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P23192 (MTM2_MORBO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Modification methylase MboII

Short name=M.MboII
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase MboII
DNA MTase MboIIA
Gene names
Name:mboIIM
OrganismMoraxella bovis
Taxonomic identifier476 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GAAGA, causes specific methylation on A-5, and protects the DNA from cleavage by the MboII endonuclease. It is not known if the cytosine of the complementary sequence TCTTC is also methylated by this enzyme.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 260260Modification methylase MboII
PRO_0000087967

Secondary structure

.................................... 260
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23192 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: E53354DCA12DBE7C

FASTA26030,077
        10         20         30         40         50         60 
MLEINKIHQM NCFDFLDQVE NKSVQLAVID PPYNLSKADW DSFDSHNEFL PFTYRWIDKV 

        70         80         90        100        110        120 
LDKLDKDGSL YIFNTPFNCA FICQYLVSKG MIFQNWITWD KRDGMGSAKR GFSTGQETIL 

       130        140        150        160        170        180 
FFSKSKNHTF NYDEVRVPYE STDRIKHASE KGILKNGKRW FPNPNGRLCG EVWHFSSQRH 

       190        200        210        220        230        240 
KEKVNGKTVK LTHITPKPRD LIERIIRASS NPNDLVLDCF MGSGTTAIVA KKLGRNFIGC 

       250        260 
DMNAEYVNQA NFVLNQLEIN 

« Hide

References

[1]"Cloning and characterization of the MboII restriction-modification system."
Bocklage H., Heeger K., Mueller-Hill B.
Nucleic Acids Res. 19:1007-1013(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.
[2]"Crystal structure of MboIIA methyltransferase."
Osipiuk J., Walsh M.A., Joachimiak A.
Nucleic Acids Res. 31:5440-5448(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.74 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56977 Genomic DNA. Translation: CAA40297.1.
PIRS26835.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1G60X-ray1.74A/B1-260[»]
ProteinModelPortalP23192.
SMRP23192. Positions 1-256.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3441. M1.MboII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP23192.

Entry information

Entry nameMTM2_MORBO
AccessionPrimary (citable) accession number: P23192
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references