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P23287

- PP2B1_YEAST

UniProt

P23287 - PP2B1_YEAST

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Protein

Serine/threonine-protein phosphatase 2B catalytic subunit A1

Gene

CNA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Protein has several cofactor binding sites:
  • Fe3+By similarityNote: Binds 1 Fe(3+) ion per subunit.By similarity
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191IronBy similarity
Metal bindingi121 – 1211IronBy similarity
Metal bindingi147 – 1471IronBy similarity
Metal bindingi147 – 1471ZincBy similarity
Metal bindingi179 – 1791ZincBy similarity
Active sitei180 – 1801Proton donorBy similarity
Metal bindingi228 – 2281ZincBy similarity
Metal bindingi317 – 3171ZincBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. phosphoprotein phosphatase activity Source: UniProtKB-KW

GO - Biological processi

  1. adaptation of signaling pathway by response to pheromone involved in conjugation with cellular fusion Source: SGD
  2. cellular ion homeostasis Source: SGD
  3. dephosphorylation Source: GOC
  4. fungal-type cell wall organization Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Calmodulin-binding, Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32491-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 2B catalytic subunit A1 (EC:3.1.3.16)
Alternative name(s):
Calcineurin A1
Calmodulin-binding protein 1
Gene namesi
Name:CNA1
Synonyms:CMP1
Ordered Locus Names:YLR433C
ORF Names:L9753.6
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

CYGDiYLR433c.
SGDiS000004425. CNA1.

Subcellular locationi

GO - Cellular componenti

  1. calcineurin complex Source: SGD
  2. cytoplasm Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 553552Serine/threonine-protein phosphatase 2B catalytic subunit A1PRO_0000058836Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP23287.
PaxDbiP23287.
PeptideAtlasiP23287.

Expressioni

Gene expression databases

GenevestigatoriP23287.

Interactioni

Subunit structurei

Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity.

Binary interactionsi

WithEntry#Exp.IntActNotes
CMD1P067873EBI-12771,EBI-3976

Protein-protein interaction databases

BioGridi31692. 67 interactions.
DIPiDIP-662N.
IntActiP23287. 23 interactions.
MINTiMINT-397444.
STRINGi4932.YLR433C.

Structurei

Secondary structure

1
553
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi455 – 4584Combined sources
Helixi460 – 47415Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LHINMR-A453-476[»]
ProteinModelPortaliP23287.
SMRiP23287. Positions 13-408.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2B subfamily.Curated

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00530000063087.
HOGENOMiHOG000172699.
InParanoidiP23287.
KOiK04348.
OMAiHTIQTFE.
OrthoDBiEOG77M8X9.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 2 hits.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23287-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSKDLNSSRI KIIKPNDSYI KVDRKKDLTK YELENGKVIS TKDRPIASVP
60 70 80 90 100
AITGKIPSDE EVFDSKTGLP NHSFLREHFF HEGRLSKEQA IKILNMSTVA
110 120 130 140 150
LSKEPNLLKL KAPITICGDI HGQYYDLLKL FEVGGDPAEI DYLFLGDYVD
160 170 180 190 200
RGAFSFECLI YLYSLKLNNL GRFWMLRGNH ECKHLTSYFT FKNEMLHKYD
210 220 230 240 250
MEVYDACCRS FNVLPLAALM NGQYFCVHGG ISPELKSVED VNKINRFREI
260 270 280 290 300
PSRGLMCDLL WADPVENYDD ARDGSEFDQS EDEFVPNSLR GCSFAFTFKA
310 320 330 340 350
SCKFLKANGL LSIIRAHEAQ DAGYRMYKNN KVTGFPSLIT MFSAPNYLDT
360 370 380 390 400
YHNKAAVLKY EENVMNIRQF HMSPHPYWLP DFMDVFTWSL PFVGEKVTSM
410 420 430 440 450
LVSILNICSE QELDPESEPK AAEETVKARA NATKETGTPS DEKASSAILE
460 470 480 490 500
DETRRKALRN KILAIAKVSR MFSVLREESE KVEYLKTMNA GVLPRGALAR
510 520 530 540 550
GTEGLNETLS TFEKARKEDL INEKLPPSLS EVEQEKIKYY EKILKGAEKK

PQL
Length:553
Mass (Da):63,002
Last modified:October 1, 1993 - v2
Checksum:iD5D6F91CC0BD277B
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti45 – 462PI → SY in strain: S288c / GRF88.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64839 Genomic DNA. Translation: AAA34465.1.
X66490 Genomic DNA. Translation: CAA47117.1.
X54963 Genomic DNA. Translation: CAA38711.1.
U21094 Genomic DNA. Translation: AAB67518.1.
AY693079 Genomic DNA. Translation: AAT93098.1.
BK006945 Genomic DNA. Translation: DAA09734.1.
PIRiS16809.
RefSeqiNP_013537.1. NM_001182321.1.

Genome annotation databases

EnsemblFungiiYLR433C; YLR433C; YLR433C.
GeneIDi851153.
KEGGisce:YLR433C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64839 Genomic DNA. Translation: AAA34465.1 .
X66490 Genomic DNA. Translation: CAA47117.1 .
X54963 Genomic DNA. Translation: CAA38711.1 .
U21094 Genomic DNA. Translation: AAB67518.1 .
AY693079 Genomic DNA. Translation: AAT93098.1 .
BK006945 Genomic DNA. Translation: DAA09734.1 .
PIRi S16809.
RefSeqi NP_013537.1. NM_001182321.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LHI NMR - A 453-476 [» ]
ProteinModelPortali P23287.
SMRi P23287. Positions 13-408.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31692. 67 interactions.
DIPi DIP-662N.
IntActi P23287. 23 interactions.
MINTi MINT-397444.
STRINGi 4932.YLR433C.

Proteomic databases

MaxQBi P23287.
PaxDbi P23287.
PeptideAtlasi P23287.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR433C ; YLR433C ; YLR433C .
GeneIDi 851153.
KEGGi sce:YLR433C.

Organism-specific databases

CYGDi YLR433c.
SGDi S000004425. CNA1.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00530000063087.
HOGENOMi HOG000172699.
InParanoidi P23287.
KOi K04348.
OMAi HTIQTFE.
OrthoDBi EOG77M8X9.

Enzyme and pathway databases

BioCyci YEAST:G3O-32491-MONOMER.

Miscellaneous databases

NextBioi 967933.

Gene expression databases

Genevestigatori P23287.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 2 hits.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast has homologs (CNA1 and CNA2 gene products) of mammalian calcineurin, a calmodulin-regulated phosphoprotein phosphatase."
    Cyert M.S., Kunisawa R., Kaim D., Thorner J.
    Proc. Natl. Acad. Sci. U.S.A. 88:7376-7380(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: S288c / GRF88.
  2. "Identification and molecular characterization of the calmodulin-binding subunit gene (CMP1) of protein phosphatase 2B from Saccharomyces cerevisiae. An alpha-factor inducible gene."
    Ye R.R., Bretscher A.
    Eur. J. Biochem. 204:713-723(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 20B-12.
  3. "The Saccharomyces cerevisiae genes (CMP1 and CMP2) encoding calmodulin-binding proteins homologous to the catalytic subunit of mammalian protein phosphatase 2B."
    Liu Y., Ishii S., Tokai M., Tsutsumi H., Ohki O., Akada R., Tanaka K., Tsuchiya E., Fukui S., Miyakawa T.
    Mol. Gen. Genet. 227:52-59(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPP2B1_YEAST
AccessioniPrimary (citable) accession number: P23287
Secondary accession number(s): D6VZ68
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1993
Last modified: November 26, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7040 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3