Reviewed, UniProtKB/Swiss-Prot P23307 (DHPH_BACSH)

Names and origin

Protein names

Recommended name:
    Phenylalanine dehydrogenase
      Short name=PheDH
    EC=1.4.1.20

Gene names

Name:

pdh

Organism

Bacillus sphaericus

Taxonomic identifier

1421 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Planococcaceae › Lysinibacillus

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH.
Pathway	Amino-acid biosynthesis; L-phenylalanine biosynthesis; L-phenylalanine from phenylpyruvate (PDH route): step 1/1.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro phenylalanine dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	Phenylalanine dehydrogenase		PRO_0000182807
Regions
	Nucleotide binding	191 – 197	7	NAD Potential
Sites
	Active site	91	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23307-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: D32450CE397ACA67	FASTA	381	41,578
	10 20 30 40 50 60 MAKQLEKSSK IGNEDVFQKI ANHEQIVFCN DPVSGLQAII AIHDTTLGPA LGGTRMYPYK 70 80 90 100 110 120 NVDEALEDVL RLSEGMTYKC AAADIDFGGG KAVIIGDPEK DKSPALFRAF GQFVESLNGR 130 140 150 160 170 180 FYTGTDMGTT MDDFVHAQKE TNFINGIPEQ YGGSGDSSIP TAQGVIYALK ATNQYLFGSD 190 200 210 220 230 240 SLSGKTYAIQ GLGKVGYKVA EQLLKAGADL FVTDIHENVL NSIKQKSEEL GGSVTIVKSD 250 260 270 280 290 300 DIYSVQADIF VPCAMGGIIN DKTIPKLKVK AVVGSANNQL KDLRHANVLN EKGILYAPDY 310 320 330 340 350 360 IVNAGGLIQV ADELYGPNKE RVLLKTKEIY RSLLEIFNQA ALDCITTVEA ANRKCQKTIE 370 380 GQQTRNSFFS RGRRPKWNIK E

References

[1]	"Cloning and nucleotide sequencing of phenylalanine dehydrogenase gene of Bacillus sphaericus." Okazaki N., Hibino Y., Asano Y., Ohmori M., Numao N., Kondo K. Gene 63:337-341(1988) [PubMed: 2838396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M26661 Genomic DNA. Translation: AAA22646.1.
PIR	I39939.
3D structure databases
HSSP	HSSP built from PDB template 1C1D based on UniProtKB Q59771.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.4.1.20. 327.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer. IPR016211. Glu/Phe/Leu/Val_DH_bac/arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000188. Phe_leu_dh. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHPH_BACSH

Accession

Primary (citable) accession number: P23307

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	September 22, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)