P23297 (S10A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A1

Alternative name(s):
S-100 protein alpha chain
S-100 protein subunit alpha
S100 calcium-binding protein A1

Gene names

Name:	S100A1
Synonyms:	S100A

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Subunit structure	Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Also forms heterodimers with S100P. Interacts with AGER and CAPZA1 By similarity. Interacts with FKBP4. Interacts with RYR1 and RYR2. Interacts with CACYBP in a calcium-dependent manner. Ref.8 Ref.9 Ref.10 Ref.11 Ref.13
Subcellular location	Cytoplasm.
Tissue specificity	Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	Calcium Metal-binding Zinc
PTM	S-nitrosylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Non-traceable author statement PubMed 12804600. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara regulation of heart contraction Non-traceable author statement PubMed 12804600. Source: UniProtKB
Cellular_component	nucleus Inferred from direct assay PubMed 12118070. Source: UniProtKB protein complex Non-traceable author statement PubMed 14638689. Source: UniProtKB sarcoplasmic reticulum Inferred from direct assay PubMed 12804600. Source: UniProtKB
Molecular_function	calcium ion binding Traceable author statement PubMed 1998503. Source: ProtInc protein homodimerization activity Inferred from direct assay PubMed 10913138. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		5	EBI-743686,EBI-743686
S100B	P04271	5	EBI-743686,EBI-458391
S100P	P25815	7	EBI-743686,EBI-743700

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 94

93

Protein S100-A1

PRO_0000143961

Regions

Domain

13 – 48

36

EF-hand 1

Domain

50 – 85

36

EF-hand 2

Calcium binding

20 – 33

14

1; low affinity

Calcium binding

63 – 74

12

2; high affinity

Amino acid modifications

Modified residue

86

1

S-nitrosocysteine Ref.14

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

94

Helix Strand Turn

Details...

Helix

4 – 20

17

Beta strand

22 – 24

3

Beta strand

26 – 30

5

Helix

31 – 41

11

Helix

45 – 48

4

Helix

52 – 64

13

Turn

65 – 67

3

Beta strand

68 – 70

3

Helix

72 – 87

16

Turn

88 – 90

3

Sequences

	Sequence		Length	Mass (Da)
	P23297 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AD5E53AF326B25D2	FASTA	94	10,546
	10 20 30 40 50 60 MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK DVDAVDKVMK 70 80 90 ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"S100 alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart." Engelkamp D., Schaefer B.W., Erne P., Heizmann C.W. Biochemistry 31:10258-10264(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Caudate nucleus.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Human protein factory for converting the transcriptome into an in vitro-expressed proteome." Goshima N., Kawamura Y., Fukumoto A., Miura A., Honma R., Satoh R., Wakamatsu A., Yamamoto J., Kimura K., Nishikawa T., Andoh T., Iida Y., Ishikawa K., Ito E., Kagawa N., Kaminaga C., Kanehori K., Kawakami B. , Kenmochi K., Kimura R., Kobayashi M., Kuroita T., Kuwayama H., Maruyama Y., Matsuo K., Minami K., Mitsubori M., Mori M., Morishita R., Murase A., Nishikawa A., Nishikawa S., Okamoto T., Sakagami N., Sakamoto Y., Sasaki Y., Seki T., Sono S., Sugiyama A., Sumiya T., Takayama T., Takayama Y., Takeda H., Togashi T., Yahata K., Yamada H., Yanagisawa Y., Endo Y., Imamoto F., Kisu Y., Tanaka S., Isogai T., Imai J., Watanabe S., Nomura N. Nat. Methods 5:1011-1017(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[8]	"CacyBP/SIP, a calcyclin and Siah-1-interacting protein, binds EF-hand proteins of the S100 family." Filipek A., Jastrzebska B., Nowotny M., Kuznicki J. J. Biol. Chem. 277:28848-28852(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CACYBP.
[9]	"Heterodimeric interaction and interfaces of S100A1 and S100P." Wang G., Zhang S., Fernig D.G., Spiller D., Martin-Fernandez M., Zhang H., Ding Y., Rao Z., Rudland P.S., Barraclough R. Biochem. J. 382:375-383(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH S100P.
[10]	"S100A1 and calmodulin compete for the same binding site on ryanodine receptor." Wright N.T., Prosser B.L., Varney K.M., Zimmer D.B., Schneider M.F., Weber D.J. J. Biol. Chem. 283:26676-26683(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RYR1 AND RYR2.
[11]	"S100 proteins regulate the interaction of Hsp90 with cyclophilin 40 and FKBP52 through their tetratricopeptide repeats." Shimamoto S., Kubota Y., Tokumitsu H., Kobayashi R. FEBS Lett. 584:1119-1125(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FKBP4.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]	"Solution NMR structure and dynamics of human apo-S100A1 protein." Nowakowski M., Jaremko L., Jaremko M., Zhukov I., Belczyk A., Bierzynski A., Ejchart A. J. Struct. Biol. 174:391-399(2011) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR, SUBUNIT.
[14]	"Post-translational S-nitrosylation is an endogenous factor fine tuning the properties of human S100A1 protein." Lenarcic Zivkovic M., Zareba-Koziol M., Zhukova L., Poznanski J., Zhukov I., Wyslouch-Cieszynska A. J. Biol. Chem. 287:40457-40470(2012) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR OF 2-94, S-NITROSYLATION AT CYS-86.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X58079 mRNA. Translation: CAA41107.1.
AK312152 mRNA. Translation: BAG35086.1.
BT006938 mRNA. Translation: AAP35584.1.
AB451316 mRNA. Translation: BAG70130.1.
AB451446 mRNA. Translation: BAG70260.1.
AL162258 Genomic DNA. Translation: CAI19677.1.
CH471121 Genomic DNA. Translation: EAW53302.1.
BC014392 mRNA. Translation: AAH14392.1.

IPI

IPI00645016.

PIR

BCHUIA. A44470.

RefSeq

NP_006262.1. NM_006271.1.

UniGene

Hs.515715.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L0P	NMR	-	A/B	1-94	[»]
2LHL	NMR	-	A/B	2-94	[»]
2LLS	NMR	-	A/B	2-94	[»]
2LLT	NMR	-	A/B	2-94	[»]
2LLU	NMR	-	A/B	2-94	[»]
2LP2	NMR	-	A/B	2-94	[»]
2LP3	NMR	-	A/B	2-94	[»]

ProteinModelPortal

P23297.

ModBase

Search...

Protein-protein interaction databases

IntAct

P23297. 6 interactions.

STRING

9606.ENSP00000292169.

Polymorphism databases

DMDM

134136.

Proteomic databases

PaxDb

P23297.

PRIDE

P23297.

Protocols and materials databases

DNASU

6271.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000292169; ENSP00000292169; ENSG00000160678.

GeneID

6271.

KEGG

hsa:6271.

UCSC

uc001fck.1. human.

Organism-specific databases

CTD

6271.

GeneCards

GC01P153600.

HGNC

HGNC:10486. S100A1.

HPA

CAB002599.
HPA006462.

MIM

176940. gene.

neXtProt

NX_P23297.

PharmGKB

PA34898.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG277258.

HOGENOM

HOG000246968.

HOVERGEN

HBG001479.

InParanoid

P23297.

OrthoDB

EOG4Z8XZ3.

PhylomeDB

P23297.

Gene expression databases

ArrayExpress

P23297.

Bgee

P23297.

CleanEx

HS_S100A1.

Genevestigator

P23297.

GermOnline

ENSG00000160678. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]

Pfam

PF00036. efhand. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00768. Olopatadine.

GenomeRNAi

6271.

NextBio

24339.

SOURCE

Search...

Entry information

Entry name

S10A1_HUMAN

Accession

Primary (citable) accession number: P23297
Secondary accession number(s): B2R5D9, Q5T7Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 130 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.