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Protein

Transcription elongation factor A protein 1

Gene

TCEA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 299TFIIS-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
SIGNORiP23193.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 1
Alternative name(s):
Transcription elongation factor S-II protein 1
Transcription elongation factor TFIIS.o
Gene namesi
Name:TCEA1
Synonyms:GTF2S, TFIIS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:11612. TCEA1.

Subcellular locationi

GO - Cellular componenti

  • nucleolus Source: HPA
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
  • transcription factor TFIID complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.

Organism-specific databases

DisGeNETi6917.
OpenTargetsiENSG00000187735.
PharmGKBiPA36371.

Polymorphism and mutation databases

DMDMi1174652.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214461 – 301Transcription elongation factor A protein 1Add BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Cross-linki55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei57PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP23193.
MaxQBiP23193.
PaxDbiP23193.
PeptideAtlasiP23193.
PRIDEiP23193.

PTM databases

iPTMnetiP23193.
PhosphoSitePlusiP23193.

Miscellaneous databases

PMAP-CutDBP23193.

Expressioni

Gene expression databases

BgeeiENSG00000187735.
CleanExiHS_TCEA1.
ExpressionAtlasiP23193. baseline and differential.
GenevisibleiP23193. HS.

Organism-specific databases

HPAiHPA043786.

Interactioni

Subunit structurei

Interacts with EAF2 (By similarity). Associates with UBR5 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
LEO1Q8WVC04EBI-2608271,EBI-932432
PAF1Q8N7H54EBI-2608271,EBI-2607770

Protein-protein interaction databases

BioGridi112779. 71 interactors.
DIPiDIP-48480N.
IntActiP23193. 12 interactors.
MINTiMINT-3009864.
STRINGi9606.ENSP00000428426.

Structurei

Secondary structure

1301
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi139 – 151Combined sources13
Helixi152 – 156Combined sources5
Helixi157 – 161Combined sources5
Helixi165 – 180Combined sources16
Beta strandi182 – 184Combined sources3
Helixi185 – 198Combined sources14
Helixi204 – 211Combined sources8
Helixi217 – 222Combined sources6
Turni225 – 227Combined sources3
Beta strandi264 – 266Combined sources3
Beta strandi271 – 276Combined sources6
Beta strandi278 – 283Combined sources6
Beta strandi286 – 294Combined sources9
Beta strandi297 – 299Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TFINMR-A252-301[»]
3NDQX-ray1.93A131-232[»]
5IY6electron microscopy7.20U1-301[»]
5IY7electron microscopy8.60U1-301[»]
5IY8electron microscopy7.90U1-301[»]
5IYAelectron microscopy5.40U1-301[»]
5IYBelectron microscopy3.90U1-301[»]
5IYCelectron microscopy3.90U1-301[»]
ProteinModelPortaliP23193.
SMRiP23193.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23193.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 80TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST78
Domaini140 – 256TFIIS centralPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Belongs to the TFS-II family.Curated
Contains 1 TFIIS central domain.PROSITE-ProRule annotation
Contains 1 TFIIS N-terminal domain.PROSITE-ProRule annotation
Contains 1 TFIIS-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 299TFIIS-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiP23193.
KOiK03145.
OMAiMSKISMA.
OrthoDBiEOG091G10EJ.
PhylomeDBiP23193.
TreeFamiTF314970.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P23193-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDEVVRFAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV
60 70 80 90 100
NAIRKQSTDE EVTSLAKSLI KSWKKLLDGP STEKDLDEKK KEPAITSQNS
110 120 130 140 150
PEAREESTSS GNVSNRKDET NARDTYVSSF PRAPSTSDSV RLKCREMLAA
160 170 180 190 200
ALRTGDDYIA IGADEEELGS QIEEAIYQEI RNTDMKYKNR VRSRISNLKD
210 220 230 240 250
AKNPNLRKNV LCGNIPPDLF ARMTAEEMAS DELKEMRKNL TKEAIREHQM
260 270 280 290 300
AKTGGTQTDL FTCGKCKKKN CTYTQVQTRS ADEPMTTFVV CNECGNRWKF

C
Length:301
Mass (Da):33,970
Last modified:November 1, 1995 - v2
Checksum:i8A685107A56D2DA1
GO
Isoform 2 (identifier: P23193-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     22-42: Missing.

