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Protein

Sensor protein FixL

Gene

fixL

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Putative oxygen sensor; modulates the activity of FixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates FixJ.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

hemeNote: Binds 1 heme group per subunit.

Enzyme regulationi

The heme moiety regulates the kinase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi200Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nitrogen fixation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Heme, Iron, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.13.3. 929.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein FixL (EC:2.7.13.3)
Gene namesi
Name:fixL
Ordered Locus Names:bll2760
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000747641 – 505Sensor protein FixLAdd BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei291Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi224911.bll2760.

Structurei

Secondary structure

1505
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi155 – 160Combined sources6
Beta strandi163 – 168Combined sources6
Helixi170 – 176Combined sources7
Helixi180 – 183Combined sources4
Helixi188 – 191Combined sources4
Helixi196 – 210Combined sources15
Turni216 – 218Combined sources3
Beta strandi220 – 225Combined sources6
Beta strandi231 – 243Combined sources13
Beta strandi246 – 255Combined sources10
Helixi257 – 268Combined sources12
Helixi277 – 311Combined sources35
Helixi319 – 347Combined sources29
Beta strandi355 – 358Combined sources4
Helixi359 – 370Combined sources12
Turni371 – 378Combined sources8
Beta strandi380 – 384Combined sources5
Helixi387 – 389Combined sources3
Beta strandi391 – 394Combined sources4
Helixi396 – 413Combined sources18
Turni414 – 416Combined sources3
Beta strandi421 – 429Combined sources9
Turni430 – 432Combined sources3
Beta strandi433 – 439Combined sources7
Turni447 – 449Combined sources3
Helixi450 – 452Combined sources3
Helixi468 – 478Combined sources11
Beta strandi483 – 487Combined sources5
Beta strandi491 – 501Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DP6X-ray2.30A141-270[»]
1DP8X-ray2.50A141-270[»]
1DP9X-ray2.60A141-270[»]
1DRMX-ray2.40A141-270[»]
1LSVX-ray2.40A141-270[»]
1LSWX-ray2.20A141-270[»]
1LSXX-ray2.70A141-270[»]
1LT0X-ray2.40A141-270[»]
1XJ2X-ray2.00A154-269[»]
1XJ3X-ray1.90A154-269[»]
1XJ4X-ray1.80A/B151-269[»]
1XJ6X-ray1.90A/B151-269[»]
1Y28X-ray2.10A141-270[»]
2CMNX-ray2.30A141-270[»]
2OWHX-ray2.50A154-269[»]
2OWJX-ray2.50A154-269[»]
2VV6X-ray1.50A/B/C/D151-269[»]
2VV7X-ray1.81A/B/C/D151-269[»]
2VV8X-ray1.61A/B/C/D151-269[»]
4GCZX-ray2.30A/B257-505[»]
ProteinModelPortaliP23222.
SMRiP23222.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23222.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 85PAS 1PROSITE-ProRule annotationAdd BLAST72
Domaini88 – 140PAC 1PROSITE-ProRule annotationAdd BLAST53
Domaini141 – 208PAS 2PROSITE-ProRule annotationAdd BLAST68
Domaini209 – 268PAC 2PROSITE-ProRule annotationAdd BLAST60
Domaini288 – 503Histidine kinasePROSITE-ProRule annotationAdd BLAST216

