P23194 (URIC_DROVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uricase
EC=1.7.3.3

Alternative name(s):
Urate oxidase

Gene names

Name:	Uro
Synonyms:	UO

Organism

Drosophila virilis (Fruit fly)

Taxonomic identifier

7244 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2.
Enzyme regulation	Repressed by 20-hydroxyecdysone.
Pathway	Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.
Subcellular location	Peroxisome.
Tissue specificity	Malpighian tubules.
Developmental stage	Third instar larvae and adult.
Sequence similarities	Belongs to the uricase family.

Ontologies

Keywords
Biological process	Purine metabolism
Cellular component	Peroxisome
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	urate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Uricase		PRO_0000165994
Regions
	Region	80 – 81	2	Substrate binding By similarity
	Region	269 – 270	2	Substrate binding By similarity
	Motif	340 – 342	3	Microbody targeting signal Potential
Sites
	Active site	221	1	Charge relay system By similarity
	Active site	270	1	Charge relay system By similarity
	Binding site	221	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23194 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 4DE10D5485294723	FASTA	342	38,724
	10 20 30 40 50 60 MFATPLRQLS SLKRSGIAGQ EQAQLYEITN KGYGKDAVKV MHINRKGPVH SIQELEVGTH 70 80 90 100 110 120 LKLYSNKDYM LGNNSDVVAT DSQKNTVYLL AKKHGIESPE KFALILAKHF LSTYAHVEEV 130 140 150 160 170 180 HVHVEAYPWQ RMTQDVSDNI GKGYCENNCN SRSNGNCQLH NHAFIFTPTA HDYCDVILTR 190 200 210 220 230 240 QDPKQTVISG IKGLRVLKTT QSSFVNFVDD EFRTLADQYD RIFSTVVECS WEYSDTESVN 250 260 270 280 290 300 FLHAWETVKD IVVRNFAGDP SVGIPSPSVQ HTLYLSEKQV LDVLPQVSVV SMTMPNKHYF 310 320 330 340 NFDTKPFQQL VPGENNEVFI PTDKPHGTIY AQLSRKSLKS HL

References

[1]	Wallrath L.L. Submitted (JAN-1991) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57114 Genomic DNA. Translation: CAA40397.1.
PIR	S29133.
3D structure databases
ProteinModelPortal	P23194.
ModBase	Search...
Protein-protein interaction databases
STRING	P23194.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
FlyBase	FBgn0013101. Dvir\Uro.
Phylogenomic databases
GeneTree	EMGT00050000017624.
InParanoid	P23194.
OrthoDB	EOG4ZW3SM.
Family and domain databases
InterPro	IPR002042. Uricase. IPR019842. Uricase_CS. [Graphical view]
Gene3D	G3DSA:3.10.270.10. Uricase. 1 hit.
PANTHER	PTHR10395:SF1. Uricase. 1 hit.
Pfam	PF01014. Uricase. 2 hits. [Graphical view]
PIRSF	PIRSF000241. Urate_oxidase. 1 hit.
PRINTS	PR00093. URICASE.
TIGRFAMs	TIGR03383. Urate_oxi. 1 hit.
PROSITE	PS00366. URICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URIC_DROVI

Accession

Primary (citable) accession number: P23194

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project