Reviewed, UniProtKB/Swiss-Prot P23355 (PTFBC_XANCP)

Names and origin

Protein names

Recommended name:
    PTS system fructose-specific EIIBC component
Alternative name(s):
    EIIBC-Fru
      Short name=EII-Fru
Including the following 2 domains:
    1- Recommended name:
            Fructose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system fructose-specific EIIB component
    2- Recommended name:
            Fructose permease IIC component
        Alternative name(s):
            PTS system fructose-specific EIIC component

Gene names

Name:	fruA
Ordered Locus Names:	XCC2372

Organism

Xanthomonas campestris pv. campestris [Complete proteome] [HAMAP]

Taxonomic identifier

340 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Domain	The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
Sequence similarities	Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 580	580	PTS system fructose-specific EIIBC component		PRO_0000186515
Regions
	Transmembrane	254 – 274	21	Potential
	Transmembrane	289 – 309	21	Potential
	Transmembrane	332 – 352	21	Potential
	Transmembrane	369 – 389	21	Potential
	Transmembrane	410 – 430	21	Potential
	Transmembrane	451 – 471	21	Potential
	Transmembrane	483 – 503	21	Potential
	Transmembrane	509 – 529	21	Potential
	Transmembrane	549 – 571	23	Potential
	Domain	120 – 215	96	PTS EIIB type-2
	Domain	243 – 580	338	PTS EIIC type-2
Sites
	Active site	126	1	Phosphocysteine intermediate; for EIIB activity By similarity
Experimental info
	Sequence conflict	150	1	Q → H in AAA27602. Ref.1
	Sequence conflict	260 – 264	5	AGGLL → LAACW in AAA27602. Ref.1
	Sequence conflict	350	1	V → L in AAA27602. Ref.1
	Sequence conflict	408 – 409	2	GS → AG in AAA27602. Ref.1
	Sequence conflict	494 – 495	2	VT → DS in AAA27602. Ref.1
	Sequence conflict	568	1	L → V in AAA27602. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23355-1 [UniParc]. Last modified July 11, 2002. Version 2. Checksum: E6A3B52A7F45662B	FASTA	580	58,647
	10 20 30 40 50 60 MSSSIVVIAA GERSTEAVLA AEALRRAATA AGRSVTIEIR SDQGVLGALP TELTNGAAHV 70 80 90 100 110 120 LIVGDADADT ARFGDAQLLH LSLGAVLDDP AAAVSQLAAT TAPASTSATT DASGAGGKRI 130 140 150 160 170 180 VAITSCPTGI AHTFMAAEGL QQAAKKLGYQ MRVETQGSVG AQDALTDEEI RAADVVIIAA 190 200 210 220 230 240 DREVDLARFG GKRLFKSGTK PAINDGPALI QKALAEAGVH GGAAPVAGAN ATSDAKGNAR 250 260 270 280 290 300 TGAYKHLMTG VSFMLPFVTA GGLLIALAFA LGGIYAGDDA HQGTLAWSLF QIGAKAGFTL 310 320 330 340 350 360 MVPALAGYIA YSIADRPGIA PGMIGGLVAA NLNAGFLGGI IAGFIAGYGV AALNRYIKLP 370 380 390 400 410 420 RNLEGLKPVL ILPVLGTLLV GLAMMYVFGQ PVADLLAWLT AWLRGMQGSS ALLLGLLLGG 430 440 450 460 470 480 MMAFDMGGPV NKAAYAFSTG LIASQVYTPM AAAMVAGMTP PLGIALATWV FRNRFTVEER 490 500 510 520 530 540 GSATAAGVLG LAFVTEGAIP YAARDPLRTI PALVIGSAVA GAISMTAGAE LKAPHGGIFV 550 560 570 580 LLIPNAVTHL LNYVLALVVG VVVTAVALRL LKKPVADVIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fructose catabolism in Xanthomonas campestris pv. campestris. Sequence of the PTS operon, characterization of the fructose-specific enzymes." de Crecy-Lagard V., Bouvet O.M., Lejeune P., Danchin A. J. Biol. Chem. 266:18154-18161(1991) [PubMed: 1655739] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13951 / NCIB 11803 / NRRL B-1459.
[2]	"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed: 12024217] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33913 / NCPPB 528 / LMG 568.
[3]	"Identification of two fructose transport and phosphorylation pathways in Xanthomonas campestris pv. campestris." de Crecy-Lagard V., Lejeune P., Bouvet O.M., Danchin A. Mol. Gen. Genet. 227:465-472(1991) [PubMed: 1650911] [Abstract] Cited for: PROTEIN SEQUENCE OF 404-547.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M69242 Genomic DNA. Translation: AAA27602.1. AE012346 Genomic DNA. Translation: AAM41650.1.
PIR	B40944.
RefSeq	NP_637726.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	998895.
GenomeReviews	Gene locus XCC2372 in contig AE008922_GR.
KEGG	xcc:XCC2372.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P23355.
OMA	P23355. TGLVMIY.
Enzyme and pathway databases
BioCyc	XCAM190485:XCC2372-MON.
BRENDA	2.7.1.69. 281360.
Family and domain databases
InterPro	IPR013011. PTS_EIIB_2. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR003353. PTS_IIB_fruc. IPR006327. PTS_IIC_fruc. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02379. PTS_IIB_fruc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00829. FRU. 1 hit. TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITE	PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFBC_XANCP

Accession

Primary (citable) accession number: P23355

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	July 11, 2002
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)