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P23331

- KITH_ECOLI

UniProt

P23331 - KITH_ECOLI

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Protein

Thymidine kinase

Gene

tdk

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Phosphorylates both thymidine and deoxyuridine.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Enzyme regulationi

Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Proton acceptorUniRule annotation
Metal bindingi145 – 1451ZincUniRule annotation
Metal bindingi147 – 1471ZincUniRule annotation
Metal bindingi182 – 1821ZincUniRule annotation
Metal bindingi185 – 1851ZincUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 168ATPCurated
Nucleotide bindingi87 – 904ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. thymidine kinase activity Source: EcoCyc
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
  2. DNA metabolic process Source: EcoCyc
  3. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:TDK-MONOMER.
ECOL316407:JW1226-MONOMER.
MetaCyc:TDK-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:tdkUniRule annotation
Ordered Locus Names:b1238, JW1226
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10994. tdk.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Thymidine kinasePRO_0000174971Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP23331.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

IntActiP23331. 6 interactions.
STRINGi511145.b1238.

Structurei

3D structure databases

ProteinModelPortaliP23331.
SMRiP23331. Positions 6-187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076391.
InParanoidiP23331.
KOiK00857.
OMAiELKTVCH.
OrthoDBiEOG69D3J2.
PhylomeDBiP23331.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23331-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS
60 70 80 90 100
SRIGLSSPAK LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL
110 120 130 140 150
SEVVDQLDIP VLCYGLRTDF RGELFIGSQY LLAWSDKLVE LKTICFCGRK
160 170 180 190 200
ASMVLRLDQA GRPYNEGEQV VIGGNERYVS VCRKHYKEAL QVDSLTAIQE

RHRHD
Length:205
Mass (Da):23,457
Last modified:November 1, 1991 - v1
Checksum:i533166D9EAD20E16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51523 Genomic DNA. Translation: CAA35907.1.
X53733 Genomic DNA. Translation: CAA37765.1.
X67326 Genomic DNA. Translation: CAA47741.1.
DQ384607 Genomic DNA. Translation: ABD37701.1.
U00096 Genomic DNA. Translation: AAC74320.1.
AP009048 Genomic DNA. Translation: BAA36118.1.
PIRiJS0519.
RefSeqiNP_415754.1. NC_000913.3.
YP_489506.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74320; AAC74320; b1238.
BAA36118; BAA36118; BAA36118.
GeneIDi12930562.
945834.
KEGGiecj:Y75_p1211.
eco:b1238.
PATRICi32117732. VBIEscCol129921_1286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51523 Genomic DNA. Translation: CAA35907.1 .
X53733 Genomic DNA. Translation: CAA37765.1 .
X67326 Genomic DNA. Translation: CAA47741.1 .
DQ384607 Genomic DNA. Translation: ABD37701.1 .
U00096 Genomic DNA. Translation: AAC74320.1 .
AP009048 Genomic DNA. Translation: BAA36118.1 .
PIRi JS0519.
RefSeqi NP_415754.1. NC_000913.3.
YP_489506.1. NC_007779.1.

3D structure databases

ProteinModelPortali P23331.
SMRi P23331. Positions 6-187.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P23331. 6 interactions.
STRINGi 511145.b1238.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74320 ; AAC74320 ; b1238 .
BAA36118 ; BAA36118 ; BAA36118 .
GeneIDi 12930562.
945834.
KEGGi ecj:Y75_p1211.
eco:b1238.
PATRICi 32117732. VBIEscCol129921_1286.

Organism-specific databases

EchoBASEi EB0987.
EcoGenei EG10994. tdk.

Phylogenomic databases

eggNOGi COG1435.
HOGENOMi HOG000076391.
InParanoidi P23331.
KOi K00857.
OMAi ELKTVCH.
OrthoDBi EOG69D3J2.
PhylomeDBi P23331.

Enzyme and pathway databases

BioCyci EcoCyc:TDK-MONOMER.
ECOL316407:JW1226-MONOMER.
MetaCyc:TDK-MONOMER.

Miscellaneous databases

PROi P23331.

Gene expression databases

Genevestigatori P23331.

Family and domain databases

HAMAPi MF_00124. Thymidine_kinase.
InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Escherichia coli thymidine kinase: nucleotide sequence of the gene and relationships to other thymidine kinases."
    Bockamp E.O., Blasco R., Vinuela E.
    Gene 101:9-14(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Nucleotide sequence of the Escherichia coli thymidine kinase gene provides evidence for conservation of functional domains and quaternary structure."
    Black M.E., Hruby D.E.
    Mol. Microbiol. 5:373-379(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Filling the gap between hns and adhE in Escherichia coli K12."
    Danchin A., Krin E.
    Microbiology 141:959-960(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  4. "Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner."
    Sandrini M.P., Clausen A.R., On S.L., Aarestrup F.M., Munch-Petersen B., Piskur J.
    J. Antimicrob. Chemother. 60:510-520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiKITH_ECOLI
AccessioniPrimary (citable) accession number: P23331
Secondary accession number(s): Q0H0G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3