P23228 (HMCS1_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic
Short name=HMG-CoA synthase
EC=2.3.3.10

Alternative name(s):
3-hydroxy-3-methylglutaryl coenzyme A synthase

Gene names

Name:	HMGCS1
Synonyms:	HMGCS

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Catalytic activity	Acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the HMG-CoA synthase family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Cholesterol metabolism Lipid biosynthesis Lipid metabolism Steroid biosynthesis Steroid metabolism Sterol biosynthesis Sterol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW isoprenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	hydroxymethylglutaryl-CoA synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Hydroxymethylglutaryl-CoA synthase, cytoplasmic		PRO_0000213751
Sites
	Active site	129	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	P23228 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: BFF7947C3E963C4C	FASTA	522	57,559
	10 20 30 40 50 60 MPGSLPVNTE SCWPKDVGIV ALEIYFPSQY VDQTELEKYD GVDAGKYTIG LGQSKMGFCS 70 80 90 100 110 120 DREDINSLCL TVVQKLMERN SLSYDCIGRL EVGTETIIDK SKSVKTVLMQ LFEESGNTDV 130 140 150 160 170 180 EGIDTTNACY GGTAALFNAI NWIESSSWDG RYALVVAGDI AVYATGNARP TGGAGAVAML 190 200 210 220 230 240 VGSNAPLIFE RGLRGTHMQH AYDFYKPDMV SEYPVVDGKL SIQCYLSALD RCYSVYRNKI 250 260 270 280 290 300 HAQWQKEGTD RGFTLNDFGF MIFHSPYCKL VQKSVARLLL NDFLSDQNAE TANGVFSGLE 310 320 330 340 350 360 AFRDVKLEDT YFDRDVEKAF MKASAELFNQ KTKASLLVSN QNGNMYTPSV YGCLASLLAQ 370 380 390 400 410 420 YSPEHLAGQR ISEFSYGSGF AATLYSIRVT QDATPGSALD KITASLSDLK ARLDSRKCIA 430 440 450 460 470 480 PDVFAENMKI RQETHHLANY IPQCSVEDLF EGTWYLVRVD EKHRRTYARR PVMGDGPLEA 490 500 510 520 GVEVVHPGIV HEHIPSPAKK VPRIPATTES EGVTVAISNG VH

References

[1]	"Avian liver 3-hydroxy-3-methylglutaryl-CoA synthase: distinct genes encode the cholesterogenic and ketogenic isozymes." Kattar-Cooley P.A., Wang H.-H.L., Mende-Mueller L.M., Miziorko H.M. Arch. Biochem. Biophys. 283:523-529(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Avian cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase: evaluation of the role of cysteines in reaction chemistry." Misra I., Charlier H.A. Jr., Miziorko H.M. Biochim. Biophys. Acta 1247:253-259(1995) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION, MUTAGENESIS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60657 mRNA. Translation: AAA62737.1.
IPI	IPI00577321.
PIR	S13887.
RefSeq	NP_990742.1. NM_205411.1.
UniGene	Gga.4388.
3D structure databases
ProteinModelPortal	P23228.
SMR	P23228. Positions 16-471.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000038170.
Proteomic databases
PaxDb	P23228.
PRIDE	P23228.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396379.
KEGG	gga:396379.
Organism-specific databases
CTD	3157.
Phylogenomic databases
eggNOG	COG3425.
HOGENOM	HOG000012351.
HOVERGEN	HBG051912.
InParanoid	P23228.
KO	K01641.
OrthoDB	EOG4F1X30.
Enzyme and pathway databases
BRENDA	2.3.3.10. 1306.
SABIO-RK	P23228.
UniPathway	UPA00058; UER00102.
Family and domain databases
Gene3D	3.40.47.10. 1 hit.
InterPro	IPR000590. HMG_CoA_synt_AS. IPR013746. HMG_CoA_synt_C. IPR013528. HMG_CoA_synth_N. IPR010122. HMG_CoA_synthase_euk. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Pfam	PF08540. HMG_CoA_synt_C. 1 hit. PF01154. HMG_CoA_synt_N. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01833. HMG-CoA-S_euk. 1 hit.
PROSITE	PS01226. HMG_COA_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816421.

Entry information

Entry name

HMCS1_CHICK

Accession

Primary (citable) accession number: P23228

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program