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P23293

- BUR1_YEAST

UniProt

P23293 - BUR1_YEAST

Protein

Serine/threonine-protein kinase BUR1

Gene

SGV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 152 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase component of the BUR kinase complex involved in transcription regulation. This complex phosphorylates 'Ser-120' of the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B, the localization of the PAF1 complex to the chromatin, and the silencing of telomeric-associated genes. Also required for histone H3 'Lys-4' trimethylation. May phosphorylate the 'Ser-5' of the RBP1 carboxy-terminal domain (CTD) repeats. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase. The kinase activity of the complex requires the presence of BUR2.8 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei89 – 891ATPPROSITE-ProRule annotations
    Active sitei195 – 1951Proton acceptorPROSITE-ProRule annotations

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi66 – 749ATPPROSITE-ProRule annotations

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: SGD
    3. protein binding Source: IntAct
    4. RNA polymerase II carboxy-terminal domain kinase activity Source: SGD

    GO - Biological processi

    1. phosphorylation of RNA polymerase II C-terminal domain Source: SGD
    2. positive regulation of histone H3-K4 methylation Source: SGD
    3. protein phosphorylation Source: SGD
    4. regulation of cell cycle Source: GOC
    5. transcription, DNA-templated Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-34290-MONOMER.
    BRENDAi2.7.11.22. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase BUR1 (EC:2.7.11.22, EC:2.7.11.23)
    Alternative name(s):
    Bypass UAS requirement protein 1
    Suppressor of GPA1-Vall50 mutation protein 1
    Gene namesi
    Name:SGV1
    Synonyms:BUR1
    Ordered Locus Names:YPR161C
    ORF Names:P9584.8
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

    Organism-specific databases

    CYGDiYPR161c.
    SGDiS000006365. SGV1.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cyclin-dependent protein kinase holoenzyme complex Source: SGD
    2. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi107 – 1071E → Q: Loss of kinase activity in vitro. 1 Publication
    Mutagenesisi213 – 2131D → N: Loss of kinase activity in vitro. 1 Publication
    Mutagenesisi240 – 2401T → A, D or E: No phosphorylation of SGV1. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Serine/threonine-protein kinase BUR1PRO_0000085687Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei240 – 2401Phosphothreonine; by CAK1 Publication
    Modified residuei400 – 4001Phosphoserine1 Publication
    Modified residuei405 – 4051Phosphothreonine1 Publication
    Modified residuei417 – 4171Phosphoserine3 Publications
    Modified residuei634 – 6341Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP23293.
    PaxDbiP23293.
    PeptideAtlasiP23293.
    PRIDEiP23293.

    Expressioni

    Gene expression databases

    GenevestigatoriP23293.

    Interactioni

    Subunit structurei

    Belongs to the BUR kinase complex composed of SGV1/BUR1 and BUR2. Interacts with BUR2 and RBP1.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUR2Q059496EBI-17078,EBI-30948

    Protein-protein interaction databases

    BioGridi36334. 135 interactions.
    DIPiDIP-5916N.
    IntActiP23293. 14 interactions.
    MINTiMINT-668965.
    STRINGi4932.YPR161C.

    Structurei

    3D structure databases

    ProteinModelPortaliP23293.
    SMRiP23293. Positions 27-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini60 – 366307Protein kinasePROSITE-ProRule annotationsAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotations

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000116084.
    HOGENOMiHOG000233024.
    KOiK15562.
    OMAiPRINLEM.
    OrthoDBiEOG7K3TWD.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Sequencei

    Sequence statusi: Complete.

    P23293-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDNGSPAVL PKTEFNKYKI GKVKSTPAIQ RDAKTNLTYI KLRKRSSEKV    50
    YGCTVFQNHY REDEKLGQGT FGEVYKGIHL ETQRQVAMKK IIVSVEKDLF 100
    PITAQREITI LKRLNHKNII KLIEMVYDHS PDITNAASSN LHKSFYMILP 150
    YMVADLSGVL HNPRINLEMC DIKNMMLQIL EGLNYIHCAK FMHRDIKTAN 200
    ILIDHNGVLK LADFGLARLY YGCPPNLKYP GGAGSGAKYT SVVVTRWYRA 250
    PELVLGDKQY TTAVDIWGVG CVFAEFFEKK PILQGKTDID QGHVIFKLLG 300
    TPTEEDWAVA RYLPGAELTT TNYKPTLRER FGKYLSETGL DFLGQLLALD 350
    PYKRLTAMSA KHHPWFKEDP LPSEKITLPT EESHEADIKR YKEEMHQSLS 400
    QRVPTAPRGH IVEKGESPVV KNLGAIPRGP KKDDASFLPP SKNVLAKPPP 450
    SKIRELHQNP RPYHVNSGYA KTAIPPPAAP AGVNRYGPNN SSRNNRFSGN 500
    STAPNNSRNP VNRFHPETNV SSKYNKVPLP LGPQSRYQGN SNESRYKNSP 550
    NDSRYHNPRY VNKPETNFNR QPQKYSRQES NAPINKNYNP SNGSRNMAGD 600
    HHQGSRPSHP QFPISPSQGQ HQLTSKPIEK KNGSFKDERA KPDESKEFQN 650
    SDIADLY 657
    Length:657
    Mass (Da):74,239
    Last modified:November 1, 1991 - v1
    Checksum:iCC9034A4B10A510E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90317 Genomic DNA. Translation: BAA14347.1.
    L33835 Genomic DNA. Translation: AAB59314.1.
    U28371 Genomic DNA. Translation: AAB68058.1.
    BK006949 Genomic DNA. Translation: DAA11578.1.
    PIRiA39526.
    RefSeqiNP_015487.1. NM_001184258.1.

