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Protein

40S ribosomal protein S1-B

Gene

RPS1B

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • cytoplasmic translation Source: SGD
  • maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-32658-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_335938. Ribosomal scanning and start codon recognition.
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_351622. Formation of the ternary complex, and subsequently, the 43S complex.

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S1-BUniRule annotation
Alternative name(s):
RP10B
Gene namesi
Name:RPS1BUniRule annotation
Synonyms:PLC2, RPS10B
Ordered Locus Names:YML063W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML063W.
SGDiS000004528. RPS1B.

Subcellular locationi

GO - Cellular componenti

  • cytosolic small ribosomal subunit Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation1 Publication
Chaini2 – 25525440S ribosomal protein S1-BPRO_0000153542Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine; partialUniRule annotation
Modified residuei245 – 2451Phosphoserine3 Publications
Modified residuei254 – 2541Phosphothreonine2 Publications

Post-translational modificationi

N-terminally acetylated by acetyltransferase NatA.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP23248.
PeptideAtlasiP23248.

Interactioni

Subunit structurei

Component of the small ribosomal subunit. Mature ribosomes consist of a small (40S) and a large (60S) subunit. The 40S subunit contains 32 different proteins (encoded by 56 genes) and 1 molecule of RNA (18S). The 60S subunit contains 46 different proteins (encoded by 81 genes) and 3 molecules of RNA (25S, 5.8S and 5S).1 Publication

Protein-protein interaction databases

BioGridi35103. 159 interactions.
IntActiP23248. 74 interactions.
MINTiMINT-4496899.
STRINGi4932.YML063W.

Structurei

3D structure databases

ProteinModelPortaliP23248.
SMRiP23248. Positions 19-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribosomal protein S3Ae family.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00390000018433.
HOGENOMiHOG000105220.
InParanoidiP23248.
KOiK02984.
OMAiINQAASC.
OrthoDBiEOG7QVMDJ.

Family and domain databases

HAMAPiMF_03122. Ribosomal_S3Ae_euk.
InterProiIPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
[Graphical view]
PfamiPF01015. Ribosomal_S3Ae. 1 hit.
[Graphical view]
PROSITEiPS01191. RIBOSOMAL_S3AE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23248-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVGKNKRLS RGKKGLKKKV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK
60 70 80 90 100
STGLKNASDA LKGRVVEVCL ADLQGSEDHS FRKVKLRVDE VQGKNLLTNF
110 120 130 140 150
HGMDFTTDKL RSMVRKWQTL IEANVTVKTS DDYVLRIFAI AFTRKQANQV
160 170 180 190 200
KRHSYAQSSH IRAIRKVISE ILTREVQNST LAQLTSKLIP EVINKEIENA
210 220 230 240 250
TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK GKKVSGFKDE

VLETV
Length:255
Mass (Da):28,812
Last modified:January 23, 2007 - v3
Checksum:i192A913E3AD5C7DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55360 Genomic DNA. Translation: CAA39044.1.
Z38114 Genomic DNA. Translation: CAA86258.1.
AY557981 Genomic DNA. Translation: AAS56307.1.
BK006946 Genomic DNA. Translation: DAA09834.1.
PIRiS14051.
RefSeqiNP_013648.1. NM_001182422.1.

Genome annotation databases

EnsemblFungiiYML063W; YML063W; YML063W.
GeneIDi854939.
KEGGisce:YML063W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55360 Genomic DNA. Translation: CAA39044.1.
Z38114 Genomic DNA. Translation: CAA86258.1.
AY557981 Genomic DNA. Translation: AAS56307.1.
BK006946 Genomic DNA. Translation: DAA09834.1.
PIRiS14051.
RefSeqiNP_013648.1. NM_001182422.1.

3D structure databases

ProteinModelPortaliP23248.
SMRiP23248. Positions 19-233.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35103. 159 interactions.
IntActiP23248. 74 interactions.
MINTiMINT-4496899.
STRINGi4932.YML063W.

Proteomic databases

MaxQBiP23248.
PeptideAtlasiP23248.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYML063W; YML063W; YML063W.
GeneIDi854939.
KEGGisce:YML063W.

Organism-specific databases

EuPathDBiFungiDB:YML063W.
SGDiS000004528. RPS1B.

Phylogenomic databases

GeneTreeiENSGT00390000018433.
HOGENOMiHOG000105220.
InParanoidiP23248.
KOiK02984.
OMAiINQAASC.
OrthoDBiEOG7QVMDJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-32658-MONOMER.
ReactomeiREACT_290856. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_302067. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_307259. Formation of a pool of free 40S subunits.
REACT_314339. SRP-dependent cotranslational protein targeting to membrane.
REACT_332565. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_335938. Ribosomal scanning and start codon recognition.
REACT_343353. Peptide chain elongation.
REACT_346847. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_351622. Formation of the ternary complex, and subsequently, the 43S complex.

Miscellaneous databases

NextBioi977986.
PROiP23248.

Family and domain databases

HAMAPiMF_03122. Ribosomal_S3Ae_euk.
InterProiIPR027500. Ribosomal_S1/3_euk.
IPR001593. Ribosomal_S3Ae.
IPR018281. Ribosomal_S3Ae_CS.
[Graphical view]
PfamiPF01015. Ribosomal_S3Ae. 1 hit.
[Graphical view]
PROSITEiPS01191. RIBOSOMAL_S3AE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mitochondrial protein import: isolation and characterization of the Saccharomyces cerevisiae MFT1 gene."
    Garrett J.M., Singh K.K., Vonder Haar R.A., Emr S.D.
    Mol. Gen. Genet. 225:483-491(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: SEY2102.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "NH2-terminal acetylation of ribosomal proteins of Saccharomyces cerevisiae."
    Takakura H., Tsunasawa S., Miyagi M., Warner J.R.
    J. Biol. Chem. 267:5442-5445(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-21.
  6. "The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae."
    Planta R.J., Mager W.H.
    Yeast 14:471-477(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE, SUBUNIT.
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245 AND THR-254, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-245, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRS3A2_YEAST
AccessioniPrimary (citable) accession number: P23248
Secondary accession number(s): D6VZB0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 107000 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for S1 in yeast.

Caution

Was originally thought to be MFT1, the mitochondrial fusion target protein.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Ribosomal proteins
    Ribosomal proteins families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.