Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium voltage-gated channel subfamily E member 1

Gene

Kcne1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr) (By similarity).By similarity

GO - Molecular functioni

  1. delayed rectifier potassium channel activity Source: Ensembl
  2. potassium channel regulator activity Source: GO_Central

GO - Biological processi

  1. cellular response to acidic pH Source: Ensembl
  2. epithelial cell maturation Source: MGI
  3. heart contraction Source: MGI
  4. male gonad development Source: Ensembl
  5. membrane repolarization during action potential Source: GO_Central
  6. negative regulation of protein targeting to membrane Source: Ensembl
  7. positive regulation of potassium ion transmembrane transport Source: Ensembl
  8. protein N-linked glycosylation Source: UniProtKB
  9. protein O-linked glycosylation Source: UniProtKB
  10. regulation of heart rate by cardiac conduction Source: MGI
  11. regulation of membrane repolarization Source: GO_Central
  12. regulation of potassium ion transmembrane transport Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium voltage-gated channel subfamily E member 1
Alternative name(s):
Delayed rectifier potassium channel subunit IsK
Short name:
mISK
IKs producing slow voltage-gated potassium channel subunit beta Mink
Minimal potassium channel
Gene namesi
Name:Kcne1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:96673. Kcne1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei44 – 6623HelicalSequence AnalysisAdd
BLAST
Topological domaini67 – 12963CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: MGI
  2. endoplasmic reticulum Source: Ensembl
  3. Golgi apparatus Source: Ensembl
  4. voltage-gated potassium channel complex Source: GO_Central
  5. Z disc Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 129129Potassium voltage-gated channel subfamily E member 1PRO_0000144279Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence Analysis
Glycosylationi7 – 71O-linked (GalNAc...)By similarity
Glycosylationi26 – 261N-linked (GlcNAc...)Sequence Analysis
Modified residuei102 – 1021Phosphoserine; by PKCBy similarity

Post-translational modificationi

Phosphorylation inhibits the potassium current.By similarity
N-glycosylation at Asn-26 occurs post-translationally, and requires prior cotranslational glycosylation at Asn-5.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP23299.

PTM databases

PhosphoSiteiP23299.

Expressioni

Tissue specificityi

Restrictively localized in the apical membrane portion of epithelial cells.

Gene expression databases

BgeeiP23299.
ExpressionAtlasiP23299. baseline and differential.
GenevestigatoriP23299.

Interactioni

Subunit structurei

Associates with KCNQ1/KVLQT1 and KCNH2/HERG.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052248.

Structurei

3D structure databases

ProteinModelPortaliP23299.
SMRiP23299. Positions 1-127.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the potassium channel KCNE family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG41824.
HOGENOMiHOG000113207.
HOVERGENiHBG052226.
InParanoidiP23299.
KOiK04894.
OMAiALYILMV.
OrthoDBiEOG7WMCMH.
PhylomeDBiP23299.
TreeFamiTF335976.

Family and domain databases

InterProiIPR000369. K_chnl_volt-dep_bsu_KCNE.
IPR005424. K_chnl_volt-dep_bsu_KCNE1.
[Graphical view]
PANTHERiPTHR17028. PTHR17028. 1 hit.
PfamiPF02060. ISK_Channel. 1 hit.
[Graphical view]
PRINTSiPR01604. KCNE1CHANNEL.
PR00168. KCNECHANNEL.

Sequencei

Sequence statusi: Complete.

P23299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLPNSTTVL PFLARLWQET AEQGGNVSGL ARKSQLRDDS KLEALYILMV
60 70 80 90 100
LGFFGFFTLG IMLSYIRSKK LEHSHDPFNV YIESDAWQEK GKAVFQARVL
110 120
ESFRACYVIE NQAAVEQPAT HLPELKPLS
Length:129
Mass (Da):14,578
Last modified:November 1, 1991 - v1
Checksum:iE66DF4742300E839
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60457 mRNA. Translation: CAA42990.1.
AK028907 mRNA. Translation: BAC26189.1.
CCDSiCCDS28336.1.
PIRiS17307.
RefSeqiNP_032450.1. NM_008424.3.
XP_006523004.1. XM_006522941.2.
UniGeneiMm.299425.

Genome annotation databases

EnsembliENSMUST00000051705; ENSMUSP00000052248; ENSMUSG00000039639.
ENSMUST00000166707; ENSMUSP00000130866; ENSMUSG00000039639.
GeneIDi16509.
KEGGimmu:16509.
UCSCiuc007zza.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60457 mRNA. Translation: CAA42990.1.
AK028907 mRNA. Translation: BAC26189.1.
CCDSiCCDS28336.1.
PIRiS17307.
RefSeqiNP_032450.1. NM_008424.3.
XP_006523004.1. XM_006522941.2.
UniGeneiMm.299425.

3D structure databases

ProteinModelPortaliP23299.
SMRiP23299. Positions 1-127.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000052248.

PTM databases

PhosphoSiteiP23299.

Proteomic databases

PRIDEiP23299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000051705; ENSMUSP00000052248; ENSMUSG00000039639.
ENSMUST00000166707; ENSMUSP00000130866; ENSMUSG00000039639.
GeneIDi16509.
KEGGimmu:16509.
UCSCiuc007zza.1. mouse.

Organism-specific databases

CTDi3753.
MGIiMGI:96673. Kcne1.

Phylogenomic databases

eggNOGiNOG41824.
HOGENOMiHOG000113207.
HOVERGENiHBG052226.
InParanoidiP23299.
KOiK04894.
OMAiALYILMV.
OrthoDBiEOG7WMCMH.
PhylomeDBiP23299.
TreeFamiTF335976.

Miscellaneous databases

NextBioi289843.
PROiP23299.
SOURCEiSearch...

Gene expression databases

BgeeiP23299.
ExpressionAtlasiP23299. baseline and differential.
GenevestigatoriP23299.

Family and domain databases

InterProiIPR000369. K_chnl_volt-dep_bsu_KCNE.
IPR005424. K_chnl_volt-dep_bsu_KCNE1.
[Graphical view]
PANTHERiPTHR17028. PTHR17028. 1 hit.
PfamiPF02060. ISK_Channel. 1 hit.
[Graphical view]
PRINTSiPR01604. KCNE1CHANNEL.
PR00168. KCNECHANNEL.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression, pharmacology and regulation of a delayed rectifier K+ channel in mouse heart."
    Honore E., Attali B., Romey G., Heurteaux C., Ricard P., Lesage F., Lazdunski M., Barhanin J.
    EMBO J. 10:2805-2811(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Heart.
  2. "ISK, a slowly activating voltage-sensitive K+ channel. Characterization of multiple cDNAs and gene organization in the mouse."
    Lesage F., Attali B., Lazdunski M., Barhanin J.
    FEBS Lett. 301:168-172(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin.

Entry informationi

Entry nameiKCNE1_MOUSE
AccessioniPrimary (citable) accession number: P23299
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 1, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.