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Protein

Serine/threonine-protein kinase BUR1

Gene

SGV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase component of the BUR kinase complex involved in transcription regulation. This complex phosphorylates 'Ser-120' of the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B, the localization of the PAF1 complex to the chromatin, and the silencing of telomeric-associated genes. Also required for histone H3 'Lys-4' trimethylation. May phosphorylate the 'Ser-5' of the RBP1 carboxy-terminal domain (CTD) repeats. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase. The kinase activity of the complex requires the presence of BUR2.8 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89ATPPROSITE-ProRule annotation1
Active sitei195Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 74ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • RNA polymerase II carboxy-terminal domain kinase activity Source: SGD

GO - Biological processi

  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • positive regulation of histone H3-K4 methylation Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • protein phosphorylation Source: SGD
  • transcription, DNA-templated Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34290-MONOMER.
BRENDAi2.7.11.22. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BUR1 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Bypass UAS requirement protein 1
Suppressor of GPA1-Vall50 mutation protein 1
Gene namesi
Name:SGV1
Synonyms:BUR1
Ordered Locus Names:YPR161C
ORF Names:P9584.8
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR161C.
SGDiS000006365. SGV1.

Subcellular locationi

GO - Cellular componenti

  • cyclin-dependent protein kinase holoenzyme complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107E → Q: Loss of kinase activity in vitro. 1 Publication1
Mutagenesisi213D → N: Loss of kinase activity in vitro. 1 Publication1
Mutagenesisi240T → A, D or E: No phosphorylation of SGV1. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856871 – 657Serine/threonine-protein kinase BUR1Add BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei240Phosphothreonine; by CAK1 Publication1
Modified residuei400PhosphoserineCombined sources1
Modified residuei405PhosphothreonineCombined sources1
Modified residuei417PhosphoserineCombined sources1
Modified residuei634PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23293.
PRIDEiP23293.

PTM databases

iPTMnetiP23293.

Interactioni

Subunit structurei

Belongs to the BUR kinase complex composed of SGV1/BUR1 and BUR2. Interacts with BUR2 and RBP1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUR2Q059496EBI-17078,EBI-30948

Protein-protein interaction databases

BioGridi36334. 136 interactors.
DIPiDIP-5916N.
IntActiP23293. 14 interactors.
MINTiMINT-668965.

Structurei

3D structure databases

ProteinModelPortaliP23293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 366Protein kinasePROSITE-ProRule annotationAdd BLAST307

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00840000131002.
HOGENOMiHOG000233024.
InParanoidiP23293.
KOiK15562.
OMAiPRINLEM.
OrthoDBiEOG092C2FL8.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNGSPAVL PKTEFNKYKI GKVKSTPAIQ RDAKTNLTYI KLRKRSSEKV
60 70 80 90 100
YGCTVFQNHY REDEKLGQGT FGEVYKGIHL ETQRQVAMKK IIVSVEKDLF
110 120 130 140 150
PITAQREITI LKRLNHKNII KLIEMVYDHS PDITNAASSN LHKSFYMILP
160 170 180 190 200
YMVADLSGVL HNPRINLEMC DIKNMMLQIL EGLNYIHCAK FMHRDIKTAN
210 220 230 240 250
ILIDHNGVLK LADFGLARLY YGCPPNLKYP GGAGSGAKYT SVVVTRWYRA
260 270 280 290 300
PELVLGDKQY TTAVDIWGVG CVFAEFFEKK PILQGKTDID QGHVIFKLLG
310 320 330 340 350
TPTEEDWAVA RYLPGAELTT TNYKPTLRER FGKYLSETGL DFLGQLLALD
360 370 380 390 400
PYKRLTAMSA KHHPWFKEDP LPSEKITLPT EESHEADIKR YKEEMHQSLS
410 420 430 440 450
QRVPTAPRGH IVEKGESPVV KNLGAIPRGP KKDDASFLPP SKNVLAKPPP
460 470 480 490 500
SKIRELHQNP RPYHVNSGYA KTAIPPPAAP AGVNRYGPNN SSRNNRFSGN
510 520 530 540 550
STAPNNSRNP VNRFHPETNV SSKYNKVPLP LGPQSRYQGN SNESRYKNSP
560 570 580 590 600
NDSRYHNPRY VNKPETNFNR QPQKYSRQES NAPINKNYNP SNGSRNMAGD
610 620 630 640 650
HHQGSRPSHP QFPISPSQGQ HQLTSKPIEK KNGSFKDERA KPDESKEFQN

SDIADLY
Length:657
Mass (Da):74,239
Last modified:November 1, 1991 - v1
Checksum:iCC9034A4B10A510E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90317 Genomic DNA. Translation: BAA14347.1.
L33835 Genomic DNA. Translation: AAB59314.1.
U28371 Genomic DNA. Translation: AAB68058.1.
BK006949 Genomic DNA. Translation: DAA11578.1.
PIRiA39526.
RefSeqiNP_015487.1. NM_001184258.1.

Genome annotation databases

EnsemblFungiiYPR161C; YPR161C; YPR161C.
GeneIDi856290.
KEGGisce:YPR161C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90317 Genomic DNA. Translation: BAA14347.1.
L33835 Genomic DNA. Translation: AAB59314.1.
U28371 Genomic DNA. Translation: AAB68058.1.
BK006949 Genomic DNA. Translation: DAA11578.1.
PIRiA39526.
RefSeqiNP_015487.1. NM_001184258.1.

3D structure databases

ProteinModelPortaliP23293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36334. 136 interactors.
DIPiDIP-5916N.
IntActiP23293. 14 interactors.
MINTiMINT-668965.

PTM databases

iPTMnetiP23293.

Proteomic databases

MaxQBiP23293.
PRIDEiP23293.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYPR161C; YPR161C; YPR161C.
GeneIDi856290.
KEGGisce:YPR161C.

Organism-specific databases

EuPathDBiFungiDB:YPR161C.
SGDiS000006365. SGV1.

Phylogenomic databases

GeneTreeiENSGT00840000131002.
HOGENOMiHOG000233024.
InParanoidiP23293.
KOiK15562.
OMAiPRINLEM.
OrthoDBiEOG092C2FL8.

Enzyme and pathway databases

BioCyciYEAST:G3O-34290-MONOMER.
BRENDAi2.7.11.22. 984.

Miscellaneous databases

PROiP23293.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBUR1_YEAST
AccessioniPrimary (citable) accession number: P23293
Secondary accession number(s): D6W4G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 2, 2016
This is version 171 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XVI
    Yeast (Saccharomyces cerevisiae) chromosome XVI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.