Reviewed, UniProtKB/Swiss-Prot P23191 (T2M2_MORBO)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme MboII
      Short name=R.MboII
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme MboII
    Type IIS restriction enzyme MboII
    Endonuclease MboII

Gene names

Name:

mboIIR

Organism

Moraxella bovis

Taxonomic identifier

476 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Moraxella

Protein attributes

Sequence length	416 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 416	416	Type-2 restriction enzyme MboII		PRO_0000077331

Sequences

	Sequence		Length	Mass (Da)
	P23191-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: C5A0008775B635CF	FASTA	416	48,617
	10 20 30 40 50 60 MKNYVSNINL GNSSLKFIDE RLQSENYRGI HLSQHNRYDL PKLIDILTLL NKHAPNQSLM 70 80 90 100 110 120 QIRTTDISKR PQNIPEEQSY AEFCNEAKSL TNIGTQDAMR KNLFVDFARM GLINRYNDKK 130 140 150 160 170 180 VLTDPFKRGV TKYVALSDMG VKLIDPKLDI LSKNLIFSKS LNKLLTGFVE DVLSLLTNSD 190 200 210 220 230 240 LKEISFDEFM LFVSAMNCNF NFSISTEQCE SLIKEYRLLS RVQKNAVIDT LKSELIPDNF 250 260 270 280 290 300 NGDKKDKRDY HNWANENQQI WTLFENIPFF IMEKDSRKLI LITSDVDLSK YSKSKMKRSQ 310 320 330 340 350 360 QAKNDYFKHH KVNKIKGYEL DHIIPLLEAE SVDEYRYLDN WLNLLYIDGK THAIKSQSGS 370 380 390 400 410 KYYIFTFDDN DYNQIYFLDT QGDKLSINND DTALFDKNKV PKIYEYNQNF INAKTS

References

[1]

"Cloning and characterization of the MboII restriction-modification system." Bocklage H., Heeger K., Mueller-Hill B. Nucleic Acids Res. 19:1007-1013(1991) [PubMed: 2020540] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5; 7-12 AND 15-19. Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X56977 Genomic DNA. Translation: CAA40298.1.
PIR	S26836.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.21.4. 96192.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2M2_MORBO

Accession

Primary (citable) accession number: P23191

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	July 7, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)