    Genome annotation databases

    EnsemblFungiiYPR161C; YPR161C; YPR161C.
    GeneIDi856290.
    KEGGisce:YPR161C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D90317 Genomic DNA. Translation: BAA14347.1 .
    L33835 Genomic DNA. Translation: AAB59314.1 .
    U28371 Genomic DNA. Translation: AAB68058.1 .
    BK006949 Genomic DNA. Translation: DAA11578.1 .
    PIRi A39526.
    RefSeqi NP_015487.1. NM_001184258.1.

    3D structure databases

    ProteinModelPortali P23293.
    SMRi P23293. Positions 27-374.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 36334. 135 interactions.
    DIPi DIP-5916N.
    IntActi P23293. 14 interactions.
    MINTi MINT-668965.
    STRINGi 4932.YPR161C.

    Proteomic databases

    MaxQBi P23293.
    PaxDbi P23293.
    PeptideAtlasi P23293.
    PRIDEi P23293.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YPR161C ; YPR161C ; YPR161C .
    GeneIDi 856290.
    KEGGi sce:YPR161C.

    Organism-specific databases

    CYGDi YPR161c.
    SGDi S000006365. SGV1.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000116084.
    HOGENOMi HOG000233024.
    KOi K15562.
    OMAi PRINLEM.
    OrthoDBi EOG7K3TWD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-34290-MONOMER.
    BRENDAi 2.7.11.22. 984.

    Miscellaneous databases

    NextBioi 981629.
    PROi P23293.

    Gene expression databases

    Genevestigatori P23293.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "SGV1 encodes a CDC28/cdc2-related kinase required for a G alpha subunit-mediated adaptive response to pheromone in S. cerevisiae."
      Irie K., Nomoto S., Miyajima I., Matsumoto K.
      Cell 65:785-795(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 204508 / S288c.
    2. "DNA sequence analysis of a 10.4 kbp region on the right arm of yeast chromosome XVI positions GPH1 and SGV1 adjacent to KRE6, and identifies two novel tRNA genes."
      Roemer T.D., Fortin N., Bussey H.
      Yeast 10:1527-1530(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI."
      Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W., Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D., Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E., Churcher C.M.
      , Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H., DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N., Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K., Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M., Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A., Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S., Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D., Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S., Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B., Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A., Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R., Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A., Vo D.H., Hani J.
      Nature 387:103-105(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Mutations that suppress the deletion of an upstream activating sequence in yeast: involvement of a protein kinase and histone H3 in repressing transcription in vivo."
      Prelich G., Winston F.
      Genetics 135:665-676(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "BUR1 and BUR2 encode a divergent cyclin-dependent kinase-cyclin complex important for transcription in vivo."
      Yao S., Neiman A., Prelich G.
      Mol. Cell. Biol. 20:7080-7087(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BUR2, FUNCTION.
    7. "Phosphorylation of the RNA polymerase II carboxy-terminal domain by the Bur1 cyclin-dependent kinase."
      Murray S., Udupa R., Yao S., Hartzog G., Prelich G.
      Mol. Cell. Biol. 21:4089-4096(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RBP1, FUNCTION.
    8. "Activation of the Bur1-Bur2 cyclin-dependent kinase complex by Cak1."
      Yao S., Prelich G.
      Mol. Cell. Biol. 22:6750-6758(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-240, MUTAGENESIS OF THR-240, FUNCTION.
    9. "Bur1 kinase is required for efficient transcription elongation by RNA polymerase II."
      Keogh M.-C., Podolny V., Buratowski S.
      Mol. Cell. Biol. 23:7005-7018(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF GLU-107; ASP-213 AND THR-240.
    10. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    11. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    12. "BUR kinase selectively regulates H3 K4 trimethylation and H2B ubiquitylation through recruitment of the PAF elongation complex."
      Laribee R.N., Krogan N.J., Xiao T., Shibata Y., Hughes T.R., Greenblatt J.F., Strahl B.D.
      Curr. Biol. 15:1487-1493(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    13. "The Bur1/Bur2 complex is required for histone H2B monoubiquitination by Rad6/Bre1 and histone methylation by COMPASS."
      Wood A., Schneider J., Dover J., Johnston M., Shilatifard A.
      Mol. Cell 20:589-599(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PHOSPHORYLATION OF UBC2.
    14. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    15. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    16. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
      Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
      Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-400 AND THR-405, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    17. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417 AND SER-634, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    18. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-417, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBUR1_YEAST
    AccessioniPrimary (citable) accession number: P23293
    Secondary accession number(s): D6W4G2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 152 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 2070 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XVI
      Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

    External Data

    Dasty 3

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