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P23191

- T2M2_MORBO

UniProt

P23191 - T2M2_MORBO

Protein

Type-2 restriction enzyme MboII

Gene

mboIIR

Organism
Moraxella bovis
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (16 Apr 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi1205. MboII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme MboII (EC:3.1.21.4)
    Short name:
    R.MboII
    Alternative name(s):
    Endonuclease MboII
    Type II restriction enzyme MboII
    Type IIS restriction enzyme MboII
    Gene namesi
    Name:mboIIR
    OrganismiMoraxella bovis
    Taxonomic identifieri476 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 416416Type-2 restriction enzyme MboIIPRO_0000077331Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP23191.
    ModBaseiSearch...
    MobiDBiSearch...

    Sequencei

    Sequence statusi: Complete.

    P23191-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNYVSNINL GNSSLKFIDE RLQSENYRGI HLSQHNRYDL PKLIDILTLL    50
    NKHAPNQSLM QIRTTDISKR PQNIPEEQSY AEFCNEAKSL TNIGTQDAMR 100
    KNLFVDFARM GLINRYNDKK VLTDPFKRGV TKYVALSDMG VKLIDPKLDI 150
    LSKNLIFSKS LNKLLTGFVE DVLSLLTNSD LKEISFDEFM LFVSAMNCNF 200
    NFSISTEQCE SLIKEYRLLS RVQKNAVIDT LKSELIPDNF NGDKKDKRDY 250
    HNWANENQQI WTLFENIPFF IMEKDSRKLI LITSDVDLSK YSKSKMKRSQ 300
    QAKNDYFKHH KVNKIKGYEL DHIIPLLEAE SVDEYRYLDN WLNLLYIDGK 350
    THAIKSQSGS KYYIFTFDDN DYNQIYFLDT QGDKLSINND DTALFDKNKV 400
    PKIYEYNQNF INAKTS 416
    Length:416
    Mass (Da):48,617
    Last modified:November 1, 1991 - v1
    Checksum:iC5A0008775B635CF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56977 Genomic DNA. Translation: CAA40298.1.
    PIRiS26836.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56977 Genomic DNA. Translation: CAA40298.1 .
    PIRi S26836.

    3D structure databases

    ProteinModelPortali P23191.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 1205. MboII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the MboII restriction-modification system."
      Bocklage H., Heeger K., Mueller-Hill B.
      Nucleic Acids Res. 19:1007-1013(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5; 7-12 AND 15-19.
      Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

    Entry informationi

    Entry nameiT2M2_MORBO
    AccessioniPrimary (citable) accession number: P23191
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: April 16, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3