Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P23248

- RS3A2_YEAST

UniProt

P23248 - RS3A2_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

40S ribosomal protein S1-B

Gene

RPS1B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  1. structural constituent of ribosome Source: SGD

GO - Biological processi

  1. cytoplasmic translation Source: SGD
  2. maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32658-MONOMER.
ReactomeiREACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S1-BUniRule annotation
Alternative name(s):
RP10B
Gene namesi
Name:RPS1BUniRule annotation
Synonyms:PLC2, RPS10B
Ordered Locus Names:YML063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004528. RPS1B.

Subcellular locationi

Cytoplasm 1 PublicationUniRule annotation

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 PublicationUniRule annotation
Chaini2 – 25525440S ribosomal protein S1-BPRO_0000153542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine; partialUniRule annotation
Modified residuei245 – 2451Phosphoserine3 Publications
Modified residuei254 – 2541Phosphothreonine2 Publications

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP23248.
PeptideAtlasiP23248.

Expressioni

Gene expression databases

GenevestigatoriP23248.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi35103. 157 interactions.
IntActiP23248. 74 interactions.
MINTiMINT-4496899.
STRINGi4932.YML063W.

Structurei

3D structure databases

ProteinModelPortaliP23248.
SMRiP23248. Positions 20-235.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S3Ae family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000018433.
HOGENOMiHOG000105220.
InParanoidiP23248.
KOiK02984.
OMAiVHKAKIL.
OrthoDBiEOG7QVMDJ.

Family and domain databases

HAMAPiMF_03122. Ribosomal_S3Ae_euk.
InterProiIPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
[Graphical view]
PfamiPF01015. Ribosomal_S3Ae. 1 hit.
[Graphical view]
PROSITEiPS01191. RIBOSOMAL_S3AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23248-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVGKNKRLS RGKKGLKKKV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK
60 70 80 90 100
STGLKNASDA LKGRVVEVCL ADLQGSEDHS FRKVKLRVDE VQGKNLLTNF
110 120 130 140 150
HGMDFTTDKL RSMVRKWQTL IEANVTVKTS DDYVLRIFAI AFTRKQANQV
160 170 180 190 200
KRHSYAQSSH IRAIRKVISE ILTREVQNST LAQLTSKLIP EVINKEIENA
210 220 230 240 250
TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK GKKVSGFKDE

VLETV
Length:255
Mass (Da):28,812
Last modified:January 23, 2007 - v3
Checksum:i192A913E3AD5C7DE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55360 Genomic DNA. Translation: CAA39044.1.
Z38114 Genomic DNA. Translation: CAA86258.1.
AY557981 Genomic DNA. Translation: AAS56307.1.
BK006946 Genomic DNA. Translation: DAA09834.1.
PIRiS14051.
RefSeqiNP_013648.1. NM_001182422.1.

Genome annotation databases

EnsemblFungiiYML063W; YML063W; YML063W.
GeneIDi854939.
KEGGisce:YML063W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55360 Genomic DNA. Translation: CAA39044.1 .
Z38114 Genomic DNA. Translation: CAA86258.1 .
AY557981 Genomic DNA. Translation: AAS56307.1 .
BK006946 Genomic DNA. Translation: DAA09834.1 .
PIRi S14051.
RefSeqi NP_013648.1. NM_001182422.1.

3D structure databases

ProteinModelPortali P23248.
SMRi P23248. Positions 20-235.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35103. 157 interactions.
IntActi P23248. 74 interactions.
MINTi MINT-4496899.
STRINGi 4932.YML063W.

Proteomic databases

MaxQBi P23248.
PeptideAtlasi P23248.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YML063W ; YML063W ; YML063W .
GeneIDi 854939.
KEGGi sce:YML063W.

Organism-specific databases

SGDi S000004528. RPS1B.

Phylogenomic databases

GeneTreei ENSGT00390000018433.
HOGENOMi HOG000105220.
InParanoidi P23248.
KOi K02984.
OMAi VHKAKIL.
OrthoDBi EOG7QVMDJ.

Enzyme and pathway databases

BioCyci YEAST:G3O-32658-MONOMER.
Reactomei REACT_188965. SRP-dependent cotranslational protein targeting to membrane.
REACT_189050. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_189183. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_217188. Formation of a pool of free 40S subunits.

Miscellaneous databases

NextBioi 977986.

Gene expression databases

Genevestigatori P23248.

Family and domain databases

HAMAPi MF_03122. Ribosomal_S3Ae_euk.
InterProi IPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
[Graphical view ]
Pfami PF01015. Ribosomal_S3Ae. 1 hit.
[Graphical view ]
PROSITEi PS01191. RIBOSOMAL_S3AE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene."
    Garrett J.M., Singh K.K., Vonder Haar R.A., Emr S.D.
    Mol. Gen. Genet. 225:483-491(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SEY2102.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRS3A2_YEAST
AccessioniPrimary (citable) accession number: P23248
Secondary accession number(s): D6VZB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 107000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S1 in yeast.

Caution

Was originally thought to be MFT1, the mitochondrial fusion target protein.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3