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Protein

Thymidine kinase

Gene

tdk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Phosphorylates both thymidine and deoxyuridine.

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

Enzyme regulationi

Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Proton acceptorUniRule annotation
Metal bindingi145 – 1451ZincUniRule annotation
Metal bindingi147 – 1471ZincUniRule annotation
Metal bindingi182 – 1821ZincUniRule annotation
Metal bindingi185 – 1851ZincUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 168ATPCurated
Nucleotide bindingi87 – 904ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. thymidine kinase activity Source: EcoCyc
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
  2. DNA biosynthetic process Source: UniProtKB-KW
  3. DNA metabolic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:TDK-MONOMER.
ECOL316407:JW1226-MONOMER.
MetaCyc:TDK-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
Gene namesi
Name:tdkUniRule annotation
Ordered Locus Names:b1238, JW1226
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10994. tdk.

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Thymidine kinasePRO_0000174971Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP23331.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

IntActiP23331. 6 interactions.
STRINGi511145.b1238.

Structurei

3D structure databases

ProteinModelPortaliP23331.
SMRiP23331. Positions 6-187.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidine kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076391.
InParanoidiP23331.
KOiK00857.
OMAiFFEYGAM.
OrthoDBiEOG69D3J2.
PhylomeDBiP23331.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS
60 70 80 90 100
SRIGLSSPAK LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL
110 120 130 140 150
SEVVDQLDIP VLCYGLRTDF RGELFIGSQY LLAWSDKLVE LKTICFCGRK
160 170 180 190 200
ASMVLRLDQA GRPYNEGEQV VIGGNERYVS VCRKHYKEAL QVDSLTAIQE

RHRHD
Length:205
Mass (Da):23,457
Last modified:November 1, 1991 - v1
Checksum:i533166D9EAD20E16
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51523 Genomic DNA. Translation: CAA35907.1.
X53733 Genomic DNA. Translation: CAA37765.1.
X67326 Genomic DNA. Translation: CAA47741.1.
DQ384607 Genomic DNA. Translation: ABD37701.1.
U00096 Genomic DNA. Translation: AAC74320.1.
AP009048 Genomic DNA. Translation: BAA36118.1.
PIRiJS0519.
RefSeqiNP_415754.1. NC_000913.3.
YP_489506.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74320; AAC74320; b1238.
BAA36118; BAA36118; BAA36118.
GeneIDi12930562.
945834.
KEGGiecj:Y75_p1211.
eco:b1238.
PATRICi32117732. VBIEscCol129921_1286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X51523 Genomic DNA. Translation: CAA35907.1.
X53733 Genomic DNA. Translation: CAA37765.1.
X67326 Genomic DNA. Translation: CAA47741.1.
DQ384607 Genomic DNA. Translation: ABD37701.1.
U00096 Genomic DNA. Translation: AAC74320.1.
AP009048 Genomic DNA. Translation: BAA36118.1.
PIRiJS0519.
RefSeqiNP_415754.1. NC_000913.3.
YP_489506.1. NC_007779.1.

3D structure databases

ProteinModelPortaliP23331.
SMRiP23331. Positions 6-187.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP23331. 6 interactions.
STRINGi511145.b1238.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74320; AAC74320; b1238.
BAA36118; BAA36118; BAA36118.
GeneIDi12930562.
945834.
KEGGiecj:Y75_p1211.
eco:b1238.
PATRICi32117732. VBIEscCol129921_1286.

Organism-specific databases

EchoBASEiEB0987.
EcoGeneiEG10994. tdk.

Phylogenomic databases

eggNOGiCOG1435.
HOGENOMiHOG000076391.
InParanoidiP23331.
KOiK00857.
OMAiFFEYGAM.
OrthoDBiEOG69D3J2.
PhylomeDBiP23331.

Enzyme and pathway databases

BioCyciEcoCyc:TDK-MONOMER.
ECOL316407:JW1226-MONOMER.
MetaCyc:TDK-MONOMER.

Miscellaneous databases

PROiP23331.

Gene expression databases

GenevestigatoriP23331.

Family and domain databases

HAMAPiMF_00124. Thymidine_kinase.
InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Escherichia coli thymidine kinase: nucleotide sequence of the gene and relationships to other thymidine kinases."
    Bockamp E.O., Blasco R., Vinuela E.
    Gene 101:9-14(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Nucleotide sequence of the Escherichia coli thymidine kinase gene provides evidence for conservation of functional domains and quaternary structure."
    Black M.E., Hruby D.E.
    Mol. Microbiol. 5:373-379(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Filling the gap between hns and adhE in Escherichia coli K12."
    Danchin A., Krin E.
    Microbiology 141:959-960(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  4. "Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner."
    Sandrini M.P., Clausen A.R., On S.L., Aarestrup F.M., Munch-Petersen B., Piskur J.
    J. Antimicrob. Chemother. 60:510-520(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiKITH_ECOLI
AccessioniPrimary (citable) accession number: P23331
Secondary accession number(s): Q0H0G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 1, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.