Reviewed, UniProtKB/Swiss-Prot P23285 (CYPB_YEAST)

Names and origin

Protein names

Recommended name:
    Peptidyl-prolyl cis-trans isomerase B
      Short name=PPIase
      Short name=Rotamase
    EC=5.2.1.8
Alternative name(s):
    Cyclophilin B
    Cyclophilin-related protein

Gene names

Name:	CPR2
Synonyms:	CRG, CYP2, SCC2
Ordered Locus Names:	YHR057C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Catalytic activity	Peptidylproline (omega=180) = peptidylproline (omega=0).
Enzyme regulation	Cyclosporin A (CsA) inhibits CYPB.
Subcellular location	Secreted.
Miscellaneous	Present with 339 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the cyclophilin-type PPIase family. PPIase B subfamily. Contains 1 PPIase cyclophilin-type domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Cyclosporin
Molecular function	Isomerase Rotamase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	peptide binding Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-prolyl cis-trans isomerase activity Ref.4 Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	P38800	1	EBI-5448,EBI-24597
GET3	Q12154	1	EBI-5448,EBI-2989

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 205	185	Peptidyl-prolyl cis-trans isomerase B		PRO_0000025486
Regions
	Domain	39 – 198	160	PPIase cyclophilin-type

Sequences

	Sequence		Length	Mass (Da)
	P23285-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 616EAEB434837A6D	FASTA	205	22,769
	10 20 30 40 50 60 MKFSGLWCWL LLFLSVNVIA SDVGELIDQD DEVITQKVFF DIEHGEEKVG RIVIGLYGKV 70 80 90 100 110 120 CPKTAKNFYK LSTTTNSKKG FIGSTFHRVI PNFMVQGGDF TDGTGVGGKS IYGDTFPDEN 130 140 150 160 170 180 FTLKHDRKGR LSMANRGKDT NGSQFFITTT EEASWLDGKH VVFGQVVDGM DVVNYIQHVS 190 200 RDANDKPLEA VKIAKCGEWT PELSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second cyclophilin-related gene in Saccharomyces cerevisiae." Koser P., Sylvester D., Livi G.P., Bergsma D.J. Nucleic Acids Res. 18:1643-1643(1990) [PubMed: 2183199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"The CYP2 gene of Saccharomyces cerevisiae encodes a cyclosporin A-sensitive peptidyl-prolyl cis-trans isomerase with an N-terminal signal sequence." Koser P., Bergsma D.J., Cafferkey R., Eng W.-K., McLaughlin M.M., Ferrara A., Silverman C., Kasyan K., Bossard M.J., Johnson R.K., Porter T.G., Levy M.A., Livi G.P. Gene 108:73-80(1991) [PubMed: 1761234] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X51497 Genomic DNA. Translation: CAA35865.1. Sequence problems. U00061 Genomic DNA. Translation: AAB68386.1. AY558279 Genomic DNA. Translation: AAS56605.1.
PIR	S12324.
RefSeq	NP_011924.1.
3D structure databases
HSSP	HSSP built from PDB template 1CYN based on UniProtKB P23284.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1280N.
IntAct	P23285. 6 interactions.
Genome annotation databases
Ensembl	YHR057C. Saccharomyces cerevisiae. [Contig view]
GeneID	856454.
GenomeReviews	Gene locus YHR057C in contig U00093_GR.
KEGG	sce:YHR057C.
NMPDR	fig|4932.3.peg.3077.
Organism-specific databases
CYGD	YHR057c.
SGD	S000001099. CPR2.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P23285.
OMA	P23285. MANAGED.
Enzyme and pathway databases
BRENDA	5.2.1.8. 250.
Gene expression databases
GermOnline	YHR057C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002130. PPIase_cyclophilin. [Graphical view]
Gene3D	G3DSA:2.40.100.10. PPIase_cyclophilin. 1 hit.
Pfam	PF00160. Pro_isomerase. 1 hit. [Graphical view]
PRINTS	PR00153. CSAPPISMRASE.
PROSITE	PS00170. CSA_PPIASE_1. 1 hit. PS50072. CSA_PPIASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	982087.

Entry information

Entry name

CYPB_YEAST

Accession

Primary (citable) accession number: P23285

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)