P23253 (TCNA_TRYCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sialidase
EC=3.2.1.18

Alternative name(s):
Major surface antigen
Neuraminidase
Short name=NA

Gene names

Name:

TCNA

Organism

Trypanosoma cruzi

Taxonomic identifier

5693 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma › Schizotrypanum

Protein attributes

Sequence length	1162 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Developmentally regulated neuraminidase implicated in parasite invasion of cells.
Catalytic activity	Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor Potential Ref.2.
Developmental stage	Maximal activity in trypomastigotes, minimum in epimastigotes and not detectable in amastigotes.
Miscellaneous	The variable lengths of the long tandem repeat domain could account in part for the polymorphism of the TCNA protein.
Sequence similarities	Belongs to the glycosyl hydrolase 33 family. Contains 3 BNR repeats.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat
Molecular function	Glycosidase Hydrolase
PTM	GPI-anchor Glycoprotein Lipoprotein Phosphoprotein
Gene Ontology (GO)
Biological_process	pathogenesis Inferred from electronic annotation. Source: InterPro
Cellular_component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	exo-alpha-(2->3)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->6)-sialidase activity Inferred from electronic annotation. Source: EC exo-alpha-(2->8)-sialidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1162	1162	Sialidase		PRO_0000208910
Regions
	Repeat	23 – 34	12	BNR 1
	Repeat	163 – 174	12	BNR 2
	Repeat	209 – 220	12	BNR 3
	Region	589 – 1120	532	44 X 12 AA tandem repeats, LTR domain
	Compositional bias	1 – 457	457	Cys-rich
Amino acid modifications
	Glycosylation	342	1	N-linked (GlcNAc...) Potential
	Glycosylation	394	1	N-linked (GlcNAc...) Potential
	Glycosylation	1125	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P23253 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 07049221897C6A40	FASTA	1,162	120,033
	10 20 30 40 50 60 MVAIADARYE TSSENSLIDT VAKYSVDDGE TWETQIAIKN SRVSSVSRVV DPTVIVKGNK 70 80 90 100 110 120 LYVLVGSYYS SRSYWSSHGD ARDWDILLAV GEVTKSTAGG KITASIKWGS PVSLKKFFPA 130 140 150 160 170 180 EMEGMHTNQF LGGAGVAIVA SNGNLVYPVQ VTNKRKQVFS KIFYSEDDGK TWKFGKGRSD 190 200 210 220 230 240 FGCSEPVALE WEGKLIINTR VDWKRRLVYE SSDMEKPWVE AVGTVSRVWG PSPKSNQPGS 250 260 270 280 290 300 QTSFTAVTIE GMRVMLFTHP LNFKGRCVRD RLNLWLTDNQ RIYNVGQVSI GDENSAYSSV 310 320 330 340 350 360 LYKDDKLYCL HEINTDEVYS LVFARLVGEL RIIKSVLRSW KNWTATCPAF APLLIQPLRR 370 380 390 400 410 420 QRVVVVPLSP RLVLLAFCRQ RLPKRMGGSY RCVNASTANA ERVRNGLKFA GVGGGALWPV 430 440 450 460 470 480 SQQGQNQRYR FANHAFTLVA SVTIHEAPRA ASPLLGASLD SSGGKKLLGL SYDEKHQWQP 490 500 510 520 530 540 IYGSTPVTPT GSWETGKRYH LVLTMANKIG SVYIDGELLE GSGQTVVPDG RTPDISHFYV 550 560 570 580 590 600 GGYKRSDMPT ISHVTVNNVL LYNRRQLNTE EIRTLFLSQD LIGTEAHMDS SSDSSAHSTP 610 620 630 640 650 660 STPADSSAHS TPSTPVDSSA HSTPSTPADS SAHGTPSTPV DSSAHGTPST PADSSAHGTP 670 680 690 700 710 720 STPVDSSAHS TPSTPVDSSA HSTPSTPVDS SAHGAPSTPA DSSAHGTPST PVDSSAHGTP 730 740 750 760 770 780 STPADSSAHS TPSTPADSSA HSTPSTPADS SAHSTPSTPV DSSAHGTPST PADSSAHSTP 790 800 810 820 830 840 STPADSSAHG TPSTPVDSSA HSTPSTPVDS SAHGTPSTPV DSSAHSTPST PVDSSAHGTP 850 860 870 880 890 900 STPVDSSAHS TPSTPADSSA HSTPSTPADS SAHGTPSTPV DSSAHSTPST PADSSAHSTP 910 920 930 940 950 960 STPVDSSAHS TPSTPADSSA HGTPSTPVDS SAHGTPSTPA DSSAHSTPST PADSSAHSTP 970 980 990 1000 1010 1020 STPADSSAHS TPSTPVDSSA HSTPSTPADS SAHSTPSTPA DSSAHSTPST PADSSAHSTP 1030 1040 1050 1060 1070 1080 STPVDSSAHS TPSTPADSSA HGTPSTPADS SAHSTPSTPV DSSAHSTPST PADSSAHGTP 1090 1100 1110 1120 1130 1140 STPADSSAHS TPSTPADSSA HGTPSTPADS SAHSTPSTPA GSSANGTVLI LPDGAALSTF 1150 1160 SGGGLLLCAC ALLLHVFFMA VF

References

[1]	"The Trypanosoma cruzi neuraminidase contains sequences similar to bacterial neuraminidases, YWTD repeats of the low density lipoprotein receptor, and type III modules of fibronectin." Pereira M.E.A., Mejia J.S., Ortega-Barria E., Matzilevich D., Prioli R.P. J. Exp. Med. 174:179-191(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Silvio X-10/4.
[2]	"Trypanosoma cruzi: localization of neuraminidase on the surface of trypomastigotes." Prioli R.P., Mejia J.S., Aji T., Aikawa M., Pereira M.E.A. Trop. Med. Parasitol. 42:146-150(1991) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M61732 Genomic DNA. Translation: AAA30255.1.
PIR	JH0557.
3D structure databases
ProteinModelPortal	P23253.
SMR	P23253. Positions 1-588.
ModBase	Search...
Protein family/group databases
CAZy	GH33. Glycoside Hydrolase Family 33.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.120.10.10. 1 hit. 2.60.120.200. 1 hit.
InterPro	IPR008985. ConA-like_lec_gl_sf. IPR013320. ConA-like_subgrp. IPR008377. Sialidase_trypan. IPR011040. Sialidases. [Graphical view]
PRINTS	PR01803. TCSIALIDASE.
SUPFAM	SSF49899. ConA_like_lec_gl. 1 hit. SSF50939. Sialidase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TCNA_TRYCR

Accession

Primary (citable) accession number: P23253

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)