P23299 (KCNE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily E member 1

Alternative name(s):
Delayed rectifier potassium channel subunit IsK
Short name=mISK
IKs producing slow voltage-gated potassium channel subunit beta Mink
Minimal potassium channel

Gene names

Name:

Kcne1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	129 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr) By similarity.
Subunit structure	Associates with KCNQ1/KVLQT1 and KCNH2/HERG By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Restrictively localized in the apical membrane portion of epithelial cells.
Post-translational modification	Phosphorylation inhibits the potassium current By similarity. N-glycosylation at Asn-26 occurs post-translationally, and requires prior cotranslational glycosylation at Asn-5 By similarity.
Sequence similarities	Belongs to the potassium channel KCNE family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	epithelial cell maturation Inferred from mutant phenotype PubMed 11223304. Source: MGI heart contraction Inferred from mutant phenotype PubMed 14561835. Source: MGI membrane depolarization Inferred from electronic annotation. Source: Compara positive regulation of potassium ion transmembrane transport Inferred from electronic annotation. Source: Compara potassium ion export Inferred from electronic annotation. Source: Compara protein N-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB protein O-linked glycosylation Inferred from sequence or structural similarity. Source: UniProtKB regulation of heart rate by cardiac conduction Inferred from mutant phenotype PubMed 14561835. Source: MGI
Cellular_component	Z disc Inferred from electronic annotation. Source: Compara apical plasma membrane Inferred from direct assay PubMed 11223304. Source: MGI lysosome Inferred from electronic annotation. Source: Compara voltage-gated potassium channel complex Inferred from electronic annotation. Source: Compara
Molecular_function	delayed rectifier potassium channel activity Inferred from electronic annotation. Source: Compara potassium channel regulator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 129	129	Potassium voltage-gated channel subfamily E member 1		PRO_0000144279
Regions
	Transmembrane	44 – 66	23	Helical; Potential
	Topological domain	67 – 129	63	Cytoplasmic Potential
Amino acid modifications
	Modified residue	102	1	Phosphoserine; by PKC By similarity
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Glycosylation	7	1	O-linked (GalNAc...) By similarity
	Glycosylation	26	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P23299 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: E66DF4742300E839	FASTA	129	14,578
	10 20 30 40 50 60 MSLPNSTTVL PFLARLWQET AEQGGNVSGL ARKSQLRDDS KLEALYILMV LGFFGFFTLG 70 80 90 100 110 120 IMLSYIRSKK LEHSHDPFNV YIESDAWQEK GKAVFQARVL ESFRACYVIE NQAAVEQPAT HLPELKPLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, pharmacology and regulation of a delayed rectifier K+ channel in mouse heart." Honore E., Attali B., Romey G., Heurteaux C., Ricard P., Lesage F., Lazdunski M., Barhanin J. EMBO J. 10:2805-2811(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Heart.
[2]	"ISK, a slowly activating voltage-sensitive K+ channel. Characterization of multiple cDNAs and gene organization in the mouse." Lesage F., Attali B., Lazdunski M., Barhanin J. FEBS Lett. 301:168-172(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Skin.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60457 mRNA. Translation: CAA42990.1. AK028907 mRNA. Translation: BAC26189.1.
IPI	IPI00130492.
PIR	S17307.
RefSeq	NP_032450.1. NM_008424.3.
UniGene	Mm.299425.
3D structure databases
ProteinModelPortal	P23299.
SMR	P23299. Positions 1-127.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000052248.
PTM databases
PhosphoSite	P23299.
Proteomic databases
PRIDE	P23299.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000051705; ENSMUSP00000052248; ENSMUSG00000039639. ENSMUST00000166707; ENSMUSP00000130866; ENSMUSG00000039639.
GeneID	16509.
KEGG	mmu:16509.
Organism-specific databases
CTD	3753.
MGI	MGI:96673. Kcne1.
Phylogenomic databases
eggNOG	NOG41824.
HOGENOM	HOG000113207.
HOVERGEN	HBG052226.
InParanoid	P23299.
KO	K04894.
OMA	ALYILMV.
OrthoDB	EOG41G35J.
Gene expression databases
ArrayExpress	P23299.
Bgee	P23299.
Genevestigator	P23299.
GermOnline	ENSMUSG00000039639. Mus musculus.
Family and domain databases
InterPro	IPR000369. K_chnl_volt-dep_bsu_KCNE. IPR005424. K_chnl_volt-dep_bsu_KCNE1. [Graphical view]
PANTHER	PTHR17028. PTHR17028. 1 hit.
Pfam	PF02060. ISK_Channel. 1 hit. [Graphical view]
PRINTS	PR01604. KCNE1CHANNEL. PR00168. KCNECHANNEL.
ProtoNet	Search...
Other
NextBio	289843.
SOURCE	Search...

Entry information

Entry name

KCNE1_MOUSE

Accession

Primary (citable) accession number: P23299

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program