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P23356

- UCHL1_BOVIN

UniProt

P23356 - UCHL1_BOVIN

Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

UCHL1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei1 – 11Susceptible to oxidationBy similarity
    Sitei6 – 61Susceptible to oxidationBy similarity
    Sitei12 – 121Susceptible to oxidationBy similarity
    Active sitei119 – 1191NucleophilePROSITE-ProRule annotation
    Active sitei190 – 1901Proton donorPROSITE-ProRule annotation
    Sitei205 – 2051Important for enzyme activity
    Sitei208 – 2081Susceptible to oxidationBy similarity
    Sitei249 – 2491Susceptible to oxidationBy similarity

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: Ensembl
    2. ligase activity Source: UniProtKB-KW
    3. omega peptidase activity Source: Ensembl
    4. ubiquitin-specific protease activity Source: InterPro
    5. ubiquitin thiolesterase activity Source: Ensembl

    GO - Biological processi

    1. adult walking behavior Source: Ensembl
    2. axon target recognition Source: Ensembl
    3. axon transport of mitochondrion Source: Ensembl
    4. cell proliferation Source: Ensembl
    5. eating behavior Source: Ensembl
    6. muscle fiber development Source: Ensembl
    7. negative regulation of MAP kinase activity Source: Ensembl
    8. neuromuscular process Source: Ensembl
    9. protein deubiquitination Source: Ensembl
    10. response to ischemia Source: Ensembl
    11. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Ligase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Protein family/group databases

    MEROPSiC12.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
    Short name:
    UCH-L1
    Alternative name(s):
    Neuron cytoplasmic protein 9.5
    PGP 9.5
    Short name:
    PGP9.5
    Ubiquitin thioesterase L1
    Gene namesi
    Name:UCHL1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 6

    Subcellular locationi

    GO - Cellular componenti

    1. axon Source: Ensembl
    2. cytosol Source: Ensembl
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. neuronal cell body Source: Ensembl
    5. nucleus Source: Ensembl
    6. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 249249Ubiquitin carboxyl-terminal hydrolase isozyme L1PRO_0000211053Add
    BLAST
    Propeptidei250 – 2523Removed in mature formBy similarityPRO_0000414309

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi249 – 2491S-farnesyl cysteineBy similarity

    Post-translational modificationi

    O-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein, Lipoprotein, Oxidation, Prenylation

    Proteomic databases

    PaxDbiP23356.
    PRIDEiP23356.

    Expressioni

    Tissue specificityi

    Neurons and cells of the diffuse neuroendocrine system and their tumors.

    Interactioni

    Subunit structurei

    Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000044075.

    Structurei

    3D structure databases

    ProteinModelPortaliP23356.
    SMRiP23356. Positions 1-252.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni5 – 106Interaction with ubiquitinBy similarity
    Regioni180 – 1856Interaction with ubiquitinBy similarity
    Regioni240 – 2456Interaction with ubiquitinBy similarity

    Sequence similaritiesi

    Belongs to the peptidase C12 family.Curated

    Phylogenomic databases

    eggNOGiNOG327708.
    GeneTreeiENSGT00510000046640.
    HOGENOMiHOG000182400.
    HOVERGENiHBG075483.
    InParanoidiP23356.
    KOiK05611.
    OMAiKSHENGH.
    OrthoDBiEOG7S7SFK.
    TreeFamiTF316166.

    Family and domain databases

    Gene3Di3.40.532.10. 1 hit.
    InterProiIPR001578. Peptidase_C12_UCH.
    [Graphical view]
    PANTHERiPTHR10589. PTHR10589. 1 hit.
    PfamiPF01088. Peptidase_C12. 1 hit.
    [Graphical view]
    PRINTSiPR00707. UBCTHYDRLASE.
    PROSITEiPS00140. UCH_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23356-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQLKPMEINP EMLNKVLTRL GVAGQWRFED VLGLEEESLG SVPAPACALL    50
    LLFPLTAQRC FKLGREAASR FHHPDYPGRL FILLVSQHEN FRKKQIEELK 100
    GQEVSPKVYF MKQTIGNSCG TIGLIHAVAN NQDKLEFEDG SVLKQFLSET 150
    EKLSPEDRAK CFEKNEAIQA AHDAVAQEGQ CRVDDKVNFH FILFNNVDGH 200
    LYELDGRMPF PVNHGTSSED SLLQDAAKVC REFTEREQGE VRFSAVALCK 250
    AA 252
    Length:252
    Mass (Da):28,335
    Last modified:June 13, 2006 - v2
    Checksum:i9D62A458590188B7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti9 – 91N → D AA sequence (PubMed:1833240)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC111330 mRNA. Translation: AAI11331.1.
    PIRiS17561.
    RefSeqiNP_001039637.1. NM_001046172.2.
    UniGeneiBt.58848.

    Genome annotation databases

    EnsembliENSBTAT00000046823; ENSBTAP00000044075; ENSBTAG00000005078.
    GeneIDi514394.
    KEGGibta:514394.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC111330 mRNA. Translation: AAI11331.1 .
    PIRi S17561.
    RefSeqi NP_001039637.1. NM_001046172.2.
    UniGenei Bt.58848.

    3D structure databases

    ProteinModelPortali P23356.
    SMRi P23356. Positions 1-252.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000044075.

    Protein family/group databases

    MEROPSi C12.001.

    Proteomic databases

    PaxDbi P23356.
    PRIDEi P23356.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000046823 ; ENSBTAP00000044075 ; ENSBTAG00000005078 .
    GeneIDi 514394.
    KEGGi bta:514394.

    Organism-specific databases

    CTDi 7345.

    Phylogenomic databases

    eggNOGi NOG327708.
    GeneTreei ENSGT00510000046640.
    HOGENOMi HOG000182400.
    HOVERGENi HBG075483.
    InParanoidi P23356.
    KOi K05611.
    OMAi KSHENGH.
    OrthoDBi EOG7S7SFK.
    TreeFami TF316166.

    Miscellaneous databases

    NextBioi 20871312.

    Family and domain databases

    Gene3Di 3.40.532.10. 1 hit.
    InterProi IPR001578. Peptidase_C12_UCH.
    [Graphical view ]
    PANTHERi PTHR10589. PTHR10589. 1 hit.
    Pfami PF01088. Peptidase_C12. 1 hit.
    [Graphical view ]
    PRINTSi PR00707. UBCTHYDRLASE.
    PROSITEi PS00140. UCH_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Liver.
    2. "'Neuron-specific' protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state."
      Giambanco I., Bianchi R., Ceccarelli P., Pula G., Sorci G., Antonioli S., Bocchini V., Donato R.
      FEBS Lett. 290:131-134(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-25.
      Tissue: Brain.

    Entry informationi

    Entry nameiUCHL1_BOVIN
    AccessioniPrimary (citable) accession number: P23356
    Secondary accession number(s): Q2NKZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3