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Protein

Hydroxymethylglutaryl-CoA synthase, cytoplasmic

Gene

HMGCS1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.

Catalytic activityi

Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.PROSITE-ProRule annotation

Pathway:i(R)-mevalonate biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes (R)-mevalonate from acetyl-CoA.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Hydroxymethylglutaryl-CoA synthase, cytoplasmic (HMGCS1)
  3. 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR), 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR)
This subpathway is part of the pathway (R)-mevalonate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-mevalonate from acetyl-CoA, the pathway (R)-mevalonate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Proton donor/acceptorPROSITE-ProRule annotation
Active sitei129 – 1291Acyl-thioester intermediatePROSITE-ProRule annotation
Active sitei264 – 2641Proton donor/acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Enzyme and pathway databases

BRENDAi2.3.3.10. 1306.
SABIO-RKP23228.
UniPathwayiUPA00058; UER00102.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic (EC:2.3.3.10)
Short name:
HMG-CoA synthase
Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase
Gene namesi
Name:HMGCS1
Synonyms:HMGCS
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Hydroxymethylglutaryl-CoA synthase, cytoplasmicPRO_0000213751Add
BLAST

Proteomic databases

PaxDbiP23228.
PRIDEiP23228.

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038170.

Structurei

3D structure databases

ProteinModelPortaliP23228.
SMRiP23228. Positions 16-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMG-CoA synthase family.Curated

Phylogenomic databases

eggNOGiCOG3425.
HOGENOMiHOG000012351.
HOVERGENiHBG051912.
InParanoidiP23228.
KOiK01641.
PhylomeDBiP23228.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23228-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGSLPVNTE SCWPKDVGIV ALEIYFPSQY VDQTELEKYD GVDAGKYTIG
60 70 80 90 100
LGQSKMGFCS DREDINSLCL TVVQKLMERN SLSYDCIGRL EVGTETIIDK
110 120 130 140 150
SKSVKTVLMQ LFEESGNTDV EGIDTTNACY GGTAALFNAI NWIESSSWDG
160 170 180 190 200
RYALVVAGDI AVYATGNARP TGGAGAVAML VGSNAPLIFE RGLRGTHMQH
210 220 230 240 250
AYDFYKPDMV SEYPVVDGKL SIQCYLSALD RCYSVYRNKI HAQWQKEGTD
260 270 280 290 300
RGFTLNDFGF MIFHSPYCKL VQKSVARLLL NDFLSDQNAE TANGVFSGLE
310 320 330 340 350
AFRDVKLEDT YFDRDVEKAF MKASAELFNQ KTKASLLVSN QNGNMYTPSV
360 370 380 390 400
YGCLASLLAQ YSPEHLAGQR ISEFSYGSGF AATLYSIRVT QDATPGSALD
410 420 430 440 450
KITASLSDLK ARLDSRKCIA PDVFAENMKI RQETHHLANY IPQCSVEDLF
460 470 480 490 500
EGTWYLVRVD EKHRRTYARR PVMGDGPLEA GVEVVHPGIV HEHIPSPAKK
510 520
VPRIPATTES EGVTVAISNG VH
Length:522
Mass (Da):57,559
Last modified:November 1, 1991 - v1
Checksum:iBFF7947C3E963C4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60657 mRNA. Translation: AAA62737.1.
PIRiS13887.
RefSeqiNP_990742.1. NM_205411.1.
UniGeneiGga.4388.

Genome annotation databases

GeneIDi396379.
KEGGigga:396379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60657 mRNA. Translation: AAA62737.1.
PIRiS13887.
RefSeqiNP_990742.1. NM_205411.1.
UniGeneiGga.4388.

3D structure databases

ProteinModelPortaliP23228.
SMRiP23228. Positions 16-471.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000038170.

Proteomic databases

PaxDbiP23228.
PRIDEiP23228.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396379.
KEGGigga:396379.

Organism-specific databases

CTDi3157.

Phylogenomic databases

eggNOGiCOG3425.
HOGENOMiHOG000012351.
HOVERGENiHBG051912.
InParanoidiP23228.
KOiK01641.
PhylomeDBiP23228.

Enzyme and pathway databases

UniPathwayiUPA00058; UER00102.
BRENDAi2.3.3.10. 1306.
SABIO-RKP23228.

Miscellaneous databases

NextBioi20816421.
PROiP23228.

Family and domain databases

Gene3Di3.40.47.10. 1 hit.
InterProiIPR000590. HMG_CoA_synt_AS.
IPR013746. HMG_CoA_synt_C_dom.
IPR013528. HMG_CoA_synth_N.
IPR010122. HMG_CoA_synthase_euk.
IPR016039. Thiolase-like.
[Graphical view]
PfamiPF08540. HMG_CoA_synt_C. 1 hit.
PF01154. HMG_CoA_synt_N. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 3 hits.
TIGRFAMsiTIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITEiPS01226. HMG_COA_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Avian liver 3-hydroxy-3-methylglutaryl-CoA synthase: distinct genes encode the cholesterogenic and ketogenic isozymes."
    Kattar-Cooley P.A., Wang H.-H.L., Mende-Mueller L.M., Miziorko H.M.
    Arch. Biochem. Biophys. 283:523-529(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Avian cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase: evaluation of the role of cysteines in reaction chemistry."
    Misra I., Charlier H.A. Jr., Miziorko H.M.
    Biochim. Biophys. Acta 1247:253-259(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, MUTAGENESIS.

Entry informationi

Entry nameiHMCS1_CHICK
AccessioniPrimary (citable) accession number: P23228
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 22, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.