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Reviewed, UniProtKB/Swiss-Prot P23220 (AT2B1_PIG)

Last modified June 16, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Plasma membrane calcium-transporting ATPase 1
      Short name=PMCA1
    EC=3.6.3.8
Alternative name(s):
    Plasma membrane calcium pump isoform 1
Gene names
Name: ATP2B1
OrganismSus scrofa (Pig)
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

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Protein attributes

Sequence length1220 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.

Catalytic activity

ATP + H2O + Ca2+(Cis) = ADP + phosphate + Ca2+(Trans).

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12201220Plasma membrane calcium-transporting ATPase 1
PRO_0000046210

Regions

Topological domain1 – 9797Cytoplasmic Potential
Transmembrane98 – 11821 Potential
Topological domain119 – 15436Extracellular Potential
Transmembrane155 – 17521 Potential
Topological domain176 – 366191Cytoplasmic Potential
Transmembrane367 – 38620 Potential
Topological domain387 – 41933Extracellular Potential
Transmembrane420 – 43718 Potential
Topological domain438 – 852415Cytoplasmic Potential
Transmembrane853 – 87220 Potential
Topological domain873 – 88210Extracellular Potential
Transmembrane883 – 90321 Potential
Topological domain904 – 92320Cytoplasmic Potential
Transmembrane924 – 94623 Potential
Topological domain947 – 96418Extracellular Potential
Transmembrane965 – 98622 Potential
Topological domain987 – 100519Cytoplasmic Potential
Transmembrane1006 – 102722 Potential
Topological domain1028 – 103710Extracellular Potential
Transmembrane1038 – 105922 Potential
Topological domain1060 – 1220161Cytoplasmic Potential
Region1100 – 111718Calmodulin-binding subdomain A By similarity
Region1118 – 112710Calmodulin-binding subdomain B By similarity
Compositional bias296 – 2994Poly-Glu

Sites

Active site47514-aspartylphosphate intermediate By similarity
Metal binding7971Magnesium By similarity
Metal binding8011Magnesium By similarity

Amino acid modifications

Modified residue171Phosphoserine By similarity
Modified residue11161Phosphothreonine; by PKC By similarity
Modified residue11551Phosphoserine By similarity
Modified residue11651Phosphothreonine By similarity
Modified residue11821Phosphoserine By similarity
Modified residue12081Phosphoserine By similarity
Modified residue12111Phosphoserine By similarity
Modified residue12151Phosphoserine By similarity
Modified residue12181Phosphothreonine By similarity
Modified residue12191Phosphoserine By similarity

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Sequences

Sequence LengthMass (Da)Tools
P23220-1 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 3372FAF67F82401A

FASTA1,220134,710
        10         20         30         40         50         60 
MGDMANNSVA YGGVKNSLKE ANHDGDFGIT LADVRALMEL RSTDALRKIQ ESYGDVYGIC 

        70         80         90        100        110        120 
TRLKTSPVEG LSGNPADIER REAVFGKNFI PPKKPKTFLQ LVWEALQDVT LIILEIAAIV 

       130        140        150        160        170        180 
SLGLSFYQPP EGDNALCGEV SVGEEEGEGE TGWIEGAAIL LSVVCVVLVT AFNDWSKEKQ 

       190        200        210        220        230        240 
FRGLQSRIEQ EQKFTVIRGG QVIQIPVADI TVGDIAQVKY GDLLPADGIL IQGNDLKIDE 

       250        260        270        280        290        300 
SSLTGESDHV KKSLDKDPLL LSGTHVMEGS GRMVVTAVGI NSQTGIIFTL LGAGGEEEEK 

       310        320        330        340        350        360 
KDEKKKEKKN KKQDGAIENR NKAKAQDGAA MEMQPLKSEE GGDGDEKDKK KANLPKKEKS 

