQALAIEWQ HGDLTLRGWV 

       250        260        270        280        290        300 
ADPNHTTPAL AEIQYCYVNG RMMRDRLINH AIRQACEDKL GADQQPAFVL YLEIDPHQVD 

       310        320        330        340        350        360 
VNVHPAKHEV RFHQSRLVHD FIYQGVLSVL QQQLETPLPL DDEPQPAPRS IPENRVAAGR 

       370        380        390        400        410        420 
NHFAEPAARE PVAPRYTPAP ASGSRPAAPW PNAQPGYQKQ QGEVYRQLLQ TPAPMQKLKA 

       430        440        450        460        470        480 
PEPQEPALAA NSQSFGRVLT IVHSDCALLE RDGNISLLSL PVAERWLRQA QLTPGEAPVC 

       490        500        510        520        530        540 
AQPLLIPLRL KVSAEEKSAL EKAQSALAEL GIDFQSDAQH VTIRAVPLPL RQQNLQILIP 

       550        560        570        580        590        600 
ELIGYLAKQS VFEPGNIAQW IARNLMSEHA QWSMAQAITL LADVERLCPQ LVKTPPGGLL 

       610 
QSVDLHPAIK ALKDE 

« Hide

References

« Hide 'large scale' references
[1]"Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium."
Tsui H.-C.T., Mandavilli B.S., Winkler M.E.
Nucleic Acids Res. 20:2379-2379(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes."
Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 23:2105-2119(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Structure of Escherichia coli K-12 miaA and characterization of the mutator phenotype caused by miaA insertion mutations."
Winkler M.E., Connolly D.M.
J. Bacteriol. 173:1711-1721(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 596-615.
Strain: K12.
[6]"The mutL repair gene of Escherichia coli K-12 forms a superoperon with a gene encoding a new cell-wall amidase."
Tsui H.-C.T., Zhao G., Feng G., Leung H.-C.E., Winkler M.E.
Mol. Microbiol. 11:189-202(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
Strain: K12.
[7]"Crystal structure and ATPase activity of MutL: implications for DNA repair and mutagenesis."
Ban C., Yang W.
Cell 95:541-552(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 1-349.
[8]"Transformation of MutL by ATP binding and hydrolysis: a switch in DNA mismatch repair."
Ban C., Junop M., Yang W.
Cell 97:85-97(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 1-349.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11831 Genomic DNA. Translation: CAA77850.1.
U14003 Genomic DNA. Translation: AAA97066.1.
U00096 Genomic DNA. Translation: AAC77127.1.
AP009048 Genomic DNA. Translation: BAE78171.1.
M63655 Genomic DNA. Translation: AAA24173.1.
L19346 Unassigned DNA. Translation: AAA20098.1.
PIRPH0853.
RefSeqNP_418591.1. NC_000913.3.
YP_492312.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B62X-ray2.10A1-349[»]
1B63X-ray1.90A1-331[»]
1BKNX-ray2.90A/B1-349[»]
1NHHX-ray2.40A1-331[»]
1NHIX-ray2.00A1-331[»]
1NHJX-ray2.30A1-331[»]
1X9ZX-ray2.10A/B432-615[»]
ProteinModelPortalP23367.
SMRP23367. Positions 1-331, 433-614.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-10285N.
IntActP23367. 12 interactions.
MINTMINT-1266937.
STRING511145.b4170.

Proteomic databases

PaxDbP23367.
PRIDEP23367.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC77127; AAC77127; b4170.
BAE78171; BAE78171; BAE78171.
GeneID12934482.
948691.
KEGGecj:Y75_p4056.
eco:b4170.
PATRIC32123913. VBIEscCol129921_4301.

Organism-specific databases

EchoBASEEB1258.
EcoGeneEG11281. mutL.

Phylogenomic databases

eggNOGCOG0323.
HOGENOMHOG000256551.
KOK03572.
OMAFSHIDEI.
OrthoDBEOG6P8TMH.
PhylomeDBP23367.
ProtClustDBPRK00095.

Enzyme and pathway databases

BioCycEcoCyc:EG11281-MONOMER.
ECOL316407:JW4128-MONOMER.
MetaCyc:EG11281-MONOMER.

Gene expression databases

GenevestigatorP23367.

Family and domain databases

Gene3D3.30.230.10. 1 hit.
3.30.565.10. 1 hit.
HAMAPMF_00149. DNA_mis_repair.
InterProIPR013507. DNA_mismatch_repair_C.
IPR014762. DNA_mismatch_repair_CS.
IPR002099. DNA_mismatch_repair_fam.
IPR020667. DNA_mismatch_repair_MutL.
IPR003594. HATPase_ATP-bd.
IPR014790. MutL_C.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
[Graphical view]
PANTHERPTHR10073. PTHR10073. 1 hit.
PfamPF01119. DNA_mis_repair. 1 hit.
PF08676. MutL_C. 1 hit.
[Graphical view]
SMARTSM00387. HATPase_c. 1 hit.
SM00853. MutL_C. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsTIGR00585. mutl. 1 hit.
PROSITEPS00058. DNA_MISMATCH_REPAIR_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP23367.
PROP23367.

Entry information

Entry nameMUTL_ECOLI
AccessionPrimary (citable) accession number: P23367
Secondary accession number(s): Q2M6D5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene