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P23253 (TCNA_TRYCR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase

EC=3.2.1.18
Alternative name(s):
Major surface antigen
Neuraminidase
Short name=NA
Gene names
Name:TCNA
OrganismTrypanosoma cruzi
Taxonomic identifier5693 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum

Protein attributes

Sequence length1162 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Developmentally regulated neuraminidase implicated in parasite invasion of cells.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor Potential Ref.2.

Developmental stage

Maximal activity in trypomastigotes, minimum in epimastigotes and not detectable in amastigotes.

Miscellaneous

The variable lengths of the long tandem repeat domain could account in part for the polymorphism of the TCNA protein.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 3 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11621162Sialidase
PRO_0000208910

Regions

Repeat23 – 3412BNR 1
Repeat163 – 17412BNR 2
Repeat209 – 22012BNR 3
Region589 – 112053244 X 12 AA tandem repeats, LTR domain
Compositional bias1 – 457457Cys-rich

Amino acid modifications

Glycosylation3421N-linked (GlcNAc...) Potential
Glycosylation3941N-linked (GlcNAc...) Potential
Glycosylation11251N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P23253 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 07049221897C6A40

FASTA1,162120,033
        10         20         30         40         50         60 
MVAIADARYE TSSENSLIDT VAKYSVDDGE TWETQIAIKN SRVSSVSRVV DPTVIVKGNK 

        70         80         90        100        110        120 
LYVLVGSYYS SRSYWSSHGD ARDWDILLAV GEVTKSTAGG KITASIKWGS PVSLKKFFPA 

       130        140        150        160        170        180 
EMEGMHTNQF LGGAGVAIVA SNGNLVYPVQ VTNKRKQVFS KIFYSEDDGK TWKFGKGRSD 

       190        200        210        220        230        240 
FGCSEPVALE WEGKLIINTR VDWKRRLVYE SSDMEKPWVE AVGTVSRVWG PSPKSNQPGS 

       250        260        270        280        290        300 
QTSFTAVTIE GMRVMLFTHP LNFKGRCVRD RLNLWLTDNQ RIYNVGQVSI GDENSAYSSV 

       310        320        330        340        350        360 
LYKDDKLYCL HEINTDEVYS LVFARLVGEL RIIKSVLRSW KNWTATCPAF APLLIQPLRR 

       370        380        390        400        410        420 
QRVVVVPLSP RLVLLAFCRQ RLPKRMGGSY RCVNASTANA ERVRNGLKFA GVGGGALWPV 

       430        440        450        460        470        480 
SQQGQNQRYR FANHAFTLVA SVTIHEAPRA ASPLLGASLD SSGGKKLLGL SYDEKHQWQP 

       490        500        510        520        530        540 
IYGSTPVTPT GSWETGKRYH LVLTMANKIG SVYIDGELLE GSGQTVVPDG RTPDISHFYV 

       550        560        570        580        590        600 
GGYKRSDMPT ISHVTVNNVL LYNRRQLNTE EIRTLFLSQD LIGTEAHMDS SSDSSAHSTP 

       610        620        630        640        650        660 
STPADSSAHS TPSTPVDSSA HSTPSTPADS SAHGTPSTPV DSSAHGTPST PADSSAHGTP 

       670        680        690        700        710        720 
STPVDSSAHS TPSTPVDSSA HSTPSTPVDS SAHGAPSTPA DSSAHGTPST PVDSSAHGTP 

       730        740        750        760        770        780 
STPADSSAHS TPSTPADSSA HSTPSTPADS SAHSTPSTPV DSSAHGTPST PADSSAHSTP 

       790        800        810        820        830        840 
STPADSSAHG TPSTPVDSSA HSTPSTPVDS SAHGTPSTPV DSSAHSTPST PVDSSAHGTP 

       850        860        870        880        890        900 
STPVDSSAHS TPSTPADSSA HSTPSTPADS SAHGTPSTPV DSSAHSTPST PADSSAHSTP 

       910        920        930        940        950        960 
STPVDSSAHS TPSTPADSSA HGTPSTPVDS SAHGTPSTPA DSSAHSTPST PADSSAHSTP 

       970        980        990       1000       1010       1020 
STPADSSAHS TPSTPVDSSA HSTPSTPADS SAHSTPSTPA DSSAHSTPST PADSSAHSTP 

      1030       1040       1050       1060       1070       1080 
STPVDSSAHS TPSTPADSSA HGTPSTPADS SAHSTPSTPV DSSAHSTPST PADSSAHGTP 

      1090       1100       1110       1120       1130       1140 
STPADSSAHS TPSTPADSSA HGTPSTPADS SAHSTPSTPA GSSANGTVLI LPDGAALSTF 

      1150       1160 
SGGGLLLCAC ALLLHVFFMA VF 

« Hide

References

[1]"The Trypanosoma cruzi neuraminidase contains sequences similar to bacterial neuraminidases, YWTD repeats of the low density lipoprotein receptor, and type III modules of fibronectin."
Pereira M.E.A., Mejia J.S., Ortega-Barria E., Matzilevich D., Prioli R.P.
J. Exp. Med. 174:179-191(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Silvio X-10/4.
[2]"Trypanosoma cruzi: localization of neuraminidase on the surface of trypomastigotes."
Prioli R.P., Mejia J.S., Aji T., Aikawa M., Pereira M.E.A.
Trop. Med. Parasitol. 42:146-150(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M61732 Genomic DNA. Translation: AAA30255.1.
PIRJH0557.

3D structure databases

ProteinModelPortalP23253.
SMRP23253. Positions 1-588.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.120.10.10. 1 hit.
2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF5. PTHR10628:SF5. 1 hit.
PRINTSPR01803. TCSIALIDASE.
SUPFAMSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTCNA_TRYCR
AccessionPrimary (citable) accession number: P23253
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries