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P23337

- GYS1_YEAST

UniProt

P23337 - GYS1_YEAST

Protein

Glycogen [starch] synthase isoform 1

Gene

GSY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.

    Catalytic activityi

    UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

    Enzyme regulationi

    Allosteric activation by glucose-6-phosphate, and phosphorylation by a cAMP-dependent kinase.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201UDP-glucoseBy similarity

    GO - Molecular functioni

    1. glycogen (starch) synthase activity Source: SGD

    GO - Biological processi

    1. glycogen biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YFR015C-MONOMER.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiGT3. Glycosyltransferase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen [starch] synthase isoform 1 (EC:2.4.1.11)
    Gene namesi
    Name:GSY1
    Ordered Locus Names:YFR015C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VI

    Organism-specific databases

    SGDiS000001911. GSY1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 708707Glycogen [starch] synthase isoform 1PRO_0000194773Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei159 – 1591PhosphoserineSequence Analysis
    Modified residuei363 – 3631PhosphoserineSequence Analysis
    Modified residuei560 – 5601PhosphoserineSequence Analysis
    Modified residuei651 – 6511Phosphoserine4 Publications
    Modified residuei655 – 6551Phosphoserine5 Publications
    Modified residuei660 – 6601Phosphoserine; by PKASequence Analysis
    Modified residuei662 – 6621Phosphoserine; by PKASequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP23337.
    PaxDbiP23337.
    PeptideAtlasiP23337.

    Expressioni

    Developmental stagei

    Activity increases just before cells entry in the stationary phase.

    Inductioni

    Synthesized in response to growth limitation.

    Gene expression databases

    GenevestigatoriP23337.

    Interactioni

    Protein-protein interaction databases

    BioGridi31167. 32 interactions.
    DIPiDIP-5354N.
    IntActiP23337. 12 interactions.
    MINTiMINT-483654.
    STRINGi4932.YFR015C.

    Structurei

    3D structure databases

    ProteinModelPortaliP23337.
    SMRiP23337. Positions 2-639.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00390000018612.
    HOGENOMiHOG000160890.
    KOiK00693.
    OMAiVSWEVAN.
    OrthoDBiEOG7QK0MF.

    Family and domain databases

    InterProiIPR008631. Glycogen_synth.
    [Graphical view]
    PANTHERiPTHR10176. PTHR10176. 1 hit.
    PfamiPF05693. Glycogen_syn. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23337-1 [UniParc]FASTAAdd to Basket

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    MARDLQNHLL FEVATEVTNR VGGIYSVLKS KAPVTVAQYG DNYTLLGPLN    50
    KATYESEVEK LDWEDESIFP EELLPIQKTL MSMREKGVNF VYGNWLIEGA 100
    PRVILFELDS VRHFLNEWKA DLWSLVGIPS PEHDHETNDA ILLGYVVVWF 150
    LGEVSKLDSS HAIIGHFHEW LAGVALPLCR KKRIDVVTIF TTHATLLGRY 200
    LCAAGDVDFY NNLQYFDVDQ EAGKRGIYHR YCIERAAAHT ADVFTTVSQI 250
    TALEAEHLLK RKPDGILPNG LNVVKFQAVH EFQNLHALKK DKINDFVRGH 300
    FHGCFDFDLD NTVYFFIAGR YEYKNKGADM FIESLARLNY RLKVSGSKKT 350
    VVAFLIMPAK TNSFTVEALK SQAIVKSLEN TVNEVTASIG KRIFEHTMRY 400
    PHNGLESELP TNLDELLKSS EKVLLKKRVL ALRRPYGELP PVVTHNMCDD 450
    ANDPILNQIR HVRLFNDSSD RVKVIFHPEF LNANNPILGL DYDEFVRGCH 500
    LGVFPSYYEP WGYTPAECTV MGVPSITTNV SGFGAYMEDL IETDQAKDYG 550
    IYIVDRRFKS PDESVEQLAD YMEEFVNKTR RQRINQRNRT ERLSDLLDWK 600
    RMGLEYVKAR QLGLRRAYPE QFKQLVGETI SDANMNTLAG GKKFKIARPL 650
    SVPGSPKVRS NSTVYMTPGD LGTLQDANNA DDYFNLSTNG AIDNDDDDND 700
    TSAYYEDN 708
    Length:708
    Mass (Da):80,510
    Last modified:January 23, 2007 - v3
    Checksum:i9EF2D3858529E68A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60919 Genomic DNA. Translation: AAA88715.1.
    D50617 Genomic DNA. Translation: BAA09254.1.
    BK006940 Genomic DNA. Translation: DAA12456.1.
    PIRiA38326.
    RefSeqiNP_116670.1. NM_001179980.1.

    Genome annotation databases

    EnsemblFungiiYFR015C; YFR015C; YFR015C.
    GeneIDi850569.
    KEGGisce:YFR015C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60919 Genomic DNA. Translation: AAA88715.1 .
    D50617 Genomic DNA. Translation: BAA09254.1 .
    BK006940 Genomic DNA. Translation: DAA12456.1 .
    PIRi A38326.
    RefSeqi NP_116670.1. NM_001179980.1.

    3D structure databases

    ProteinModelPortali P23337.
    SMRi P23337. Positions 2-639.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31167. 32 interactions.
    DIPi DIP-5354N.
    IntActi P23337. 12 interactions.
    MINTi MINT-483654.
    STRINGi 4932.YFR015C.

    Protein family/group databases

    CAZyi GT3. Glycosyltransferase Family 3.

    Proteomic databases

    MaxQBi P23337.
    PaxDbi P23337.
    PeptideAtlasi P23337.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YFR015C ; YFR015C ; YFR015C .
    GeneIDi 850569.
    KEGGi sce:YFR015C.

    Organism-specific databases

    SGDi S000001911. GSY1.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00390000018612.
    HOGENOMi HOG000160890.
    KOi K00693.
    OMAi VSWEVAN.
    OrthoDBi EOG7QK0MF.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    BioCyci YEAST:YFR015C-MONOMER.

    Miscellaneous databases

    NextBioi 966381.

    Gene expression databases

    Genevestigatori P23337.

    Family and domain databases

    InterProi IPR008631. Glycogen_synth.
    [Graphical view ]
    PANTHERi PTHR10176. PTHR10176. 1 hit.
    Pfami PF05693. Glycogen_syn. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of the GSY1 gene encoding yeast glycogen synthase and evidence for the existence of a second gene."
      Farkas I., Hardy T.A., Depaoli-Roach A.A., Roach P.J.
      J. Biol. Chem. 265:20879-20886(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 2-17; 52-62; 86-105; 377-391; 593-600; 602-608 AND 660-669, CLEAVAGE OF INITIATOR METHIONINE.
      Strain: YPH52.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiGYS1_YEAST
    AccessioniPrimary (citable) accession number: P23337
    Secondary accession number(s): D6VTP6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 138 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VI
      Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

    External Data

    Dasty 3