P23222 (FIXL_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sensor protein fixL
EC=2.7.13.3

Gene names

Name:	fixL
Ordered Locus Names:	bll2760

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ.
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Cofactor	Binds 1 heme group per subunit.
Enzyme regulation	The heme moiety regulates the kinase activity.
Sequence similarities	Contains 1 histidine kinase domain. Contains 2 PAC (PAS-associated C-terminal) domains. Contains 2 PAS (PER-ARNT-SIM) domains.

Ontologies

Keywords
Biological process	Nitrogen fixation Two-component regulatory system
Domain	Repeat
Ligand	ATP-binding Heme Iron Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-histidine phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW two-component sensor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 505

505

Sensor protein fixL

PRO_0000074764

Regions

Domain

14 – 85

72

PAS 1

Domain

88 – 140

53

PAC 1

Domain

141 – 208

68

PAS 2

Domain

209 – 268

60

PAC 2

Domain

288 – 503

216

Histidine kinase

Sites

Metal binding

200

1

Iron (heme axial ligand)

Amino acid modifications

Modified residue

291

1

Phosphohistidine; by autocatalysis By similarity

Secondary structure

1

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505

Helix Strand Turn

Details...

Beta strand

155 – 160

6

Beta strand

163 – 168

6

Helix

170 – 176

7

Helix

180 – 183

4

Helix

188 – 191

4

Helix

196 – 210

15

Beta strand

214 – 216

3

Beta strand

219 – 225

7

Beta strand

231 – 243

13

Beta strand

246 – 255

10

Helix

257 – 268

12

Sequences

	Sequence		Length	Mass (Da)
	P23222 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 1854A0C7EDAC32C6	FASTA	505	55,652
	10 20 30 40 50 60 MAPTRVTHPP DDGRGEHFRV RIEGFGVGTW DLDLKTWALD WSDTARTLLG IGQDQPASYD 70 80 90 100 110 120 LFLSRLEPDD RERVESAIKR VSERGGGFDV SFRVAGTSNA GQWIRARAGL IRDEAGTARH 130 140 150 160 170 180 LSGIFLDIDE EKQVEGALRT RETHLRSILH TIPDAMIVID GHGIIQLFST AAERLFGWSE 190 200 210 220 230 240 LEAIGQNVNI LMPEPDRSRH DSYISRYRTT SDPHIIGIGR IVTGKRRDGT TFPMHLSIGE 250 260 270 280 290 300 MQSGGEPYFT GFVRDLTEHQ QTQARLQELQ SELVHVSRLS AMGEMASALA HELNQPLAAI 310 320 330 340 350 360 SNYMKGSRRL LAGSSDPNTP KVESALDRAA EQALRAGQII RRLRDFVARG ESEKRVESLS 370 380 390 400 410 420 KLIEEAGALG LAGAREQNVQ LRFSLDPGAD LVLADRVQIQ QVLVNLFRNA LEAMAQSQRR 430 440 450 460 470 480 ELVVTNTPAA DDMIEVEVSD TGSGFQDDVI PNLFQTFFTT KDTGMGVGLS ISRSIIEAHG 490 500 GRMWAESNAS GGATFRFTLP AADEN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The regulatory status of the fixL- and fixJ-like genes in Bradyrhizobium japonicum may be different from that in Rhizobium meliloti." Anthamatten D., Hennecke H. Mol. Gen. Genet. 225:38-48(1991) [PubMed: 2000090] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: USDA 110spc4.
[2]	"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.
[3]	"Structure of a biological oxygen sensor: a new mechanism for heme-driven signal transduction." Gong W., Hao B., Mansy S.S., Gonzalez G., Gilles-Gonzalez M.-A., Chan M.K. Proc. Natl. Acad. Sci. U.S.A. 95:15177-15182(1998) [PubMed: 9860942] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 154-270.
[4]	"New mechanistic insights from structural studies of the oxygen-sensing domain of Bradyrhizobium japonicum FixL." Gong W., Hao B., Chan M.K. Biochemistry 39:3955-3962(2000) [PubMed: 10747783] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 154-270.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X56808 Genomic DNA. Translation: CAA40143.1.
AJ005001 Genomic DNA. Translation: CAA06276.1.
BA000040 Genomic DNA. Translation: BAC48025.1.

PIR

S13330.

RefSeq

NP_769400.1. NC_004463.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DP6	X-ray	2.30	A	141-270	[»]
1DP8	X-ray	2.50	A	141-270	[»]
1DP9	X-ray	2.60	A	141-270	[»]
1DRM	X-ray	2.40	A	141-270	[»]
1LSV	X-ray	2.40	A	141-270	[»]
1LSW	X-ray	2.20	A	141-270	[»]
1LSX	X-ray	2.70	A	141-270	[»]
1LT0	X-ray	2.40	A	141-270	[»]
1XJ2	X-ray	2.00	A	154-269	[»]
1XJ3	X-ray	1.90	A	154-269	[»]
1XJ4	X-ray	1.80	A/B	151-269	[»]
1XJ6	X-ray	1.90	A/B	151-269	[»]
1Y28	X-ray	2.10	A	141-270	[»]
2CMN	X-ray	2.30	A	141-270	[»]
2OWH	X-ray	2.50	A	154-269	[»]
2OWJ	X-ray	2.50	A	154-269	[»]
2VV6	X-ray	1.50	A/B/C/D	151-269	[»]
2VV7	X-ray	1.81	A/B/C/D	151-269	[»]
2VV8	X-ray	1.61	A/B/C/D	151-269	[»]

ProteinModelPortal

P23222.

SMR

P23222. Positions 152-270.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1054108.

GenomeReviews

Gene locus bll2760 in contig BA000040_GR.

KEGG

bja:bll2760.

NMPDR

fig|224911.1.peg.2760.

PATRIC

21188968. VBIBraJap65052_2742.

Phylogenomic databases

HOGENOM

HBG334875.

OMA

LRTRETH.

PhylomeDB

P23222.

ProtClustDB

CLSK910877.

Enzyme and pathway databases

BioCyc

BJAP224911:BLL2760-MONOMER.

BRENDA

2.7.13.3. 929.

Family and domain databases

InterPro

IPR003594. ATPase-like_ATP-bd.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view]

Gene3D

G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit.

KO

K14986.

Pfam

PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view]

PRINTS

PR00344. BCTRLSENSOR.

SMART

SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]

SUPFAM

SSF55874. ATP_bd_ATPase. 1 hit.
SSF47384. His_kin_homodim. 1 hit.

TIGRFAMs

TIGR00229. Sensory_box. 2 hits.

PROSITE

PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 2 hits.
PS50112. PAS. 2 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FIXL_BRAJA

Accession

Primary (citable) accession number: P23222

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program