P23248 (RS3A2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
40S ribosomal protein S1-B

Alternative name(s):
RP10B

Gene names

Name:	RPS1B
Synonyms:	PLC2, RPS10B
Ordered Locus Names:	YML063W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6
Subcellular location	Cytoplasm Ref.7.
Post-translational modification	N-terminally acetylated by acetyltransferase NatA By similarity. HAMAP-Rule MF_03122
Miscellaneous	Present with 107000 molecules/cell in log phase SD medium. HAMAP-Rule MF_03122 There are 2 genes for S1 in yeast. HAMAP-Rule MF_03122
Sequence similarities	Belongs to the ribosomal protein S3Ae family.
Caution	Was originally (Ref.1) thought to be MFT1, the mitochondrial fusion target protein.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Ribonucleoprotein Ribosomal protein
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytoplasmic translation Non-traceable author statement Ref.6. Source: SGD maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Inferred from genetic interaction PubMed 16246728. Source: SGD
Cellular_component	90S preribosome Inferred from direct assay PubMed 12150911. Source: SGD cytosolic small ribosomal subunit Non-traceable author statement Ref.6. Source: SGD
Molecular_function	structural constituent of ribosome Non-traceable author statement Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.5
	Chain	2 – 255	254	40S ribosomal protein S1-B HAMAP-Rule MF_03122		PRO_0000153542
Amino acid modifications
	Modified residue	2	1	N-acetylalanine; partial By similarity
	Modified residue	76	1	Phosphoserine Ref.9
	Modified residue	80	1	Phosphoserine Ref.11
	Modified residue	154	1	Phosphoserine Ref.11
	Modified residue	159	1	Phosphoserine Ref.11
	Modified residue	245	1	Phosphoserine Ref.10 Ref.12 Ref.13
	Modified residue	254	1	Phosphothreonine Ref.10 Ref.13

Sequences

	Sequence		Length	Mass (Da)
	P23248 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 192A913E3AD5C7DE	FASTA	255	28,812
	10 20 30 40 50 60 MAVGKNKRLS RGKKGLKKKV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKNASDA 70 80 90 100 110 120 LKGRVVEVCL ADLQGSEDHS FRKVKLRVDE VQGKNLLTNF HGMDFTTDKL RSMVRKWQTL 130 140 150 160 170 180 IEANVTVKTS DDYVLRIFAI AFTRKQANQV KRHSYAQSSH IRAIRKVISE ILTREVQNST 190 200 210 220 230 240 LAQLTSKLIP EVINKEIENA TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK 250 GKKVSGFKDE VLETV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene." Garrett J.M., Singh K.K., Vonder Haar R.A., Emr S.D. Mol. Gen. Genet. 225:483-491(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SEY2102.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae." Takakura H., Tsunasawa S., Miyagi M., Warner J.R. J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-21.
[6]	"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae." Planta R.J., Mager W.H. Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NOMENCLATURE, SUBUNIT.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-76, MASS SPECTROMETRY. Strain: YAL6B.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80; SER-154 AND SER-159, MASS SPECTROMETRY.
[12]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, MASS SPECTROMETRY.
[13]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55360 Genomic DNA. Translation: CAA39044.1. Z38114 Genomic DNA. Translation: CAA86258.1. AY557981 Genomic DNA. Translation: AAS56307.1. BK006946 Genomic DNA. Translation: DAA09834.1.
PIR	S14051.
RefSeq	NP_013648.1. NM_001182422.1.
3D structure databases
ProteinModelPortal	P23248.
SMR	P23248. Positions 20-235.
ModBase	Search...
Protein-protein interaction databases
IntAct	P23248. 71 interactions.
MINT	MINT-4496899.
STRING	4932.YML063W.
Proteomic databases
PeptideAtlas	P23248.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YML063W; YML063W; YML063W.
GeneID	854939.
KEGG	sce:YML063W.
Organism-specific databases
SGD	S000004528. RPS1B.
Phylogenomic databases
GeneTree	ENSGT00390000018433.
HOGENOM	HOG000105220.
KO	K02984.
OMA	GHDTTRE.
OrthoDB	EOG4WM83S.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32658-MONOMER.
Gene expression databases
Genevestigator	P23248.
GermOnline	YML063W. Saccharomyces cerevisiae.
Family and domain databases
HAMAP	MF_03122. Ribosomal_S3Ae_euk.
InterPro	IPR027500. Ribosomal_S1/3_euk. IPR001593. Ribosomal_S3Ae. IPR018281. Ribosomal_S3Ae_CS. [Graphical view]
Pfam	PF01015. Ribosomal_S3Ae. 1 hit. [Graphical view]
PROSITE	PS01191. RIBOSOMAL_S3AE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	977986.

Entry information

Entry name

RS3A2_YEAST

Accession

Primary (citable) accession number: P23248
Secondary accession number(s): D6VZB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 115 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program