Reviewed, UniProtKB/Swiss-Prot P23321 (PSBO1_ARATH)

Names and origin

Protein names

Recommended name:
    Oxygen-evolving enhancer protein 1-1, chloroplastic
      Short name=OEE1
Alternative name(s):
    33 kDa subunit of oxygen evolving system of photosystem II
    OEC 33 kDa subunit
    33 kDa thylakoid membrane protein
    Manganese-stabilizing protein 1
      Short name=MSP-1

Gene names

Name:	PSBO1
Synonyms:	PSBO
Ordered Locus Names:	At5g66570
ORF Names:	K1F13.25

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Stabilizes the manganese cluster which is the primary site of water splitting. Ref.9 Ref.10
Subcellular location	Plastid › chloroplast thylakoid membrane. Note: Associated with the photosystem II complex. Ref.7
Tissue specificity	Leaves.
Miscellaneous	PSBO1 is the major isoform in the wild-type and it cannot be fully complemented by PSBO2.
Sequence similarities	Belongs to the psbO family.
Sequence caution	The sequence CAA79171.1 differs from that shown. Reason: Frameshift at positions 130 and 162.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Membrane Photosystem II Plastid Thylakoid
Domain	Transit peptide
Ligand	Manganese
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from expression pattern. Source: TAIR photoinhibition Inferred from mutant phenotype. Source: TAIR photosystem II assembly Ref.10 Inferred from mutant phenotype. Source: TAIR photosystem II stabilization Ref.10 Inferred from mutant phenotype. Source: TAIR regulation of protein amino acid dephosphorylation Inferred from mutant phenotype. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast stroma Inferred from direct assay. Source: TAIR chloroplast thylakoid lumen Inferred from direct assay. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR extrinsic to membrane Inferred from electronic annotation. Source: InterPro oxygen evolving complex Inferred from electronic annotation. Source: InterPro plastoglobule Inferred from direct assay. Source: TAIR
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxygen evolving activity Inferred from direct assay. Source: TAIR poly(U) binding Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TRX3	Q42403	1	EBI-449414,EBI-449157

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 58	58	Chloroplast
	Transit peptide	59 – 85	27	Thylakoid
	Chain	86 – 332	247	Oxygen-evolving enhancer protein 1-1, chloroplastic		PRO_0000029553
Experimental info
	Sequence conflict	57	1	D → N in AAN15726. Ref.4
	Sequence conflict	57	1	D → N in AAM96957. Ref.4
	Sequence conflict	75	1	T → S in AAL08257. Ref.4
	Sequence conflict	113 – 114	2	QC → HS in CAA36675. Ref.1
	Sequence conflict	132	1	A → S in AAM65169. Ref.5
	Sequence conflict	206	1	G → C in AAK96774. Ref.4
	Sequence conflict	278	1	T → S in AAK49614. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P23321-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: 73174DDC09C11E5C	FASTA	332	35,142
	10 20 30 40 50 60 MAASLQSTAT FLQSAKIATA PSRGSSHLRS TQAVGKSFGL ETSSARLTCS FQSDFKDFTG 70 80 90 100 110 120 KCSDAVKIAG FALATSALVV SGASAEGAPK RLTYDEIQSK TYMEVKGTGT ANQCPTIDGG 130 140 150 160 170 180 SETFSFKPGK YAGKKFCFEP TSFTVKADSV SKNAPPEFQN TKLMTRLTYT LDEIEGPFEV 190 200 210 220 230 240 ASDGSVNFKE EDGIDYAAVT VQLPGGERVP FLFTVKQLDA SGKPDSFTGK FLVPSYRGSS 250 260 270 280 290 300 FLDPKGRGGS TGYDNAVALP AGGRGDEEEL VKENVKNTAA SVGEITLKVT KSKPETGEVI 310 320 330 GVFESLQPSD TDLGAKVPKD VKIQGVWYGQ LE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cDNAs from Lycopersicon esculentum and Arabidopsis thaliana encoding the 33 kDa protein of the photosystem II-associated oxygen-evolving complex." Ko K., Granell A., Bennett J., Cashmore A.R. Plant Mol. Biol. 14:217-227(1990) [PubMed: 2101691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The psbO gene for 33-kDa precursor polypeptide of the oxygen-evolving complex in Arabidopsis thaliana -- nucleotide sequence and control of its expression." Jain P.K., Kochhar A., Khurana J.P., Tyagi A.K. DNA Res. 5:221-228(1998) [PubMed: 9802567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana." Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. , Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C., Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B. Plant J. 4:1051-1061(1993) [PubMed: 8281187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 130-179. Strain: cv. C24. Tissue: Flower bud.
[7]	"Targeting of proteins to the thylakoid lumen by the bipartite transit peptide of the 33 kd oxygen-evolving protein." Ko K., Cashmore A.R. EMBO J. 8:3187-3194(1989) [PubMed: 2583096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-90, SUBCELLULAR LOCATION.
[8]	"Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction." Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G., Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G., van Wijk K.J. Plant Cell 14:211-236(2002) [PubMed: 11826309] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY.
[9]	"Characterization of an Arabidopsis thaliana mutant with impaired psbO, one of two genes encoding extrinsic 33-kDa proteins in photosystem II." Murakami R., Ifuku K., Takabayashi A., Shikanai T., Endo T., Sato F. FEBS Lett. 523:138-142(2002) [PubMed: 12123820] [Abstract] Cited for: FUNCTION.
[10]	"The manganese-stabilizing protein is required for photosystem II assembly/stability and photoautotrophy in higher plants." Yi X., McChargue M., Laborde S., Frankel L.K., Bricker T.M. J. Biol. Chem. 280:16170-16174(2005) [PubMed: 15722336] [Abstract] Cited for: FUNCTION.
[11]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed: 18431481] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52428 mRNA. Translation: CAA36675.1. Y15433 Genomic DNA. Translation: CAA75629.1. AB013389 Genomic DNA. Translation: BAB10933.1. AF424626 mRNA. Translation: AAL11619.1. AY052299 mRNA. Translation: AAK96492.1. AY061924 mRNA. Translation: AAL31251.1. AF372898 mRNA. Translation: AAK49614.1. AY136291 mRNA. Translation: AAM96957.1. AY054583 mRNA. Translation: AAK96774.1. AY056401 mRNA. Translation: AAL08257.1. BT000407 mRNA. Translation: AAN15726.1. AY087630 mRNA. Translation: AAM65169.1. Z18374 mRNA. Translation: CAA79171.1. Frameshift.
IPI	IPI00545883.
PIR	S11852.
RefSeq	NP_201458.1.
UniGene	At.24135 At.25361 At.71536 At.71712 At.72732
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P23321. 1 interaction.
2-D gel databases
SWISS-2DPAGE	P23321.
Proteomic databases
PRIDE	P23321.
Genome annotation databases
GeneID	836789.
GenomeReviews	Gene locus AT5G66570 in contig BA000015_GR.
KEGG	ath:AT5G66570.
NMPDR	fig|3702.1.peg.28761.
Organism-specific databases
TAIR	At5g66570.
Phylogenomic databases
OMA	P23321. SKNAPPE.
Gene expression databases
GermOnline	AT5G66570. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002628. PSII_MSP. [Graphical view]
Pfam	PF01716. MSP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSBO1_ARATH

Accession

Primary (citable) accession number: P23321
Secondary accession number(s): O81917 , Q41998, Q8L7F1, Q8LAT1, Q93Y28, Q93ZP8, Q94JV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)