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 2 PAC (PAS-associated C-terminal) domains.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000252047.
InParanoidiP23222.
KOiK14986.
OMAiHARILIF.
OrthoDBiPOG091H0L6P.
PhylomeDBiP23222.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 2 hits.
PS50112. PAS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPTRVTHPP DDGRGEHFRV RIEGFGVGTW DLDLKTWALD WSDTARTLLG
60 70 80 90 100
IGQDQPASYD LFLSRLEPDD RERVESAIKR VSERGGGFDV SFRVAGTSNA
110 120 130 140 150
GQWIRARAGL IRDEAGTARH LSGIFLDIDE EKQVEGALRT RETHLRSILH
160 170 180 190 200
TIPDAMIVID GHGIIQLFST AAERLFGWSE LEAIGQNVNI LMPEPDRSRH
210 220 230 240 250
DSYISRYRTT SDPHIIGIGR IVTGKRRDGT TFPMHLSIGE MQSGGEPYFT
260 270 280 290 300
GFVRDLTEHQ QTQARLQELQ SELVHVSRLS AMGEMASALA HELNQPLAAI
310 320 330 340 350
SNYMKGSRRL LAGSSDPNTP KVESALDRAA EQALRAGQII RRLRDFVARG
360 370 380 390 400
ESEKRVESLS KLIEEAGALG LAGAREQNVQ LRFSLDPGAD LVLADRVQIQ
410 420 430 440 450
QVLVNLFRNA LEAMAQSQRR ELVVTNTPAA DDMIEVEVSD TGSGFQDDVI
460 470 480 490 500
PNLFQTFFTT KDTGMGVGLS ISRSIIEAHG GRMWAESNAS GGATFRFTLP

AADEN
Length:505
Mass (Da):55,652
Last modified:November 1, 1991 - v1
Checksum:i1854A0C7EDAC32C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56808 Genomic DNA. Translation: CAA40143.1.
AJ005001 Genomic DNA. Translation: CAA06276.1.
BA000040 Genomic DNA. Translation: BAC48025.1.
PIRiS13330.
RefSeqiNP_769400.1. NC_004463.1.
WP_011085545.1. NZ_CP011360.1.

Genome annotation databases

EnsemblBacteriaiBAC48025; BAC48025; BAC48025.
GeneIDi1054108.
KEGGibja:bll2760.
PATRICi21188968. VBIBraJap65052_2742.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56808 Genomic DNA. Translation: CAA40143.1.
AJ005001 Genomic DNA. Translation: CAA06276.1.
BA000040 Genomic DNA. Translation: BAC48025.1.
PIRiS13330.
RefSeqiNP_769400.1. NC_004463.1.
WP_011085545.1. NZ_CP011360.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1DP6X-ray2.30A141-270[»]
1DP8X-ray2.50A141-270[»]
1DP9X-ray2.60A141-270[»]
1DRMX-ray2.40A141-270[»]
1LSVX-ray2.40A141-270[»]
1LSWX-ray2.20A141-270[»]
1LSXX-ray2.70A141-270[»]
1LT0X-ray2.40A141-270[»]
1XJ2X-ray2.00A154-269[»]
1XJ3X-ray1.90A154-269[»]
1XJ4X-ray1.80A/B151-269[»]
1XJ6X-ray1.90A/B151-269[»]
1Y28X-ray2.10A141-270[»]
2CMNX-ray2.30A141-270[»]
2OWHX-ray2.50A154-269[»]
2OWJX-ray2.50A154-269[»]
2VV6X-ray1.50A/B/C/D151-269[»]
2VV7X-ray1.81A/B/C/D151-269[»]
2VV8X-ray1.61A/B/C/D151-269[»]
4GCZX-ray2.30A/B257-505[»]
ProteinModelPortaliP23222.
SMRiP23222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.bll2760.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC48025; BAC48025; BAC48025.
GeneIDi1054108.
KEGGibja:bll2760.
PATRICi21188968. VBIBraJap65052_2742.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000252047.
InParanoidiP23222.
KOiK14986.
OMAiHARILIF.
OrthoDBiPOG091H0L6P.
PhylomeDBiP23222.

Enzyme and pathway databases

BRENDAi2.7.13.3. 929.

Miscellaneous databases

EvolutionaryTraceiP23222.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013767. PAS_fold.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 2 hits.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 2 hits.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 2 hits.
PS50112. PAS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFIXL_BRADU
AccessioniPrimary (citable) accession number: P23222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.