Reviewed, UniProtKB/Swiss-Prot P23336 (GGTA1_MOUSE)

Names and origin

Protein names

Recommended name:
    N-acetyllactosaminide alpha-1,3-galactosyltransferase
    EC=2.4.1.87
Alternative name(s):
    UDP-galactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase
      Short name=Galactosyltransferase

Gene names

Name:	Ggta1
Synonyms:	Ggta-1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Transfer of galactose from UDP-galactose to an acceptor molecule (R).
Catalytic activity	UDP-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Domain	The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with the beta-phosphate group of UDP and may also have a role in catalysis.
Sequence similarities	Belongs to the glycosyltransferase 6 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosaminide 3-alpha-galactosyltransferase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P23336-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P23336-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.

Isoform 3 (identifier: P23336-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-35: Missing.
     62-62: R → SPDGSFLWIYHTK

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	N-acetyllactosaminide alpha-1,3-galactosyltransferase		PRO_0000157299
Regions
	Topological domain	1 – 41	41	Cytoplasmic Potential
	Transmembrane	42 – 60	19	Signal-anchor for type II membrane protein Potential
	Topological domain	61 – 394	334	Lumenal Potential
Sites
	Metal binding	251	1	Manganese By similarity
	Metal binding	253	1	Manganese By similarity
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	319	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 35	35	Missing in isoform 2 and isoform 3.		VSP_001804
	Alternative sequence	62	1	R → SPDGSFLWIYHTK in isoform 3.		VSP_001805

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 7766831640D1BBF7	FASTA	394	46,475
	10 20 30 40 50 60 MITMLQDLHV NKISMSRSKS ETSLPSSRSG SQEKIMNVKG KVILLMLIVS TVVVVFWEYV 70 80 90 100 110 120 NRIPEVGENR WQKDWWFPSW FKNGTHSYQE DNVEGRREKG RNGDRIEEPQ LWDWFNPKNR 130 140 150 160 170 180 PDVLTVTPWK APIVWEGTYD TALLEKYYAT QKLTVGLTVF AVGKYIEHYL EDFLESADMY 190 200 210 220 230 240 FMVGHRVIFY VMIDDTSRMP VVHLNPLHSL QVFEIRSEKR WQDISMMRMK TIGEHILAHI 250 260 270 280 290 300 QHEVDFLFCM DVDQVFQDNF GVETLGQLVA QLQAWWYKAS PEKFTYERRE LSAAYIPFGE 310 320 330 340 350 360 GDFYYHAAIF GGTPTHILNL TRECFKGILQ DKKHDIEAQW HDESHLNKYF LFNKPTKILS 370 380 390 PEYCWDYQIG LPSDIKSVKV AWQTKEYNLV RNNV
	Isoform 2. Checksum: 2C74BE14EDCB0D8E	FASTA	359	42,586
	10 20 30 40 50 60 MNVKGKVILL MLIVSTVVVV FWEYVNRIPE VGENRWQKDW WFPSWFKNGT HSYQEDNVEG 70 80 90 100 110 120 RREKGRNGDR IEEPQLWDWF NPKNRPDVLT VTPWKAPIVW EGTYDTALLE KYYATQKLTV 130 140 150 160 170 180 GLTVFAVGKY IEHYLEDFLE SADMYFMVGH RVIFYVMIDD TSRMPVVHLN PLHSLQVFEI 190 200 210 220 230 240 RSEKRWQDIS MMRMKTIGEH ILAHIQHEVD FLFCMDVDQV FQDNFGVETL GQLVAQLQAW 250 260 270 280 290 300 WYKASPEKFT YERRELSAAY IPFGEGDFYY HAAIFGGTPT HILNLTRECF KGILQDKKHD 310 320 330 340 350 IEAQWHDESH LNKYFLFNKP TKILSPEYCW DYQIGLPSDI KSVKVAWQTK EYNLVRNNV
	Isoform 3. Checksum: D1281CD2292B5C19	FASTA	371	43,962
	10 20 30 40 50 60 MNVKGKVILL MLIVSTVVVV FWEYVNSPDG SFLWIYHTKI PEVGENRWQK DWWFPSWFKN 70 80 90 100 110 120 GTHSYQEDNV EGRREKGRNG DRIEEPQLWD WFNPKNRPDV LTVTPWKAPI VWEGTYDTAL 130 140 150 160 170 180 LEKYYATQKL TVGLTVFAVG KYIEHYLEDF LESADMYFMV GHRVIFYVMI DDTSRMPVVH 190 200 210 220 230 240 LNPLHSLQVF EIRSEKRWQD ISMMRMKTIG EHILAHIQHE VDFLFCMDVD QVFQDNFGVE 250 260 270 280 290 300 TLGQLVAQLQ AWWYKASPEK FTYERRELSA AYIPFGEGDF YYHAAIFGGT PTHILNLTRE 310 320 330 340 350 360 CFKGILQDKK HDIEAQWHDE SHLNKYFLFN KPTKILSPEY CWDYQIGLPS DIKSVKVAWQ 370 TKEYNLVRNN V

