P23316 (CHS1_CANAX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 1
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 1

Gene names

Name:

CHS1

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	776 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Formation and repair of the disk-shaped septum in yeast and the cross walls of the hyphal phase.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Enzyme regulation	Requires proteolytic activation.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Developmental stage	Yeast and hyphal stages, the latter having a higher specific activity.
Induction	Under certain adverse conditions.
Sequence similarities	Belongs to the chitin synthase family.
Caution	It is uncertain whether Met-1 or Met-58 is the initiator.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 776	776	Chitin synthase 1		PRO_0000193686
Regions
	Transmembrane	451 – 471	21	Helical; Potential
	Transmembrane	487 – 507	21	Helical; Potential
	Transmembrane	523 – 543	21	Helical; Potential
	Transmembrane	558 – 578	21	Helical; Potential
	Transmembrane	695 – 714	20	Helical; Potential
	Transmembrane	723 – 743	21	Helical; Potential
Experimental info
	Sequence conflict	446	1	Missing in BAA07738. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P23316 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: DDEBC5C754DE1597	FASTA	776	88,230
	10 20 30 40 50 60 MHNINNGYVP NREKTITKRK VRLVGGKAGN LVLENPVPTE LRKVLTRTES PFGEFTNMTY 70 80 90 100 110 120 TACTSQPDTF SAEGFTLRAA KYGRETEIVI CITMYNEDEV AFARTMHGVM KNIAHLCSRH 130 140 150 160 170 180 KSKIWGKDSW KKVQVIIVAD GRNKVQQSVL ELLTATGCYQ ENLARPYVNN SKVNAHLFEY 190 200 210 220 230 240 TTQISIDENL KFKGDEKNLA PVQVLFCLKE SNQKKINSHR WLFNAFCPVL DPNVIVLLDV 250 260 270 280 290 300 GTKPDNHAIY NLWKAFDRDS NVAGAAGEIK AMKGKGWINL TNPLVASQNF EYKLSNILDK 310 320 330 340 350 360 PLESLFGYIS VLPGALSAYR YIALKNHDDG TGPLASYFKG EDLLCSHDKD KENTKANFFE 370 380 390 400 410 420 ANMYLAEDRI LCWELVSKRN DNWVLKFVKS ATGETDVPET IAEFLSQRRR WINGAFFAAL 430 440 450 460 470 480 YSLYHFRKIW TTDHSYARKF WLHVEEFIYQ LVSLLFSFFS LSNFYLTFYF LTGSLVSYKS 490 500 510 520 530 540 LGKKGGFWIF TLFNYLCIGV LTSLFIVSIG NRPHASKNIF KTLIILLTIC ALYALVVGFV 550 560 570 580 590 600 FVINTIATFG TGGTSTYVLV SIVVSLLSTY GLYTLMSILY LDPWHMLTCS VQYFLMIPSY 610 620 630 640 650 660 TCTLQIFAFC NTHDVSWGTK GDNNPKEDLS NQYIIEKNAS GEFEAVIVDT NIDEDYLETL 670 680 690 700 710 720 YNIRSKRSNK KVALGHSEKT PLDGDDYAKD VRTRVVLFWM IANLVFIMTM VQVYEPGDTG 730 740 750 760 770 RNIYLAFILW AVAVLALVRA IGSLGYLIQT YARFFVESKS KWMKRGYTAP SHNPLN

References

[1]	"Isolation of a chitin synthase gene (CHS1) from Candida albicans by expression in Saccharomyces cerevisiae." Au-Young J., Robbins P.W. Mol. Microbiol. 4:197-207(1990) [PubMed: 2140148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 73/055.
[2]	"Isolation of canCHS1A, a variant gene of Candida albicans chitin synthase." Sudoh M., Watanabe M., Mio T., Nagahashi S., Yamada-Okabe H., Takagi M., Arisawa M. Microbiology 141:2673-2679(1995) [PubMed: 7582026] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10259 / CBS 5796 / DSM 5817 / JCM 2078 / NBRC 1060.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X52420 Genomic DNA. Translation: CAA36671.1. D43627 Genomic DNA. Translation: BAA07738.1.
3D structure databases
ProteinModelPortal	P23316.
ModBase	Search...
Protein-protein interaction databases
STRING	P23316.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR004835. Chitin_synth_fng. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS1_CANAX

Accession

Primary (citable) accession number: P23316

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1995
Last modified:	October 19, 2011
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program