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P23337

- GYS1_YEAST

UniProt

P23337 - GYS1_YEAST

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Protein

Glycogen [starch] synthase isoform 1

Gene
GSY1, YFR015C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.

Catalytic activityi

UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

Enzyme regulationi

Allosteric activation by glucose-6-phosphate, and phosphorylation by a cAMP-dependent kinase.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201UDP-glucose By similarity

GO - Molecular functioni

  1. glycogen (starch) synthase activity Source: SGD

GO - Biological processi

  1. glycogen biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YFR015C-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen [starch] synthase isoform 1 (EC:2.4.1.11)
Gene namesi
Name:GSY1
Ordered Locus Names:YFR015C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VI

Organism-specific databases

SGDiS000001911. GSY1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 708707Glycogen [starch] synthase isoform 1PRO_0000194773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei159 – 1591Phosphoserine Reviewed prediction
Modified residuei363 – 3631Phosphoserine Reviewed prediction
Modified residuei560 – 5601Phosphoserine Reviewed prediction
Modified residuei651 – 6511Phosphoserine4 Publications
Modified residuei655 – 6551Phosphoserine5 Publications
Modified residuei660 – 6601Phosphoserine; by PKA Reviewed prediction
Modified residuei662 – 6621Phosphoserine; by PKA Reviewed prediction

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23337.
PaxDbiP23337.
PeptideAtlasiP23337.

Expressioni

Developmental stagei

Activity increases just before cells entry in the stationary phase.

Inductioni

Synthesized in response to growth limitation.

Gene expression databases

GenevestigatoriP23337.

Interactioni

Protein-protein interaction databases

BioGridi31167. 32 interactions.
DIPiDIP-5354N.
IntActiP23337. 12 interactions.
MINTiMINT-483654.
STRINGi4932.YFR015C.

Structurei

3D structure databases

ProteinModelPortaliP23337.
SMRiP23337. Positions 2-639.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
KOiK00693.
OMAiVSWEVAN.
OrthoDBiEOG7QK0MF.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23337-1 [UniParc]FASTAAdd to Basket

« Hide

MARDLQNHLL FEVATEVTNR VGGIYSVLKS KAPVTVAQYG DNYTLLGPLN    50
KATYESEVEK LDWEDESIFP EELLPIQKTL MSMREKGVNF VYGNWLIEGA 100
PRVILFELDS VRHFLNEWKA DLWSLVGIPS PEHDHETNDA ILLGYVVVWF 150
LGEVSKLDSS HAIIGHFHEW LAGVALPLCR KKRIDVVTIF TTHATLLGRY 200
LCAAGDVDFY NNLQYFDVDQ EAGKRGIYHR YCIERAAAHT ADVFTTVSQI 250
TALEAEHLLK RKPDGILPNG LNVVKFQAVH EFQNLHALKK DKINDFVRGH 300
FHGCFDFDLD NTVYFFIAGR YEYKNKGADM FIESLARLNY RLKVSGSKKT 350
VVAFLIMPAK TNSFTVEALK SQAIVKSLEN TVNEVTASIG KRIFEHTMRY 400
PHNGLESELP TNLDELLKSS EKVLLKKRVL ALRRPYGELP PVVTHNMCDD 450
ANDPILNQIR HVRLFNDSSD RVKVIFHPEF LNANNPILGL DYDEFVRGCH 500
LGVFPSYYEP WGYTPAECTV MGVPSITTNV SGFGAYMEDL IETDQAKDYG 550
IYIVDRRFKS PDESVEQLAD YMEEFVNKTR RQRINQRNRT ERLSDLLDWK 600
RMGLEYVKAR QLGLRRAYPE QFKQLVGETI SDANMNTLAG GKKFKIARPL 650
SVPGSPKVRS NSTVYMTPGD LGTLQDANNA DDYFNLSTNG AIDNDDDDND 700
TSAYYEDN 708
Length:708
Mass (Da):80,510
Last modified:January 23, 2007 - v3
Checksum:i9EF2D3858529E68A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60919 Genomic DNA. Translation: AAA88715.1.
D50617 Genomic DNA. Translation: BAA09254.1.
BK006940 Genomic DNA. Translation: DAA12456.1.
PIRiA38326.
RefSeqiNP_116670.1. NM_001179980.1.

Genome annotation databases

EnsemblFungiiYFR015C; YFR015C; YFR015C.
GeneIDi850569.
KEGGisce:YFR015C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60919 Genomic DNA. Translation: AAA88715.1 .
D50617 Genomic DNA. Translation: BAA09254.1 .
BK006940 Genomic DNA. Translation: DAA12456.1 .
PIRi A38326.
RefSeqi NP_116670.1. NM_001179980.1.

3D structure databases

ProteinModelPortali P23337.
SMRi P23337. Positions 2-639.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31167. 32 interactions.
DIPi DIP-5354N.
IntActi P23337. 12 interactions.
MINTi MINT-483654.
STRINGi 4932.YFR015C.

Protein family/group databases

CAZyi GT3. Glycosyltransferase Family 3.

Proteomic databases

MaxQBi P23337.
PaxDbi P23337.
PeptideAtlasi P23337.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YFR015C ; YFR015C ; YFR015C .
GeneIDi 850569.
KEGGi sce:YFR015C.

Organism-specific databases

SGDi S000001911. GSY1.

Phylogenomic databases

eggNOGi COG0438.
GeneTreei ENSGT00390000018612.
HOGENOMi HOG000160890.
KOi K00693.
OMAi VSWEVAN.
OrthoDBi EOG7QK0MF.

Enzyme and pathway databases

UniPathwayi UPA00164 .
BioCyci YEAST:YFR015C-MONOMER.

Miscellaneous databases

NextBioi 966381.

Gene expression databases

Genevestigatori P23337.

Family and domain databases

InterProi IPR008631. Glycogen_synth.
[Graphical view ]
PANTHERi PTHR10176. PTHR10176. 1 hit.
Pfami PF05693. Glycogen_syn. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of the GSY1 gene encoding yeast glycogen synthase and evidence for the existence of a second gene."
    Farkas I., Hardy T.A., Depaoli-Roach A.A., Roach P.J.
    J. Biol. Chem. 265:20879-20886(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 2-17; 52-62; 86-105; 377-391; 593-600; 602-608 AND 660-669, CLEAVAGE OF INITIATOR METHIONINE.
    Strain: YPH52.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGYS1_YEAST
AccessioniPrimary (citable) accession number: P23337
Secondary accession number(s): D6VTP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 137 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6300 molecules/cell in log phase SD medium.

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

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