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Protein

40S ribosomal protein S1-B

Gene

RPS1B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 107000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for eS1 in yeast.Curated

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32658-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S1-B1 PublicationUniRule annotation
Alternative name(s):
RP10B
Small ribosomal subunit protein eS1-B1 Publication
Gene namesi
Name:RPS1B1 PublicationUniRule annotation
Synonyms:PLC2, RPS10B
Ordered Locus Names:YML063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML063W.
SGDiS000004528. RPS1B.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central
  • cytosolic small ribosomal subunit Source: SGD

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation1 Publication
ChainiPRO_00001535422 – 25540S ribosomal protein S1-BAdd BLAST254

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine; partialUniRule annotationBy similarity1
Modified residuei245PhosphoserineCombined sources1
Cross-linki248Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei254PhosphothreonineCombined sources1

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP23248.
PRIDEiP23248.
TopDownProteomicsiP23248.

PTM databases

iPTMnetiP23248.

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes). eS1 interacts directly with uS11 and eS26, which form part of the mRNA exit tunnel (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi35103. 223 interactors.
IntActiP23248. 75 interactors.
MINTiMINT-4496899.

Structurei

3D structure databases

ProteinModelPortaliP23248.
SMRiP23248.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the eukaryotic ribosomal protein eS1 family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000018433.
HOGENOMiHOG000105220.
InParanoidiP23248.
KOiK02984.
OMAiMFEIMTR.
OrthoDBiEOG092C4C2Y.

Family and domain databases

HAMAPiMF_03122. Ribosomal_S3Ae_euk. 1 hit.
InterProiView protein in InterPro
IPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
PANTHERiPTHR11830:SF11. PTHR11830:SF11. 1 hit.
PfamiView protein in Pfam
PF01015. Ribosomal_S3Ae. 1 hit.
SMARTiView protein in SMART
SM01397. Ribosomal_S3Ae. 1 hit.
PROSITEiView protein in PROSITE
PS01191. RIBOSOMAL_S3AE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVGKNKRLS RGKKGLKKKV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK
60 70 80 90 100
STGLKNASDA LKGRVVEVCL ADLQGSEDHS FRKVKLRVDE VQGKNLLTNF
110 120 130 140 150
HGMDFTTDKL RSMVRKWQTL IEANVTVKTS DDYVLRIFAI AFTRKQANQV
160 170 180 190 200
KRHSYAQSSH IRAIRKVISE ILTREVQNST LAQLTSKLIP EVINKEIENA
210 220 230 240 250
TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK GKKVSGFKDE

VLETV
Length:255
Mass (Da):28,812
Last modified:January 23, 2007 - v3
Checksum:i192A913E3AD5C7DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55360 Genomic DNA. Translation: CAA39044.1.
Z38114 Genomic DNA. Translation: CAA86258.1.
AY557981 Genomic DNA. Translation: AAS56307.1.
BK006946 Genomic DNA. Translation: DAA09834.1.
PIRiS14051.
RefSeqiNP_013648.1. NM_001182422.1.

Genome annotation databases

EnsemblFungiiYML063W; YML063W; YML063W.
GeneIDi854939.
KEGGisce:YML063W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55360 Genomic DNA. Translation: CAA39044.1.
Z38114 Genomic DNA. Translation: CAA86258.1.
AY557981 Genomic DNA. Translation: AAS56307.1.
BK006946 Genomic DNA. Translation: DAA09834.1.
PIRiS14051.
RefSeqiNP_013648.1. NM_001182422.1.

3D structure databases

ProteinModelPortaliP23248.
SMRiP23248.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35103. 223 interactors.
IntActiP23248. 75 interactors.
MINTiMINT-4496899.

PTM databases

iPTMnetiP23248.

Proteomic databases

MaxQBiP23248.
PRIDEiP23248.
TopDownProteomicsiP23248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML063W; YML063W; YML063W.
GeneIDi854939.
KEGGisce:YML063W.

Organism-specific databases

EuPathDBiFungiDB:YML063W.
SGDiS000004528. RPS1B.

Phylogenomic databases

GeneTreeiENSGT00390000018433.
HOGENOMiHOG000105220.
InParanoidiP23248.
KOiK02984.
OMAiMFEIMTR.
OrthoDBiEOG092C4C2Y.

Enzyme and pathway databases

BioCyciYEAST:G3O-32658-MONOMER.
ReactomeiR-SCE-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SCE-1799339. SRP-dependent cotranslational protein targeting to membrane.
R-SCE-72689. Formation of a pool of free 40S subunits.
R-SCE-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-SCE-72702. Ribosomal scanning and start codon recognition.
R-SCE-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
R-SCE-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SCE-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

PROiPR:P23248.

Family and domain databases

HAMAPiMF_03122. Ribosomal_S3Ae_euk. 1 hit.
InterProiView protein in InterPro
IPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
PANTHERiPTHR11830:SF11. PTHR11830:SF11. 1 hit.
PfamiView protein in Pfam
PF01015. Ribosomal_S3Ae. 1 hit.
SMARTiView protein in SMART
SM01397. Ribosomal_S3Ae. 1 hit.
PROSITEiView protein in PROSITE
PS01191. RIBOSOMAL_S3AE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRS3A2_YEAST
AccessioniPrimary (citable) accession number: P23248
Secondary accession number(s): D6VZB0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 150 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be MFT1, the mitochondrial fusion target protein.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.