Reviewed, UniProtKB/Swiss-Prot P23225 (GLTB_MAIZE)

Names and origin

Protein names

Recommended name:
    Ferredoxin-dependent glutamate synthase, chloroplastic
    EC=1.4.7.1
Alternative name(s):
    Fd-GOGAT

Gene names

Name:

GLSF

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	1616 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.
Cofactor	Binds 1 3Fe-4S cluster. FAD. FMN.
Pathway	Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1. Energy metabolism; nitrogen metabolism.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast stroma.
Tissue specificity	Mostly in green tissues and lesser in non-photosynthetic tissues.
Sequence similarities	Belongs to the glutamate synthase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Glutamate biosynthesis
Cellular component	Chloroplast Plastid
Domain	Glutamine amidotransferase Transit peptide
Ligand	3Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate synthase (ferredoxin) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 97	97	Chloroplast
	Chain	98 – 1616	1519	Ferredoxin-dependent glutamate synthase, chloroplastic		PRO_0000011616
Regions
	Domain	98 – 497	400	Glutamine amidotransferase type-2
	Nucleotide binding	1176 – 1233	58	FMN By similarity
Sites
	Active site	98	1	For GATase activity By similarity
	Metal binding	1229	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1235	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1240	1	Iron-sulfur (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23225-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 67724CBFA0AEB053	FASTA	1,616	175,173
	10 20 30 40 50 60 MATLPRAAPP TPAALLPLPR AAPPLLLAGR AAAARRSRLR ARGPSAAARR SWVVASAASS 70 80 90 100 110 120 SSRAVVGGVA RREAPPAPQK PTQQAADLNH ILSERGACGV GFVANLKNMS SFDIVRDALM 130 140 150 160 170 180 ALGCMEHRGG CGADSDSGDG AGLMSAVPWD LFDDWASKQG LALFDRRNTG VGMVFLPQDE 190 200 210 220 230 240 KSMEEAKAAT EKVFVDEGLE VLGWRPVPFN VSVVGRNAKE TMPNIQQIFV KVAKEDNADD 250 260 270 280 290 300 IERELYISRK LIERAAKSFS WADELYFCSL SSRTIVYKGM LRSEVLGQFY LDLQNELYKS 310 320 330 340 350 360 PFAIYHRRFS TNTSPRWPLA QPMRLLGHNG EINTIQGNLN WMRSRETTLK SPVWRGREHE 370 380 390 400 410 420 ICPFGDPKAS DSANLDSTAE LLLRSGRSPA EALMILVPEA YKNHPTLSIK YPEVTDFYDY 430 440 450 460 470 480 YKGQMEAWDG PALLLFSDGR TVGATLDRNG LRPARYWRTS DDFVYVASEV GVIPMDESKV 490 500 510 520 530 540 VMKGRLGPGM MITVDLQTGQ VLENTEVKKT VASASPYGTW LQECTRLIKP VNFLSSTIMD 550 560 570 580 590 600 NETVLRHQQA FGYSSEDVQM VIESMASQGK EPTFCMGDDI PLAVLSQRPH LLYDYFKQRF 610 620 630 640 650 660 AQVTNPAIDP LREGLVMSLE VNIGKRGNIL EVGPENADQV ALSSPVLNEG ELETLLNDSK 670 680 690 700 710 720 LKPKVLSTYF DIRKGLDGSL DKTIQALCEE ADAAVRSGSQ LLVLSDRSEA PEPTRPAIPI 730 740 750 760 770 780 LLAVGAIHQH LIQNGLRMSA SIVADTAQCF STHHFACLIG YGASAVCPYL ALETCRQWRL 790 800 810 820 830 840 SNKTLNLMRN GKMPTVTIEQ AQRNFIKAVK SGLLKILSKM GISLLSSYCG AQIFEIYGLG 850 860 870 880 890 900 QEVVDLAFCG SVSKIGGLTL DELGRETLSF WVKAFSEDTA KRLENFGFIQ SRPGGEYHAN 910 920 930 940 950 960 NPEMSKLLHK AIREKRDNAY TVYQQHLASR PVNVLRDLLE LKSDRAPIPI GKVESATSIV 970 980 990 1000 1010 1020 ERFCTGGMSL GAISRETHEA IAIAMNRIGG KSNSGEGGED PIRWNPLTDV VDGYSPTLPH 1030 1040 1050 1060 1070 1080 LKGLQNGDTA TSAIKQVASG RFGVTPTFLV NADQIEIKIA QGAKPGEGGQ LPGKKVSAYI 1090 1100 1110 1120 1130 1140 ARLRNSKPGV PLISPPPHHD IYSIEDLAQL IYDLHQINPK AKVSVKLVSE AGIGTVASGV 1150 1160 1170 1180 1190 1200 SKANADIIQI SGHDGGTGAS PISSIKHAGG PWELGLTETN QTLIQNGLRE RVVLRVDGGF 1210 1220 1230 1240 1250 1260 RSGQDVLIAA AMGADEYGFG SVAMIATGCV MARICHTNNC PVGVASQREE LRARFPGVPG 1270 1280 1290 1300 1310 1320 DLVNYFLFVA EEVRAALAQL GYEKLDDIIG RTDLLKPKHI SLVKTQHIDL GYLLSNAGLP 1330 1340 1350 1360 1370 1380 EWSSSQIRSQ DVHTNGPVLD ETILADPEIA DAIENEKEVS KAFQIYNVDR AVCGRVAGVI 1390 1400 1410 1420 1430 1440 AKKYGDTGFA GQLNITFNGS AGQSFGCFLT PGMNIRLVGE ANDYVGKGMA GGELVVVPVD 1450 1460 1470 1480 1490 1500 KTGFVPEDAT IVGNTCLYGA TGGQVFVRGK AGERFAVRNS LCQAVVEGTG DHCCEYMTGG 1510 1520 1530 1540 1550 1560 CVVVLGKAGR NVAAGMTGGL AYILDEDDTL VPKVNKEIVK MQRVNAPAGQ MQLKGLIEAY 1570 1580 1590 1600 1610 VEKTGSEKGI AILREWEAYL PLFWQLVPPS EEDSPEACAE FERVLAKQAT TQLSAK

References

[1]

"Molecular cloning and characterization of complementary DNA encoding for ferredoxin-dependent glutamate synthase in maize leaf." Sakakibara H., Watanabe M., Hase T., Sugiyama T. J. Biol. Chem. 266:2028-2035(1991) [PubMed: 1989968] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 97-109 AND 790-802. Strain: cv. Golden cross Bantam T51. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M59190 mRNA. Translation: AAA33463.1.
PIR	A38596.
RefSeq	NP_001105693.1.
UniGene	Zm.54067
3D structure databases
HSSP	HSSP built from PDB template 1OFD based on UniProtKB P55038.
ModBase	Search...
Genome annotation databases
GeneID	542710.
Organism-specific databases
Gramene	P23225.
MaizeGDB	40403.
Enzyme and pathway databases
BRENDA	1.4.7.1. 289.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000583. GATase_2. IPR017932. GATase_II. IPR002932. Glu_synth_centr_C. IPR006982. Glu_synth_centr_N. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits. G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF04898. Glu_syn_central. 1 hit. PF01645. Glu_synthase. 1 hit. PF01493. GXGXG. 1 hit. [Graphical view]
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTB_MAIZE

Accession

Primary (citable) accession number: P23225

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)