Show »
Length:280
Mass (Da):31,664
Checksum:i31833641990136EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127V → L in AAA61138 (PubMed:1378807).Curated1
Sequence conflicti141R → Q in CAA51940 (PubMed:8112616).Curated1
Sequence conflicti205N → Y in CAA51940 (PubMed:8112616).Curated1
Sequence conflicti237R → W in CAA51940 (PubMed:8112616).Curated1
Sequence conflicti285M → V in CAA51940 (PubMed:8112616).Curated1
Sequence conflicti300F → I in AC100821 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00640922 – 42Missing in isoform 2. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62585 mRNA. Translation: CAA44470.1.
X57198 mRNA. Translation: CAA40484.1.
M81601 mRNA. Translation: AAA61138.1.
X73534 Genomic DNA. Translation: CAA51940.1.
AK290454 mRNA. Translation: BAF83143.1.
CR542221 mRNA. Translation: CAG47017.1.
BT019995 mRNA. Translation: AAV38798.1.
AC100821 Genomic DNA. No translation available.
BC072460 mRNA. Translation: AAH72460.1.
CCDSiCCDS47857.1. [P23193-2]
CCDS47858.1. [P23193-1]
PIRiS17361.
S26831.
S34159.
RefSeqiNP_006747.1. NM_006756.3. [P23193-1]
NP_958845.1. NM_201437.2. [P23193-2]
UniGeneiHs.491745.

Genome annotation databases

EnsembliENST00000396401; ENSP00000395483; ENSG00000187735. [P23193-2]
ENST00000521604; ENSP00000428426; ENSG00000187735. [P23193-1]
GeneIDi6917.
KEGGihsa:6917.
UCSCiuc003xru.5. human. [P23193-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62585 mRNA. Translation: CAA44470.1.
X57198 mRNA. Translation: CAA40484.1.
M81601 mRNA. Translation: AAA61138.1.
X73534 Genomic DNA. Translation: CAA51940.1.
AK290454 mRNA. Translation: BAF83143.1.
CR542221 mRNA. Translation: CAG47017.1.
BT019995 mRNA. Translation: AAV38798.1.
AC100821 Genomic DNA. No translation available.
BC072460 mRNA. Translation: AAH72460.1.
CCDSiCCDS47857.1. [P23193-2]
CCDS47858.1. [P23193-1]
PIRiS17361.
S26831.
S34159.
RefSeqiNP_006747.1. NM_006756.3. [P23193-1]
NP_958845.1. NM_201437.2. [P23193-2]
UniGeneiHs.491745.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TFINMR-A252-301[»]
3NDQX-ray1.93A131-232[»]
5IY6electron microscopy7.20U1-301[»]
5IY7electron microscopy8.60U1-301[»]
5IY8electron microscopy7.90U1-301[»]
5IYAelectron microscopy5.40U1-301[»]
5IYBelectron microscopy3.90U1-301[»]
5IYCelectron microscopy3.90U1-301[»]
ProteinModelPortaliP23193.
SMRiP23193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112779. 71 interactors.
DIPiDIP-48480N.
IntActiP23193. 12 interactors.
MINTiMINT-3009864.
STRINGi9606.ENSP00000428426.

PTM databases

iPTMnetiP23193.
PhosphoSitePlusiP23193.

Polymorphism and mutation databases

DMDMi1174652.

Proteomic databases

EPDiP23193.
MaxQBiP23193.
PaxDbiP23193.
PeptideAtlasiP23193.
PRIDEiP23193.

