Reviewed, UniProtKB/Swiss-Prot P23189 (GSHR_PSEAE)

Names and origin

Protein names

Recommended name:
    Glutathione reductase
      Short name=GRase
      Short name=GR
    EC=1.8.1.7

Gene names

Name:	gor
Ordered Locus Names:	PA2025

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Maintains high levels of reduced glutathione in the cytosol.
Catalytic activity	2 glutathione + NADP+ = glutathione disulfide + NADPH.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Miscellaneous	The active site is a redox-active disulfide bond.
Sequence similarities	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	Redox-active center
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro glutathione-disulfide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 451	451	Glutathione reductase		PRO_0000067977
Regions
	Nucleotide binding	34 – 42	9	FAD By similarity
Sites
	Active site	436	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	42 ↔ 47		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23189-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 2924599996DB98EA	FASTA	451	49,237
	10 20 30 40 50 60 MSFDFDLFVI GAGSGGVRAA RFAAGFGARV AVAESRYLGG TCVNVGCVPK KLLVYGAHFS 70 80 90 100 110 120 EDFEQARAYG WSAGEAQFDW ATLIGNKNRE IQRLNGIYRN LLVNSGVTLL EGHARLLDAH 130 140 150 160 170 180 SVEVDGQRFS AKHILVATGG WPQVPDIPGK EHAITSNEAF FLERLPRRVL VVGGGYIAVE 190 200 210 220 230 240 FASIFNGLGA ETTLLYRRDL FLRGFDRSVR EHLRDELGKK GLDLQFNSDI ARIDKQADGS 250 260 270 280 290 300 LAATLKDGRV LEADCVFYAT GRRPMLDDLG LENTAVKLTD KGFIAVDEHY QTSEPSILAL 310 320 330 340 350 360 GDVIGRVQLT PVALAEGMAV ARRLFKPEEY RPVDYKLIPT AVFSLPNIGT VGLTEEEALS 370 380 390 400 410 420 AGHKVKIFES RFRPMKLTLT DDQEKTLMKL VVDAHDDRVL GCHMVGAEAG EILQGIAVAM 430 440 450 KAGATKQAFD ETIGIHPTAA EEFVTLRTPT R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of the gor gene encoding glutathione reductase from Pseudomonas aeruginosa: determinants of substrate specificity among pyridine nucleotide-disulphide oxidoreductases." Perry A.C.F., Ni Bhriain N., Brown N.L., Rouch D.A. Mol. Microbiol. 5:163-171(1991) [PubMed: 1849605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: PAO8.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54201 Genomic DNA. Translation: CAA38122.1. AE004091 Genomic DNA. Translation: AAG05413.1.
PIR	S15236.
RefSeq	NP_250715.1.
3D structure databases
SMR	P23189. Positions 3-450.
ModBase	Search...
Genome annotation databases
GeneID	878570.
GenomeReviews	Gene locus PA2025 in contig AE004091_GR.
KEGG	pae:PA2025.
Organism-specific databases
PseudoCAP	PA2025.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG515043.
OMA	SEDFDHA.
Enzyme and pathway databases
BioCyc	PAER208964:PA2025-MONOMER.
BRENDA	1.8.1.7. 354.
Family and domain databases
InterPro	IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR012999. Pyr_OxRdtase_I_AS. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Gene3D	G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit.
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00945. HGRDTASE.
PROSITE	PS00076. PYRIDINE_REDOX_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHR_PSEAE

Accession

Primary (citable) accession number: P23189

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	February 9, 2010
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)