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P23331

- KITH_ECOLI

UniProt

P23331 - KITH_ECOLI

Protein

Thymidine kinase

Gene

tdk

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Phosphorylates both thymidine and deoxyuridine.

    Catalytic activityi

    ATP + thymidine = ADP + thymidine 5'-phosphate.UniRule annotation

    Enzyme regulationi

    Allosteric enzyme which is feedback inhibited by dTTP and activated by a number of dNDP and dNTP.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Proton acceptorUniRule annotation
    Metal bindingi145 – 1451ZincUniRule annotation
    Metal bindingi147 – 1471ZincUniRule annotation
    Metal bindingi182 – 1821ZincUniRule annotation
    Metal bindingi185 – 1851ZincUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi9 – 168ATPCurated
    Nucleotide bindingi87 – 904ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. thymidine kinase activity Source: EcoCyc
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. deoxyribonucleoside monophosphate biosynthetic process Source: GOC
    2. DNA metabolic process Source: EcoCyc
    3. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    DNA synthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:TDK-MONOMER.
    ECOL316407:JW1226-MONOMER.
    MetaCyc:TDK-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Thymidine kinaseUniRule annotation (EC:2.7.1.21UniRule annotation)
    Gene namesi
    Name:tdkUniRule annotation
    Ordered Locus Names:b1238, JW1226
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10994. tdk.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 205205Thymidine kinasePRO_0000174971Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP23331.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    IntActiP23331. 6 interactions.
    STRINGi511145.b1238.

    Structurei

    3D structure databases

    ProteinModelPortaliP23331.
    SMRiP23331. Positions 6-187.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thymidine kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1435.
    HOGENOMiHOG000076391.
    KOiK00857.
    OMAiELKTVCH.
    OrthoDBiEOG69D3J2.
    PhylomeDBiP23331.

    Family and domain databases

    HAMAPiMF_00124. Thymidine_kinase.
    InterProiIPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view]
    PANTHERiPTHR11441. PTHR11441. 1 hit.
    PfamiPF00265. TK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF035805. TK_cell. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23331-1 [UniParc]FASTAAdd to Basket

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    MAQLYFYYSA MNAGKSTALL QSSYNYQERG MRTVVYTAEI DDRFGAGKVS    50
    SRIGLSSPAK LFNQNSSLFD EIRAEHEQQA IHCVLVDECQ FLTRQQVYEL 100
    SEVVDQLDIP VLCYGLRTDF RGELFIGSQY LLAWSDKLVE LKTICFCGRK 150
    ASMVLRLDQA GRPYNEGEQV VIGGNERYVS VCRKHYKEAL QVDSLTAIQE 200
    RHRHD 205
    Length:205
    Mass (Da):23,457
    Last modified:November 1, 1991 - v1
    Checksum:i533166D9EAD20E16
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51523 Genomic DNA. Translation: CAA35907.1.
    X53733 Genomic DNA. Translation: CAA37765.1.
    X67326 Genomic DNA. Translation: CAA47741.1.
    DQ384607 Genomic DNA. Translation: ABD37701.1.
    U00096 Genomic DNA. Translation: AAC74320.1.
    AP009048 Genomic DNA. Translation: BAA36118.1.
    PIRiJS0519.
    RefSeqiNP_415754.1. NC_000913.3.
    YP_489506.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74320; AAC74320; b1238.
    BAA36118; BAA36118; BAA36118.
    GeneIDi12930562.
    945834.
    KEGGiecj:Y75_p1211.
    eco:b1238.
    PATRICi32117732. VBIEscCol129921_1286.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X51523 Genomic DNA. Translation: CAA35907.1 .
    X53733 Genomic DNA. Translation: CAA37765.1 .
    X67326 Genomic DNA. Translation: CAA47741.1 .
    DQ384607 Genomic DNA. Translation: ABD37701.1 .
    U00096 Genomic DNA. Translation: AAC74320.1 .
    AP009048 Genomic DNA. Translation: BAA36118.1 .
    PIRi JS0519.
    RefSeqi NP_415754.1. NC_000913.3.
    YP_489506.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P23331.
    SMRi P23331. Positions 6-187.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P23331. 6 interactions.
    STRINGi 511145.b1238.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74320 ; AAC74320 ; b1238 .
    BAA36118 ; BAA36118 ; BAA36118 .
    GeneIDi 12930562.
    945834.
    KEGGi ecj:Y75_p1211.
    eco:b1238.
    PATRICi 32117732. VBIEscCol129921_1286.

    Organism-specific databases

    EchoBASEi EB0987.
    EcoGenei EG10994. tdk.

    Phylogenomic databases

    eggNOGi COG1435.
    HOGENOMi HOG000076391.
    KOi K00857.
    OMAi ELKTVCH.
    OrthoDBi EOG69D3J2.
    PhylomeDBi P23331.

    Enzyme and pathway databases

    BioCyci EcoCyc:TDK-MONOMER.
    ECOL316407:JW1226-MONOMER.
    MetaCyc:TDK-MONOMER.

    Miscellaneous databases

    PROi P23331.

    Gene expression databases

    Genevestigatori P23331.

    Family and domain databases

    HAMAPi MF_00124. Thymidine_kinase.
    InterProi IPR027417. P-loop_NTPase.
    IPR001267. Thymidine_kinase.
    IPR020633. Thymidine_kinase_CS.
    IPR020634. Thymidine_kinase_subgr.
    [Graphical view ]
    PANTHERi PTHR11441. PTHR11441. 1 hit.
    Pfami PF00265. TK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF035805. TK_cell. 1 hit.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00603. TK_CELLULAR_TYPE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Escherichia coli thymidine kinase: nucleotide sequence of the gene and relationships to other thymidine kinases."
      Bockamp E.O., Blasco R., Vinuela E.
      Gene 101:9-14(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Nucleotide sequence of the Escherichia coli thymidine kinase gene provides evidence for conservation of functional domains and quaternary structure."
      Black M.E., Hruby D.E.
      Mol. Microbiol. 5:373-379(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Filling the gap between hns and adhE in Escherichia coli K12."
      Danchin A., Krin E.
      Microbiology 141:959-960(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    4. "Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific manner."
      Sandrini M.P., Clausen A.R., On S.L., Aarestrup F.M., Munch-Petersen B., Piskur J.
      J. Antimicrob. Chemother. 60:510-520(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    7. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiKITH_ECOLI
    AccessioniPrimary (citable) accession number: P23331
    Secondary accession number(s): Q0H0G7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3