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Protein

Transcription elongation factor A protein 1

Gene

TCEA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.

Miscellaneous

S-II binds to RNA-polymerase II in the absence of transcription.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 299TFIIS-typePROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6781823 Formation of TC-NER Pre-Incision Complex
R-HSA-6781827 Transcription-Coupled Nucleotide Excision Repair (TC-NER)
R-HSA-6782135 Dual incision in TC-NER
R-HSA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-75955 RNA Polymerase II Transcription Elongation
SIGNORiP23193

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription elongation factor A protein 1
Alternative name(s):
Transcription elongation factor S-II protein 1
Transcription elongation factor TFIIS.o
Gene namesi
Name:TCEA1
Synonyms:GTF2S, TFIIS
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000187735.12
HGNCiHGNC:11612 TCEA1
MIMi601425 gene
neXtProtiNX_P23193

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1.1 Publication

Organism-specific databases

DisGeNETi6917
OpenTargetsiENSG00000187735
PharmGKBiPA36371

Polymorphism and mutation databases

DMDMi1174652

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001214461 – 301Transcription elongation factor A protein 1Add BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Cross-linki55Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei57PhosphoserineCombined sources1
Modified residuei81PhosphoserineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Modified residuei100PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP23193
MaxQBiP23193
PaxDbiP23193
PeptideAtlasiP23193
PRIDEiP23193

PTM databases

iPTMnetiP23193
PhosphoSitePlusiP23193

Miscellaneous databases

PMAP-CutDBiP23193

Expressioni

Gene expression databases

BgeeiENSG00000187735
CleanExiHS_TCEA1
ExpressionAtlasiP23193 baseline and differential
GenevisibleiP23193 HS

Organism-specific databases

HPAiHPA043786

Interactioni

Subunit structurei

Interacts with EAF2 (By similarity). Associates with UBR5 and forms a transcription regulatory complex made of CDK9, RNAP II, UBR5 and TFIIS/TCEA1 that can stimulate target gene transcription (e.g. gamma fibrinogen/FGG) by recruiting their promoters.By similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi112779, 74 interactors
DIPiDIP-48480N
IntActiP23193, 23 interactors
STRINGi9606.ENSP00000428426

Structurei

Secondary structure

1301
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi139 – 151Combined sources13
Helixi152 – 156Combined sources5
Helixi157 – 161Combined sources5
Helixi165 – 180Combined sources16
Beta strandi182 – 184Combined sources3
Helixi185 – 198Combined sources14
Helixi204 – 211Combined sources8
Helixi217 – 222Combined sources6
Turni225 – 227Combined sources3
Beta strandi264 – 266Combined sources3
Beta strandi271 – 276Combined sources6
Beta strandi278 – 283Combined sources6
Beta strandi286 – 294Combined sources9
Beta strandi297 – 299Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1TFINMR-A252-301[»]
3NDQX-ray1.93A131-232[»]
5IY6electron microscopy7.20U1-301[»]
5IY7electron microscopy8.60U1-301[»]
5IY8electron microscopy7.90U1-301[»]
5IYAelectron microscopy5.40U1-301[»]
5IYBelectron microscopy3.90U1-301[»]
5IYCelectron microscopy3.90U1-301[»]
ProteinModelPortaliP23193
SMRiP23193
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23193

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 80TFIIS N-terminalPROSITE-ProRule annotationAdd BLAST78
Domaini140 – 256TFIIS centralPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Belongs to the TFS-II family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri259 – 299TFIIS-typePROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1105 Eukaryota
COG1594 LUCA
GeneTreeiENSGT00390000017794
HOGENOMiHOG000195015
HOVERGENiHBG055022
InParanoidiP23193
KOiK03145
OMAiKKAQVPM
OrthoDBiEOG091G10EJ
PhylomeDBiP23193
TreeFamiTF314970

Family and domain databases

Gene3Di1.10.472.30, 1 hit
1.20.930.10, 1 hit
InterProiView protein in InterPro
IPR035100 TF_IIS-typ
IPR003617 TFIIS/CRSP70_N_sub
IPR035441 TFIIS/LEDGF_dom_sf
IPR003618 TFIIS_cen_dom
IPR036575 TFIIS_cen_dom_sf
IPR017923 TFIIS_N
IPR006289 TFSII
IPR001222 Znf_TFIIS
PfamiView protein in Pfam
PF08711 Med26, 1 hit
PF01096 TFIIS_C, 1 hit
PF07500 TFIIS_M, 1 hit
PIRSFiPIRSF006704 TF_IIS, 1 hit
SMARTiView protein in SMART
SM00510 TFS2M, 1 hit
SM00509 TFS2N, 1 hit
SM00440 ZnF_C2C2, 1 hit
SUPFAMiSSF46942 SSF46942, 1 hit
SSF47676 SSF47676, 1 hit
TIGRFAMsiTIGR01385 TFSII, 1 hit
PROSITEiView protein in PROSITE
PS51321 TFIIS_CENTRAL, 1 hit
PS51319 TFIIS_N, 1 hit
PS00466 ZF_TFIIS_1, 1 hit
PS51133 ZF_TFIIS_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: P23193-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEDEVVRFAK KMDKMVQKKN AAGALDLLKE LKNIPMTLEL LQSTRIGMSV
60 70 80 90 100
NAIRKQSTDE EVTSLAKSLI KSWKKLLDGP STEKDLDEKK KEPAITSQNS
110 120 130 140 150
PEAREESTSS GNVSNRKDET NARDTYVSSF PRAPSTSDSV RLKCREMLAA
160 170 180 190 200
ALRTGDDYIA IGADEEELGS QIEEAIYQEI RNTDMKYKNR VRSRISNLKD
210 220 230 240 250
AKNPNLRKNV LCGNIPPDLF ARMTAEEMAS DELKEMRKNL TKEAIREHQM
260 270 280 290 300
AKTGGTQTDL FTCGKCKKKN CTYTQVQTRS ADEPMTTFVV CNECGNRWKF

C
Length:301
Mass (Da):33,970
Last modified:November 1, 1995 - v2
Checksum:i8A685107A56D2DA1
GO
Isoform 2 (identifier: P23193-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     22-42: Missing.

Show »
Length:280
Mass (Da):31,664
Checksum:i31833641990136EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127V → L in AAA61138 (PubMed:1378807).Curated1
Sequence conflicti141R → Q in CAA51940 (PubMed:8112616).Curated1
Sequence conflicti205N → Y in CAA51940 (PubMed:8112616).Curated1
Sequence conflicti237R → W in CAA51940 (PubMed:8112616).Curated1
Sequence conflicti285M → V in CAA51940 (PubMed:8112616).Curated1
Sequence conflicti300F → I in AC100821 (Ref. 6) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00640922 – 42Missing in isoform 2. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X62585 mRNA Translation: CAA44470.1
X57198 mRNA Translation: CAA40484.1
M81601 mRNA Translation: AAA61138.1
X73534 Genomic DNA Translation: CAA51940.1
AK290454 mRNA Translation: BAF83143.1
CR542221 mRNA Translation: CAG47017.1
BT019995 mRNA Translation: AAV38798.1
AC100821 Genomic DNA No translation available.
BC072460 mRNA Translation: AAH72460.1
CCDSiCCDS47857.1 [P23193-2]
CCDS47858.1 [P23193-1]
PIRiS17361
S26831
S34159
RefSeqiNP_006747.1, NM_006756.3 [P23193-1]
NP_958845.1, NM_201437.2 [P23193-2]
UniGeneiHs.491745

Genome annotation databases

EnsembliENST00000396401; ENSP00000395483; ENSG00000187735 [P23193-2]
ENST00000521604; ENSP00000428426; ENSG00000187735 [P23193-1]
GeneIDi6917
KEGGihsa:6917
UCSCiuc003xru.5 human [P23193-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

Similar proteinsi

Entry informationi

Entry nameiTCEA1_HUMAN
AccessioniPrimary (citable) accession number: P23193
Secondary accession number(s): A6NF25
, A8K339, Q15563, Q6FG87
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 192 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health