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P23299 (KCNE1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium voltage-gated channel subfamily E member 1
Alternative name(s):
Delayed rectifier potassium channel subunit IsK
Short name=mISK
IKs producing slow voltage-gated potassium channel subunit beta Mink
Minimal potassium channel
Gene names
Name:Kcne1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length129 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ancillary protein that assembles as a beta subunit with a voltage-gated potassium channel complex of pore-forming alpha subunits. Modulates the gating kinetics and enhances stability of the channel complex. Assembled with KCNQ1/KVLQT1 is proposed to form the slowly activating delayed rectifier cardiac potassium (IKs) channel. The outward current reaches its steady state only after 50 seconds. Assembled with KCNH2/HERG may modulate the rapidly activating component of the delayed rectifying potassium current in heart (IKr) By similarity.

Subunit structure

Associates with KCNQ1/KVLQT1 and KCNH2/HERG By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Restrictively localized in the apical membrane portion of epithelial cells.

Post-translational modification

Phosphorylation inhibits the potassium current By similarity.

N-glycosylation at Asn-26 occurs post-translationally, and requires prior cotranslational glycosylation at Asn-5 By similarity.

Sequence similarities

Belongs to the potassium channel KCNE family.

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   Molecular functionIon channel
Potassium channel
Voltage-gated channel
   PTMGlycoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcellular response to acidity

Inferred from electronic annotation. Source: Ensembl

cellular response to cAMP

Inferred from electronic annotation. Source: Ensembl

epithelial cell maturation

Inferred from mutant phenotype PubMed 11223304. Source: MGI

heart contraction

Inferred from mutant phenotype PubMed 14561835. Source: MGI

male gonad development

Inferred from electronic annotation. Source: Ensembl

negative regulation of protein targeting to membrane

Inferred from electronic annotation. Source: Ensembl

positive regulation of potassium ion transmembrane transport

Inferred from electronic annotation. Source: Ensembl

potassium ion export

Inferred from electronic annotation. Source: Ensembl

protein N-linked glycosylation

Inferred from sequence or structural similarity. Source: UniProtKB

protein O-linked glycosylation

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of delayed rectifier potassium channel activity

Inferred from electronic annotation. Source: Ensembl

regulation of heart rate by cardiac conduction

Inferred from mutant phenotype PubMed 14561835. Source: MGI

regulation of ventricular cardiac muscle cell membrane repolarization

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentZ disc

Inferred from electronic annotation. Source: Ensembl

apical plasma membrane

Inferred from direct assay PubMed 11223304. Source: MGI

cell surface

Inferred from electronic annotation. Source: Ensembl

endoplasmic reticulum

Inferred from electronic annotation. Source: Ensembl

lysosome

Inferred from electronic annotation. Source: Ensembl

voltage-gated potassium channel complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functiondelayed rectifier potassium channel activity

Inferred from electronic annotation. Source: Ensembl

potassium channel regulator activity

Inferred from electronic annotation. Source: Ensembl

voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 129129Potassium voltage-gated channel subfamily E member 1
PRO_0000144279

Regions

Transmembrane44 – 6623Helical; Potential
Topological domain67 – 12963Cytoplasmic Potential

Amino acid modifications

Modified residue1021Phosphoserine; by PKC By similarity
Glycosylation51N-linked (GlcNAc...) Potential
Glycosylation71O-linked (GalNAc...) By similarity
Glycosylation261N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P23299 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: E66DF4742300E839

FASTA12914,578
        10         20         30         40         50         60 
MSLPNSTTVL PFLARLWQET AEQGGNVSGL ARKSQLRDDS KLEALYILMV LGFFGFFTLG 

        70         80         90        100        110        120 
IMLSYIRSKK LEHSHDPFNV YIESDAWQEK GKAVFQARVL ESFRACYVIE NQAAVEQPAT 


HLPELKPLS 

« Hide

References

« Hide 'large scale' references
[1]"Cloning, expression, pharmacology and regulation of a delayed rectifier K+ channel in mouse heart."
Honore E., Attali B., Romey G., Heurteaux C., Ricard P., Lesage F., Lazdunski M., Barhanin J.
EMBO J. 10:2805-2811(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Heart.
[2]"ISK, a slowly activating voltage-sensitive K+ channel. Characterization of multiple cDNAs and gene organization in the mouse."
Lesage F., Attali B., Lazdunski M., Barhanin J.
FEBS Lett. 301:168-172(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Skin.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60457 mRNA. Translation: CAA42990.1.
AK028907 mRNA. Translation: BAC26189.1.
CCDSCCDS28336.1.
PIRS17307.
RefSeqNP_032450.1. NM_008424.3.
XP_006523004.1. XM_006522941.1.
UniGeneMm.299425.

3D structure databases

ProteinModelPortalP23299.
SMRP23299. Positions 1-127.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000052248.

PTM databases

PhosphoSiteP23299.

Proteomic databases

PRIDEP23299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000051705; ENSMUSP00000052248; ENSMUSG00000039639.
ENSMUST00000166707; ENSMUSP00000130866; ENSMUSG00000039639.
GeneID16509.
KEGGmmu:16509.
UCSCuc007zza.1. mouse.

Organism-specific databases

CTD3753.
MGIMGI:96673. Kcne1.

Phylogenomic databases

eggNOGNOG41824.
HOGENOMHOG000113207.
HOVERGENHBG052226.
InParanoidP23299.
KOK04894.
OMAIERPAND.
OrthoDBEOG7WMCMH.
PhylomeDBP23299.
TreeFamTF335976.

Gene expression databases

BgeeP23299.
GenevestigatorP23299.

Family and domain databases

InterProIPR000369. K_chnl_volt-dep_bsu_KCNE.
IPR005424. K_chnl_volt-dep_bsu_KCNE1.
[Graphical view]
PANTHERPTHR17028. PTHR17028. 1 hit.
PfamPF02060. ISK_Channel. 1 hit.
[Graphical view]
PRINTSPR01604. KCNE1CHANNEL.
PR00168. KCNECHANNEL.
ProtoNetSearch...

Other

NextBio289843.
PROP23299.
SOURCESearch...

Entry information

Entry nameKCNE1_MOUSE
AccessionPrimary (citable) accession number: P23299
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 9, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot