Reviewed, UniProtKB/Swiss-Prot P23257 (TBG1_DROME)

Names and origin

Protein names

Recommended name:
    Tubulin gamma-1 chain
Alternative name(s):
    Gamma-1-tubulin

Gene names

Name:	gammaTub23C
Synonyms:	Tub23C, TubG, TubG1, TubG23C
ORF Names:	CG3157

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome, suggesting that it is involved in the minus-end nucleation of microtubule assembly.
Sequence similarities	Belongs to the tubulin family.

Ontologies

Keywords
Cellular component	Microtubule
Ligand	GTP-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	microtubule-based process Inferred from electronic annotation. Source: InterPro protein polymerization Inferred from electronic annotation. Source: InterPro
Cellular component	microtubule Inferred from electronic annotation. Source: UniProtKB-KW protein complex Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q8IMB9	1	EBI-178952,EBI-115118

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Tubulin gamma-1 chain		PRO_0000048454
Regions
	Nucleotide binding	142 – 148	7	GTP Potential
Experimental info
	Sequence conflict	402 – 403	2	KL → NV in AAA28597. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23257-1 [UniParc]. Last modified March 15, 2004. Version 2. Checksum: AD35304CA1490262	FASTA	475	53,339
	10 20 30 40 50 60 MPSEIITLQL GQCGNQIGFE FWKRLCLEHG ISPSGVLEDF ANDGLDRKDV FFYQADDDHY 70 80 90 100 110 120 IPRAVLLDLE PRVINTIMGS VYSKLYNPEN VYLSKHGGGA GNNWASGYSQ GEKLQEEVFD 130 140 150 160 170 180 IIDREADGSD SLEGFILCHS IAGGTGSGMG SFIMERLADR YPKKLIQTFS VFPNQDEISD 190 200 210 220 230 240 VVVQPYNSML TLKRLTTAAD SVVVLDNTAL NRIACDRLHI QNPSFSQINN LVSTIMSVST 250 260 270 280 290 300 TTLRYPSYMN NNLIGLTAPL IPTPQLHFLM TGYTPLTSDS DIHTQQLVNV RKTTVLDVMR 310 320 330 340 350 360 RLLQPKNMMV STGPDKSNHH CYISILNIIQ GEVDPTQVHK SLQRIRDRKM AQFIPWGPTS 370 380 390 400 410 420 IQVALSRSSP YVQSNHRVSG LMLANHTSIC SLFERALNQY DKLRKRGAFL DQFRREDIFK 430 440 450 460 470 DDLNELDESR ETVDCLVQEY EAATREDYMQ FSVKRGNGPV DSKSEDSRSV TSAGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome." Zheng Y., Jung M.K., Oakley B.R. Cell 65:817-823(1991) [PubMed: 1904010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Genomic organization of the Drosophlia 23C genetic interval: identification of 3 genes in the 10Kb region surrounding the RRP1 gene." Tsoi S.C.M., Huang S.M., Sander M. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-333. Strain: Canton-S.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M61765 mRNA. Translation: AAA28597.1. AE014134 Genomic DNA. Translation: AAF51174.1. AY069633 mRNA. Translation: AAL39778.1. AF073994 Genomic DNA. Translation: AAC27620.1.
PIR	UBFFG. B39527.
RefSeq	NP_476804.1.
UniGene	Dm.2033
3D structure databases
HSSP	HSSP built from PDB template 1TUB based on UniProtKB P02554.
ModBase	Search...
Protein-protein interaction databases
IntAct	P23257. 4 interactions.
Genome annotation databases
Ensembl	FBgn0004176. Drosophila melanogaster. [Contig view]
GeneID	33501.
KEGG	dme:Dmel_CG3157.
NMPDR	fig|7227.3.peg.505.
Organism-specific databases
FlyBase	FBgn0004176. gammaTub23C.
Phylogenomic databases
HOGENOM	P23257.
OMA	P23257. SHCYIAI.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-007110-MON.
Gene expression databases
ArrayExpress	P23257.
GermOnline	CG3157. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002454. Gamma_tubulin. IPR000217. Tubulin. IPR018316. Tubulin/FtsZ_2-layer-sand-dom. IPR017975. Tubulin_CS. IPR003008. Tubulin_FtsZ_GTPase. [Graphical view]
Gene3D	G3DSA:3.30.1330.20. Tubulin/FtsZ_2-layer-sand-dom. 1 hit. G3DSA:3.40.50.1440. Tubulin_FtsZ. 1 hit.
PANTHER	PTHR11588. Tubulin. 1 hit.
Pfam	PF00091. Tubulin. 1 hit. PF03953. Tubulin_C. 1 hit. [Graphical view]
PRINTS	PR01164. GAMMATUBULIN. PR01161. TUBULIN.
PROSITE	PS00227. TUBULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	783917.

Entry information

Entry name

TBG1_DROME

Accession

Primary (citable) accession number: P23257
Secondary accession number(s): Q9VQJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	March 15, 2004
Last modified:	June 16, 2009
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project