ID   PETD_CHLRE              Reviewed;         160 AA.
AC   P23230; B7U1E5;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   27-MAR-2024, entry version 145.
DE   RecName: Full=Cytochrome b6-f complex subunit 4 {ECO:0000255|HAMAP-Rule:MF_01344};
DE   AltName: Full=17 kDa polypeptide {ECO:0000255|HAMAP-Rule:MF_01344};
GN   Name=petD {ECO:0000255|HAMAP-Rule:MF_01344};
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=2137;
RX   PubMed=2017378; DOI=10.1093/nar/19.4.957;
RA   Yu W., Spreitzer R.J.;
RT   "Sequences of trnR-ACG and petD that contain a tRNA-like element within the
RT   chloroplast genome of Chlamydomonas reinhardtii.";
RL   Nucleic Acids Res. 19:957-957(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=137c / CC-125;
RX   PubMed=2060646; DOI=10.1016/0014-5793(91)80698-3;
RA   Bueschlen S., Choquet Y., Kuras R., Wollman F.A.;
RT   "Nucleotide sequences of the continuous and separated petA, petB and petD
RT   chloroplast genes in Chlamydomonas reinhardtii.";
RL   FEBS Lett. 284:257-262(1991).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CC-503;
RX   PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA   Smith D.R., Lee R.W.;
RT   "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT   addressing the mutational-hazard hypothesis.";
RL   BMC Evol. Biol. 9:120-120(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-8.
RC   STRAIN=CC-400;
RX   PubMed=8421681; DOI=10.1073/pnas.90.2.497;
RA   Sakamoto W., Kindle K.L., Stern D.B.;
RT   "In vivo analysis of Chlamydomonas chloroplast petD gene expression using
RT   stable transformation of beta-glucuronidase translational fusions.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:497-501(1993).
RN   [5]
RP   IDENTIFICATION, AND COMPLETE PLASTID GENOME.
RX   PubMed=12417694; DOI=10.1105/tpc.006155;
RA   Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA   Stern D.B.;
RT   "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT   sea of repeats.";
RL   Plant Cell 14:2659-2679(2002).
RN   [6]
RP   CHARACTERIZATION.
RC   STRAIN=WT12;
RX   PubMed=7493968; DOI=10.1074/jbc.270.49.29342;
RA   Pierre Y., Breyton C., Kramer D., Popot J.-L.;
RT   "Purification and characterization of the cytochrome b6 f complex from
RT   Chlamydomonas reinhardtii.";
RL   J. Biol. Chem. 270:29342-29349(1995).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS) OF 1-159.
RX   PubMed=14647374; DOI=10.1038/nature02155;
RA   Stroebel D., Choquet Y., Popot J.-L., Picot D.;
RT   "An atypical haem in the cytochrome b(6)f complex.";
RL   Nature 426:413-418(2003).
CC   -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC       electron transfer between photosystem II (PSII) and photosystem I
CC       (PSI), cyclic electron flow around PSI, and state transitions.
CC   -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC       cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and
CC       the Rieske protein, while the 4 small subunits are petG, petL, petM and
CC       petN. The complex functions as a dimer.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Multi-
CC       pass membrane protein.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- SIMILARITY: Belongs to the cytochrome b family. PetD subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_01344}.
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DR   EMBL; X56700; CAA40030.1; -; Genomic_DNA.
DR   EMBL; X72919; CAA51424.1; -; Genomic_DNA.
DR   EMBL; FJ423446; ACJ50092.1; -; Genomic_DNA.
DR   EMBL; L05506; AAA84153.1; -; Genomic_DNA.
DR   EMBL; BK000554; DAA00905.1; -; Genomic_DNA.
DR   PIR; S16918; S16918.
DR   RefSeq; NP_958359.1; NC_005353.1.
DR   PDB; 1Q90; X-ray; 3.10 A; D=1-159.
DR   PDBsum; 1Q90; -.
DR   AlphaFoldDB; P23230; -.
DR   SMR; P23230; -.
DR   IntAct; P23230; 1.
DR   STRING; 3055.P23230; -.
DR   PaxDb; 3055-DAA00905; -.
DR   GeneID; 2717021; -.
DR   KEGG; cre:ChreCp002; -.
DR   eggNOG; KOG4663; Eukaryota.
DR   HOGENOM; CLU_112652_0_0_1; -.
DR   InParanoid; P23230; -.
DR   BioCyc; CHLAMY:CHRECP002-MONOMER; -.
DR   BioCyc; MetaCyc:CHRECP002-MONOMER; -.
DR   EvolutionaryTrace; P23230; -.
DR   Proteomes; UP000006906; Chloroplast.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005750; C:mitochondrial respiratory chain complex III; IBA:GO_Central.
DR   GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0045156; F:electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity; IEA:InterPro.
DR   GO; GO:0006122; P:mitochondrial electron transport, ubiquinol to cytochrome c; IBA:GO_Central.
DR   GO; GO:0009767; P:photosynthetic electron transport chain; IEA:InterPro.
DR   GO; GO:1902600; P:proton transmembrane transport; IEA:GOC.
DR   CDD; cd00290; cytochrome_b_C; 1.
DR   Gene3D; 1.10.287.980; plastocyanin oxidoreductase; 1.
DR   Gene3D; 1.20.5.510; Single helix bin; 1.
DR   HAMAP; MF_01344; Cytb6_f_subIV; 1.
DR   InterPro; IPR005798; Cyt_b/b6_C.
DR   InterPro; IPR036150; Cyt_b/b6_C_sf.
DR   InterPro; IPR005870; Cyt_b6/f_cplx_suIV.
DR   InterPro; IPR048260; Cytochrome_b_C_euk/bac.
DR   NCBIfam; TIGR01156; cytb6_f_IV; 1.
DR   PANTHER; PTHR19271; CYTOCHROME B; 1.
DR   PANTHER; PTHR19271:SF16; CYTOCHROME B; 1.
DR   Pfam; PF00032; Cytochrom_B_C; 1.
DR   PIRSF; PIRSF000033; B6f_17K; 1.
DR   SUPFAM; SSF81648; a domain/subunit of cytochrome bc1 complex (Ubiquinol-cytochrome c reductase); 1.
DR   PROSITE; PS51003; CYTB_CTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chloroplast; Electron transport; Membrane; Photosynthesis;
KW   Plastid; Reference proteome; Thylakoid; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..160
FT                   /note="Cytochrome b6-f complex subunit 4"
FT                   /id="PRO_0000061852"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01344"
FT   TRANSMEM        95..115
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01344"
FT   TRANSMEM        131..151
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01344"
FT   CONFLICT        67
FT                   /note="N -> F (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   HELIX           12..20
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           24..26
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   STRAND          27..30
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           34..38
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           39..57
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           79..81
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           82..90
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           94..108
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   TURN            109..115
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           123..125
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           127..146
FT                   /evidence="ECO:0007829|PDB:1Q90"
FT   HELIX           151..154
FT                   /evidence="ECO:0007829|PDB:1Q90"
SQ   SEQUENCE   160 AA;  17442 MW;  6B1C77BBB8E857A8 CRC64;
     MSVTKKPDLS DPVLKAKLAK GMGHNTYGEP AWPNDLLYMF PVVILGTFAC VIGLSVLDPA
     AMGEPANPFA TPLEILPEWY FYPVFQILRV VPNKLLGVLL MAAVPAGLIT VPFIESINKF
     QNPYRRPIAT ILFLLGTLVA VWLGIGSTFP IDISLTLGLF
//