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Protein

Sensor protein FixL

Gene

fixL

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

hemeNote: Binds 1 heme group per subunit.

Enzyme regulationi

The heme moiety regulates the kinase activity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi200 – 2001Iron (heme axial ligand)

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nitrogen fixation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Heme, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-2784-MONOMER.
BRENDAi2.7.13.3. 929.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein FixL (EC:2.7.13.3)
Gene namesi
Name:fixL
Ordered Locus Names:bll2760
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Sensor protein FixLPRO_0000074764Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei291 – 2911Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi224911.bll2760.

Structurei

Secondary structure

1
505
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi155 – 1606Combined sources
Beta strandi163 – 1686Combined sources
Helixi170 – 1767Combined sources
Helixi180 – 1834Combined sources
Helixi188 – 1914Combined sources
Helixi196 – 21015Combined sources
Turni216 – 2183Combined sources
Beta strandi220 – 2256Combined sources
Beta strandi231 – 24313Combined sources
Beta strandi246 – 25510Combined sources
Helixi257 – 26812Combined sources
Helixi277 – 31135Combined sources
Helixi319 – 34729Combined sources
Beta strandi355 – 3584Combined sources
Helixi359 – 37012Combined sources
Turni371 – 3788Combined sources
Beta strandi380 – 3845Combined sources
Helixi387 – 3893Combined sources
Beta strandi391 – 3944Combined sources
Helixi396 – 41318Combined sources
Turni414 – 4163Combined sources
Beta strandi421 – 4299Combined sources
Turni430 – 4323Combined sources
Beta strandi433 – 4397Combined sources
Turni447 – 4493Combined sources
Helixi450 – 4523Combined sources
Helixi468 – 47811Combined sources
Beta strandi483 – 4875Combined sources
Beta strandi491 – 50111Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DP6X-ray2.30A141-270[»]
1DP8X-ray2.50A141-270[»]
1DP9X-ray2.60A141-270[»]
1DRMX-ray2.40A141-270[»]
1LSVX-ray2.40A141-270[»]
1LSWX-ray2.20A141-270[»]
1LSXX-ray2.70A141-270[»]
1LT0X-ray2.40A141-270[»]
1XJ2X-ray2.00A154-269[»]
1XJ3X-ray1.90A154-269[»]
1XJ4X-ray1.80A/B151-269[»]
1XJ6X-ray1.90A/B151-269[»]
1Y28X-ray2.10A141-270[»]
2CMNX-ray2.30A141-270[»]
2OWHX-ray2.50A154-269[»]
2OWJX-ray2.50A154-269[»]
2VV6X-ray1.50A/B/C/D151-269[»]
2VV7X-ray1.81A/B/C/D151-269[»]
2VV8X-ray1.61A/B/C/D151-269[»]
4GCZX-ray2.30A/B257-505[»]
ProteinModelPortaliP23222.
SMRiP23222. Positions 152-270.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23222.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 8572PAS 1PROSITE-ProRule annotationAdd
BLAST
Domaini88 – 14053PAC 1PROSITE-ProRule annotationAdd
BLAST
Domaini141 – 20868PAS 2PROSITE-ProRule annotationAdd
BLAST
Domaini209 – 26860PAC 2PROSITE-ProRule annotationAdd
BLAST
Domaini288 – 503216Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 2 PAC (PAS-associated C-terminal) domains.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000252047.
InParanoidiP23222.
KOiK14986.
OMAiHARILIF.
OrthoDBiPOG091H0L6P.
PhylomeDBiP23222.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 2 hits.
PS50112. PAS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPTRVTHPP DDGRGEHFRV RIEGFGVGTW DLDLKTWALD WSDTARTLLG
60 70 80 90 100
IGQDQPASYD LFLSRLEPDD RERVESAIKR VSERGGGFDV SFRVAGTSNA
110 120 130 140 150
GQWIRARAGL IRDEAGTARH LSGIFLDIDE EKQVEGALRT RETHLRSILH
160 170 180 190 200
TIPDAMIVID GHGIIQLFST AAERLFGWSE LEAIGQNVNI LMPEPDRSRH
210 220 230 240 250
DSYISRYRTT SDPHIIGIGR IVTGKRRDGT TFPMHLSIGE MQSGGEPYFT
260 270 280 290 300
GFVRDLTEHQ QTQARLQELQ SELVHVSRLS AMGEMASALA HELNQPLAAI
310 320 330 340 350
SNYMKGSRRL LAGSSDPNTP KVESALDRAA EQALRAGQII RRLRDFVARG
360 370 380 390 400
ESEKRVESLS KLIEEAGALG LAGAREQNVQ LRFSLDPGAD LVLADRVQIQ
410 420 430 440 450
QVLVNLFRNA LEAMAQSQRR ELVVTNTPAA DDMIEVEVSD TGSGFQDDVI
460 470 480 490 500
PNLFQTFFTT KDTGMGVGLS ISRSIIEAHG GRMWAESNAS GGATFRFTLP

AADEN
Length:505
Mass (Da):55,652
Last modified:November 1, 1991 - v1
Checksum:i1854A0C7EDAC32C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56808 Genomic DNA. Translation: CAA40143.1.
AJ005001 Genomic DNA. Translation: CAA06276.1.
BA000040 Genomic DNA. Translation: BAC48025.1.
PIRiS13330.
RefSeqiNP_769400.1. NC_004463.1.
WP_011085545.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC48025; BAC48025; BAC48025.
GeneIDi1054108.
KEGGibja:bll2760.
PATRICi21188968. VBIBraJap65052_2742.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56808 Genomic DNA. Translation: CAA40143.1.
AJ005001 Genomic DNA. Translation: CAA06276.1.
BA000040 Genomic DNA. Translation: BAC48025.1.
PIRiS13330.
RefSeqiNP_769400.1. NC_004463.1.
WP_011085545.1. NC_004463.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1DP6X-ray2.30A141-270[»]
1DP8X-ray2.50A141-270[»]
1DP9X-ray2.60A141-270[»]
1DRMX-ray2.40A141-270[»]
1LSVX-ray2.40A141-270[»]
1LSWX-ray2.20A141-270[»]
1LSXX-ray2.70A141-270[»]
1LT0X-ray2.40A141-270[»]
1XJ2X-ray2.00A154-269[»]
1XJ3X-ray1.90A154-269[»]
1XJ4X-ray1.80A/B151-269[»]
1XJ6X-ray1.90A/B151-269[»]
1Y28X-ray2.10A141-270[»]
2CMNX-ray2.30A141-270[»]
2OWHX-ray2.50A154-269[»]
2OWJX-ray2.50A154-269[»]
2VV6X-ray1.50A/B/C/D151-269[»]
2VV7X-ray1.81A/B/C/D151-269[»]
2VV8X-ray1.61A/B/C/D151-269[»]
4GCZX-ray2.30A/B257-505[»]
ProteinModelPortaliP23222.
SMRiP23222. Positions 152-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.bll2760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC48025; BAC48025; BAC48025.
GeneIDi1054108.
KEGGibja:bll2760.
PATRICi21188968. VBIBraJap65052_2742.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000252047.
InParanoidiP23222.
KOiK14986.
OMAiHARILIF.
OrthoDBiPOG091H0L6P.
PhylomeDBiP23222.

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-2784-MONOMER.
BRENDAi2.7.13.3. 929.

Miscellaneous databases

EvolutionaryTraceiP23222.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 2 hits.
PS50112. PAS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIXL_BRADU
AccessioniPrimary (citable) accession number: P23222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.