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Protein

Ubiquitin carboxyl-terminal hydrolase isozyme L1

Gene

UCHL1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei119 – 1191NucleophilePROSITE-ProRule annotation
Active sitei190 – 1901Proton donorPROSITE-ProRule annotation
Sitei205 – 2051Important for enzyme activity

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: Ensembl
  2. ligase activity Source: UniProtKB-KW
  3. omega peptidase activity Source: Ensembl
  4. ubiquitin-specific protease activity Source: InterPro

GO - Biological processi

  1. adult walking behavior Source: Ensembl
  2. axon target recognition Source: Ensembl
  3. axon transport of mitochondrion Source: Ensembl
  4. cell proliferation Source: Ensembl
  5. eating behavior Source: Ensembl
  6. muscle fiber development Source: Ensembl
  7. negative regulation of MAP kinase activity Source: Ensembl
  8. neuromuscular process Source: Ensembl
  9. protein deubiquitination Source: Ensembl
  10. response to ischemia Source: Ensembl
  11. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Ligase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC12.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase isozyme L1 (EC:3.4.19.12, EC:6.-.-.-)
Short name:
UCH-L1
Alternative name(s):
Neuron cytoplasmic protein 9.5
PGP 9.5
Short name:
PGP9.5
Ubiquitin thioesterase L1
Gene namesi
Name:UCHL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 6

Subcellular locationi

GO - Cellular componenti

  1. axon Source: Ensembl
  2. cytosol Source: Ensembl
  3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  4. extracellular vesicular exosome Source: Ensembl
  5. neuronal cell body Source: Ensembl
  6. nucleus Source: Ensembl
  7. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Ubiquitin carboxyl-terminal hydrolase isozyme L1PRO_0000211053Add
BLAST
Propeptidei250 – 2523Removed in mature formBy similarityPRO_0000414309

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi249 – 2491S-farnesyl cysteineBy similarity

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Prenylation

Proteomic databases

PaxDbiP23356.
PRIDEiP23356.

Expressioni

Tissue specificityi

Neurons and cells of the diffuse neuroendocrine system and their tumors.

Interactioni

Subunit structurei

Monomer. Homodimer. Interacts with COPS5 and SNCA (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044075.

Structurei

3D structure databases

ProteinModelPortaliP23356.
SMRiP23356. Positions 1-252.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni5 – 106Interaction with ubiquitinBy similarity
Regioni180 – 1856Interaction with ubiquitinBy similarity
Regioni240 – 2456Interaction with ubiquitinBy similarity

Sequence similaritiesi

Belongs to the peptidase C12 family.Curated

Phylogenomic databases

eggNOGiNOG327708.
GeneTreeiENSGT00510000046640.
HOGENOMiHOG000182400.
HOVERGENiHBG075483.
InParanoidiP23356.
KOiK05611.
OMAiGQWRFED.
OrthoDBiEOG7S7SFK.
TreeFamiTF316166.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR030297. UCHL1.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PTHR10589:SF19. PTHR10589:SF19. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23356-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQLKPMEINP EMLNKVLTRL GVAGQWRFED VLGLEEESLG SVPAPACALL
60 70 80 90 100
LLFPLTAQRC FKLGREAASR FHHPDYPGRL FILLVSQHEN FRKKQIEELK
110 120 130 140 150
GQEVSPKVYF MKQTIGNSCG TIGLIHAVAN NQDKLEFEDG SVLKQFLSET
160 170 180 190 200
EKLSPEDRAK CFEKNEAIQA AHDAVAQEGQ CRVDDKVNFH FILFNNVDGH
210 220 230 240 250
LYELDGRMPF PVNHGTSSED SLLQDAAKVC REFTEREQGE VRFSAVALCK

AA
Length:252
Mass (Da):28,335
Last modified:June 13, 2006 - v2
Checksum:i9D62A458590188B7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti9 – 91N → D AA sequence (PubMed:1833240).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111330 mRNA. Translation: AAI11331.1.
PIRiS17561.
RefSeqiNP_001039637.1. NM_001046172.2.
UniGeneiBt.58848.

Genome annotation databases

EnsembliENSBTAT00000046823; ENSBTAP00000044075; ENSBTAG00000005078.
GeneIDi514394.
KEGGibta:514394.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC111330 mRNA. Translation: AAI11331.1.
PIRiS17561.
RefSeqiNP_001039637.1. NM_001046172.2.
UniGeneiBt.58848.

3D structure databases

ProteinModelPortaliP23356.
SMRiP23356. Positions 1-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044075.

Protein family/group databases

MEROPSiC12.001.

Proteomic databases

PaxDbiP23356.
PRIDEiP23356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000046823; ENSBTAP00000044075; ENSBTAG00000005078.
GeneIDi514394.
KEGGibta:514394.

Organism-specific databases

CTDi7345.

Phylogenomic databases

eggNOGiNOG327708.
GeneTreeiENSGT00510000046640.
HOGENOMiHOG000182400.
HOVERGENiHBG075483.
InParanoidiP23356.
KOiK05611.
OMAiGQWRFED.
OrthoDBiEOG7S7SFK.
TreeFamiTF316166.

Miscellaneous databases

NextBioi20871312.

Family and domain databases

Gene3Di3.40.532.10. 1 hit.
InterProiIPR001578. Peptidase_C12_UCH.
IPR030297. UCHL1.
[Graphical view]
PANTHERiPTHR10589. PTHR10589. 1 hit.
PTHR10589:SF19. PTHR10589:SF19. 1 hit.
PfamiPF01088. Peptidase_C12. 1 hit.
[Graphical view]
PRINTSiPR00707. UBCTHYDRLASE.
PROSITEiPS00140. UCH_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Crossbred X Angus.
    Tissue: Liver.
  2. "'Neuron-specific' protein gene product 9.5 (PGP 9.5) is also expressed in glioma cell lines and its expression depends on cellular growth state."
    Giambanco I., Bianchi R., Ceccarelli P., Pula G., Sorci G., Antonioli S., Bocchini V., Donato R.
    FEBS Lett. 290:131-134(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-25.
    Tissue: Brain.

Entry informationi

Entry nameiUCHL1_BOVIN
AccessioniPrimary (citable) accession number: P23356
Secondary accession number(s): Q2NKZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: June 13, 2006
Last modified: March 4, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to UCHL3, does not hydrolyze a peptide bond at the C-terminal glycine of NEDD8.By similarity

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.