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Protein

Transcription initiation factor TFIID subunit 2

Gene

TAF2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a component of the DNA-binding general transcription factor complex TFIID. Binding of TFIID to a promoter (with or without TATA element) is the initial step in pre-initiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription.3 Publications

Miscellaneous

Present with 1200 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processTranscription, Transcription regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-29353-MONOMER
ReactomeiR-SCE-674695 RNA Polymerase II Pre-transcription Events
R-SCE-73776 RNA Polymerase II Promoter Escape
R-SCE-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-SCE-75953 RNA Polymerase II Transcription Initiation
R-SCE-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 2
Alternative name(s):
TAFII-150
TBP-associated factor 150 kDa
TBP-associated factor 2
TSM-1
Gene namesi
Name:TAF2
Synonyms:TAF150, TSM1
Ordered Locus Names:YCR042C
ORF Names:YCR42C, YCR724
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome III

Organism-specific databases

EuPathDBiFungiDB:YCR042C
SGDiS000000638 TAF2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188671 – 1407Transcription initiation factor TFIID subunit 2Add BLAST1407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei158PhosphothreonineCombined sources1
Modified residuei161PhosphothreonineCombined sources1
Modified residuei163PhosphoserineCombined sources1
Modified residuei318PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23255
PaxDbiP23255
PRIDEiP23255

PTM databases

iPTMnetiP23255

Interactioni

Subunit structurei

The 1.2 MDa TFIID complex is composed of TATA binding protein (TBP) and the 14 TBP-associated factors. One copy of each TAF1, TAF2, TAF3, TAF7, TAF8, TAF11, TAF13, two copies of each TAF4, TAF5, TAF6, TAF9, TAF10, TAF12, and three copies of TAF14.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi31024, 43 interactors
DIPiDIP-343N
IntActiP23255, 23 interactors
MINTiP23255
STRINGi4932.YCR042C

Structurei

3D structure databases

ProteinModelPortaliP23255
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1285 – 1350Highly chargedAdd BLAST66

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili304 – 337Sequence analysisAdd BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi52 – 55Poly-Ile4
Compositional biasi259 – 334Asp/Glu-rich (acidic)Add BLAST76

Sequence similaritiesi

Belongs to the TAF2 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

GeneTreeiENSGT00390000000420
HOGENOMiHOG000157894
InParanoidiP23255
KOiK03128
OMAiSHGICPP
OrthoDBiEOG092C3V5H

Family and domain databases

InterProiView protein in InterPro
IPR037813 TAF2
PANTHERiPTHR15137 PTHR15137, 1 hit

Sequencei

Sequence statusi: Complete.

P23255-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMSFSKNATP RAIVSESSTL HEMKFRNFRV AHEKISLDID LATHCITGSA
60 70 80 90 100
TIIIIPLIQN LEYVTFDCKE MTIKDVLVEN RRCDQFIHDD PLQTNLNGLT
110 120 130 140 150
SQNVLYSDNS IEQSHFLRSK FASLNEYPET DSKSQLTIKI PSSIKISLED
160 170 180 190 200
ANALSNYTPI TPSIKTTPGF QESVFTPITL QIEYEIRNPK SGIKFDTVYA
210 220 230 240 250
DKPWLWNVYT SNGEICSSAS YWVPCVDLLD EKSTWELEFS VPRLVKNIGT
260 270 280 290 300
SKLIGQNGEE SEKEKEDTPE HDEEEEGKPA RVIKDEDKDS NLKNDEEGKN
310 320 330 340 350
SKSKDAQDND EEEEEGESDE EEEEGEEERR NIEESNNPSL RDVIVCCSEY
360 370 380 390 400
SNIKELPHPI DLTKKKCIFQ IINPVAPHHI GWAIGAFNSW SLPLISPPSV
410 420 430 440 450
DAEDEVEEDK LRENVVDNVN DTMDDDIGSD IIPIQIFTLP TQETDELTVI
460 470 480 490 500
NSTVVCQKII DFYSKEFGSY PFTCYSMVFL PTAPSKHMDF AALGICNTRL
510 520 530 540 550
LYPLEVIDKA FSTTNELAWA LANQWSCVNI TPLDMNDYWC CLGIAGYMVF
560 570 580 590 600
QVTKKLMGNN TYKYQLKRNS EAIVEQDFEK PPIGSTFTGS SRPISWSSKD
610 620 630 640 650
LSFIQLKAPM ILHILDRRMT KTERSFGMSR VLPKIFLQAM SGDLPNNSLT
660 670 680 690 700
SSHFQHVCER VNKSKLENFF NEWVYGSGVP ILRVTQRFNR KRMVIELGIR
710 720 730 740 750
QVQDEELGHE KVVGEEGFFK SALDHLEHPD LNRTECFTGS MTIRIHEHDG
760 770 780 790 800
TPYEHIVEIK DTFTKIDIQY NTKYRRLRKR GGGANDENGV ENNNEEKPIV
810 820 830 840 850
VDVNCLGNVY MSPEECSRFS LTEFNRTSES NELLKQNEAF EWIRIDSDLE
860 870 880 890 900
WICQMHINQP DYMFSSQLRQ DGDIEAQLEA IRYYEDVVVN GGVKSLVYSS
910 920 930 940 950
ILFRTAIDER YFFGIRLAAC EALSKYVYDP DFTGGVKHLI QIFQILFCLE
960 970 980 990 1000
DSNIPKSNNF ENPKLYFLQC NIPKYLAKVK NENGKCPKLV KQFLLDILVY
1010 1020 1030 1040 1050
NENGENKYSD DAYVRSLIEN VVKVALNEYK DKAYMEKVKT QLLRYENLVN
1060 1070 1080 1090 1100
WLSSYESLIK TTIMYAKYKL HKVGAYDFTE LTGMIMHTLT LGINNGDISR
1110 1120 1130 1140 1150
ESFQNEFLMV LKIMLLEGGL KNKDALVLFT EILCFHEDSY IRDKSVDVLS
1160 1170 1180 1190 1200
ECVNLVVMDG SLDTISDDIK SSVQSVHNEV KNIKSEDDIE LFLSGHYVDD
1210 1220 1230 1240 1250
MKIKIEKIGR QNISGLIQIC RDMFKGYSPL KILLWDVLNL PVLSLYQRKQ
1260 1270 1280 1290 1300
IHDLVRVMYT LINSFVVRLE TPRERRLVAK MNSNEEGKLD IVIKRESILK
1310 1320 1330 1340 1350
VHIKKEVTST VEAPKKANKI KISLKGDKPV RKVEKQIVKP KVTSKQRKVK
1360 1370 1380 1390 1400
SHVNRMGSLP LRFVKIQQQP RVMVHLSSVP YSQFVQITKV TSRSFMVKIR

TKNDAKN
Length:1,407
Mass (Da):161,471
Last modified:May 1, 1992 - v3
Checksum:i184C8ED735443F38
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti323E → G in AAA35179 (PubMed:1840512).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63853 Genomic DNA Translation: CAA45337.1
M60486 Genomic DNA Translation: AAA35179.1
X59720 Genomic DNA Translation: CAA42290.1
BK006937 Genomic DNA Translation: DAA07520.1
PIRiS19455 BWBYM1
RefSeqiNP_009971.1, NM_001178756.1

Genome annotation databases

EnsemblFungiiCAA42290; CAA42290; CAA42290
YCR042C; YCR042C; YCR042C
GeneIDi850409
KEGGisce:YCR042C

Similar proteinsi

Entry informationi

Entry nameiTAF2_YEAST
AccessioniPrimary (citable) accession number: P23255
Secondary accession number(s): D6VR51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 1, 1992
Last modified: March 28, 2018
This is version 161 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health