P23335 (KITH_SWPVK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
ORF Names:	SWF8

Organism

Swinepox virus (strain Kasza) (SWPV)

Taxonomic identifier

10277 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Suipoxvirus › Swinepox virus

Virus host

Sus scrofa (Pig) [TaxID: 9823]

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.
Sequence caution	The sequence AAA47894.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Thymidine kinase		PRO_0000174936
Regions
	Nucleotide binding	11 – 18	8	ATP By similarity
	Region	158 – 162	5	Substrate binding By similarity
Sites
	Active site	84	1	Proton acceptor Potential
	Metal binding	139	1	Zinc By similarity
	Metal binding	142	1	Zinc By similarity
	Metal binding	171	1	Zinc By similarity
	Metal binding	174	1	Zinc By similarity
	Binding site	114	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23335 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 91694E4A1B4CB942	FASTA	177	20,053
	10 20 30 40 50 60 MSCGFIHLIL GPMFSGKSTE LIRLVNRYQI ATYNCRVIKY SKDNRYGNDA VYTHDKCYIS 70 80 90 100 110 120 AVSTDSLFDI KDTLDDVDIV GIDEGQFFND IVEFCEYIAN KGKIVIVAAL DGTYERKPFG 130 140 150 160 170 NILNLIPLSE KVTKLNAICM ICHRDASFSK RLSDEKEIEL IGGKEKYLSV CRSCYLT

References

[1]	"Isolation and molecular characterization of the swinepox virus thymidine kinase gene." Feller J.A., Massung R.F., Turner P.C., Gibbs E.P.J., Bockamp E.O., Beloso A., Talavera A., Vinuela E., Moyer R.W. Virology 183:578-585(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification and nucleotide sequence of the thymidine kinase gene of swinepox virus." Schnitzlein W.M., Tripathy D.N. Virology 181:727-732(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64000 Genomic DNA. Translation: AAA47894.1. Different initiation. M59931 Genomic DNA. Translation: AAA47891.1.
PIR	KIVZSW. B37949.
3D structure databases
ProteinModelPortal	P23335.
SMR	P23335. Positions 4-176.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_SWPVK

Accession

Primary (citable) accession number: P23335

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program