Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Chromobox protein homolog 3

Gene

Cbx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Probably involved in the repression of many genes located in euchromatin, such as E2F1, MYC and CDC25A. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1 (PubMed:24413057). Mediates the recruitement of NIPBL to sites of DNA damage at double-strand breaks (DSBs) (By similarity).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Repressor
Biological processBiological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 3
Alternative name(s):
Heterochromatin protein 1 homolog gamma
Short name:
HP1 gamma
M32
Modifier 2 protein
Gene namesi
Name:Cbx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:108515 Cbx3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000802042 – 183Chromobox protein homolog 3Add BLAST182

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei5N6-acetyllysine; alternateCombined sources1
Cross-linki5Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei10N6-acetyllysine; alternateBy similarity1
Cross-linki10Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Cross-linki21Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei44N6-acetyllysineCombined sources1
Modified residuei50N6-acetyllysineBy similarity1
Modified residuei93PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei97PhosphoserineBy similarity1
Modified residuei99PhosphoserineBy similarity1
Cross-linki103Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei176PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP23198
MaxQBiP23198
PaxDbiP23198
PeptideAtlasiP23198
PRIDEiP23198

2D gel databases

REPRODUCTION-2DPAGEiIPI00129468

PTM databases

iPTMnetiP23198
PhosphoSitePlusiP23198

Expressioni

Gene expression databases

CleanExiMM_CBX3

Interactioni

Subunit structurei

Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B and SP100. Interacts with TIF1/TIF1A. Interacts with MIS12 and DSN1. Can interact directly with CBX5 via the chromoshadow domain. Interacts with KMT5B and KMT5C. Interacts with POGZ. Interacts with CHAMP1. Interacts with ASXL1. The large PER complex involved in the histone methylation is composed of at least PER2, CBX3, TRIM28, SUV39H1 and/or SUV39H2; CBX3 mediates the formation of the complex. Interacts with INCENP. Interacts with NIPBL (via PxVxL motif) (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIM28Q132632EBI-78162,EBI-78139From Homo sapiens.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-28136N
IntActiP23198, 17 interactors
MINTiP23198
STRINGi10090.ENSMUSP00000080177

Structurei

3D structure databases

ProteinModelPortaliP23198
SMRiP23198
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 88Chromo 1PROSITE-ProRule annotationAdd BLAST59
Domaini121 – 179Chromo 2; shadow subtypePROSITE-ProRule annotationAdd BLAST59

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1911 Eukaryota
ENOG4111JKD LUCA
HOGENOMiHOG000220852
HOVERGENiHBG000400
InParanoidiP23198

Family and domain databases

CDDicd00024 CHROMO, 1 hit
InterProiView protein in InterPro
IPR038033 CBX3
IPR016197 Chromo-like_dom_sf
IPR000953 Chromo/chromo_shadow_dom
IPR017984 Chromo_dom_subgr
IPR023780 Chromo_domain
IPR008251 Chromo_shadow_dom
IPR023779 Chromodomain_CS
PANTHERiPTHR22812:SF110 PTHR22812:SF110, 1 hit
PfamiView protein in Pfam
PF00385 Chromo, 1 hit
PF01393 Chromo_shadow, 1 hit
PRINTSiPR00504 CHROMODOMAIN
SMARTiView protein in SMART
SM00298 CHROMO, 2 hits
SM00300 ChSh, 1 hit
SUPFAMiSSF54160 SSF54160, 2 hits
PROSITEiView protein in PROSITE
PS00598 CHROMO_1, 1 hit
PS50013 CHROMO_2, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23198-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNKTTLQK MGKKQNGKSK KVEEAEPEEF VVEKVLDRRV VNGKVEYFLK
60 70 80 90 100
WKGFTDADNT WEPEENLDCP ELIEDFLNSQ KAGKEKDGTK RKSLSDSESD
110 120 130 140 150
DSKSKKKRDA ADKPRGFARG LDPERIIGAT DSSGELMFLM KWKDSDEADL
160 170 180
VLAKEANMKC PQIVIAFYEE RLTWHSCPED EAQ
Length:183
Mass (Da):20,855
Last modified:July 11, 2002 - v2
Checksum:i2FF3AD53B883E27E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56683 mRNA Translation: CAA40012.1
AJ278617, AJ278618, AJ278619 Genomic DNA Translation: CAC42944.1
PIRiS26846
UniGeneiMm.280968
Mm.393602
Mm.458234
Mm.490361

Similar proteinsi

Entry informationi

Entry nameiCBX3_MOUSE
AccessioniPrimary (citable) accession number: P23198
Secondary accession number(s): Q921C4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: July 11, 2002
Last modified: April 25, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health