Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P23248

- RS3A2_YEAST

UniProt

P23248 - RS3A2_YEAST

Protein

40S ribosomal protein S1-B

Gene

RPS1B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    GO - Molecular functioni

    1. structural constituent of ribosome Source: SGD

    GO - Biological processi

    1. cytoplasmic translation Source: SGD
    2. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

    Keywords - Molecular functioni

    Ribonucleoprotein, Ribosomal protein

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32658-MONOMER.
    ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    40S ribosomal protein S1-BUniRule annotation
    Alternative name(s):
    RP10B
    Gene namesi
    Name:RPS1BUniRule annotation
    Synonyms:PLC2, RPS10B
    Ordered Locus Names:YML063W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    SGDiS000004528. RPS1B.

    Subcellular locationi

    Cytoplasm 1 PublicationUniRule annotation

    GO - Cellular componenti

    1. cytosolic small ribosomal subunit Source: SGD

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 PublicationUniRule annotation
    Chaini2 – 25525440S ribosomal protein S1-BPRO_0000153542Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine; partialUniRule annotation
    Modified residuei245 – 2451Phosphoserine3 Publications
    Modified residuei254 – 2541Phosphothreonine2 Publications

    Post-translational modificationi

    N-terminally acetylated by acetyltransferase NatA.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP23248.
    PeptideAtlasiP23248.

    Expressioni

    Gene expression databases

    GenevestigatoriP23248.

    Interactioni

    Subunit structurei

    Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

    Protein-protein interaction databases

    BioGridi35103. 156 interactions.
    IntActiP23248. 74 interactions.
    MINTiMINT-4496899.
    STRINGi4932.YML063W.

    Structurei

    3D structure databases

    ProteinModelPortaliP23248.
    SMRiP23248. Positions 20-235.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribosomal protein S3Ae family.UniRule annotation

    Phylogenomic databases

    GeneTreeiENSGT00390000018433.
    HOGENOMiHOG000105220.
    KOiK02984.
    OMAiVHKAKIL.
    OrthoDBiEOG7QVMDJ.

    Family and domain databases

    HAMAPiMF_03122. Ribosomal_S3Ae_euk.
    InterProiIPR027500. Ribosomal_S1/3_euk.
    IPR001593. Ribosomal_S3Ae.
    IPR018281. Ribosomal_S3Ae_CS.
    [Graphical view]
    PfamiPF01015. Ribosomal_S3Ae. 1 hit.
    [Graphical view]
    PROSITEiPS01191. RIBOSOMAL_S3AE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23248-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVGKNKRLS RGKKGLKKKV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK    50
    STGLKNASDA LKGRVVEVCL ADLQGSEDHS FRKVKLRVDE VQGKNLLTNF 100
    HGMDFTTDKL RSMVRKWQTL IEANVTVKTS DDYVLRIFAI AFTRKQANQV 150
    KRHSYAQSSH IRAIRKVISE ILTREVQNST LAQLTSKLIP EVINKEIENA 200
    TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK GKKVSGFKDE 250
    VLETV 255
    Length:255
    Mass (Da):28,812
    Last modified:January 23, 2007 - v3
    Checksum:i192A913E3AD5C7DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55360 Genomic DNA. Translation: CAA39044.1.
    Z38114 Genomic DNA. Translation: CAA86258.1.
    AY557981 Genomic DNA. Translation: AAS56307.1.
    BK006946 Genomic DNA. Translation: DAA09834.1.
    PIRiS14051.
    RefSeqiNP_013648.1. NM_001182422.1.

    Genome annotation databases

    EnsemblFungiiYML063W; YML063W; YML063W.
    GeneIDi854939.
    KEGGisce:YML063W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55360 Genomic DNA. Translation: CAA39044.1 .
    Z38114 Genomic DNA. Translation: CAA86258.1 .
    AY557981 Genomic DNA. Translation: AAS56307.1 .
    BK006946 Genomic DNA. Translation: DAA09834.1 .
    PIRi S14051.
    RefSeqi NP_013648.1. NM_001182422.1.

    3D structure databases

    ProteinModelPortali P23248.
    SMRi P23248. Positions 20-235.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35103. 156 interactions.
    IntActi P23248. 74 interactions.
    MINTi MINT-4496899.
    STRINGi 4932.YML063W.

    Proteomic databases

    MaxQBi P23248.
    PeptideAtlasi P23248.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YML063W ; YML063W ; YML063W .
    GeneIDi 854939.
    KEGGi sce:YML063W.

    Organism-specific databases

    SGDi S000004528. RPS1B.

    Phylogenomic databases

    GeneTreei ENSGT00390000018433.
    HOGENOMi HOG000105220.
    KOi K02984.
    OMAi VHKAKIL.
    OrthoDBi EOG7QVMDJ.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32658-MONOMER.
    Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
    REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
    REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
    REACT_217188. Formation of a pool of free 40S subunits.

    Miscellaneous databases

    NextBioi 977986.

    Gene expression databases

    Genevestigatori P23248.

    Family and domain databases

    HAMAPi MF_03122. Ribosomal_S3Ae_euk.
    InterProi IPR027500. Ribosomal_S1/3_euk.
    IPR001593. Ribosomal_S3Ae.
    IPR018281. Ribosomal_S3Ae_CS.
    [Graphical view ]
    Pfami PF01015. Ribosomal_S3Ae. 1 hit.
    [Graphical view ]
    PROSITEi PS01191. RIBOSOMAL_S3AE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene."
      Garrett J.M., Singh K.K., Vonder Haar R.A., Emr S.D.
      Mol. Gen. Genet. 225:483-491(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SEY2102.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
      Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
      J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-21.
    6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
      Planta R.J., Mager W.H.
      Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE, SUBUNIT.
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRS3A2_YEAST
    AccessioniPrimary (citable) accession number: P23248
    Secondary accession number(s): D6VZB0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 124 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 107000 molecules/cell in log phase SD medium.1 Publication
    There are 2 genes for S1 in yeast.

    Caution

    Was originally thought to be MFT1, the mitochondrial fusion target protein.1 Publication

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Ribosomal proteins
      Ribosomal proteins families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3