Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein kinase BUR1

Gene

SGV1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase component of the BUR kinase complex involved in transcription regulation. This complex phosphorylates 'Ser-120' of the UBC2/RAD6 ubiquitin-conjugating enzyme (E2), leading to monoubiquitination of histone H2B, the localization of the PAF1 complex to the chromatin, and the silencing of telomeric-associated genes. Also required for histone H3 'Lys-4' trimethylation. May phosphorylate the 'Ser-5' of the RBP1 carboxy-terminal domain (CTD) repeats. Necessary for the recovery from pheromone-induced growth arrest in the cell cycle G1 phase. The kinase activity of the complex requires the presence of BUR2.8 Publications

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei89ATPPROSITE-ProRule annotation1
Active sitei195Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi66 – 74ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cyclin-dependent protein serine/threonine kinase activity Source: SGD
  • RNA polymerase II carboxy-terminal domain kinase activity Source: SGD

GO - Biological processi

  • phosphorylation of RNA polymerase II C-terminal domain Source: SGD
  • positive regulation of histone H3-K4 methylation Source: SGD
  • positive regulation of transcription elongation from RNA polymerase II promoter Source: SGD
  • protein phosphorylation Source: SGD
  • transcription, DNA-templated Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-34290-MONOMER
BRENDAi2.7.11.22 984

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase BUR1 (EC:2.7.11.22, EC:2.7.11.23)
Alternative name(s):
Bypass UAS requirement protein 1
Suppressor of GPA1-Vall50 mutation protein 1
Gene namesi
Name:SGV1
Synonyms:BUR1
Ordered Locus Names:YPR161C
ORF Names:P9584.8
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XVI

Organism-specific databases

EuPathDBiFungiDB:YPR161C
SGDiS000006365 SGV1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107E → Q: Loss of kinase activity in vitro. 1 Publication1
Mutagenesisi213D → N: Loss of kinase activity in vitro. 1 Publication1
Mutagenesisi240T → A, D or E: No phosphorylation of SGV1. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856871 – 657Serine/threonine-protein kinase BUR1Add BLAST657

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei240Phosphothreonine; by CAK1 Publication1
Modified residuei400PhosphoserineCombined sources1
Modified residuei405PhosphothreonineCombined sources1
Modified residuei417PhosphoserineCombined sources1
Modified residuei634PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23293
PaxDbiP23293
PRIDEiP23293

PTM databases

iPTMnetiP23293

Interactioni

Subunit structurei

Belongs to the BUR kinase complex composed of SGV1/BUR1 and BUR2. Interacts with BUR2 and RBP1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BUR2Q059496EBI-17078,EBI-30948

Protein-protein interaction databases

BioGridi36334, 1112 interactors
ComplexPortaliCPX-1685 BUR1-BUR2 kinase complex
DIPiDIP-5916N
IntActiP23293, 15 interactors
MINTiP23293
STRINGi4932.YPR161C

Structurei

3D structure databases

ProteinModelPortaliP23293
SMRiP23293
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 366Protein kinasePROSITE-ProRule annotationAdd BLAST307

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00910000144920
HOGENOMiHOG000233024
InParanoidiP23293
KOiK15562
OMAiMYDYEFE
OrthoDBiEOG092C2FL8

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P23293-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDNGSPAVL PKTEFNKYKI GKVKSTPAIQ RDAKTNLTYI KLRKRSSEKV
60 70 80 90 100
YGCTVFQNHY REDEKLGQGT FGEVYKGIHL ETQRQVAMKK IIVSVEKDLF
110 120 130 140 150
PITAQREITI LKRLNHKNII KLIEMVYDHS PDITNAASSN LHKSFYMILP
160 170 180 190 200
YMVADLSGVL HNPRINLEMC DIKNMMLQIL EGLNYIHCAK FMHRDIKTAN
210 220 230 240 250
ILIDHNGVLK LADFGLARLY YGCPPNLKYP GGAGSGAKYT SVVVTRWYRA
260 270 280 290 300
PELVLGDKQY TTAVDIWGVG CVFAEFFEKK PILQGKTDID QGHVIFKLLG
310 320 330 340 350
TPTEEDWAVA RYLPGAELTT TNYKPTLRER FGKYLSETGL DFLGQLLALD
360 370 380 390 400
PYKRLTAMSA KHHPWFKEDP LPSEKITLPT EESHEADIKR YKEEMHQSLS
410 420 430 440 450
QRVPTAPRGH IVEKGESPVV KNLGAIPRGP KKDDASFLPP SKNVLAKPPP
460 470 480 490 500
SKIRELHQNP RPYHVNSGYA KTAIPPPAAP AGVNRYGPNN SSRNNRFSGN
510 520 530 540 550
STAPNNSRNP VNRFHPETNV SSKYNKVPLP LGPQSRYQGN SNESRYKNSP
560 570 580 590 600
NDSRYHNPRY VNKPETNFNR QPQKYSRQES NAPINKNYNP SNGSRNMAGD
610 620 630 640 650
HHQGSRPSHP QFPISPSQGQ HQLTSKPIEK KNGSFKDERA KPDESKEFQN

SDIADLY
Length:657
Mass (Da):74,239
Last modified:November 1, 1991 - v1
Checksum:iCC9034A4B10A510E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D90317 Genomic DNA Translation: BAA14347.1
L33835 Genomic DNA Translation: AAB59314.1
U28371 Genomic DNA Translation: AAB68058.1
BK006949 Genomic DNA Translation: DAA11578.1
PIRiA39526
RefSeqiNP_015487.1, NM_001184258.1

Genome annotation databases

EnsemblFungiiYPR161C; YPR161C; YPR161C
GeneIDi856290
KEGGisce:YPR161C

Similar proteinsi

Entry informationi

Entry nameiBUR1_YEAST
AccessioniPrimary (citable) accession number: P23293
Secondary accession number(s): D6W4G2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 20, 2018
This is version 186 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health