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Protein

Type-2 restriction enzyme MboII

Gene

mboIIR

Organism
Moraxella bovis
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium

Protein family/group databases

REBASEi1205 MboII

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme MboII (EC:3.1.21.4)
Short name:
R.MboII
Alternative name(s):
Endonuclease MboII
Type II restriction enzyme MboII
Type IIS restriction enzyme MboII
Gene namesi
Name:mboIIR
OrganismiMoraxella bovis
Taxonomic identifieri476 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773311 – 416Type-2 restriction enzyme MboIIAdd BLAST416

Proteomic databases

PRIDEiP23191

Structurei

3D structure databases

ProteinModelPortaliP23191
ModBaseiSearch...
MobiDBiSearch...

Sequencei

Sequence statusi: Complete.

P23191-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNYVSNINL GNSSLKFIDE RLQSENYRGI HLSQHNRYDL PKLIDILTLL
60 70 80 90 100
NKHAPNQSLM QIRTTDISKR PQNIPEEQSY AEFCNEAKSL TNIGTQDAMR
110 120 130 140 150
KNLFVDFARM GLINRYNDKK VLTDPFKRGV TKYVALSDMG VKLIDPKLDI
160 170 180 190 200
LSKNLIFSKS LNKLLTGFVE DVLSLLTNSD LKEISFDEFM LFVSAMNCNF
210 220 230 240 250
NFSISTEQCE SLIKEYRLLS RVQKNAVIDT LKSELIPDNF NGDKKDKRDY
260 270 280 290 300
HNWANENQQI WTLFENIPFF IMEKDSRKLI LITSDVDLSK YSKSKMKRSQ
310 320 330 340 350
QAKNDYFKHH KVNKIKGYEL DHIIPLLEAE SVDEYRYLDN WLNLLYIDGK
360 370 380 390 400
THAIKSQSGS KYYIFTFDDN DYNQIYFLDT QGDKLSINND DTALFDKNKV
410
PKIYEYNQNF INAKTS
Length:416
Mass (Da):48,617
Last modified:November 1, 1991 - v1
Checksum:iC5A0008775B635CF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56977 Genomic DNA Translation: CAA40298.1
PIRiS26836

Similar proteinsi

Entry informationi

Entry nameiT2M2_MORBO
AccessioniPrimary (citable) accession number: P23191
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 23, 2018
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

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