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Protein

Glycogen [starch] synthase isoform 1

Gene

GSY1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan. Is believed to regulate the synthesis of glycogen.

Catalytic activityi

UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

Enzyme regulationi

Allosteric activation by glucose-6-phosphate, and phosphorylation by a cAMP-dependent kinase.

Pathway: glycogen biosynthesis

This protein is involved in the pathway glycogen biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei20 – 201UDP-glucoseBy similarity

GO - Molecular functioni

  • glycogen (starch) synthase activity Source: SGD

GO - Biological processi

  • glycogen biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Glycogen biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YFR015C-MONOMER.
ReactomeiREACT_331189. Glycogen synthesis.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycogen [starch] synthase isoform 1 (EC:2.4.1.11)
Gene namesi
Name:GSY1
Ordered Locus Names:YFR015C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFR015C.
SGDiS000001911. GSY1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 708707Glycogen [starch] synthase isoform 1PRO_0000194773Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei159 – 1591PhosphoserineSequence Analysis
Modified residuei363 – 3631PhosphoserineSequence Analysis
Modified residuei560 – 5601PhosphoserineSequence Analysis
Modified residuei651 – 6511Phosphoserine4 Publications
Modified residuei655 – 6551Phosphoserine5 Publications
Modified residuei660 – 6601Phosphoserine; by PKASequence Analysis
Modified residuei662 – 6621Phosphoserine; by PKASequence Analysis
Modified residuei667 – 6671PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23337.
PaxDbiP23337.
PeptideAtlasiP23337.

Expressioni

Developmental stagei

Activity increases just before cells entry in the stationary phase.

Inductioni

Synthesized in response to growth limitation.

Interactioni

Protein-protein interaction databases

BioGridi31167. 33 interactions.
DIPiDIP-5354N.
IntActiP23337. 12 interactions.
MINTiMINT-483654.
STRINGi4932.YFR015C.

Structurei

3D structure databases

ProteinModelPortaliP23337.
SMRiP23337. Positions 2-639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 3 family.Curated

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
InParanoidiP23337.
KOiK00693.
OMAiVCTIFTT.
OrthoDBiEOG7QK0MF.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23337-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARDLQNHLL FEVATEVTNR VGGIYSVLKS KAPVTVAQYG DNYTLLGPLN
60 70 80 90 100
KATYESEVEK LDWEDESIFP EELLPIQKTL MSMREKGVNF VYGNWLIEGA
110 120 130 140 150
PRVILFELDS VRHFLNEWKA DLWSLVGIPS PEHDHETNDA ILLGYVVVWF
160 170 180 190 200
LGEVSKLDSS HAIIGHFHEW LAGVALPLCR KKRIDVVTIF TTHATLLGRY
210 220 230 240 250
LCAAGDVDFY NNLQYFDVDQ EAGKRGIYHR YCIERAAAHT ADVFTTVSQI
260 270 280 290 300
TALEAEHLLK RKPDGILPNG LNVVKFQAVH EFQNLHALKK DKINDFVRGH
310 320 330 340 350
FHGCFDFDLD NTVYFFIAGR YEYKNKGADM FIESLARLNY RLKVSGSKKT
360 370 380 390 400
VVAFLIMPAK TNSFTVEALK SQAIVKSLEN TVNEVTASIG KRIFEHTMRY
410 420 430 440 450
PHNGLESELP TNLDELLKSS EKVLLKKRVL ALRRPYGELP PVVTHNMCDD
460 470 480 490 500
ANDPILNQIR HVRLFNDSSD RVKVIFHPEF LNANNPILGL DYDEFVRGCH
510 520 530 540 550
LGVFPSYYEP WGYTPAECTV MGVPSITTNV SGFGAYMEDL IETDQAKDYG
560 570 580 590 600
IYIVDRRFKS PDESVEQLAD YMEEFVNKTR RQRINQRNRT ERLSDLLDWK
610 620 630 640 650
RMGLEYVKAR QLGLRRAYPE QFKQLVGETI SDANMNTLAG GKKFKIARPL
660 670 680 690 700
SVPGSPKVRS NSTVYMTPGD LGTLQDANNA DDYFNLSTNG AIDNDDDDND

TSAYYEDN
Length:708
Mass (Da):80,510
Last modified:January 23, 2007 - v3
Checksum:i9EF2D3858529E68A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60919 Genomic DNA. Translation: AAA88715.1.
D50617 Genomic DNA. Translation: BAA09254.1.
BK006940 Genomic DNA. Translation: DAA12456.1.
PIRiA38326.
RefSeqiNP_116670.1. NM_001179980.1.

Genome annotation databases

EnsemblFungiiYFR015C; YFR015C; YFR015C.
GeneIDi850569.
KEGGisce:YFR015C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60919 Genomic DNA. Translation: AAA88715.1.
D50617 Genomic DNA. Translation: BAA09254.1.
BK006940 Genomic DNA. Translation: DAA12456.1.
PIRiA38326.
RefSeqiNP_116670.1. NM_001179980.1.

3D structure databases

ProteinModelPortaliP23337.
SMRiP23337. Positions 2-639.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31167. 33 interactions.
DIPiDIP-5354N.
IntActiP23337. 12 interactions.
MINTiMINT-483654.
STRINGi4932.YFR015C.

Protein family/group databases

CAZyiGT3. Glycosyltransferase Family 3.

Proteomic databases

MaxQBiP23337.
PaxDbiP23337.
PeptideAtlasiP23337.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYFR015C; YFR015C; YFR015C.
GeneIDi850569.
KEGGisce:YFR015C.

Organism-specific databases

EuPathDBiFungiDB:YFR015C.
SGDiS000001911. GSY1.

Phylogenomic databases

eggNOGiCOG0438.
GeneTreeiENSGT00390000018612.
HOGENOMiHOG000160890.
InParanoidiP23337.
KOiK00693.
OMAiVCTIFTT.
OrthoDBiEOG7QK0MF.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciYEAST:YFR015C-MONOMER.
ReactomeiREACT_331189. Glycogen synthesis.

Miscellaneous databases

NextBioi966381.
PROiP23337.

Family and domain databases

InterProiIPR008631. Glycogen_synth.
[Graphical view]
PANTHERiPTHR10176. PTHR10176. 1 hit.
PfamiPF05693. Glycogen_syn. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of the GSY1 gene encoding yeast glycogen synthase and evidence for the existence of a second gene."
    Farkas I., Hardy T.A., Depaoli-Roach A.A., Roach P.J.
    J. Biol. Chem. 265:20879-20886(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE OF 2-17; 52-62; 86-105; 377-391; 593-600; 602-608 AND 660-669, CLEAVAGE OF INITIATOR METHIONINE.
    Strain: YPH52.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-651 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiGYS1_YEAST
AccessioniPrimary (citable) accession number: P23337
Secondary accession number(s): D6VTP6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 147 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.