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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

UCHL1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).By similarity

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei119NucleophilePROSITE-ProRule annotation1
Active sitei190Proton donorPROSITE-ProRule annotation1
Sitei205Important for enzyme activity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ligase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-BTA-5689603 UCH proteinases

Protein family/group databases

MEROPSiC12.001

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name:
PGP9.5
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 6

Organism-specific databases

VGNCiVGNC:36631 UCHL1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002110531 – 249Ubiquitin carboxyl-terminal hydrolase isozyme L1Add BLAST249
PropeptideiPRO_0000414309250 – 252Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei154PhosphoserineBy similarity1
Lipidationi249S-farnesyl cysteineBy similarity1

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP23356
PeptideAtlasiP23356
PRIDEiP23356

Expressioni

Tissue specificityi

Neurons and cells of the diffuse neuroendocrine system and their tumors.

Gene expression databases

BgeeiENSBTAG00000005078

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044075

Structurei

3D structure databases

ProteinModelPortaliP23356
SMRiP23356
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 10Interaction with ubiquitinBy similarity6
Regioni240 – 245Interaction with ubiquitinBy similarity6

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiKOG1415 Eukaryota
ENOG4111HNA LUCA
GeneTreeiENSGT00510000046640
HOGENOMiHOG000182400
HOVERGENiHBG075483
InParanoidiP23356
KOiK05611
OMAiAIQEVHN
OrthoDBiEOG091G0LBS
TreeFamiTF316166

Family and domain databases

Gene3Di3.40.532.10, 1 hit
InterProiView protein in InterPro
IPR001578 Peptidase_C12_UCH
IPR036959 Peptidase_C12_UCH_sf
IPR030297 UCHL1
PANTHERiPTHR10589 PTHR10589, 1 hit
PTHR10589:SF19 PTHR10589:SF19, 1 hit
PfamiView protein in Pfam
PF01088 Peptidase_C12, 1 hit
PRINTSiPR00707 UBCTHYDRLASE
PROSITEiView protein in PROSITE
PS00140 UCH_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLKPMEINP EMLNKVLTRL GVAGQWRFED VLGLEEESLG SVPAPACALL
60 70 80 90 100
LLFPLTAQRC FKLGREAASR FHHPDYPGRL FILLVSQHEN FRKKQIEELK
110 120 130 140 150
GQEVSPKVYF MKQTIGNSCG TIGLIHAVAN NQDKLEFEDG SVLKQFLSET
160 170 180 190 200
EKLSPEDRAK CFEKNEAIQA AHDAVAQEGQ CRVDDKVNFH FILFNNVDGH
210 220 230 240 250
LYELDGRMPF PVNHGTSSED SLLQDAAKVC REFTEREQGE VRFSAVALCK

AA
Length:252
Mass (Da):28,335
Last modified:June 13, 2006 - v2
Checksum:i9D62A458590188B7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9N → D AA sequence (PubMed:1833240).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111330 mRNA Translation: AAI11331.1
PIRiS17561
RefSeqiNP_001039637.1, NM_001046172.2
UniGeneiBt.58848

Genome annotation databases

EnsembliENSBTAT00000046823; ENSBTAP00000044075; ENSBTAG00000005078
GeneIDi514394
KEGGibta:514394

Similar proteinsi

Entry informationi

Entry nameiUCHL1_BOVIN
AccessioniPrimary (citable) accession number: P23356
Secondary accession number(s): Q2NKZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: June 13, 2006
Last modified: March 28, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health