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P23356 (UCHL1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1

Short name=UCH-L1
EC=3.4.19.12
EC=6.-.-.-
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name=PGP9.5
Ubiquitin thioesterase L1
Gene names
Name:UCHL1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length252 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Monomer. Homodimer. Interacts with COPS5 and SNCA By similarity.

Subcellular location

Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor By similarity.

Tissue specificity

Neurons and cells of the diffuse neuroendocrine system and their tumors.

Post-translational modification

O-glycosylated By similarity.

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8 By similarity.

Sequence similarities

Belongs to the peptidase C12 family.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentCytoplasm
Endoplasmic reticulum
Membrane
   Molecular functionHydrolase
Ligase
Protease
Thiol protease
   PTMGlycoprotein
Lipoprotein
Oxidation
Prenylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processadult walking behavior

Inferred from electronic annotation. Source: Ensembl

axon target recognition

Inferred from electronic annotation. Source: Ensembl

axon transport of mitochondrion

Inferred from electronic annotation. Source: Ensembl

cell proliferation

Inferred from electronic annotation. Source: Ensembl

eating behavior

Inferred from electronic annotation. Source: Ensembl

muscle fiber development

Inferred from electronic annotation. Source: Ensembl

negative regulation of MAP kinase activity

Inferred from electronic annotation. Source: Ensembl

neuromuscular process

Inferred from electronic annotation. Source: Ensembl

protein deubiquitination

Inferred from electronic annotation. Source: Ensembl

response to ischemia

Inferred from electronic annotation. Source: Ensembl

ubiquitin-dependent protein catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentaxon

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_functioncysteine-type endopeptidase activity

Inferred from electronic annotation. Source: Ensembl

ligase activity

Inferred from electronic annotation. Source: UniProtKB-KW

omega peptidase activity

Inferred from electronic annotation. Source: Ensembl

ubiquitin thiolesterase activity

Inferred from electronic annotation. Source: Ensembl

ubiquitin-specific protease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 249249Ubiquitin carboxyl-terminal hydrolase isozyme L1
PRO_0000211053
Propeptide250 – 2523Removed in mature form By similarity
PRO_0000414309

Regions

Region5 – 106Interaction with ubiquitin By similarity
Region180 – 1856Interaction with ubiquitin By similarity
Region240 – 2456Interaction with ubiquitin By similarity

Sites

Active site1191Nucleophile By similarity
Active site1901Proton donor By similarity
Site11Susceptible to oxidation By similarity
Site61Susceptible to oxidation By similarity
Site121Susceptible to oxidation By similarity
Site2051Important for enzyme activity
Site2081Susceptible to oxidation By similarity
Site2491Susceptible to oxidation By similarity

Amino acid modifications

Lipidation2491S-farnesyl cysteine By similarity

Experimental info

Sequence conflict91N → D AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P23356 [UniParc].

Last modified June 13, 2006. Version 2.
Checksum: 9D62A458590188B7

FASTA25228,335
        10         20         30         40         50         60 
MQLKPMEINP EMLNKVLTRL GVAGQWRFED VLGLEEESLG SVPAPACALL LLFPLTAQRC 

        70         80         90        100        110        120 
FKLGREAASR FHHPDYPGRL FILLVSQHEN FRKKQIEELK GQEVSPKVYF MKQTIGNSCG 

       130        140        150        160        170        180 
TIGLIHAVAN NQDKLEFEDG SVLKQFLSET EKLSPEDRAK CFEKNEAIQA AHDAVAQEGQ 

       190        200        210        220        230        240 
CRVDDKVNFH FILFNNVDGH LYELDGRMPF PVNHGTSSED SLLQDAAKVC REFTEREQGE 

       250 
VRFSAVALCK AA 

« Hide

References

« Hide 'large scale' references
[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Crossbred X Angus.
Tissue: Liver.
[2]"'Neuron-specific' protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state."
Giambanco I., Bianchi R., Ceccarelli P., Pula G., Sorci G., Antonioli S., Bocchini V., Donato R.
FEBS Lett. 290:131-134(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-25.
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC111330 mRNA. Translation: AAI11331.1.
PIRS17561.
RefSeqNP_001039637.1. NM_001046172.2.
UniGeneBt.58848.

3D structure databases

ProteinModelPortalP23356.
SMRP23356. Positions 1-252.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000044075.

Protein family/group databases

MEROPSC12.001.

Proteomic databases

PaxDbP23356.
PRIDEP23356.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000046823; ENSBTAP00000044075; ENSBTAG00000005078.
GeneID514394.
KEGGbta:514394.

Organism-specific databases

CTD7345.

Phylogenomic databases

eggNOGNOG327708.
GeneTreeENSGT00510000046640.
HOGENOMHOG000182400.
HOVERGENHBG075483.
InParanoidP23356.
KOK05611.
OMAKSHENGH.
OrthoDBEOG7S7SFK.
TreeFamTF316166.

Family and domain databases

Gene3D3.40.532.10. 1 hit.
InterProIPR001578. Peptidase_C12_UCH.
[Graphical view]
PANTHERPTHR10589. PTHR10589. 1 hit.
PfamPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSPR00707. UBCTHYDRLASE.
PROSITEPS00140. UCH_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20871312.

Entry information

Entry nameUCHL1_BOVIN
AccessionPrimary (citable) accession number: P23356
Secondary accession number(s): Q2NKZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: June 13, 2006
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries