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Protein

Sialidase

Gene

TCNA

Organism
Trypanosoma cruzi
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Developmentally regulated neuraminidase implicated in parasite invasion of cells.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase (EC:3.2.1.18)
Alternative name(s):
Major surface antigen
Neuraminidase
Short name:
NA
Gene namesi
Name:TCNA
OrganismiTrypanosoma cruzi
Taxonomic identifieri5693 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosomaSchizotrypanum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11621162SialidasePRO_0000208910Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi342 – 3421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi394 – 3941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi1125 – 11251N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Expressioni

Developmental stagei

Maximal activity in trypomastigotes, minimum in epimastigotes and not detectable in amastigotes.

Structurei

3D structure databases

ProteinModelPortaliP23253.
SMRiP23253. Positions 1-588.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati23 – 3412BNR 1Add
BLAST
Repeati163 – 17412BNR 2Add
BLAST
Repeati209 – 22012BNR 3Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni589 – 112053244 X 12 AA tandem repeats, LTR domainAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1 – 457457Cys-richAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 3 BNR repeats.Curated

Keywords - Domaini

Repeat

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF5. PTHR10628:SF5. 1 hit.
PRINTSiPR01803. TCSIALIDASE.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

P23253-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVAIADARYE TSSENSLIDT VAKYSVDDGE TWETQIAIKN SRVSSVSRVV
60 70 80 90 100
DPTVIVKGNK LYVLVGSYYS SRSYWSSHGD ARDWDILLAV GEVTKSTAGG
110 120 130 140 150
KITASIKWGS PVSLKKFFPA EMEGMHTNQF LGGAGVAIVA SNGNLVYPVQ
160 170 180 190 200
VTNKRKQVFS KIFYSEDDGK TWKFGKGRSD FGCSEPVALE WEGKLIINTR
210 220 230 240 250
VDWKRRLVYE SSDMEKPWVE AVGTVSRVWG PSPKSNQPGS QTSFTAVTIE
260 270 280 290 300
GMRVMLFTHP LNFKGRCVRD RLNLWLTDNQ RIYNVGQVSI GDENSAYSSV
310 320 330 340 350
LYKDDKLYCL HEINTDEVYS LVFARLVGEL RIIKSVLRSW KNWTATCPAF
360 370 380 390 400
APLLIQPLRR QRVVVVPLSP RLVLLAFCRQ RLPKRMGGSY RCVNASTANA
410 420 430 440 450
ERVRNGLKFA GVGGGALWPV SQQGQNQRYR FANHAFTLVA SVTIHEAPRA
460 470 480 490 500
ASPLLGASLD SSGGKKLLGL SYDEKHQWQP IYGSTPVTPT GSWETGKRYH
510 520 530 540 550
LVLTMANKIG SVYIDGELLE GSGQTVVPDG RTPDISHFYV GGYKRSDMPT
560 570 580 590 600
ISHVTVNNVL LYNRRQLNTE EIRTLFLSQD LIGTEAHMDS SSDSSAHSTP
610 620 630 640 650
STPADSSAHS TPSTPVDSSA HSTPSTPADS SAHGTPSTPV DSSAHGTPST
660 670 680 690 700
PADSSAHGTP STPVDSSAHS TPSTPVDSSA HSTPSTPVDS SAHGAPSTPA
710 720 730 740 750
DSSAHGTPST PVDSSAHGTP STPADSSAHS TPSTPADSSA HSTPSTPADS
760 770 780 790 800
SAHSTPSTPV DSSAHGTPST PADSSAHSTP STPADSSAHG TPSTPVDSSA
810 820 830 840 850
HSTPSTPVDS SAHGTPSTPV DSSAHSTPST PVDSSAHGTP STPVDSSAHS
860 870 880 890 900
TPSTPADSSA HSTPSTPADS SAHGTPSTPV DSSAHSTPST PADSSAHSTP
910 920 930 940 950
STPVDSSAHS TPSTPADSSA HGTPSTPVDS SAHGTPSTPA DSSAHSTPST
960 970 980 990 1000
PADSSAHSTP STPADSSAHS TPSTPVDSSA HSTPSTPADS SAHSTPSTPA
1010 1020 1030 1040 1050
DSSAHSTPST PADSSAHSTP STPVDSSAHS TPSTPADSSA HGTPSTPADS
1060 1070 1080 1090 1100
SAHSTPSTPV DSSAHSTPST PADSSAHGTP STPADSSAHS TPSTPADSSA
1110 1120 1130 1140 1150
HGTPSTPADS SAHSTPSTPA GSSANGTVLI LPDGAALSTF SGGGLLLCAC
1160
ALLLHVFFMA VF
Length:1,162
Mass (Da):120,033
Last modified:November 1, 1991 - v1
Checksum:i07049221897C6A40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61732 Genomic DNA. Translation: AAA30255.1.
PIRiJH0557.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61732 Genomic DNA. Translation: AAA30255.1.
PIRiJH0557.

3D structure databases

ProteinModelPortaliP23253.
SMRiP23253. Positions 1-588.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF5. PTHR10628:SF5. 1 hit.
PRINTSiPR01803. TCSIALIDASE.
SUPFAMiSSF49899. SSF49899. 1 hit.
SSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The Trypanosoma cruzi neuraminidase contains sequences similar to bacterial neuraminidases, YWTD repeats of the low density lipoprotein receptor, and type III modules of fibronectin."
    Pereira M.E.A., Mejia J.S., Ortega-Barria E., Matzilevich D., Prioli R.P.
    J. Exp. Med. 174:179-191(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Silvio X-10/4.
  2. "Trypanosoma cruzi: localization of neuraminidase on the surface of trypomastigotes."
    Prioli R.P., Mejia J.S., Aji T., Aikawa M., Pereira M.E.A.
    Trop. Med. Parasitol. 42:146-150(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiTCNA_TRYCR
AccessioniPrimary (citable) accession number: P23253
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

The variable lengths of the long tandem repeat domain could account in part for the polymorphism of the TCNA protein.

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.