P23297 (S10A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein S100-A1

Alternative name(s):
S-100 protein alpha chain
S-100 protein subunit alpha
S100 calcium-binding protein A1

Gene names

Name:	S100A1
Synonyms:	S100A

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	94 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Subunit structure	Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. Interacts with AGER and CAPZA1 By similarity. Interacts with FKBP4. Ref.5 Ref.6
Subcellular location	Cytoplasm.
Tissue specificity	Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similarities	Belongs to the S-100 family. Contains 2 EF-hand domains.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Repeat
Ligand	Calcium Metal-binding Zinc
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular signal transduction Non-traceable author statement. Source: UniProtKB regulation of heart contraction Non-traceable author statement. Source: UniProtKB
Cellular component	nucleus Inferred from direct assay. Source: UniProtKB protein complex Non-traceable author statement. Source: UniProtKB sarcoplasmic reticulum Inferred from direct assay. Source: UniProtKB
Molecular function	ATPase binding Inferred from physical interaction. Source: UniProtKB S100 alpha binding Inferred from physical interaction. Source: UniProtKB S100 beta binding Inferred from physical interaction. Source: UniProtKB calcium ion binding Traceable author statement. Source: ProtInc protein homodimerization activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		5	EBI-743686,EBI-743686
S100B	P04271	5	EBI-743686,EBI-458391
S100P	P25815	7	EBI-743686,EBI-743700

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 94

93

Protein S100-A1

PRO_0000143961

Regions

Domain

13 – 48

36

EF-hand 1

Domain

50 – 85

36

EF-hand 2

Calcium binding

20 – 33

14

1; low affinity

Calcium binding

63 – 74

12

2; high affinity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

94

Helix Strand Turn

Details...

Helix

4 – 19

16

Helix

31 – 41

11

Turn

46 – 48

3

Helix

52 – 64

13

Helix

72 – 87

16

Turn

88 – 90

3

Sequences

	Sequence		Length	Mass (Da)
	P23297 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: AD5E53AF326B25D2	FASTA	94	10,546
	10 20 30 40 50 60 MGSELETAME TLINVFHAHS GKEGDKYKLS KKELKELLQT ELSGFLDAQK DVDAVDKVMK 70 80 90 ELDENGDGEV DFQEYVVLVA ALTVACNNFF WENS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"S100 alpha, CAPL, and CACY: molecular cloning and expression analysis of three calcium-binding proteins from human heart." Engelkamp D., Schaefer B.W., Erne P., Heizmann C.W. Biochemistry 31:10258-10264(1992) [PubMed: 1384693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[5]	"S100 proteins regulate the interaction of Hsp90 with Cyclophilin 40 and FKBP52 through their tetratricopeptide repeats." Shimamoto S., Kubota Y., Tokumitsu H., Kobayashi R. FEBS Lett. 584:1119-1125(2010) [PubMed: 20188096] [Abstract] Cited for: INTERACTION WITH FKBP4.
[6]	"Solution NMR structure and dynamics of human apo-S100A1 protein." Nowakowski M., Jaremko L., Jaremko M., Zhukov I., Belczyk A., Bierzynski A., Ejchart A. J. Struct. Biol. 174:391-399(2011) [PubMed: 21296671] [Abstract] Cited for: STRUCTURE BY NMR, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X58079 mRNA. Translation: CAA41107.1.
BT006938 mRNA. Translation: AAP35584.1.
AL162258 Genomic DNA. Translation: CAI19677.1.
BC014392 mRNA. Translation: AAH14392.1.

IPI

IPI00645016.

PIR

BCHUIA. A44470.

RefSeq

NP_006262.1. NM_006271.1.

UniGene

Hs.515715.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L0P	NMR	-	A/B	1-94	[»]

ProteinModelPortal

P23297.

SMR

P23297. Positions 2-94.

ModBase

Search...

Protein-protein interaction databases

IntAct

P23297. 7 interactions.

STRING

P23297.

Polymorphism databases

DMDM

134136.

Proteomic databases

PRIDE

P23297.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000292169; ENSP00000292169; ENSG00000160678.
ENST00000368698; ENSP00000357687; ENSG00000160678.

GeneID

6271.

KEGG

hsa:6271.

UCSC

uc001fck.1. human.

Organism-specific databases

CTD

6271.

GeneCards

GC01P153600.

H-InvDB

HIX0199970.

HGNC

HGNC:10486. S100A1.

HPA

CAB002599.
HPA006462.

MIM

176940. gene.

neXtProt

NX_P23297.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG20611.

HOVERGEN

HBG001479.

InParanoid

P23297.

OrthoDB

EOG4Z8XZ3.

PhylomeDB

P23297.

Gene expression databases

ArrayExpress

P23297.

Bgee

P23297.

CleanEx

HS_S100A1.

Genevestigator

P23297.

GermOnline

ENSG00000160678. Homo sapiens.

Family and domain databases

InterPro

IPR018248. EF-hand.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR001751. S100/CaBP-9k_CS.
IPR013787. S100_Ca-bd_sub.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

Pfam

PF00036. efhand. 1 hit.
PF01023. S_100. 1 hit.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
PS00303. S100_CABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00768. Olopatadine.

NextBio

24339.

SOURCE

Search...

Entry information

Entry name

S10A1_HUMAN

Accession

Primary (citable) accession number: P23297
Secondary accession number(s): Q5T7Y3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 116 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.