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P23248 (RS3A2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
40S ribosomal protein S1-B
Alternative name(s):
RP10B
Gene names
Name:RPS1B
Synonyms:PLC2, RPS10B
Ordered Locus Names:YML063W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length255 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S). Ref.6

Subcellular location

Cytoplasm Ref.7.

Post-translational modification

N-terminally acetylated by acetyltransferase NatA By similarity. HAMAP-Rule MF_03122

Miscellaneous

Present with 107000 molecules/cell in log phase SD medium.

There are 2 genes for S1 in yeast.

Sequence similarities

Belongs to the ribosomal protein S3Ae family.

Caution

Was originally (Ref.1) thought to be MFT1, the mitochondrial fusion target protein.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 25525440S ribosomal protein S1-B HAMAP-Rule MF_03122
PRO_0000153542

Amino acid modifications

Modified residue21N-acetylalanine; partial By similarity
Modified residue2451Phosphoserine Ref.9 Ref.10 Ref.11
Modified residue2541Phosphothreonine Ref.9 Ref.10

Sequences

Sequence LengthMass (Da)Tools
P23248 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 192A913E3AD5C7DE

FASTA25528,812
        10         20         30         40         50         60 
MAVGKNKRLS RGKKGLKKKV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK STGLKNASDA 

        70         80         90        100        110        120 
LKGRVVEVCL ADLQGSEDHS FRKVKLRVDE VQGKNLLTNF HGMDFTTDKL RSMVRKWQTL 

       130        140        150        160        170        180 
IEANVTVKTS DDYVLRIFAI AFTRKQANQV KRHSYAQSSH IRAIRKVISE ILTREVQNST 

       190        200        210        220        230        240 
LAQLTSKLIP EVINKEIENA TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK 

       250 
GKKVSGFKDE VLETV 

« Hide

References

« Hide 'large scale' references
[1]"Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene."
Garrett J.M., Singh K.K., Vonder Haar R.A., Emr S.D.
Mol. Gen. Genet. 225:483-491(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SEY2102.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-21.
[6]"The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
Planta R.J., Mager W.H.
Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NOMENCLATURE, SUBUNIT.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55360 Genomic DNA. Translation: CAA39044.1.
Z38114 Genomic DNA. Translation: CAA86258.1.
AY557981 Genomic DNA. Translation: AAS56307.1.
BK006946 Genomic DNA. Translation: DAA09834.1.
PIRS14051.
RefSeqNP_013648.1. NM_001182422.1.

3D structure databases

ProteinModelPortalP23248.
SMRP23248. Positions 20-235.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35103. 155 interactions.
IntActP23248. 74 interactions.
MINTMINT-4496899.
STRING4932.YML063W.

Proteomic databases

PeptideAtlasP23248.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYML063W; YML063W; YML063W.
GeneID854939.
KEGGsce:YML063W.

Organism-specific databases

SGDS000004528. RPS1B.

Phylogenomic databases

GeneTreeENSGT00390000018433.
HOGENOMHOG000105220.
KOK02984.
OMAITKDISH.
OrthoDBEOG7QVMDJ.

Enzyme and pathway databases

BioCycYEAST:G3O-32658-MONOMER.

Gene expression databases

GenevestigatorP23248.

Family and domain databases

HAMAPMF_03122. Ribosomal_S3Ae_euk.
InterProIPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
[Graphical view]
PfamPF01015. Ribosomal_S3Ae. 1 hit.
[Graphical view]
PROSITEPS01191. RIBOSOMAL_S3AE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio977986.

Entry information

Entry nameRS3A2_YEAST
AccessionPrimary (citable) accession number: P23248
Secondary accession number(s): D6VZB0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: March 19, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

Ribosomal proteins

Ribosomal proteins families and list of entries