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Protein

Casein kinase I homolog 2

Gene

YCK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates.1 Publication

Miscellaneous

Present with 6160 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei105ATPPROSITE-ProRule annotation1
Active sitei195Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi82 – 90ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • cell morphogenesis Source: SGD
  • endocytosis Source: SGD
  • glucose mediated signaling pathway Source: SGD
  • peptidyl-serine phosphorylation Source: GO_Central
  • protein phosphorylation Source: SGD
  • regulation of cell shape Source: GO_Central
  • response to glucose Source: SGD

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YNL154C-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Casein kinase I homolog 2 (EC:2.7.11.1)
Gene namesi
Name:YCK2
Synonyms:CKI1
Ordered Locus Names:YNL154C
ORF Names:N1755
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNL154C.
SGDiS000005098. YCK2.

Subcellular locationi

  • Cell membrane 1 Publication; Lipid-anchor 1 Publication

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cytoplasm Source: GO_Central
  • mating projection Source: SGD
  • nucleus Source: GO_Central
  • plasma membrane Source: SGD

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi545C → S: Impaired palmitoylation and plasma membrane localization. 1 Publication1
Mutagenesisi546C → S: Impaired palmitoylation and plasma membrane localization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001928572 – 546Casein kinase I homolog 2Add BLAST545

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei455PhosphoserineCombined sources1
Cross-linki465Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Lipidationi545S-palmitoyl cysteine2 Publications1
Lipidationi546S-palmitoyl cysteine2 Publications1

Post-translational modificationi

Palmitoylated by AKR1, which is required for proper plasma membrane localization of YCK2.2 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP23292.
PRIDEiP23292.

PTM databases

iPTMnetiP23292.
SwissPalmiP23292.

Interactioni

Protein-protein interaction databases

BioGridi35675. 460 interactors.
DIPiDIP-720N.
IntActiP23292. 9 interactors.
MINTiMINT-389928.

Structurei

3D structure databases

ProteinModelPortaliP23292.
SMRiP23292.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini76 – 360Protein kinasePROSITE-ProRule annotationAdd BLAST285

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00850000132311.
HOGENOMiHOG000182055.
InParanoidiP23292.
KOiK02218.
OMAiNPPNERE.
OrthoDBiEOG092C30PR.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23292-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQVQSPLTA TNSGLAVNNN TMNSQMPNRS NVRLVNGTLP PSLHVSSNLN
60 70 80 90 100
HNTGNSSASY SGSQSRDDST IVGLHYKIGK KIGEGSFGVL FEGTNMINGL
110 120 130 140 150
PVAIKFEPRK TEAPQLKDEY RTYKILAGTP GIPQEYYFGQ EGLHNILVID
160 170 180 190 200
LLGPSLEDLF DWCGRRFSVK TVVQVAVQMI TLIEDLHAHD LIYRDIKPDN
210 220 230 240 250
FLIGRPGQPD ANKVHLIDFG MAKQYRDPKT KQHIPYREKK SLSGTARYMS
260 270 280 290 300
INTHLGREQS RRDDMEAMGH VFFYFLRGQL PWQGLKAPNN KQKYEKIGEK
310 320 330 340 350
KRLTNVYDLA QGLPIQFGRY LEIVRNLSFE ETPDYEGYRM LLLSVLDDLG
360 370 380 390 400
ETADGQYDWM KLNGGRGWDL SINKKPNLHG YGHPNPPNEK SKRHRSKNHQ
410 420 430 440 450
YSSPDHHHHY NQQQQQQQAQ AQAQAQAQAK VQQQQLQQAQ AQQQANRYQL
460 470 480 490 500
QPDDSHYDEE REASKLDPTS YEAYQQQTQQ KYAQQQQKQM QQKSKQFANT
510 520 530 540
GANGQTNKYP YNAQPTANDE QNAKNAAQDR NSNKSSKGFF SKLGCC
Length:546
Mass (Da):62,079
Last modified:November 1, 1991 - v1
Checksum:iA88D99BC265BF4FA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74453 Genomic DNA. Translation: AAA35230.1.
X60326 mRNA. Translation: CAA42896.1.
X92517 Genomic DNA. Translation: CAA63285.1.
Z71430 Genomic DNA. Translation: CAA96041.1.
BK006947 Genomic DNA. Translation: DAA10395.1.
PIRiS29522.
RefSeqiNP_014245.1. NM_001182992.1.

Genome annotation databases

EnsemblFungiiYNL154C; YNL154C; YNL154C.
GeneIDi855568.
KEGGisce:YNL154C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74453 Genomic DNA. Translation: AAA35230.1.
X60326 mRNA. Translation: CAA42896.1.
X92517 Genomic DNA. Translation: CAA63285.1.
Z71430 Genomic DNA. Translation: CAA96041.1.
BK006947 Genomic DNA. Translation: DAA10395.1.
PIRiS29522.
RefSeqiNP_014245.1. NM_001182992.1.

3D structure databases

ProteinModelPortaliP23292.
SMRiP23292.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35675. 460 interactors.
DIPiDIP-720N.
IntActiP23292. 9 interactors.
MINTiMINT-389928.

PTM databases

iPTMnetiP23292.
SwissPalmiP23292.

Proteomic databases

MaxQBiP23292.
PRIDEiP23292.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNL154C; YNL154C; YNL154C.
GeneIDi855568.
KEGGisce:YNL154C.

Organism-specific databases

EuPathDBiFungiDB:YNL154C.
SGDiS000005098. YCK2.

Phylogenomic databases

GeneTreeiENSGT00850000132311.
HOGENOMiHOG000182055.
InParanoidiP23292.
KOiK02218.
OMAiNPPNERE.
OrthoDBiEOG092C30PR.

Enzyme and pathway databases

BioCyciYEAST:YNL154C-MONOMER.
BRENDAi2.7.11.1. 984.

Miscellaneous databases

PROiPR:P23292.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKC12_YEAST
AccessioniPrimary (citable) accession number: P23292
Secondary accession number(s): D6W129
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 12, 2017
This is version 165 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.