Reviewed, UniProtKB/Swiss-Prot P23179 (SPO11_YEAST)

Names and origin

Protein names

Recommended name:
    Meiosis-specific protein SPO11
Alternative name(s):
    Sporulation-specific protein 11

Gene names

Name:	SPO11
Ordered Locus Names:	YHL022C

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The action of SPO11 is important in setting off a regulatory chain of events encompassing 5' to 3' resection. When there are no SPO11-DSBs, resection of a site specific VDE-DSB takes place but it is faster than in wild-type meiosis and increases the risk of uncovering flanking homology. Ref.4 Ref.6
Subcellular location	Nucleus.
Developmental stage	Meiosis-specific.
Sequence similarities	Belongs to the TOP6A family.

Ontologies

Keywords
Biological process	Meiosis Sporulation
Cellular component	Nucleus
Ligand	DNA-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	meiotic DNA double-strand break formation Inferred from genetic interaction. Source: SGD sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nuclear chromosome Inferred from physical interaction. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW endodeoxyribonuclease activity, producing 3'-phosphomonoesters Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Meiosis-specific protein SPO11		PRO_0000145473
Sites
	Active site	135	1	For DNA cleavage activity Probable
Experimental info
	Mutagenesis	135	1	Y → F: Loss of activity. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P23179-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: FDDB079411DB3C71	FASTA	398	45,413
	10 20 30 40 50 60 MALEGLRKKY KTRQELVKAL TPKRRSIHLN SNGHSNGTPC SNADVLAHIK HFLSLAANSL 70 80 90 100 110 120 EQHQQPISIV FQNKKKKGDT SSPDIHTTLD FPLNGPHLCT HQFKLKRCAI LLNLLKVVME 130 140 150 160 170 180 KLPLGKNTTV RDIFYSNVEL FQRQANVVQW LDVIRFNFKL SPRKSLNIIP AQKGLVYSPF 190 200 210 220 230 240 PIDIYDNILT CENEPKMQKQ TIFPGKPCLI PFFQDDAVIK LGTTSMCNIV IVEKEAVFTK 250 260 270 280 290 300 LVNNYHKLST NTMLITGKGF PDFLTRLFLK KLEQYCSKLI SDCSIFTDAD PYGISIALNY 310 320 330 340 350 360 THSNERNAYI CTMANYKGIR ITQVLAQNNE VHNKSIQLLS LNQRDYSLAK NLIASLTANS 370 380 390 WDIATSPLKN VIIECQREIF FQKKAEMNEI DARIFEYK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene." Atcheson C.L., Didomenico B., Frackman S., Esposito R.E., Elder R.T. Proc. Natl. Acad. Sci. U.S.A. 84:8035-8039(1987) [PubMed: 3317399] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome VIII." Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z., Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T., Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P. , Louis E.J., Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L., St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P., Waterston R., Wilson R., Vaudin M. Science 265:2077-2082(1994) [PubMed: 8091229] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"An atypical topoisomerase II from Archaea with implications for meiotic recombination." Bergerat A., de Massy B., Gadelle D., Varoutas P.-C., Nicolas A., Forterre P. Nature 386:414-417(1997) [PubMed: 9121560] [Abstract] Cited for: POSSIBLE FUNCTION, MUTAGENESIS OF TYR-135.
[5]	"Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae." Burns N., Grimwade B., Ross-Macdonald P.B., Choi E.Y., Finberg K., Roeder G.S., Snyder M. Genes Dev. 8:1087-1105(1994) [PubMed: 7926789] [Abstract] Cited for: SUBCELLULAR LOCATION.
[6]	"Wild-type levels of Spo11-induced DSBs are required for normal single-strand resection during meiosis." Neale M.J., Ramachandran M., Trelles-Sticken E., Scherthan H., Goldman A.S. Mol. Cell 9:835-846(2002) [PubMed: 11983174] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	J02987 Genomic DNA. Translation: AAA65532.1. U11582 Genomic DNA. Translation: AAB65075.1. AY557836 Genomic DNA. Translation: AAS56162.1.
PIR	BWBY11. S05854.
RefSeq	NP_011841.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1652N.
IntAct	P23179. 2 interactions.
STRING	P23179.
Genome annotation databases
Ensembl	YHL022C; YHL022C; YHL022C; Saccharomyces cerevisiae. [Genome view]
GeneID	856364.
KEGG	sce:YHL022C.
NMPDR	fig|4932.3.peg.2979.
Organism-specific databases
CYGD	YHL022c.
SGD	S000001014. SPO11.
Phylogenomic databases
OMA	NEIDARI
OrthoDB	EOG9Q5B6B
Gene expression databases
ArrayExpress	P23179.
Genevestigator	P23179.
GermOnline	YHL022C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002815. Spo11/TopoVI_A. IPR013049. Spo11/TopoVI_A_N. [Graphical view]
PANTHER	PTHR10848. Spo11/TopoVI_A. 1 hit.
Pfam	PF04406. TP6A_N. 1 hit. [Graphical view]
PRINTS	PR01550. TOP6AFAMILY.
ProtoNet	Search...
Other Resources
NextBio	981828.

Entry information

Entry name

SPO11_YEAST

Accession

Primary (citable) accession number: P23179

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	November 24, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)