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P23338

- GPT_CRILO

UniProt

P23338 - GPT_CRILO

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Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene
DPAGT1, DPAGT2, GNPTA
Organism
Cricetulus longicaudatus (Long-tailed dwarf hamster) (Chinese hamster)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

Enzyme regulationi

Enzyme activity is stimulated by phosphatidylglycerol and mannosylphosphoryldolichol and inhibited by tunicamycin.

Pathwayi

GO - Molecular functioni

  1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
  2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
  3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
Alternative name(s):
GlcNAc-1-P transferase
Short name:
G1PT
Short name:
GPT
N-acetylglucosamine-1-phosphate transferase
Gene namesi
Name:DPAGT1
Synonyms:DPAGT2, GNPTA
OrganismiCricetulus longicaudatus (Long-tailed dwarf hamster) (Chinese hamster)
Taxonomic identifieri10030 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66Lumenal Reviewed prediction
Transmembranei7 – 3226Helical; Reviewed predictionAdd
BLAST
Topological domaini33 – 5725Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei58 – 7922Helical; Reviewed predictionAdd
BLAST
Topological domaini80 – 9415Lumenal Reviewed predictionAdd
BLAST
Transmembranei95 – 11420Helical; Reviewed predictionAdd
BLAST
Topological domaini115 – 12511Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei126 – 14520Helical; Reviewed predictionAdd
BLAST
Topological domaini146 – 16419Lumenal Reviewed predictionAdd
BLAST
Transmembranei165 – 18420Helical; Reviewed predictionAdd
BLAST
Topological domaini185 – 19410Cytoplasmic Reviewed prediction
Transmembranei195 – 21117Helical; Reviewed predictionAdd
BLAST
Topological domaini212 – 22110Lumenal Reviewed prediction
Transmembranei222 – 24019Helical; Reviewed predictionAdd
BLAST
Topological domaini241 – 25212Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei253 – 26917Helical; Reviewed predictionAdd
BLAST
Topological domaini270 – 2745Lumenal Reviewed prediction
Transmembranei275 – 29420Helical; Reviewed predictionAdd
BLAST
Topological domaini295 – 37884Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei379 – 39719Helical; Reviewed predictionAdd
BLAST
Topological domaini398 – 40811Lumenal Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108759Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi146 – 1461N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi67 – 7913Dolichol recognitionAdd
BLAST
Motifi222 – 23413Dolichol recognitionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG000846.

Family and domain databases

InterProiIPR000715. Glycosyl_transferase_4.
[Graphical view]
PfamiPF00953. Glycos_transf_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23338-1 [UniParc]FASTAAdd to Basket

« Hide

MWAFPELPLP LLVNLFGSLL GFVATVTLIP AFRSHFIAAR LCGQDLNKLS    50
RQQIPESQGV ICGAVFLIIL FCFIPFPFLN CFVEEQCKAF PHHEFVALIG 100
ALLAICCMIF LGFADDVLNL PWRHKLLLPT AASLPLLMVY FTNFGNTTIV 150
VPKPFRWILG LHLDLGILYY VYMGLLAVFC TNAINILAGI NGLEAGQSLV 200
ISASIIVFNL VELEGDYRDD HVFSLYFMIP FFFTTLGLLY HNWYPSQVFV 250
GDTFCYFAGM TFAVVGILGH FSKTMLLFFI PQVFNFLYSL PQLLHAIPCP 300
RHRIPRLNPK TGKLEMSYSK FKTKNLSFLG TFILKVAERL QLVTVHRGES 350
EDGAFTECNN MTLINLLLKI FGPIHERNLT LLLLLLQILS SAVTFSIRYQ 400
LVRLFYDV 408
Length:408
Mass (Da):46,191
Last modified:November 1, 1991 - v1
Checksum:i108C4D7598B8F4F3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05590 mRNA. Translation: AAA36965.1.
PIRiA37813.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J05590 mRNA. Translation: AAA36965.1 .
PIRi A37813.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG000846.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

InterProi IPR000715. Glycosyl_transferase_4.
[Graphical view ]
Pfami PF00953. Glycos_transf_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequence, and expression of a cDNA encoding hamster UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1-phosphate transferase."
    Zhu X., Lehrman M.A.
    J. Biol. Chem. 265:14250-14255(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Amplification and molecular cloning of the hamster tunicamycin-sensitive N-acetylglucosamine-1-phosphate transferase gene. The hamster and yeast enzymes share a common peptide sequence."
    Lehrman M.A., Zhu X., Khounlo S.
    J. Biol. Chem. 263:19796-19803(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 248-271.

Entry informationi

Entry nameiGPT_CRILO
AccessioniPrimary (citable) accession number: P23338
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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