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P23191 (T2M2_MORBO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme MboII

Short name=R.MboII
EC=3.1.21.4
Alternative name(s):
Endonuclease MboII
Type II restriction enzyme MboII
Type IIS restriction enzyme MboII
Gene names
Name:mboIIR
OrganismMoraxella bovis
Taxonomic identifier476 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaeMoraxella

Protein attributes

Sequence length416 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T-1, leaving a single 3' protruding nucleotide.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 416416Type-2 restriction enzyme MboII
PRO_0000077331

Sequences

Sequence LengthMass (Da)Tools
P23191 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: C5A0008775B635CF

FASTA41648,617
        10         20         30         40         50         60 
MKNYVSNINL GNSSLKFIDE RLQSENYRGI HLSQHNRYDL PKLIDILTLL NKHAPNQSLM 

        70         80         90        100        110        120 
QIRTTDISKR PQNIPEEQSY AEFCNEAKSL TNIGTQDAMR KNLFVDFARM GLINRYNDKK 

       130        140        150        160        170        180 
VLTDPFKRGV TKYVALSDMG VKLIDPKLDI LSKNLIFSKS LNKLLTGFVE DVLSLLTNSD 

       190        200        210        220        230        240 
LKEISFDEFM LFVSAMNCNF NFSISTEQCE SLIKEYRLLS RVQKNAVIDT LKSELIPDNF 

       250        260        270        280        290        300 
NGDKKDKRDY HNWANENQQI WTLFENIPFF IMEKDSRKLI LITSDVDLSK YSKSKMKRSQ 

       310        320        330        340        350        360 
QAKNDYFKHH KVNKIKGYEL DHIIPLLEAE SVDEYRYLDN WLNLLYIDGK THAIKSQSGS 

       370        380        390        400        410 
KYYIFTFDDN DYNQIYFLDT QGDKLSINND DTALFDKNKV PKIYEYNQNF INAKTS 

« Hide

References

[1]"Cloning and characterization of the MboII restriction-modification system."
Bocklage H., Heeger K., Mueller-Hill B.
Nucleic Acids Res. 19:1007-1013(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-5; 7-12 AND 15-19.
Strain: ATCC 10900 / DSM 6328 / LMG 986 / NCTC 11013.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56977 Genomic DNA. Translation: CAA40298.1.
PIRS26836.

3D structure databases

ProteinModelPortalP23191.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE1205. MboII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2M2_MORBO
AccessionPrimary (citable) accession number: P23191
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries