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P23331 (KITH_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidine kinase

EC=2.7.1.21
Gene names
Name:tdk
Ordered Locus Names:b1238, JW1226
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length205 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Phosphorylates both thymidine and deoxyuridine. HAMAP-Rule MF_00124

Catalytic activity

ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124

Enzyme regulation

Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP. HAMAP-Rule MF_00124

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00124

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00124.

Sequence similarities

Belongs to the thymidine kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 205205Thymidine kinase HAMAP-Rule MF_00124
PRO_0000174971

Regions

Nucleotide binding9 – 168ATP Probable
Nucleotide binding87 – 904ATP By similarity

Sites

Active site881Proton acceptor Potential
Metal binding1451Zinc By similarity
Metal binding1471Zinc By similarity
Metal binding1821Zinc By similarity
Metal binding1851Zinc By similarity

Sequences

Sequence LengthMass (Da)Tools
P23331 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 533166D9EAD20E16

FASTA20523,457
        10         20         30         40         50         60 
MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS SRIGLSSPAK 

        70         80         90        100        110        120 
LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL SEVVDQLDIP VLCYGLRTDF 

       130        140        150        160        170        180 
RGELFIGSQY LLAWSDKLVE LKTICFCGRK ASMVLRLDQA GRPYNEGEQV VIGGNERYVS 

       190        200 
VCRKHYKEAL QVDSLTAIQE RHRHD 

« Hide

References

« Hide 'large scale' references
[1]"Escherichia coli thymidine kinase: nucleotide sequence of the gene and relationships to other thymidine kinases."
Bockamp E.O., Blasco R., Vinuela E.
Gene 101:9-14(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Nucleotide sequence of the Escherichia coli thymidine kinase gene provides evidence for conservation of functional domains and quaternary structure."
Black M.E., Hruby D.E.
Mol. Microbiol. 5:373-379(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Filling the gap between hns and adhE in Escherichia coli K12."
Danchin A., Krin E.
Microbiology 141:959-960(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[4]"Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner."
Sandrini M.P., Clausen A.R., On S.L., Aarestrup F.M., Munch-Petersen B., Piskur J.
J. Antimicrob. Chemother. 60:510-520(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[5]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[7]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X51523 Genomic DNA. Translation: CAA35907.1.
X53733 Genomic DNA. Translation: CAA37765.1.
X67326 Genomic DNA. Translation: CAA47741.1.
DQ384607 Genomic DNA. Translation: ABD37701.1.
U00096 Genomic DNA. Translation: AAC74320.1.
AP009048 Genomic DNA. Translation: BAA36118.1.
PIRJS0519.
RefSeqNP_415754.1. NC_000913.3.
YP_489506.1. NC_007779.1.

3D structure databases

ProteinModelPortalP23331.
SMRP23331. Positions 6-187.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP23331. 6 interactions.
STRING511145.b1238.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74320; AAC74320; b1238.
BAA36118; BAA36118; BAA36118.
GeneID12930562.
945834.
KEGGecj:Y75_p1211.
eco:b1238.
PATRIC32117732. VBIEscCol129921_1286.

Organism-specific databases

EchoBASEEB0987.
EcoGeneEG10994. tdk.

Phylogenomic databases

eggNOGCOG1435.
HOGENOMHOG000076391.
KOK00857.
OMARTICHCG.
OrthoDBEOG69D3J2.
PhylomeDBP23331.
ProtClustDBPRK04296.

Enzyme and pathway databases

BioCycEcoCyc:TDK-MONOMER.
ECOL316407:JW1226-MONOMER.
MetaCyc:TDK-MONOMER.

Gene expression databases

GenevestigatorP23331.

Family and domain databases

HAMAPMF_00124. Thymidine_kinase.
InterProIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERPTHR11441. PTHR11441. 1 hit.
PfamPF00265. TK. 1 hit.
[Graphical view]
PIRSFPIRSF035805. TK_cell. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP23331.

Entry information

Entry nameKITH_ECOLI
AccessionPrimary (citable) accession number: P23331
Secondary accession number(s): Q0H0G7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene