Reviewed, UniProtKB/Swiss-Prot P23281 (AROA2_TOBAC)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase 2
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase 2
      Short name=EPSP synthase 2

Gene names

Name:

EPSPS-2

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	338 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.
Subcellular location	Plastid › chloroplast.
Miscellaneous	This enzyme is the target of the potent, broad-spectrum herbicide, glyphosate [n-(phosphonomethyl)glycine]. Overproduction of EPSP leads to glyphosate tolerance.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Molecular function	Transferase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 338	›338	3-phosphoshikimate 1-carboxyvinyltransferase 2		PRO_0000088341
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P23281-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 569E90218AD68B89	FASTA	338	36,319
	10 20 30 40 50 60 LTAAVAVAGG NSRYVLDGVP RMRERPIGDL VDGLKQLGAE VDCFLGTKCP PVRIVSKGGL 70 80 90 100 110 120 PGGKVKLSGS ISSQYLTALL MAAPLALGDV EIEIIDKLIS VLYVEMTLKL MERFGISVEH 130 140 150 160 170 180 SSSWDRFVVR GGQKYKSPGK AYVEGDASSA SYFLAGAAVT GGTVTVEGCG TSSLQGDVKF 190 200 210 220 230 240 AEVLEQMGAE VTWTENSVTV KGPPRNSSAM KHLRAIDVNM NKMPDVAMTL AVVALFADGP 250 260 270 280 290 300 TAIRDVASWR VKETERMIAI CTELRKLGAT VEEGPDYCII TPPEKLNVTE IDTYDDHRMA 310 320 330 MAFSLAACAD VPVTINDPGC TRKTFPNYFD VLQQYSKH

References

[1]	"Expression and stability of amplified genes encoding 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate-tolerant tobacco cells." Wang Y., Jones J., Weller S., Goldsbrough P.B. Plant Mol. Biol. 17:1127-1138(1991) [PubMed: 1932690] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M61905 mRNA. Translation: AAA34072.1.
PIR	S18354.
3D structure databases
HSSP	HSSP built from PDB template 1G6S based on UniProtKB P07638.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.19. 298.
Family and domain databases
InterPro	IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. Partial match. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA2_TOBAC

Accession

Primary (citable) accession number: P23281

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	September 22, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)