Protocols and materials databases

DNASUi6917.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000396401; ENSP00000395483; ENSG00000187735. [P23193-2]
ENST00000521604; ENSP00000428426; ENSG00000187735. [P23193-1]
GeneIDi6917.
KEGGihsa:6917.
UCSCiuc003xru.5. human. [P23193-1]

Organism-specific databases

CTDi6917.
DisGeNETi6917.
GeneCardsiTCEA1.
H-InvDBHIX0057264.
HGNCiHGNC:11612. TCEA1.
HPAiHPA043786.
MIMi601425. gene.
neXtProtiNX_P23193.
OpenTargetsiENSG00000187735.
PharmGKBiPA36371.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1105. Eukaryota.
COG1594. LUCA.
GeneTreeiENSGT00390000017794.
HOGENOMiHOG000195015.
HOVERGENiHBG055022.
InParanoidiP23193.
KOiK03145.
OMAiMSKISMA.
OrthoDBiEOG091G10EJ.
PhylomeDBiP23193.
TreeFamiTF314970.

Enzyme and pathway databases

ReactomeiR-HSA-112382. Formation of RNA Pol II elongation complex.
R-HSA-112387. Elongation arrest and recovery.
R-HSA-167152. Formation of HIV elongation complex in the absence of HIV Tat.
R-HSA-167200. Formation of HIV-1 elongation complex containing HIV-1 Tat.
R-HSA-167238. Pausing and recovery of Tat-mediated HIV elongation.
R-HSA-167243. Tat-mediated HIV elongation arrest and recovery.
R-HSA-167246. Tat-mediated elongation of the HIV-1 transcript.
R-HSA-167287. HIV elongation arrest and recovery.
R-HSA-167290. Pausing and recovery of HIV elongation.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6781823. Formation of TC-NER Pre-Incision Complex.
R-HSA-6781827. Transcription-Coupled Nucleotide Excision Repair (TC-NER).
R-HSA-6782135. Dual incision in TC-NER.
R-HSA-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER.
R-HSA-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-HSA-75955. RNA Polymerase II Transcription Elongation.
SIGNORiP23193.

Miscellaneous databases

EvolutionaryTraceiP23193.
GeneWikiiTCEA1.
GenomeRNAii6917.
PMAP-CutDBP23193.
PROiP23193.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000187735.
CleanExiHS_TCEA1.
ExpressionAtlasiP23193. baseline and differential.
GenevisibleiP23193. HS.

Family and domain databases

Gene3Di1.10.472.30. 1 hit.
1.20.930.10. 1 hit.
InterProiIPR016492. TF_IIS-rel.
IPR003617. TFIIS/CRSP70_N_sub.
IPR003618. TFIIS_cen_dom.
IPR017923. TFIIS_N.
IPR006289. TFSII.
IPR001222. Znf_TFIIS.
[Graphical view]
PfamiPF08711. Med26. 1 hit.
PF01096. TFIIS_C. 1 hit.
PF07500. TFIIS_M. 1 hit.
[Graphical view]
PIRSFiPIRSF006704. TF_IIS. 1 hit.
SMARTiSM00510. TFS2M. 1 hit.
SM00509. TFS2N. 1 hit.
SM00440. ZnF_C2C2. 1 hit.
[Graphical view]
SUPFAMiSSF46942. SSF46942. 1 hit.
SSF47676. SSF47676. 1 hit.
TIGRFAMsiTIGR01385. TFSII. 1 hit.
PROSITEiPS51321. TFIIS_CENTRAL. 1 hit.
PS51319. TFIIS_N. 1 hit.
PS00466. ZF_TFIIS_1. 1 hit.
PS51133. ZF_TFIIS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCEA1_HUMAN
AccessioniPrimary (citable) accession number: P23193
Secondary accession number(s): A6NF25
, A8K339, Q15563, Q6FG87
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 179 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.