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Reviewed, UniProtKB/Swiss-Prot P23342 (MSOX_BACSN)

Last modified June 16, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Monomeric sarcosine oxidase
      Short name=MSOX
    EC=1.5.3.1
Gene names
Name: soxA
OrganismBacillus sp. (strain NS-129)
Taxonomic identifier1419 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length387 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the oxidative demethylation of sarcosine By similarity.

Catalytic activity

Sarcosine + H2O + O2 = glycine + formaldehyde + H2O2. HAMAP MF_00516

Cofactor

Binds 1 FAD per subunit. HAMAP MF_00516

Subunit structure

Monomer. HAMAP MF_00516

Subcellular location

Cytoplasm. HAMAP MF_00516

Induction

By sarcosine. HAMAP MF_00516

Sequence similarities

Belongs to the MSOX/MTOX family. MSOX subfamily.

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Ontologies

Keywords
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processoxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tetrahydrofolate metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionsarcosine oxidase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 387387Monomeric sarcosine oxidase HAMAP MF_00516
PRO_0000213761

Regions

Nucleotide binding6 – 3631FAD Potential

Amino acid modifications

Modified residue3161S-8alpha-FAD cysteine By similarity

Secondary structure

1 ...................................................................... 387
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P23342-1 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 7A960CC422841F98

FASTA38742,873
        10         20         30         40         50         60 
MSTHFDVIVV GAGSMGMAAG YYLAKQGVKT LLVDSFDPPH TNGSHHGDTR IIRHAYGEGR 

        70         80         90        100        110        120 
EYVPFALRAQ ELWYELEKET HHKIFTQTGV LVYGPKGGSA FVSETMEAAN IHSLEHELFE 

       130        140        150        160        170        180 
GKQLTDRWAG VEVPDNYEAI FEPNSGVLFS ENCIQAYREL AEAHGATVLT YTPVEDFEVT 

       190        200        210        220        230        240 
EDLVTIKTAK GSYTANKLVV SMGAWNSKLL SKLDVEIPLQ PYRQVVGFFE CDEAKYSNNA 

       250        260        270        280        290        300 
HYPAFMVEVE NGIYYGFPSF GGSGLKIGYH SYGQQIDPDT INREFGAYPE DEANLRKFLE 

       310        320        330        340        350        360 
QYMPGANGEL KKGAVCMYTK TPDEHFVIDL HPKYSNVAIA AGFSGHGFKF SSVVGETLAQ 

       370        380 
LATTGKTEHD ISIFSLNRDA LKKEAVK

« Hide

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References

[1]"Cloning and expression of the sarcosine oxidase gene from Bacillus sp. NS-129 in Escherichia coli."
Koyama Y., Yamamoto-Otake H., Suzuki M., Nakano E.
Agric. Biol. Chem. 55:1259-1263(1991) [PubMed: 1368683] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-34.

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Cross-references

Sequence databases

D10553 Genomic DNA. Translation: BAA01410.1.
PIRJU0461.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1ZOVX-ray1.86A/B2-387[»]
ModBaseSearch...

Family and domain databases

HAMAPMF_00516.
[Tree]
InterProIPR006076. FAD-dep_OxRdtase.
IPR006281. SoxA_mon.
[Graphical view]
PfamPF01266. DAO. 1 hit.
[Graphical view]
TIGRFAMsTIGR01377. soxA_mon. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameMSOX_BACSN
AccessionPrimary (citable) accession number: P23342
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents