P23192 (MTM2_MORBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase MboII
Short name=M.MboII
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase MboII
DNA MTase MboIIA

Gene names

Name:

mboIIM

Organism

Moraxella bovis

Taxonomic identifier

476 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Moraxella › Moraxella

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this enzyme.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

Modification methylase MboII

PRO_0000087967

Secondary structure

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260

Helix Strand Turn

Details...

Beta strand

4 – 9

6

Helix

12 – 18

7

Beta strand

24 – 29

6

Helix

39 – 41

3

Helix

46 – 63

18

Beta strand

64 – 74

11

Helix

76 – 88

13

Beta strand

92 – 99

8

Beta strand

109 – 111

3

Beta strand

117 – 125

9

Helix

132 – 134

3

Helix

141 – 151

11

Beta strand

170 – 173

4

Helix

199 – 209

11

Beta strand

215 – 220

6

Helix

225 – 232

8

Beta strand

236 – 242

7

Helix

244 – 255

12

Sequences

	Sequence		Length	Mass (Da)
	P23192 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: E53354DCA12DBE7C	FASTA	260	30,077
	10 20 30 40 50 60 MLEINKIHQM NCFDFLDQVE NKSVQLAVID PPYNLSKADW DSFDSHNEFL PFTYRWIDKV 70 80 90 100 110 120 LDKLDKDGSL YIFNTPFNCA FICQYLVSKG MIFQNWITWD KRDGMGSAKR GFSTGQETIL 130 140 150 160 170 180 FFSKSKNHTF NYDEVRVPYE STDRIKHASE KGILKNGKRW FPNPNGRLCG EVWHFSSQRH 190 200 210 220 230 240 KEKVNGKTVK LTHITPKPRD LIERIIRASS NPNDLVLDCF MGSGTTAIVA KKLGRNFIGC 250 260 DMNAEYVNQA NFVLNQLEIN

References

[1]	"Cloning and characterization of the MboII restriction-modification system." Bocklage H., Heeger K., Mueller-Hill B. Nucleic Acids Res. 19:1007-1013(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.
[2]	"Crystal structure of MboIIA methyltransferase." Osipiuk J., Walsh M.A., Joachimiak A. Nucleic Acids Res. 31:5440-5448(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X56977 Genomic DNA. Translation: CAA40297.1.

PIR

S26835.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1G60	X-ray	1.74	A/B	1-260	[»]

ProteinModelPortal

P23192.

SMR

P23192. Positions 1-256.

ModBase

Search...

Protein family/group databases

REBASE

3441. M1.MboII.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
[Graphical view]

Pfam

PF01555. N6_N4_Mtase. 1 hit.
[Graphical view]

PRINTS

PR00508. S21N4MTFRASE.

PROSITE

PS00092. N6_MTASE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P23192.

Entry information

Entry name

MTM2_MORBO

Accession

Primary (citable) accession number: P23192

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program