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P23331

- KITH_ECOLI

UniProt

P23331 - KITH_ECOLI

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Protein
Thymidine kinase
Gene
tdk, b1238, JW1226
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Phosphorylates both thymidine and deoxyuridine.UniRule annotation

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Enzyme regulationi

Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Proton acceptor Reviewed prediction
Metal bindingi145 – 1451Zinc By similarity
Metal bindingi147 – 1471Zinc By similarity
Metal bindingi182 – 1821Zinc By similarity
Metal bindingi185 – 1851Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 168ATP Inferred
Nucleotide bindingi87 – 904ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. thymidine kinase activity Source: EcoCyc
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA metabolic process Source: EcoCyc
  2. DNA replication Source: UniProtKB-KW
  3. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:TDK-MONOMER.
ECOL316407:JW1226-MONOMER.
MetaCyc:TDK-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:tdk
Ordered Locus Names:b1238, JW1226
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10994. tdk.

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Thymidine kinaseUniRule annotation
PRO_0000174971Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP23331.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

IntActiP23331. 6 interactions.
STRINGi511145.b1238.

Structurei

3D structure databases

ProteinModelPortaliP23331.
SMRiP23331. Positions 6-187.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076391.
KOiK00857.
OMAiELKTVCH.
OrthoDBiEOG69D3J2.
PhylomeDBiP23331.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23331-1 [UniParc]FASTAAdd to Basket

« Hide

MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS    50
SRIGLSSPAK LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL 100
SEVVDQLDIP VLCYGLRTDF RGELFIGSQY LLAWSDKLVE LKTICFCGRK 150
ASMVLRLDQA GRPYNEGEQV VIGGNERYVS VCRKHYKEAL QVDSLTAIQE 200
RHRHD 205
Length:205
Mass (Da):23,457
Last modified:November 1, 1991 - v1
Checksum:i533166D9EAD20E16
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51523 Genomic DNA. Translation: CAA35907.1.
X53733 Genomic DNA. Translation: CAA37765.1.
X67326 Genomic DNA. Translation: CAA47741.1.
DQ384607 Genomic DNA. Translation: ABD37701.1.
U00096 Genomic DNA. Translation: AAC74320.1.
AP009048 Genomic DNA. Translation: BAA36118.1.
PIRiJS0519.
RefSeqiNP_415754.1. NC_000913.3.
YP_489506.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74320; AAC74320; b1238.
BAA36118; BAA36118; BAA36118.
GeneIDi12930562.
945834.
KEGGiecj:Y75_p1211.
eco:b1238.
PATRICi32117732. VBIEscCol129921_1286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X51523 Genomic DNA. Translation: CAA35907.1 .
X53733 Genomic DNA. Translation: CAA37765.1 .
X67326 Genomic DNA. Translation: CAA47741.1 .
DQ384607 Genomic DNA. Translation: ABD37701.1 .
U00096 Genomic DNA. Translation: AAC74320.1 .
AP009048 Genomic DNA. Translation: BAA36118.1 .
PIRi JS0519.
RefSeqi NP_415754.1. NC_000913.3.
YP_489506.1. NC_007779.1.

3D structure databases

ProteinModelPortali P23331.
SMRi P23331. Positions 6-187.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P23331. 6 interactions.
STRINGi 511145.b1238.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74320 ; AAC74320 ; b1238 .
BAA36118 ; BAA36118 ; BAA36118 .
GeneIDi 12930562.
945834.
KEGGi ecj:Y75_p1211.
eco:b1238.
PATRICi 32117732. VBIEscCol129921_1286.

Organism-specific databases

EchoBASEi EB0987.
EcoGenei EG10994. tdk.

Phylogenomic databases

eggNOGi COG1435.
HOGENOMi HOG000076391.
KOi K00857.
OMAi ELKTVCH.
OrthoDBi EOG69D3J2.
PhylomeDBi P23331.

Enzyme and pathway databases

BioCyci EcoCyc:TDK-MONOMER.
ECOL316407:JW1226-MONOMER.
MetaCyc:TDK-MONOMER.

Miscellaneous databases

PROi P23331.

Gene expression databases

Genevestigatori P23331.

Family and domain databases

HAMAPi MF_00124. Thymidine_kinase.
InterProi IPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
IPR020634. Thymidine_kinase_subgr.
[Graphical view ]
PANTHERi PTHR11441. PTHR11441. 1 hit.
Pfami PF00265. TK. 1 hit.
[Graphical view ]
PIRSFi PIRSF035805. TK_cell. 1 hit.
SUPFAMi SSF52540. SSF52540. 1 hit.
PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Escherichia coli thymidine kinase: nucleotide sequence of the gene and relationships to other thymidine kinases."
    Bockamp E.O., Blasco R., Vinuela E.
    Gene 101:9-14(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Nucleotide sequence of the Escherichia coli thymidine kinase gene provides evidence for conservation of functional domains and quaternary structure."
    Black M.E., Hruby D.E.
    Mol. Microbiol. 5:373-379(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Filling the gap between hns and adhE in Escherichia coli K12."
    Danchin A., Krin E.
    Microbiology 141:959-960(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  4. "Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner."
    Sandrini M.P., Clausen A.R., On S.L., Aarestrup F.M., Munch-Petersen B., Piskur J.
    J. Antimicrob. Chemother. 60:510-520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiKITH_ECOLI
AccessioniPrimary (citable) accession number: P23331
Secondary accession number(s): Q0H0G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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