Reviewed, UniProtKB/Swiss-Prot P23339 (RIPS_PHYAM)

Names and origin

Protein names	Recommended name:     Antiviral protein S     EC=3.2.2.22 Alternative name(s):     PAP-S     Ribosome-inactivating protein     rRNA N-glycosidase
Organism	Phytolacca americana (American pokeweed) (Phytolacca decandra)
Taxonomic identifier	3527 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Phytolaccaceae › Phytolacca

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits viral infection of plants, and protein synthesis in vitro.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Antiviral defense Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW response to virus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 261

261

Antiviral protein S

PRO_0000221415

Sites

Active site

175

1

By similarity

Amino acid modifications

Disulfide bond

34 ↔ 258

Ref.1

Disulfide bond

84 ↔ 105

Ref.1

Secondary structure

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261

Helix Strand Turn

Details...

Beta strand

3 – 10

8

Helix

13 – 27

15

Beta strand

34 – 36

3

Beta strand

49 – 55

7

Beta strand

61 – 67

7

Turn

68 – 70

3

Beta strand

73 – 80

8

Beta strand

83 – 88

6

Helix

95 – 104

10

Beta strand

111 – 113

3

Helix

122 – 129

8

Helix

134 – 136

3

Helix

141 – 151

11

Helix

159 – 172

14

Helix

174 – 178

5

Helix

180 – 188

9

Beta strand

190 – 192

3

Helix

198 – 205

8

Helix

207 – 215

9

Helix

218 – 220

3

Beta strand

221 – 229

9

Beta strand

235 – 240

6

Helix

241 – 244

4

Sequences

	Sequence		Length	Mass (Da)
	P23339-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: D88B99962FE8399D	FASTA	261	29,200
	10 20 30 40 50 60 INTITFDAGN ATINKYATFM ESLRNEAKDP SLKCYGIPML PNTNSTIKYL LVKLQGASLK 70 80 90 100 110 120 TITLMLRRNN LYVMGYSDPY DNKCRYHIFN DIKGTEYSDV ENTLCPSSNP RVAKPINYNG 130 140 150 160 170 180 LYPTLEKKAG VTSRNEVQLG IQILSSDIGK ISGQGSFTEK IEAKFLLVAI QMVSEAARFK 190 200 210 220 230 240 YIENQVKTNF NRDFSPNDKV LDLEENWGKI STAIHNSKNG ALPKPLELKN ADGTKWIVLR 250 260 VDEIKPDVGL LNYVNGTCQA T

References

[1]	"The complete amino acid sequence of antiviral protein from the seeds of pokeweed (Phytolacca americana)." Kung S.S., Kimura M., Funatsu G. Agric. Biol. Chem. 54:3301-3318(1990) [PubMed: 1368643] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Seed.
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

JE0401.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GIK	X-ray	1.80	A	1-261	[»]
1J1Q	X-ray	1.80	A	1-261	[»]
1J1R	X-ray	1.90	A	1-261	[»]
1J1S	X-ray	2.00	A	1-261	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

3.2.2.22. 257893.

Family and domain databases

InterPro

IPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]

Gene3D

G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.

Pfam

PF00161. RIP. 1 hit.
[Graphical view]

PRINTS

PR00396. SHIGARICIN.

PROSITE

PS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB00173. Adenine.

Entry information

Entry name

RIPS_PHYAM

Accession

Primary (citable) accession number: P23339

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)