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P23337 (GYS1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycogen [starch] synthase isoform 1

EC=2.4.1.11
Gene names
Name:GSY1
Ordered Locus Names:YFR015C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length708 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.

Catalytic activity

UDP-glucose ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

Enzyme regulation

Allosteric activation by glucose-6-phosphate, and phosphorylation by a cAMP-dependent kinase.

Pathway

Glycan biosynthesis; glycogen biosynthesis.

Developmental stage

Activity increases just before cells entry in the stationary phase.

Induction

Synthesized in response to growth limitation.

Miscellaneous

Present with 6300 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the glycosyltransferase 3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 708707Glycogen [starch] synthase isoform 1
PRO_0000194773

Sites

Binding site201UDP-glucose By similarity

Amino acid modifications

Modified residue1591Phosphoserine Potential
Modified residue3631Phosphoserine Potential
Modified residue5601Phosphoserine Potential
Modified residue6511Phosphoserine Ref.6 Ref.7 Ref.8 Ref.9
Modified residue6551Phosphoserine Ref.5 Ref.6 Ref.7 Ref.8 Ref.9
Modified residue6601Phosphoserine; by PKA Potential
Modified residue6621Phosphoserine; by PKA Potential

Sequences

Sequence LengthMass (Da)Tools
P23337 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 9EF2D3858529E68A

FASTA70880,510
        10         20         30         40         50         60 
MARDLQNHLL FEVATEVTNR VGGIYSVLKS KAPVTVAQYG DNYTLLGPLN KATYESEVEK 

        70         80         90        100        110        120 
LDWEDESIFP EELLPIQKTL MSMREKGVNF VYGNWLIEGA PRVILFELDS VRHFLNEWKA 

       130        140        150        160        170        180 
DLWSLVGIPS PEHDHETNDA ILLGYVVVWF LGEVSKLDSS HAIIGHFHEW LAGVALPLCR 

       190        200        210        220        230        240 
KKRIDVVTIF TTHATLLGRY LCAAGDVDFY NNLQYFDVDQ EAGKRGIYHR YCIERAAAHT 

       250        260        270        280        290        300 
ADVFTTVSQI TALEAEHLLK RKPDGILPNG LNVVKFQAVH EFQNLHALKK DKINDFVRGH 

       310        320        330        340        350        360 
FHGCFDFDLD NTVYFFIAGR YEYKNKGADM FIESLARLNY RLKVSGSKKT VVAFLIMPAK 

       370        380        390        400        410        420 
TNSFTVEALK SQAIVKSLEN TVNEVTASIG KRIFEHTMRY PHNGLESELP TNLDELLKSS 

       430        440        450        460        470        480 
EKVLLKKRVL ALRRPYGELP PVVTHNMCDD ANDPILNQIR HVRLFNDSSD RVKVIFHPEF 

       490        500        510        520        530        540 
LNANNPILGL DYDEFVRGCH LGVFPSYYEP WGYTPAECTV MGVPSITTNV SGFGAYMEDL 

       550        560        570        580        590        600 
IETDQAKDYG IYIVDRRFKS PDESVEQLAD YMEEFVNKTR RQRINQRNRT ERLSDLLDWK 

       610        620        630        640        650        660 
RMGLEYVKAR QLGLRRAYPE QFKQLVGETI SDANMNTLAG GKKFKIARPL SVPGSPKVRS 

       670        680        690        700 
NSTVYMTPGD LGTLQDANNA DDYFNLSTNG AIDNDDDDND TSAYYEDN 

« Hide

References

« Hide 'large scale' references
[1]"Isolation of the GSY1 gene encoding yeast glycogen synthase and evidence for the existence of a second gene."
Farkas I., Hardy T.A., Depaoli-Roach A.A., Roach P.J.
J. Biol. Chem. 265:20879-20886(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 2-17; 52-62; 86-105; 377-391; 593-600; 602-608 AND 660-669, CLEAVAGE OF INITIATOR METHIONINE.
Strain: YPH52.
[2]"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae."
Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T.
Nat. Genet. 10:261-268(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[6]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60919 Genomic DNA. Translation: AAA88715.1.
D50617 Genomic DNA. Translation: BAA09254.1.
BK006940 Genomic DNA. Translation: DAA12456.1.
PIRA38326.
RefSeqNP_116670.1. NM_001179980.1.

3D structure databases

ProteinModelPortalP23337.
SMRP23337. Positions 2-647.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31167. 32 interactions.
DIPDIP-5354N.
IntActP23337. 12 interactions.
MINTMINT-483654.
STRING4932.YFR015C.

Protein family/group databases

CAZyGT3. Glycosyltransferase Family 3.

Proteomic databases

PaxDbP23337.
PeptideAtlasP23337.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYFR015C; YFR015C; YFR015C.
GeneID850569.
KEGGsce:YFR015C.

Organism-specific databases

SGDS000001911. GSY1.

Phylogenomic databases

eggNOGCOG0438.
GeneTreeENSGT00390000018612.
HOGENOMHOG000160890.
KOK00693.
OMAHCARTEM.
OrthoDBEOG7QK0MF.

Enzyme and pathway databases

BioCycYEAST:YFR015C-MONOMER.
UniPathwayUPA00164.

Gene expression databases

GenevestigatorP23337.

Family and domain databases

InterProIPR008631. Glycogen_synth.
[Graphical view]
PANTHERPTHR10176. PTHR10176. 1 hit.
PfamPF05693. Glycogen_syn. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio966381.

Entry information

Entry nameGYS1_YEAST
AccessionPrimary (citable) accession number: P23337
Secondary accession number(s): D6VTP6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VI

Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways