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P23338

- GPT_CRILO

UniProt

P23338 - GPT_CRILO

Protein

UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase

Gene

DPAGT1

Organism
Cricetulus longicaudatus (Long-tailed dwarf hamster) (Chinese hamster)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Catalyzes the initial step in the synthesis of dolichol-P-P-oligosaccharides.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + dolichyl phosphate = UMP + N-acetyl-D-glucosaminyl-diphosphodolichol.

    Enzyme regulationi

    Enzyme activity is stimulated by phosphatidylglycerol and mannosylphosphoryldolichol and inhibited by tunicamycin.

    Pathwayi

    GO - Molecular functioni

    1. phospho-N-acetylmuramoyl-pentapeptide-transferase activity Source: InterPro
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW
    3. UDP-N-acetylglucosamine-dolichyl-phosphate N-acetylglucosaminephosphotransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (EC:2.7.8.15)
    Alternative name(s):
    GlcNAc-1-P transferase
    Short name:
    G1PT
    Short name:
    GPT
    N-acetylglucosamine-1-phosphate transferase
    Gene namesi
    Name:DPAGT1
    Synonyms:DPAGT2, GNPTA
    OrganismiCricetulus longicaudatus (Long-tailed dwarf hamster) (Chinese hamster)
    Taxonomic identifieri10030 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 408408UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferasePRO_0000108759Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66LumenalSequence Analysis
    Topological domaini33 – 5725CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini80 – 9415LumenalSequence AnalysisAdd
    BLAST
    Topological domaini115 – 12511CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini146 – 16419LumenalSequence AnalysisAdd
    BLAST
    Topological domaini185 – 19410CytoplasmicSequence Analysis
    Topological domaini212 – 22110LumenalSequence Analysis
    Topological domaini241 – 25212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini270 – 2745LumenalSequence Analysis
    Topological domaini295 – 37884CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini398 – 40811LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 3226HelicalSequence AnalysisAdd
    BLAST
    Transmembranei58 – 7922HelicalSequence AnalysisAdd
    BLAST
    Transmembranei95 – 11420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei126 – 14520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei165 – 18420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei195 – 21117HelicalSequence AnalysisAdd
    BLAST
    Transmembranei222 – 24019HelicalSequence AnalysisAdd
    BLAST
    Transmembranei253 – 26917HelicalSequence AnalysisAdd
    BLAST
    Transmembranei275 – 29420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei379 – 39719HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi67 – 7913Dolichol recognitionAdd
    BLAST
    Motifi222 – 23413Dolichol recognitionAdd
    BLAST

    Sequence similaritiesi

    Belongs to the glycosyltransferase 4 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOVERGENiHBG000846.

    Family and domain databases

    InterProiIPR000715. Glycosyl_transferase_4.
    [Graphical view]
    PfamiPF00953. Glycos_transf_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23338-1 [UniParc]FASTAAdd to Basket

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    MWAFPELPLP LLVNLFGSLL GFVATVTLIP AFRSHFIAAR LCGQDLNKLS    50
    RQQIPESQGV ICGAVFLIIL FCFIPFPFLN CFVEEQCKAF PHHEFVALIG 100
    ALLAICCMIF LGFADDVLNL PWRHKLLLPT AASLPLLMVY FTNFGNTTIV 150
    VPKPFRWILG LHLDLGILYY VYMGLLAVFC TNAINILAGI NGLEAGQSLV 200
    ISASIIVFNL VELEGDYRDD HVFSLYFMIP FFFTTLGLLY HNWYPSQVFV 250
    GDTFCYFAGM TFAVVGILGH FSKTMLLFFI PQVFNFLYSL PQLLHAIPCP 300
    RHRIPRLNPK TGKLEMSYSK FKTKNLSFLG TFILKVAERL QLVTVHRGES 350
    EDGAFTECNN MTLINLLLKI FGPIHERNLT LLLLLLQILS SAVTFSIRYQ 400
    LVRLFYDV 408
    Length:408
    Mass (Da):46,191
    Last modified:November 1, 1991 - v1
    Checksum:i108C4D7598B8F4F3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05590 mRNA. Translation: AAA36965.1.
    PIRiA37813.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05590 mRNA. Translation: AAA36965.1 .
    PIRi A37813.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG000846.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    InterProi IPR000715. Glycosyl_transferase_4.
    [Graphical view ]
    Pfami PF00953. Glycos_transf_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequence, and expression of a cDNA encoding hamster UDP-GlcNAc:dolichol phosphate N-acetylglucosamine-1-phosphate transferase."
      Zhu X., Lehrman M.A.
      J. Biol. Chem. 265:14250-14255(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Amplification and molecular cloning of the hamster tunicamycin-sensitive N-acetylglucosamine-1-phosphate transferase gene. The hamster and yeast enzymes share a common peptide sequence."
      Lehrman M.A., Zhu X., Khounlo S.
      J. Biol. Chem. 263:19796-19803(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 248-271.

    Entry informationi

    Entry nameiGPT_CRILO
    AccessioniPrimary (citable) accession number: P23338
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3