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Reviewed, UniProtKB/Swiss-Prot P23202 (URE2_YEAST)

Last modified November 25, 2008. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Protein URE2
Gene names
Name: URE2
Ordered Locus Names: YNL229C
ORF Names: N1165
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length354 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Plays an important role in the cellular response to the nitrogen source. URE2 gene plays a major part in the repression of GLN1 and GDH2 genes by glutamine, and is required for the inactivation of glutamine synthetase. URE2 gene product may catalytically inactivate GLN3 in response to an increase in the intracellular concentration of glutamine.

Subunit structure

Homodimer.

Miscellaneous

Can provoque the non-Mendelian trait [URE3]. The [URE3] element of S.cerevisiae is due to the propagation of a transmissible form of the protein URE2. The infectivity of URE2 is thought to originate from a conformational change of the normal form of the prion protein. This conformational change generates a form of URE2 that assembles into amyloid fibrils.

Present with 7060 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GST superfamily.

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 354354Protein URE2
PRO_0000186013

Regions

Domain112 – 19685GST N-terminal
Domain205 – 354150GST C-terminal

Amino acid modifications

Modified residue2821Phosphotyrosine
Modified residue2831Phosphoserine
Modified residue2871Phosphothreonine

Experimental info

Mutagenesis3131F → S: Destroys protein function

Secondary structure

.................................... 354
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23202-1 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 2C628976034B0F1C

FASTA35440,271
        10         20         30         40         50         60 
MMNNNGNQVS NLSNALRQVN IGNRNSNTTT DQSNINFEFS TGVNNNNNNN SSSNNNNVQN 

        70         80         90        100        110        120 
NNSGRNGSQN NDNENNIKNT LEQHRQQQQA FSDMSHVEYS RITKFFQEQP LEGYTLFSHR 

       130        140        150        160        170        180 
SAPNGFKVAI VLSELGFHYN TIFLDFNLGE HRAPEFVSVN PNARVPALID HGMDNLSIWE 

       190        200        210        220        230        240 
SGAILLHLVN KYYKETGNPL LWSDDLADQS QINAWLFFQT SGHAPMIGQA LHFRYFHSQK 

       250        260        270        280        290        300 
IASAVERYTD EVRRVYGVVE MALAERREAL VMELDTENAA AYSAGTTPMS QSRFFDYPVW 

       310        320        330        340        350 
LVGDKLTIAD LAFVPWNNVV DRIGINIKIE FPEVYKWTKH MMRRPAVIKA LRGE 

« Hide

References

« Hide 'large scale' references
[1]"The URE2 gene product of Saccharomyces cerevisiae plays an important role in the cellular response to the nitrogen source and has homology to glutathione s-transferases."
Coschigano P.W., Magasanik B.
Mol. Cell. Biol. 11:822-832(1991) [PubMed: 1990286] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Conservation of a portion of the S. cerevisiae Ure2p prion domain that interacts with the full-length protein."
Edskes H.K., Wickner R.B.
Proc. Natl. Acad. Sci. U.S.A. 99:16384-16391(2002) [PubMed: 12177423] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 14085 / CBS 3093 / IFO 1997 / NRRL Y-12657, ATCC 9804 / CBS 400 / DSM 70478 / IFO 0210 / JCM 2220, Boots, CBS 2087, CBS 4734, CBS 5112, CBS 5287, CBS 7957, Chevalieri / ATCC 10604 / CBS 405 / IFO 0258 / NRRL Y-1546, Fleischmann, McPhie Sourdough, SAF, Sigma 1278B, Wyeast#1007, YJM 143, YJM 280, YJM 320, YJM 326 and YJM 415.
[3]"The DNA sequence of cosmid 14-5 from chromosome XIV reveals 21 open reading frames including a novel gene encoding a globin-like domain."
Pandolfo D., de Antoni A., Lanfranchi G., Valle G.
Yeast 12:1071-1076(1996) [PubMed: 8896273] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed: 9169873] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-282; SER-283 AND THR-287, MASS SPECTROMETRY.
[7]"Structure of the globular region of the prion protein Ure2 from the yeast Saccharomyces cerevisiae."
Bousset L., Belrhali H., Janin J., Melki R., Morera S.
Structure 9:39-46(2001) [PubMed: 11342133] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 100-353.
+Additional computationally mapped references.

Web resources

Protein Spotlight

Mad yeast disease - Issue 47 of June 2004

Cross-references

Sequence databases

M35268 Genomic DNA. Translation: AAA35201.1.
AF525174 Genomic DNA. Translation: AAM93167.1.
AF525175 Genomic DNA. Translation: AAM93168.1.
AF525176 Genomic DNA. Translation: AAM93169.1.
AF525177 Genomic DNA. Translation: AAM93170.1.
AF525178 Genomic DNA. Translation: AAM93171.1.
AF525179 Genomic DNA. Translation: AAM93172.1.
AF525180 Genomic DNA. Translation: AAM93173.1.
AF525181 Genomic DNA. Translation: AAM93174.1.
AF525182 Genomic DNA. Translation: AAM93175.1.
AF525183 Genomic DNA. Translation: AAM93176.1.
AF525184 Genomic DNA. Translation: AAM93177.1.
AF525185 Genomic DNA. Translation: AAM93178.1.
AF525186 Genomic DNA. Translation: AAM93179.1.
AF525187 Genomic DNA. Translation: AAM93180.1.
AF525188 Genomic DNA. Translation: AAM93181.1.
AF525189 Genomic DNA. Translation: AAM93182.1.
AF525190 Genomic DNA. Translation: AAM93183.1.
AF525191 Genomic DNA. Translation: AAM93184.1.
AF525192 Genomic DNA. Translation: AAM93185.1.
Z69381 Genomic DNA. Translation: CAA93369.1.
Z71505 Genomic DNA. Translation: CAA96134.1.
PIRA39609.
RefSeqNP_014170.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1G6WX-ray2.50A/B/C/D94-354[»]
1G6YX-ray2.80A/B94-354[»]
1HQOX-ray2.30A/B97-354[»]
1JZRX-ray2.90A/B/C/D95-354[»]
1K0AX-ray2.50A/B95-354[»]
1K0BX-ray2.50A/B/C/D95-354[»]
1K0CX-ray2.50A/B/C/D95-354[»]
1K0DX-ray2.20A/B/C/D95-354[»]
DisProtDP00353.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1308N.
IntActP23202.

Proteomic databases

PeptideAtlasP23202.

Genome annotation databases

EnsemblYNL229C. Saccharomyces cerevisiae. [Contig view]
GeneID855492.
GenomeReviewsGene locus YNL229C in contig Y13139_GR.
KEGGsce:YNL229C.
NMPDRfig|4932.3.peg.5235.

Organism-specific databases

CYGDYNL229c.
SGDS000005173. URE2.
Yeast-GFPSearch...

Phylogenomic databases

HOGENOMP23202.

Gene expression databases

GermOnlineYNL229C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004046. GST_C.
IPR004045. GST_N.
IPR017298. Prion_URE2.
IPR012335. Thioredoxin_fold.
[Graphical view]
Gene3DG3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PfamPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
PIRSFPIRSF037861. Prion_URE2. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

LinkHubP23202.
NextBio979478.

Entry information

Entry nameURE2_YEAST
AccessionPrimary (citable) accession number: P23202
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 25, 2008
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents