Oxygen-evolving enhancer protein 1-1, chloroplastic precursor - Arabidopsis thaliana (Mouse-ear cress)

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P23321 (PSBO1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Oxygen-evolving enhancer protein 1-1, chloroplastic
Short name=OEE1

Alternative name(s):
33 kDa subunit of oxygen evolving system of photosystem II
33 kDa thylakoid membrane protein
Manganese-stabilizing protein 1
Short name=MSP-1
OEC 33 kDa subunit

Gene names

Name:	PSBO1
Synonyms:	PSBO
Ordered Locus Names:	At5g66570
ORF Names:	K1F13.25

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	332 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Stabilizes the manganese cluster which is the primary site of water splitting. Ref.10 Ref.11
Subcellular location	Plastid › chloroplast thylakoid membrane. Note: Associated with the photosystem II complex. Ref.8
Tissue specificity	Leaves.
Miscellaneous	PSBO1 is the major isoform in the wild-type and it cannot be fully complemented by PSBO2.
Sequence similarities	Belongs to the psbO family.
Sequence caution	The sequence CAA79171.1 differs from that shown. Reason: Frameshift at positions 130 and 162.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Membrane Photosystem II Plastid Thylakoid
Domain	Transit peptide
Ligand	Manganese
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	defense response to bacterium Inferred from expression pattern. Source: TAIR photoinhibition Inferred from mutant phenotype. Source: TAIR photosystem II assembly Inferred from mutant phenotype Ref.11. Source: TAIR photosystem II stabilization Inferred from mutant phenotype Ref.11. Source: TAIR regulation of protein dephosphorylation Inferred from mutant phenotype. Source: TAIR
Cellular component	apoplast Inferred from direct assay. Source: TAIR chloroplast thylakoid lumen Inferred from direct assay. Source: TAIR chloroplast thylakoid membrane Inferred from direct assay. Source: TAIR extrinsic to membrane Inferred from electronic annotation. Source: InterPro oxygen evolving complex Inferred from electronic annotation. Source: InterPro plastoglobule Inferred from direct assay. Source: TAIR
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro oxygen evolving activity Inferred from direct assay. Source: TAIR poly(U) RNA binding Inferred from direct assay. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
trxA	P0AA25	2	EBI-449414,EBI-368542	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 58

Chloroplast

Transit peptide

59 – 85

Thylakoid

Chain

86 – 332

247

Oxygen-evolving enhancer protein 1-1, chloroplastic

PRO_0000029553

Experimental info

Sequence conflict

D → N in AAN15726. Ref.5

Sequence conflict

D → N in AAM96957. Ref.5

Sequence conflict

T → S in AAL08257. Ref.5

Sequence conflict

113 – 114

QC → HS in CAA36675. Ref.1

Sequence conflict

132

A → S in AAM65169. Ref.6

Sequence conflict

206

G → C in AAK96774. Ref.5

Sequence conflict

278

T → S in AAK49614. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P23321 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 73174DDC09C11E5C
Show »

FASTA

332

35,142

        10         20         30         40         50         60 
MAASLQSTAT FLQSAKIATA PSRGSSHLRS TQAVGKSFGL ETSSARLTCS FQSDFKDFTG 

        70         80         90        100        110        120 
KCSDAVKIAG FALATSALVV SGASAEGAPK RLTYDEIQSK TYMEVKGTGT ANQCPTIDGG 

       130        140        150        160        170        180 
SETFSFKPGK YAGKKFCFEP TSFTVKADSV SKNAPPEFQN TKLMTRLTYT LDEIEGPFEV 

       190        200        210        220        230        240 
ASDGSVNFKE EDGIDYAAVT VQLPGGERVP FLFTVKQLDA SGKPDSFTGK FLVPSYRGSS 

       250        260        270        280        290        300 
FLDPKGRGGS TGYDNAVALP AGGRGDEEEL VKENVKNTAA SVGEITLKVT KSKPETGEVI 

       310        320        330 
GVFESLQPSD TDLGAKVPKD VKIQGVWYGQ LE

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of cDNAs from Lycopersicon esculentum and Arabidopsis thaliana encoding the 33 kDa protein of the photosystem II-associated oxygen-evolving complex." Ko K., Granell A., Bennett J., Cashmore A.R. Plant Mol. Biol. 14:217-227(1990) [PubMed: 2101691] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The psbO gene for 33-kDa precursor polypeptide of the oxygen-evolving complex in Arabidopsis thaliana -- nucleotide sequence and control of its expression." Jain P.K., Kochhar A., Khurana J.P., Tyagi A.K. DNA Res. 5:221-228(1998) [PubMed: 9802567] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence features of the regions of 1,367,185 bp covered by 19 physically assigned P1 and TAC clones." Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N., Tabata S. DNA Res. 5:203-216(1998) [PubMed: 9734815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[7]	"An inventory of 1152 expressed sequence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana." Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M., Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D., Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M., Cooke R. Lescure B. Plant J. 4:1051-1061(1993) [PubMed: 8281187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 130-179. Strain: cv. C24. Tissue: Flower bud.
[8]	"Targeting of proteins to the thylakoid lumen by the bipartite transit peptide of the 33 kd oxygen-evolving protein." Ko K., Cashmore A.R. EMBO J. 8:3187-3194(1989) [PubMed: 2583096] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-90, SUBCELLULAR LOCATION.
[9]	"Central functions of the lumenal and peripheral thylakoid proteome of Arabidopsis determined by experimentation and genome-wide prediction." Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G., Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G., van Wijk K.J. Plant Cell 14:211-236(2002) [PubMed: 11826309] [Abstract] Cited for: PROTEIN SEQUENCE OF N-TERMINUS, IDENTIFICATION BY MASS SPECTROMETRY.
[10]	"Characterization of an Arabidopsis thaliana mutant with impaired psbO, one of two genes encoding extrinsic 33-kDa proteins in photosystem II." Murakami R., Ifuku K., Takabayashi A., Shikanai T., Endo T., Sato F. FEBS Lett. 523:138-142(2002) [PubMed: 12123820] [Abstract] Cited for: FUNCTION.
[11]	"The manganese-stabilizing protein is required for photosystem II assembly/stability and photoautotrophy in higher plants." Yi X., McChargue M., Laborde S., Frankel L.K., Bricker T.M. J. Biol. Chem. 280:16170-16174(2005) [PubMed: 15722336] [Abstract] Cited for: FUNCTION.
[12]	"Sorting signals, N-terminal modifications and abundance of the chloroplast proteome." Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J. PLoS ONE 3:E1994-E1994(2008) [PubMed: 18431481] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52428 mRNA. Translation: CAA36675.1. Y15433 Genomic DNA. Translation: CAA75629.1. AB013389 Genomic DNA. Translation: BAB10933.1. CP002688 Genomic DNA. Translation: AED98230.1. AF424626 mRNA. Translation: AAL11619.1. AY052299 mRNA. Translation: AAK96492.1. AY061924 mRNA. Translation: AAL31251.1. AF372898 mRNA. Translation: AAK49614.1. AY136291 mRNA. Translation: AAM96957.1. AY054583 mRNA. Translation: AAK96774.1. AY056401 mRNA. Translation: AAL08257.1. BT000407 mRNA. Translation: AAN15726.1. AY087630 mRNA. Translation: AAM65169.1. Z18374 mRNA. Translation: CAA79171.1. Frameshift.
IPI	IPI00545883.
PIR	S11852.
RefSeq	NP_201458.1. NM_126055.3.
UniGene	At.25361. At.48629. At.72732.
3D structure databases
ProteinModelPortal	P23321.
SMR	P23321. Positions 98-332.
ModBase	Search...
Protein-protein interaction databases
IntAct	P23321. 3 interactions.
MINT	MINT-2583944.
STRING	P23321.
2D gel databases
SWISS-2DPAGE	P23321.
Proteomic databases
PRIDE	P23321.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G66570.1; AT5G66570.1; AT5G66570.
GeneID	836789.
GenomeReviews	Gene locus AT5G66570 in contig BA000015_GR.
KEGG	ath:AT5G66570.
NMPDR	fig\|3702.1.peg.28761.
Organism-specific databases
TAIR	At5g66570.
Phylogenomic databases
eggNOG	NOG05777.
GeneTree	EPGT00050000017718.
InParanoid	P23321.
OMA	GKANDCP.
PhylomeDB	P23321.
ProtClustDB	PLN00037.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-1069.
Gene expression databases
Genevestigator	P23321.
GermOnline	AT5G66570. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002628. PSII_MSP. [Graphical view]
KO	K02716.
Pfam	PF01716. MSP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSBO1_ARATH

Accession

Primary (citable) accession number: P23321
Secondary accession number(s): O81917

Q94JV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	December 1, 2000
Last modified:	December 14, 2011
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents