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Protein

Nitrate reductase [NADH]

Gene

NIA

Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.

Catalytic activityi

Nitrite + NAD+ + H2O = nitrate + NADH.

Cofactori

Protein has several cofactor binding sites:
  • FADBy similarityNote: Binds 1 FAD per subunit.By similarity
  • hemeBy similarityNote: Binds 1 heme group per subunit.By similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi204MolybdenumBy similarity1
Metal bindingi586Iron (heme axial ligand)PROSITE-ProRule annotation1
Metal bindingi609Iron (heme axial ligand)PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processNitrate assimilation
LigandFAD, Flavoprotein, Heme, Iron, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase [NADH] (EC:1.7.1.1)
Short name:
NR
Gene namesi
Name:NIA
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesChenopodiaceaeChenopodioideaeAnserineaeSpinacia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001660711 – 926Nitrate reductase [NADH]Add BLAST926

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi443InterchainSequence analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP23312

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP23312, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliP23312
SMRiP23312
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 626Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST76
Domaini670 – 782FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST113

Sequence similaritiesi

Belongs to the nitrate reductase family.Curated

Family and domain databases

Gene3Di3.10.120.10, 1 hit
3.90.420.10, 1 hit
InterProiView protein in InterPro
IPR001199 Cyt_B5-like_heme/steroid-bd
IPR036400 Cyt_B5-like_heme/steroid_sf
IPR018506 Cyt_B5_heme-BS
IPR017927 Fd_Rdtase_FAD-bd
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR014756 Ig_E-set
IPR005066 MoCF_OxRdtse_dimer
IPR008335 Mopterin_OxRdtase_euk
IPR001834 NADH-Cyt_B5_reductase
IPR012137 Nitr_rd_NADH
IPR008333 OxRdtase_FAD-bd_dom
IPR001433 OxRdtase_FAD/NAD-bd
IPR000572 OxRdtase_Mopterin-bd_dom
IPR036374 OxRdtase_Mopterin-bd_sf
IPR022407 OxRdtase_Mopterin_BS
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00173 Cyt-b5, 1 hit
PF00970 FAD_binding_6, 1 hit
PF03404 Mo-co_dimer, 1 hit
PF00175 NAD_binding_1, 1 hit
PF00174 Oxidored_molyb, 1 hit
PIRSFiPIRSF000233 Nitr_rd_NADH, 1 hit
PRINTSiPR00406 CYTB5RDTASE
PR00363 CYTOCHROMEB5
PR00407 EUMOPTERIN
PR00371 FPNCR
SMARTiView protein in SMART
SM01117 Cyt-b5, 1 hit
SUPFAMiSSF55856 SSF55856, 1 hit
SSF56524 SSF56524, 1 hit
SSF63380 SSF63380, 1 hit
SSF81296 SSF81296, 1 hit
PROSITEiView protein in PROSITE
PS00191 CYTOCHROME_B5_1, 1 hit
PS50255 CYTOCHROME_B5_2, 1 hit
PS51384 FAD_FR, 1 hit
PS00559 MOLYBDOPTERIN_EUK, 1 hit

Sequencei

Sequence statusi: Complete.

P23312-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASVDRQYH PAPMSGVVRT PFSNHHRSDS PVRNGYTFSN PPSSNGVVKP
60 70 80 90 100
GEKIKLVDNN SNSNNGSNNN NNRYDSDSEE DDDENEMNVW NEMIKKGNSE
110 120 130 140 150
LEPSSVDSRD EGTADQWIER NPSMIRLTGK HPFNSEPPLT RLMHHGFLTP
160 170 180 190 200
VPLHYVRNHG PVPNAKWEDW TVEVTGLVKR PIRFTMDQLV NDFQSREFPV
210 220 230 240 250
TLVCAGNRRK EQNMTKQSIG FNWGSAAVST SVWRGVPLRD VLKRCGVMSS
260 270 280 290 300
LKGALNVCFE GAEDLPGGGG SKYGTSVKRE FAMDPARDII LAYMQNGEKL
310 320 330 340 350
SPDHGYPVRM IIPGFIGGRM VKWLKRIIVT TTESDNYYHY KDNRVLPSHV
360 370 380 390 400
DAELANSEAW WYKQEYIINE LNVNSVITSP CHEEILPINA WTTQRPYTMR
410 420 430 440 450
GYAYSGGGRK VTRVEVTMDG GDTWDICELD HQERGSKYGK FWCWCFWSLE
460 470 480 490 500
VEVLDLLGAK EIGVRAWDES LNTQPEKLIW NVMGMMNNCW FRVKTNVCKP
510 520 530 540 550
HKGEIGIVFE HPTQPGNKSG GWMARERHLE ISDSGPTLKR TASTPFMNTT
560 570 580 590 600
SKMYSMSEVK KHNTADSAWI VVHGNVYNAT RFLKDHPGGS DSILINAGTD
610 620 630 640 650
CTEEFDAIHS DKAKRLLEDF RIGELISTGY TSDSSSPGNS VHGGSVYSGL
660 670 680 690 700
AGLAPITEAV PLRNVALNPR VKIPCKLIEK VSLSHDVRRF RFGLPSEDQV
710 720 730 740 750
LGLPVGKHIF LCANVDDKLC MRAYTPSSTI DVVGYFDLVV KVYFKDVHPR
760 770 780 790 800
FPNGGVMSQH LDSLSLGSIV DVKGPLGHIE YLGKGNFTVH GKPKFAKKLA
810 820 830 840 850
MISGGTGITP IYQVMQAILK DPEDKTEMHV VYANRTEEDI LLREELDKWA
860 870 880 890 900
DEFRDRVKVW YVVEKAEEGW KYDTGFISEK ILRDHVPAVG DDVLALTCGP
910 920
PPMIQFAVQP NLDKMGFDIK EQLLIF
Length:926
Mass (Da):103,971
Last modified:November 1, 1991 - v1
Checksum:iFF21DF01963F1AFC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti303D → A in BAA13047 (PubMed:9128133).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32600 mRNA Translation: AAA34033.1
D86226 Genomic DNA Translation: BAA13047.1
U08029 mRNA Translation: AAA18377.1
PIRiS11868 RDSPNH

Similar proteinsi

Entry informationi

Entry nameiNIA_SPIOL
AccessioniPrimary (citable) accession number: P23312
Secondary accession number(s): Q41377
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 23, 2018
This is version 103 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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