P23255 (TAF2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 2

Alternative name(s):
TAFII-150
TBP-associated factor 150 kDa
TBP-associated factor 2
TSM-1

Gene names

Name:	TAF2
Synonyms:	TAF150, TSM1
Ordered Locus Names:	YCR042C
ORF Names:	YCR42C, YCR724

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1407 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. Ref.6 Ref.7 Ref.8
Subunit structure	The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14. Ref.6
Subcellular location	Nucleus.
Miscellaneous	Present with 1200 molecules/cell in log phase SD medium. Ref.9
Sequence similarities	Belongs to the TAF2 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	RNA polymerase II transcriptional preinitiation complex assembly Inferred by curator Ref.6. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	transcription factor TFIID complex Inferred from direct assay Ref.6. Source: SGD
Molecular function	chromatin binding Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SPT15	P13393	2	EBI-18862,EBI-19129
TAF1	P46677	4	EBI-18862,EBI-18855
TAF5	P38129	10	EBI-18862,EBI-18868

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1407	1407	Transcription initiation factor TFIID subunit 2		PRO_0000118867
Regions
	Region	1285 – 1350	66	Highly charged
	Coiled coil	304 – 337	34	Potential
	Compositional bias	52 – 55	4	Poly-Ile
	Compositional bias	259 – 334	76	Asp/Glu-rich (acidic)
Amino acid modifications
	Modified residue	18	1	Phosphoserine Ref.14
	Modified residue	19	1	Phosphothreonine Ref.13 Ref.14
	Modified residue	158	1	Phosphothreonine Ref.14
	Modified residue	161	1	Phosphothreonine Ref.14
	Modified residue	163	1	Phosphoserine Ref.14
	Modified residue	166	1	Phosphothreonine Ref.14
	Modified residue	268	1	Phosphothreonine Ref.12
	Modified residue	318	1	Phosphoserine Ref.11 Ref.13 Ref.14
Experimental info
	Sequence conflict	323	1	E → G in AAA35179. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P23255 [UniParc]. Last modified May 1, 1992. Version 3. Checksum: 184C8ED735443F38	FASTA	1,407	161,471
	10 20 30 40 50 60 MMSFSKNATP RAIVSESSTL HEMKFRNFRV AHEKISLDID LATHCITGSA TIIIIPLIQN 70 80 90 100 110 120 LEYVTFDCKE MTIKDVLVEN RRCDQFIHDD PLQTNLNGLT SQNVLYSDNS IEQSHFLRSK 130 140 150 160 170 180 FASLNEYPET DSKSQLTIKI PSSIKISLED ANALSNYTPI TPSIKTTPGF QESVFTPITL 190 200 210 220 230 240 QIEYEIRNPK SGIKFDTVYA DKPWLWNVYT SNGEICSSAS YWVPCVDLLD EKSTWELEFS 250 260 270 280 290 300 VPRLVKNIGT SKLIGQNGEE SEKEKEDTPE HDEEEEGKPA RVIKDEDKDS NLKNDEEGKN 310 320 330 340 350 360 SKSKDAQDND EEEEEGESDE EEEEGEEERR NIEESNNPSL RDVIVCCSEY SNIKELPHPI 370 380 390 400 410 420 DLTKKKCIFQ IINPVAPHHI GWAIGAFNSW SLPLISPPSV DAEDEVEEDK LRENVVDNVN 430 440 450 460 470 480 DTMDDDIGSD IIPIQIFTLP TQETDELTVI NSTVVCQKII DFYSKEFGSY PFTCYSMVFL 490 500 510 520 530 540 PTAPSKHMDF AALGICNTRL LYPLEVIDKA FSTTNELAWA LANQWSCVNI TPLDMNDYWC 550 560 570 580 590 600 CLGIAGYMVF QVTKKLMGNN TYKYQLKRNS EAIVEQDFEK PPIGSTFTGS SRPISWSSKD 610 620 630 640 650 660 LSFIQLKAPM ILHILDRRMT KTERSFGMSR VLPKIFLQAM SGDLPNNSLT SSHFQHVCER 670 680 690 700 710 720 VNKSKLENFF NEWVYGSGVP ILRVTQRFNR KRMVIELGIR QVQDEELGHE KVVGEEGFFK 730 740 750 760 770 780 SALDHLEHPD LNRTECFTGS MTIRIHEHDG TPYEHIVEIK DTFTKIDIQY NTKYRRLRKR 790 800 810 820 830 840 GGGANDENGV ENNNEEKPIV VDVNCLGNVY MSPEECSRFS LTEFNRTSES NELLKQNEAF 850 860 870 880 890 900 EWIRIDSDLE WICQMHINQP DYMFSSQLRQ DGDIEAQLEA IRYYEDVVVN GGVKSLVYSS 910 920 930 940 950 960 ILFRTAIDER YFFGIRLAAC EALSKYVYDP DFTGGVKHLI QIFQILFCLE DSNIPKSNNF 970 980 990 1000 1010 1020 ENPKLYFLQC NIPKYLAKVK NENGKCPKLV KQFLLDILVY NENGENKYSD DAYVRSLIEN 1030 1040 1050 1060 1070 1080 VVKVALNEYK DKAYMEKVKT QLLRYENLVN WLSSYESLIK TTIMYAKYKL HKVGAYDFTE 1090 1100 1110 1120 1130 1140 LTGMIMHTLT LGINNGDISR ESFQNEFLMV LKIMLLEGGL KNKDALVLFT EILCFHEDSY 1150 1160 1170 1180 1190 1200 IRDKSVDVLS ECVNLVVMDG SLDTISDDIK SSVQSVHNEV KNIKSEDDIE LFLSGHYVDD 1210 1220 1230 1240 1250 1260 MKIKIEKIGR QNISGLIQIC RDMFKGYSPL KILLWDVLNL PVLSLYQRKQ IHDLVRVMYT 1270 1280 1290 1300 1310 1320 LINSFVVRLE TPRERRLVAK MNSNEEGKLD IVIKRESILK VHIKKEVTST VEAPKKANKI 1330 1340 1350 1360 1370 1380 KISLKGDKPV RKVEKQIVKP KVTSKQRKVK SHVNRMGSLP LRFVKIQQQP RVMVHLSSVP 1390 1400 YSQFVQITKV TSRSFMVKIR TKNDAKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The MAT locus revisited within a 9.8 kb fragment of chromosome III containing BUD5 and two new open reading frames." Jacquet M., Buhler J.-M., Iborra F., Francingues-Gaillard M.-C., Soustelle C. Yeast 7:881-888(1991) [PubMed: 1789011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The TSM1 gene of Saccharomyces cerevisiae overlaps the MAT locus." Ray B.L., White C.I., Haber J.E. Curr. Genet. 20:25-31(1991) [PubMed: 1840512] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RY570.
[3]	"The complete DNA sequence of yeast chromosome III." Oliver S.G., van der Aart Q.J.M., Agostoni-Carbone M.L., Aigle M., Alberghina L., Alexandraki D., Antoine G., Anwar R., Ballesta J.P.G., Benit P., Berben G., Bergantino E., Biteau N., Bolle P.-A., Bolotin-Fukuhara M., Brown A., Brown A.J.P., Buhler J.-M. , Carcano C., Carignani G., Cederberg H., Chanet R., Contreras R., Crouzet M., Daignan-Fornier B., Defoor E., Delgado M.D., Demolder J., Doira C., Dubois E., Dujon B., Duesterhoeft A., Erdmann D., Esteban M., Fabre F., Fairhead C., Faye G., Feldmann H., Fiers W., Francingues-Gaillard M.-C., Franco L., Frontali L., Fukuhara H., Fuller L.J., Galland P., Gent M.E., Gigot D., Gilliquet V., Glansdorff N., Goffeau A., Grenson M., Grisanti P., Grivell L.A., de Haan M., Haasemann M., Hatat D., Hoenicka J., Hegemann J.H., Herbert C.J., Hilger F., Hohmann S., Hollenberg C.P., Huse K., Iborra F., Indge K.J., Isono K., Jacq C., Jacquet M., James C.M., Jauniaux J.-C., Jia Y., Jimenez A., Kelly A., Kleinhans U., Kreisl P., Lanfranchi G., Lewis C., van der Linden C.G., Lucchini G., Lutzenkirchen K., Maat M.J., Mallet L., Mannhaupt G., Martegani E., Mathieu A., Maurer C.T.C., McConnell D., McKee R.A., Messenguy F., Mewes H.-W., Molemans F., Montague M.A., Muzi Falconi M., Navas L., Newlon C.S., Noone D., Pallier C., Panzeri L., Pearson B.M., Perea J., Philippsen P., Pierard A., Planta R.J., Plevani P., Poetsch B., Pohl F.M., Purnelle B., Ramezani Rad M., Rasmussen S.W., Raynal A., Remacha M.A., Richterich P., Roberts A.B., Rodriguez F., Sanz E., Schaaff-Gerstenschlaeger I., Scherens B., Schweitzer B., Shu Y., Skala J., Slonimski P.P., Sor F., Soustelle C., Spiegelberg R., Stateva L.I., Steensma H.Y., Steiner S., Thierry A., Thireos G., Tzermia M., Urrestarazu L.A., Valle G., Vetter I., van Vliet-Reedijk J.C., Voet M., Volckaert G., Vreken P., Wang H., Warmington J.R., von Wettstein D., Wicksteed B.L., Wilson C., Wurst H., Xu G., Yoshikawa A., Zimmermann F.K., Sgouros J.G. Nature 357:38-46(1992) [PubMed: 1574125] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Identification and characterization of a TFIID-like multiprotein complex from Saccharomyces cerevisiae." Poon D., Bai Y., Campbell A.M., Bjorklund S., Kim Y.-J., Zhou S., Kornberg R.D., Weil P.A. Proc. Natl. Acad. Sci. U.S.A. 92:8224-8228(1995) [PubMed: 7667272] [Abstract] Cited for: PROTEIN SEQUENCE OF 165-174 AND 991-1000, CHARACTERIZATION. Strain: ATCC 76621 / YPH252.
[6]	"Identification of two novel TAF subunits of the yeast Saccharomyces cerevisiae TFIID complex." Sanders S.L., Weil P.A. J. Biol. Chem. 275:13895-13900(2000) [PubMed: 10788514] [Abstract] Cited for: FUNCTION, SUBUNIT.
[7]	"Molecular characterization of Saccharomyces cerevisiae TFIID." Sanders S.L., Garbett K.A., Weil P.A. Mol. Cell. Biol. 22:6000-6013(2002) [PubMed: 12138208] [Abstract] Cited for: FUNCTION, TFIID STOICHIOMETRY.
[8]	"Multi-protein complexes in eukaryotic gene transcription." Martinez E. Plant Mol. Biol. 50:925-947(2002) [PubMed: 12516863] [Abstract] Cited for: FUNCTION.
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Mapping histone fold TAFs within yeast TFIID." Leurent C., Sanders S.L., Ruhlmann C., Mallouh V., Weil P.A., Kirschner D.B., Tora L., Schultz P. EMBO J. 21:3424-3433(2002) [PubMed: 12093743] [Abstract] Cited for: 3D-STRUCTURE, ELECTRON MICROSCOPY OF TFIID.
[11]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-318, MASS SPECTROMETRY. Strain: ADR376.
[12]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-268, MASS SPECTROMETRY.
[13]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-19 AND SER-318, MASS SPECTROMETRY.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-18; THR-19; THR-158; THR-161; SER-163; THR-166 AND SER-318, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63853 Genomic DNA. Translation: CAA45337.1. M60486 Genomic DNA. Translation: AAA35179.1. X59720 Genomic DNA. Translation: CAA42290.1. BK006937 Genomic DNA. Translation: DAA07520.1.
PIR	BWBYM1. S19455.
RefSeq	NP_009971.1. NM_001178756.1.
3D structure databases
ProteinModelPortal	P23255.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-343N.
IntAct	P23255. 68 interactions.
MINT	MINT-617656.
STRING	P23255.
Proteomic databases
PeptideAtlas	P23255.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YCR042C; YCR042C; YCR042C.
GeneID	850409.
KEGG	sce:YCR042C.
NMPDR	fig|4932.3.peg.697.
Organism-specific databases
CYGD	YCR042c.
SGD	S000000638. TAF2.
Phylogenomic databases
eggNOG	fuNOG05212.
GeneTree	EFGT00050000002057.
HOGENOM	HBG397545.
OMA	TPYEHIV.
OrthoDB	EOG43JGCZ.
Gene expression databases
ArrayExpress	P23255.
Genevestigator	P23255.
GermOnline	YCR042C. Saccharomyces cerevisiae.
Family and domain databases
KO	K03128.
ProtoNet	Search...
Other
NextBio	965956.

Entry information

Entry name

TAF2_YEAST

Accession

Primary (citable) accession number: P23255
Secondary accession number(s): D6VR51

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	May 1, 1992
Last modified:	December 14, 2011
This is version 109 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program