       370        380        390        400        410        420 
VLQGKLTKLA VQIGKAGLLM SAITVIILVL YFVIDTFWVQ KRPWLAECTP IYIQYFVKFF 

       430        440        450        460        470        480 
IIGVTVLVVA VPEGLPLAVT ISLAYSVKKM MKDNNLVRHL DACETMGNAT AICSDKTGTL 

       490        500        510        520        530        540 
TMNRMTVVQA YINEKHYKKI PEPEAIPPNI LSYLVTGISV NCAYTSKILP PEKEGGLPRH 

       550        560        570        580        590        600 
VGNKTECALL GLLLDLKRDY QDVRNEIPEE ALYKVYTFNS VRKSMSTVLK NSDGSYRIFS 

       610        620        630        640        650        660 
KGASEIILKK CFKILSANGE AKVFRPRDRD DIVKTVIEPM ASEGLRTICL AFRDFPAGEP 

       670        680        690        700        710        720 
EPEWDNENDI VTGLTCIAVV GIEDPVRPEV PDAIKKCQRA GITVRMVTGD NINTARAIAT 

       730        740        750        760        770        780 
KCGILHPGED FLCLEGKDFN RRIRNEKGEI EQERIDKIWP KLRVLARSSP TDKHTLVKGI 

       790        800        810        820        830        840 
IDSTVSDQRQ VVAVTGDGTN DGPALKKADV GFAMGIAGTD VAKEASDIIL TDDNFTSIVK 

       850        860        870        880        890        900 
AVMWGRNVYD SISKFLQFQL TVNVVAVIVA FTGACITQDS PLKAVQMLWV NLIMDTLASL 

       910        920        930        940        950        960 
ALATEPPTES LLLRKPYGRN KPLISRTMMK NILGHAFYQL VVVFTLLFAG EKFFDIDSGR 

       970        980        990       1000       1010       1020 
NAPLHAPPSE HYTIVFNTFV LMQLFNEINA RKIHGERNVF EGIFNNAIFC TIVLGTFVVQ 

      1030       1040       1050       1060       1070       1080 
IIIVQFGGKP FSCSELSIEQ WLWSIFLGMG TLLWGQLIST IPTSRLKFLK EAGHGTQKEE 

      1090       1100       1110       1120       1130       1140 
IPEEELAEDV EEIDHAEREL RRGQILWFRG LNRIQTQIRV VNAFRSSLYE GLEKPESRSS 

      1150       1160       1170       1180       1190       1200 
IHNFMTHPEF RIEDSEPHIP LIDDTDAEDD APTKRNCSPP PSPNKNNNAV DSGIYLTIEM 

      1210       1220 
NKSATSSSPG SPLHSLETSL

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References

[1]"Molecular cloning and sequencing of the plasma-membrane Ca2+ pump of pig smooth muscle."
de Jaegere S., Wuytack F., Eggermont J.A., Verboomen H., Casteels R.
Biochem. J. 271:655-660(1990) [PubMed: 2147100] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Stomach smooth muscle.

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Cross-references

Sequence databases

X53456 mRNA. Translation: CAA37536.1.
PIRS13057.
RefSeqNP_999517.1.
UniGeneSsc.55555

3D structure databases

HSSPHSSP built from PDB template 1EUL based on UniProtKB P04191.
ModBaseSearch...

Genome annotation databases

GeneID397636.
KEGGssc:397636.

Phylogenomic databases

HOVERGENP23220.

Enzyme and pathway databases

BRENDA3.6.3.8. 249.

Family and domain databases

InterProIPR008250. ATPase_P-typ_ATPase-assoc-reg.
IPR006408. ATPase_P-typ_Ca-transp_PMCA.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_phosphor_site.
IPR005834. Dehalogen-like_hydro.
[Graphical view]
PANTHERPTHR11939. ATPase_P. 1 hit.
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
TIGRFAMsTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 4 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameAT2B1_PIG
AccessionPrimary (citable) accession number: P23220
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 16, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents