Chromobox protein homolog 3 - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot P23198 (CBX3_MOUSE)

Last modified June 16, 2009. Version 84. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Chromobox protein homolog 3
Alternative name(s):
    Heterochromatin protein 1 homolog gamma
      Short name=HP1 gamma
    Modifier 2 protein
    M32

Gene names

Name:

Cbx3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Probably involved in the repression of many genes located in euchromatin, such as E2F1, MYC and CDC25A. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins By similarity.
Subunit structure	Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2 By similarity. Interacts with LBR, INCENP, TRIM28/TIF1B and SP100. Interacts with TIF1/TIF1A. Interacts with MIS12 and C20orf127 By similarity. Can interact directly with CBX5 via the chromoshadow domain By similarity. Interacts with SUV420H1 and SUV420H2.
Subcellular location	Nucleus. Note: May be associated with microtubules and mitotic poles during mitosis Potential. Associates with euchromatin and is largely excluded from constitutive heterochromatin.
Post-translational modification	Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis By similarity.
Sequence similarities	Contains 2 chromo domains.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Molecular function	Chromatin regulator Repressor
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	chromatin assembly or disassembly Inferred from electronic annotation. Source: InterPro chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription, DNA-dependent Inferred from direct assay. Source: MGI transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromocenter Inferred from direct assay. Source: MGI condensed chromosome, centromeric region Inferred from direct assay. Source: UniProtKB nuclear centromeric heterochromatin Inferred from direct assay. Source: UniProtKB nuclear envelope Inferred from direct assay. Source: UniProtKB
Molecular function	chromatin binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct transcription repressor activity Inferred from direct assay. Source: MGI
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
HIST1H3A	P68431	1	EBI-78162,EBI-79722	From a different organism.
TRIM28	Q13263	1	EBI-78162,EBI-78139	From a different organism.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 183

183

Chromobox protein homolog 3

PRO_0000080204

Regions

Domain

30 – 88

Chromo 1

Domain

121 – 179

Chromo 2; shadow subtype

Amino acid modifications

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine Ref.8

Modified residue

Phosphoserine By similarity

Modified residue

176

Phosphoserine Ref.7

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Sequences

Sequence

Length

Mass (Da)

Tools

P23198-1 [UniParc].

Last modified July 11, 2002. Version 2.
Checksum: 2FF3AD53B883E27E
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FASTA

183

20,855

        10         20         30         40         50         60 
MASNKTTLQK MGKKQNGKSK KVEEAEPEEF VVEKVLDRRV VNGKVEYFLK WKGFTDADNT 

        70         80         90        100        110        120 
WEPEENLDCP ELIEDFLNSQ KAGKEKDGTK RKSLSDSESD DSKSKKKRDA ADKPRGFARG 

       130        140        150        160        170        180 
LDPERIIGAT DSSGELMFLM KWKDSDEADL VLAKEANMKC PQIVIAFYEE RLTWHSCPED 


EAQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The gene and pseudogenes of Cbx3." Jones D.O., Mattei M.-G., Horsley D., Cowell I.G., Singh P.B. Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"A sequence motif found in a Drosophila heterochromatin protein is conserved in animals and plants." Singh P.B., Miller J.R., Pearce J., Kothary R., Burton R.D., Paro R., James T.C., Gaunt S.J. Nucleic Acids Res. 19:789-794(1991) [PubMed: 1708124] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 11-183. Strain: C57BL/6. Tissue: Embryo.
[3]	Lubec G., Yang J.W., Zigmond M. Submitted (JUL-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 160-171. Tissue: Brain.
[4]	"M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchromatin within interphase nuclei and is largely excluded from constitutive heterochromatin." Horsley D., Hutchings A., Butcher G.W., Singh P.B. Cytogenet. Cell Genet. 73:308-311(1996) [PubMed: 8751383] [Abstract] Cited for: SUBCELLULAR LOCATION.
[5]	"A possible involvement of TIF1 alpha and TIF1 beta in the epigenetic control of transcription by nuclear receptors." le Douarin B., Nielsen A.L., Garnier J.-M., Ichinose H., Jeanmougin F., Losson R., Chambon P. EMBO J. 15:6701-6715(1996) [PubMed: 8978696] [Abstract] Cited for: INTERACTION WITH TIF1A.
[6]	"A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin." Schotta G., Lachner M., Sarma K., Ebert A., Sengupta R., Reuter G., Reinberg D., Jenuwein T. Genes Dev. 18:1251-1262(2004) [PubMed: 15145825] [Abstract] Cited for: INTERACTION WITH SUV420H1 AND SUV420H2.
[7]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed: 18630941] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-93 AND SER-95, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	X56683 mRNA. Translation: CAA40012.1. AJ278617, AJ278618, AJ278619 Genomic DNA. Translation: CAC42944.1.
IPI	IPI00129468.
PIR	S26846.
UniGene	Mm.280968 Mm.393602 Mm.394056 Mm.458234
3D structure databases
HSSP	HSSP built from PDB template 1DZ1 based on UniProtKB P23197.
SMR	P23198. Positions 36-87, 113-178.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:28136N.
IntAct	P23198. 4 interactions.
PTM databases
PhosphoSite	P23198.
Proteomic databases
PRIDE	P23198.
Genome annotation databases
Ensembl	ENSMUSG00000029836. Mus musculus. [Contig view] ENSMUSG00000059647. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:108515. Cbx3.
Phylogenomic databases
HOGENOM	P23198.
HOVERGEN	P23198.
Gene expression databases
Bgee	P23198.
CleanEx	MM_CBX3.
GermOnline	ENSMUSG00000029836. Mus musculus. ENSMUSG00000057886. Mus musculus. ENSMUSG00000059647. Mus musculus.
Family and domain databases
InterPro	IPR017984. Chromo_dom_subgr. IPR008251. Chromo_shadow. IPR018125. Chromo_shadow_sbgrp. IPR000953. Chromodomain. [Graphical view]
Pfam	PF00385. Chromo. 1 hit. PF01393. Chromo_shadow. 1 hit. [Graphical view]
PRINTS	PR00504. CHROMODOMAIN.
SMART	SM00298. CHROMO. 2 hits. SM00300. ChSh. 1 hit. [Graphical view]
PROSITE	PS00598. CHROMO_1. 1 hit. PS50013. CHROMO_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

CBX3_MOUSE

Accession

Primary (citable) accession number: P23198
Secondary accession number(s): Q921C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	July 11, 2002
Last modified:	June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents