Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase 2B catalytic subunit A1

Gene

CNA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Miscellaneous

Present with 7040 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe3+ ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi119IronBy similarity1
Metal bindingi121IronBy similarity1
Metal bindingi147IronBy similarity1
Metal bindingi147ZincBy similarity1
Metal bindingi179ZincBy similarity1
Active sitei180Proton donorBy similarity1
Metal bindingi228ZincBy similarity1
Metal bindingi317ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
  • cellular ion homeostasis Source: SGD
  • fungal-type cell wall organization Source: SGD

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase, Protein phosphatase
LigandIron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32491-MONOMER
ReactomeiR-SCE-2871809 FCERI mediated Ca+2 mobilization
R-SCE-4086398 Ca2+ pathway
R-SCE-5607763 CLEC7A (Dectin-1) induces NFAT activation

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit A1 (EC:3.1.3.16)
Alternative name(s):
Calcineurin A1
Calmodulin-binding protein 1
Gene namesi
Name:CNA1
Synonyms:CMP1
Ordered Locus Names:YLR433C
ORF Names:L9753.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR433C
SGDiS000004425 CNA1

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000588362 – 553Serine/threonine-protein phosphatase 2B catalytic subunit A1Add BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP23287
PaxDbiP23287
PRIDEiP23287

PTM databases

iPTMnetiP23287

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
CMD1P067874EBI-12771,EBI-3976

GO - Molecular functioni

Protein-protein interaction databases

BioGridi31692, 119 interactors
ComplexPortaliCPX-588 Calcineurin complex variant 1
DIPiDIP-662N
IntActiP23287, 26 interactors
MINTiP23287
STRINGi4932.YLR433C

Structurei

Secondary structure

1553
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi455 – 458Combined sources4
Helixi460 – 474Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LHINMR-A453-476[»]
ProteinModelPortaliP23287
SMRiP23287
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00530000063087
HOGENOMiHOG000172699
InParanoidiP23287
KOiK04348
OMAiLWSLKIW
OrthoDBiEOG092C1CSB

Family and domain databases

Gene3Di3.60.21.10, 1 hit
InterProiView protein in InterPro
IPR004843 Calcineurin-like_PHP_ApaH
IPR029052 Metallo-depent_PP-like
IPR006186 Ser/Thr-sp_prot-phosphatase
PfamiView protein in Pfam
PF00149 Metallophos, 1 hit
PRINTSiPR00114 STPHPHTASE
SMARTiView protein in SMART
SM00156 PP2Ac, 1 hit
PROSITEiView protein in PROSITE
PS00125 SER_THR_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKDLNSSRI KIIKPNDSYI KVDRKKDLTK YELENGKVIS TKDRPIASVP
60 70 80 90 100
AITGKIPSDE EVFDSKTGLP NHSFLREHFF HEGRLSKEQA IKILNMSTVA
110 120 130 140 150
LSKEPNLLKL KAPITICGDI HGQYYDLLKL FEVGGDPAEI DYLFLGDYVD
160 170 180 190 200
RGAFSFECLI YLYSLKLNNL GRFWMLRGNH ECKHLTSYFT FKNEMLHKYD
210 220 230 240 250
MEVYDACCRS FNVLPLAALM NGQYFCVHGG ISPELKSVED VNKINRFREI
260 270 280 290 300
PSRGLMCDLL WADPVENYDD ARDGSEFDQS EDEFVPNSLR GCSFAFTFKA
310 320 330 340 350
SCKFLKANGL LSIIRAHEAQ DAGYRMYKNN KVTGFPSLIT MFSAPNYLDT
360 370 380 390 400
YHNKAAVLKY EENVMNIRQF HMSPHPYWLP DFMDVFTWSL PFVGEKVTSM
410 420 430 440 450
LVSILNICSE QELDPESEPK AAEETVKARA NATKETGTPS DEKASSAILE
460 470 480 490 500
DETRRKALRN KILAIAKVSR MFSVLREESE KVEYLKTMNA GVLPRGALAR
510 520 530 540 550
GTEGLNETLS TFEKARKEDL INEKLPPSLS EVEQEKIKYY EKILKGAEKK

PQL
Length:553
Mass (Da):63,002
Last modified:October 1, 1993 - v2
Checksum:iD5D6F91CC0BD277B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti45 – 46PI → SY in strain: S288c / GRF88. 2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64839 Genomic DNA Translation: AAA34465.1
X66490 Genomic DNA Translation: CAA47117.1
X54963 Genomic DNA Translation: CAA38711.1
U21094 Genomic DNA Translation: AAB67518.1
AY693079 Genomic DNA Translation: AAT93098.1
BK006945 Genomic DNA Translation: DAA09734.1
PIRiS16809
RefSeqiNP_013537.1, NM_001182321.1

Genome annotation databases

EnsemblFungiiYLR433C; YLR433C; YLR433C
GeneIDi851153
KEGGisce:YLR433C

Similar proteinsi

Entry informationi

Entry nameiPP2B1_YEAST
AccessioniPrimary (citable) accession number: P23287
Secondary accession number(s): D6VZ68
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1993
Last modified: June 20, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health