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P23191

- T2M2_MORBO

UniProt

P23191 - T2M2_MORBO

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Protein

Type-2 restriction enzyme MboII

Gene

mboIIR

Organism
Moraxella bovis
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

Magnesium.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi1205. MboII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MboII (EC:3.1.21.4)
Short name:
R.MboII
Alternative name(s):
Endonuclease MboII
Type II restriction enzyme MboII
Type IIS restriction enzyme MboII
Gene namesi
Name:mboIIR
OrganismiMoraxella bovis
Taxonomic identifieri476 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Type-2 restriction enzyme MboIIPRO_0000077331Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP23191.
ModBaseiSearch...
MobiDBiSearch...

Sequencei

Sequence statusi: Complete.

P23191-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MKNYVSNINL GNSSLKFIDE RLQSENYRGI HLSQHNRYDL PKLIDILTLL
60 70 80 90 100
NKHAPNQSLM QIRTTDISKR PQNIPEEQSY AEFCNEAKSL TNIGTQDAMR
110 120 130 140 150
KNLFVDFARM GLINRYNDKK VLTDPFKRGV TKYVALSDMG VKLIDPKLDI
160 170 180 190 200
LSKNLIFSKS LNKLLTGFVE DVLSLLTNSD LKEISFDEFM LFVSAMNCNF
210 220 230 240 250
NFSISTEQCE SLIKEYRLLS RVQKNAVIDT LKSELIPDNF NGDKKDKRDY
260 270 280 290 300
HNWANENQQI WTLFENIPFF IMEKDSRKLI LITSDVDLSK YSKSKMKRSQ
310 320 330 340 350
QAKNDYFKHH KVNKIKGYEL DHIIPLLEAE SVDEYRYLDN WLNLLYIDGK
360 370 380 390 400
THAIKSQSGS KYYIFTFDDN DYNQIYFLDT QGDKLSINND DTALFDKNKV
410
PKIYEYNQNF INAKTS
Length:416
Mass (Da):48,617
Last modified:November 1, 1991 - v1
Checksum:iC5A0008775B635CF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56977 Genomic DNA. Translation: CAA40298.1.
PIRiS26836.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56977 Genomic DNA. Translation: CAA40298.1 .
PIRi S26836.

3D structure databases

ProteinModelPortali P23191.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 1205. MboII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Cloning and characterization of the MboII restriction-modification system."
    Bocklage H., Heeger K., Mueller-Hill B.
    Nucleic Acids Res. 19:1007-1013(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5; 7-12 AND 15-19.
    Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Entry informationi

Entry nameiT2M2_MORBO
AccessioniPrimary (citable) accession number: P23191
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3