References

[1]	"Isolation of a cDNA encoding a murine UDPgalactose:beta-D-galactosyl-1,4-N-acetyl-D-glucosaminide alpha-1,3-galactosyltransferase: expression cloning by gene transfer." Larsen R.D., Rajan V.P., Ruff M.M., Kukowska-Latallo J., Cummings R.D., Lowe J.B. Proc. Natl. Acad. Sci. U.S.A. 86:8227-8231(1989) [PubMed: 2510162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Murine alpha 1,3-galactosyltransferase. A single gene locus specifies four isoforms of the enzyme by alternative splicing." Joziasse D.H., Shaper N.L., Kim D., van den Eijnden D.H., Shaper J.H. J. Biol. Chem. 267:5534-5541(1992) [PubMed: 1544928] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[3]	"Comparison of the regulatory regions of the alpha1,3galactosyltransferase gene between murine and porcine species." Koike C., Friday R., Fung J.J., Starzl T.E., Trucco M. Transplant. Proc. 33:710-711(2001) [PubMed: 11267031] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 3). Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26925 mRNA. Translation: AAA37657.1. M85153 mRNA. Translation: AAA37711.1. AF297614 , AF297609, AF297610, AF297611, AF297612, AF297613 Genomic DNA. Translation: AAK97205.1. AF297615 mRNA. Translation: AAK97206.1.
IPI	IPI00131261. IPI00226278. IPI00626100.
PIR	A34417. I49698.
RefSeq	NP_001139293.1.
UniGene	Mm.281124
3D structure databases
HSSP	HSSP built from PDB template 1GX4 based on UniProtKB P14769.
SMR	P23336. Positions 110-394.
ModBase	Search...
Protein-protein interaction databases
STRING	P23336.
Protein family/group databases
CAZy	GT6. Glycosyltransferase Family 6.
Genome annotation databases
Ensembl	ENSMUST00000102794; ENSMUSP00000099858; ENSMUSG00000035778; Mus musculus. [Genome view] ENSMUST00000113002; ENSMUSP00000108626; ENSMUSG00000035778; Mus musculus. [Genome view]
GeneID	14594.
KEGG	mmu:14594.
UCSC	uc008jkm.1. mouse.
Organism-specific databases
CTD	14594.
MGI	MGI:95704. Ggta1.
Phylogenomic databases
HOVERGEN	P23336.
Enzyme and pathway databases
BRENDA	2.4.1.87. 244.
Gene expression databases
ArrayExpress	P23336.
Bgee	P23336.
CleanEx	MM_GGTA1.
Genevestigator	P23336.
GermOnline	ENSMUSG00000035778. Mus musculus.
Family and domain databases
InterPro	IPR005076. Glyco_trans_6. [Graphical view]
PANTHER	PTHR10462. Glyco_trans_6. 1 hit.
Pfam	PF03414. Glyco_transf_6. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	286352.
SOURCE	Search...

Entry information

Entry name

GGTA1_MOUSE

Accession

Primary (citable) accession number: P23336
Secondary accession number(s): Q91V22

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	October 13, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)