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Reviewed, UniProtKB/Swiss-Prot P23337 (GYS1_YEAST)

Last modified June 16, 2009. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glycogen [starch] synthase isoform 1
    EC=2.4.1.11
Gene names
Name: GSY1
Ordered Locus Names: YFR015C
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

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Protein attributes

Sequence length708 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.

Catalytic activity

UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

Enzyme regulation

Allosteric activation by glucose-6-phosphate, and phosphorylation by a cAMP-dependent kinase.

Pathway

Glycan biosynthesis; glycogen biosynthesis.

Developmental stage

Activity increases just before cells entry in the stationary phase.

Induction

Synthesized in response to growth limitation.

Miscellaneous

Present with 6300 molecules/cell in log phase SD medium. Ref.4

Sequence similarities

Belongs to the glycosyltransferase 3 family.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-8031,EBI-8031
GLG2P470111EBI-8031,EBI-7646
GSY2P274721EBI-8031,EBI-8036

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 708707Glycogen [starch] synthase isoform 1
PRO_0000194773

Sites

Binding site201UDP-glucose By similarity

Amino acid modifications

Modified residue1591Phosphoserine Potential
Modified residue3631Phosphoserine Potential
Modified residue5601Phosphoserine Potential
Modified residue6511Phosphoserine Ref.3 Ref.5 Ref.6 Ref.7 Ref.8
Modified residue6551Phosphoserine Ref.3 Ref.5 Ref.6 Ref.7 Ref.8
Modified residue6601Phosphoserine; by PKA Potential
Modified residue6621Phosphoserine; by PKA Potential

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Sequences

Sequence LengthMass (Da)Tools
P23337-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9EF2D3858529E68A

FASTA70880,510
        10         20         30         40         50         60 
MARDLQNHLL FEVATEVTNR VGGIYSVLKS KAPVTVAQYG DNYTLLGPLN KATYESEVEK 

        70         80         90        100        110        120 
LDWEDESIFP EELLPIQKTL MSMREKGVNF VYGNWLIEGA PRVILFELDS VRHFLNEWKA 

       130        140        150        160        170        180 
DLWSLVGIPS PEHDHETNDA ILLGYVVVWF LGEVSKLDSS HAIIGHFHEW LAGVALPLCR 

       190        200        210        220        230        240 
KKRIDVVTIF TTHATLLGRY LCAAGDVDFY NNLQYFDVDQ EAGKRGIYHR YCIERAAAHT 

       250        260        270        280        290        300 
ADVFTTVSQI TALEAEHLLK RKPDGILPNG LNVVKFQAVH EFQNLHALKK DKINDFVRGH 

       310        320        330        340        350        360 
FHGCFDFDLD NTVYFFIAGR YEYKNKGADM FIESLARLNY RLKVSGSKKT VVAFLIMPAK 

       370        380        390        400        410        420 
TNSFTVEALK SQAIVKSLEN TVNEVTASIG KRIFEHTMRY PHNGLESELP TNLDELLKSS 

       430        440        450        460        470        480 
EKVLLKKRVL ALRRPYGELP PVVTHNMCDD ANDPILNQIR HVRLFNDSSD RVKVIFHPEF 

       490        500        510        520        530        540 
LNANNPILGL DYDEFVRGCH LGVFPSYYEP WGYTPAECTV MGVPSITTNV SGFGAYMEDL 

       550        560        570        580        590        600 
IETDQAKDYG IYIVDRRFKS PDESVEQLAD YMEEFVNKTR RQRINQRNRT ERLSDLLDWK 

       610        620        630        640        650        660 
RMGLEYVKAR QLGLRRAYPE QFKQLVGETI SDANMNTLAG GKKFKIARPL SVPGSPKVRS 

       670        680        690        700 
NSTVYMTPGD LGTLQDANNA DDYFNLSTNG AIDNDDDDND TSAYYEDN

« Hide

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References

« Hide 'large scale' references
[1]"Isolation of the GSY1 gene encoding yeast glycogen synthase and evidence for the existence of a second gene."
Farkas I., Hardy T.A., Depaoli-Roach A.A., Roach P.J.
J. Biol. Chem. 265:20879-20886(1990) [PubMed: 2123485] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
Strain: YPH52.
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Phosphoproteome analysis by mass spectrometry and its application to Saccharomyces cerevisiae."
Ficarro S.B., McCleland M.L., Stukenberg P.T., Burke D.J., Ross M.M., Shabanowitz J., Hunt D.F., White F.M.
Nat. Biotechnol. 20:301-305(2002) [PubMed: 11875433] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, MASS SPECTROMETRY.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, MASS SPECTROMETRY.
[6]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, MASS SPECTROMETRY.
[7]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, MASS SPECTROMETRY.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, MASS SPECTROMETRY.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M60919 Genomic DNA. Translation: AAA88715.1.
D50617 Genomic DNA. Translation: BAA09254.1.
PIRA38326.
RefSeqNP_116670.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:5354N.
IntActP23337. 10 interactions.

Protein family/group databases

CAZyGT3. Glycosyltransferase Family 3.

Proteomic databases

PeptideAtlasP23337.
PRIDEP23337.

Genome annotation databases

EnsemblYFR015C. Saccharomyces cerevisiae. [Contig view]
GeneID850569.
GenomeReviewsGene locus YFR015C in contig D50617_GR.
KEGGsce:YFR015C.
NMPDRfig|4932.3.peg.2304.

Organism-specific databases

CYGDYFR015c.
SGDS000001911. GSY1.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP23337.
OMAP23337. GKQYLVA.

Enzyme and pathway databases

BRENDA2.4.1.11. 250.

Gene expression databases

ArrayExpressP23337.
GermOnlineYFR015C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR008631. Glycogen_synth.
[Graphical view]
PANTHERPTHR10176. Glycogen_synth. 1 hit.
PfamPF05693. Glycogen_syn. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio966381.

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Entry information

Entry nameGYS1_YEAST
AccessionPrimary (citable) accession number: P23337
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 91 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents