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Protein

Protein MalY

Gene

malY

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a beta-cystathionase and as a repressor of the maltose regulon.

Catalytic activityi

L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.1 Publication

Cofactori

pyridoxal 5'-phosphate2 Publications

GO - Molecular functioni

  • cystathionine beta-lyase activity Source: EcoCyc
  • L-cysteine desulfhydrase activity Source: EcoCyc
  • pyridoxal phosphate binding Source: EcoCyc

GO - Biological processi

Keywordsi

Molecular functionLyase, Repressor
Biological processAmino-acid biosynthesis, Methionine biosynthesis, Transcription, Transcription regulation
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciEcoCyc:EG10564-MONOMER
MetaCyc:EG10564-MONOMER
SABIO-RKiP23256

Protein family/group databases

MoonProtiP23256

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MalY
Including the following 2 domains:
Cystathionine beta-lyase MalY (EC:4.4.1.81 Publication)
Short name:
CBL
Alternative name(s):
Beta-cystathionase
Cysteine lyase
Maltose regulon modulator
Gene namesi
Name:malY
Ordered Locus Names:b1622, JW1614
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10564 malY

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi233K → I: Loss of enzymatic activity; but no loss of repression function. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001638471 – 390Protein MalYAdd BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei233N6-(pyridoxal phosphate)lysine2 Publications1

Proteomic databases

PaxDbiP23256
PRIDEiP23256

Interactioni

Subunit structurei

Homodimer. Interacts with MalT.1 Publication

Protein-protein interaction databases

BioGridi4261731, 9 interactors
DIPiDIP-10151N
IntActiP23256, 10 interactors
STRINGi316385.ECDH10B_1755

Structurei

Secondary structure

1390
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi35 – 37Combined sources3
Helixi44 – 54Combined sources11
Helixi67 – 80Combined sources14
Helixi87 – 89Combined sources3
Beta strandi90 – 94Combined sources5
Helixi96 – 106Combined sources11
Beta strandi113 – 119Combined sources7
Helixi122 – 130Combined sources9
Beta strandi134 – 139Combined sources6
Beta strandi144 – 148Combined sources5
Helixi151 – 158Combined sources8
Beta strandi163 – 171Combined sources9
Turni173 – 175Combined sources3
Helixi183 – 193Combined sources11
Beta strandi197 – 201Combined sources5
Turni203 – 206Combined sources4
Beta strandi210 – 212Combined sources3
Helixi217 – 219Combined sources3
Beta strandi223 – 229Combined sources7
Helixi232 – 235Combined sources4
Helixi238 – 240Combined sources3
Beta strandi243 – 247Combined sources5
Helixi250 – 261Combined sources12
Helixi271 – 283Combined sources13
Helixi285 – 309Combined sources25
Beta strandi322 – 327Combined sources6
Helixi329 – 331Combined sources3
Helixi335 – 344Combined sources10
Helixi353 – 356Combined sources4
Helixi358 – 360Combined sources3
Beta strandi363 – 367Combined sources5
Helixi372 – 389Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D2FX-ray2.50A/B1-390[»]
ProteinModelPortaliP23256
SMRiP23256
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23256

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C87 Bacteria
COG1168 LUCA
HOGENOMiHOG000223048
InParanoidiP23256
KOiK14155
OMAiRINIGCP
PhylomeDBiP23256

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR027619 C_S_lyase_PatB
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
TIGRFAMsiTIGR04350 C_S_lyase_PatB, 1 hit

Sequencei

Sequence statusi: Complete.

P23256-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDFSKVVDR HGTWCTQWDY VADRFGTADL LPFTISDMDF ATAPCIIEAL
60 70 80 90 100
NQRLMHGVFG YSRWKNDEFL AAIAHWFSTQ HYTAIDSQTV VYGPSVIYMV
110 120 130 140 150
SELIRQWSET GEGVVIHTPA YDAFYKAIEG NQRTVMPVAL EKQADGWFCD
160 170 180 190 200
MGKLEAVLAK PECKIMLLCS PQNPTGKVWT CDELEIMADL CERHGVRVIS
210 220 230 240 250
DEIHMDMVWG EQPHIPWSNV ARGDWALLTS GSKSFNIPAL TGAYGIIENS
260 270 280 290 300
SSRDAYLSAL KGRDGLSSPS VLALTAHIAA YQQGAPWLDA LRIYLKDNLT
310 320 330 340 350
YIADKMNAAF PELNWQIPQS TYLAWLDLRP LNIDDNALQK ALIEQEKVAI
360 370 380 390
MPGYTYGEEG RGFVRLNAGC PRSKLEKGVA GLINAIRAVR
Length:390
Mass (Da):43,642
Last modified:November 1, 1991 - v1
Checksum:i7FAF15D76545DDB0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60722 mRNA Translation: AAA24099.1
U00096 Genomic DNA Translation: AAC74694.1
AP009048 Genomic DNA Translation: BAA15373.1
PIRiC42477
RefSeqiNP_416139.1, NC_000913.3
WP_000459379.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74694; AAC74694; b1622
BAA15373; BAA15373; BAA15373
GeneIDi945937
KEGGiecj:JW1614
eco:b1622
PATRICifig|1411691.4.peg.639

Similar proteinsi

Entry informationi

Entry nameiMALY_ECOLI
AccessioniPrimary (citable) accession number: P23256
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 25, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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