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Protein

Meiosis-specific protein SPO11

Gene

SPO11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The action of SPO11 is important in setting off a regulatory chain of events encompassing 5' to 3' resection. When there are no SPO11-DSBs, resection of a site specific VDE-DSB takes place but it is faster than in wild-type meiosis and increases the risk of uncovering flanking homology.2 Publications

Catalytic activityi

ATP-dependent breakage, passage and rejoining of double-stranded DNA.Curated

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei135Nucleophile1 Publication1
Metal bindingi233MagnesiumBy similarity1
Metal bindingi288MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: InterPro
  • chromatin binding Source: SGD
  • DNA binding Source: GO_Central
  • DNA topoisomerase type II (ATP-hydrolyzing) activity Source: GO_Central
  • endodeoxyribonuclease activity, producing 3'-phosphomonoesters Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Hydrolase, Isomerase
Biological processMeiosis, Sporulation
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31042-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Meiosis-specific protein SPO11 (EC:5.99.1.3Curated)
Alternative name(s):
Sporulation-specific protein 11
Gene namesi
Name:SPO11
Ordered Locus Names:YHL022C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHL022C
SGDiS000001014 SPO11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi135Y → F: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001454731 – 398Meiosis-specific protein SPO11Add BLAST398

Proteomic databases

PaxDbiP23179
PRIDEiP23179

Expressioni

Developmental stagei

Meiosis-specific.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi36401, 110 interactors
DIPiDIP-1652N
IntActiP23179, 5 interactors
MINTiP23179
STRINGi4932.YHL022C

Structurei

3D structure databases

ProteinModelPortaliP23179
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

HOGENOMiHOG000000868
InParanoidiP23179
KOiK10878
OMAiTVRDIYY
OrthoDBiEOG092C5FRF

Family and domain databases

CDDicd00223 TOPRIM_TopoIIB_SPO, 1 hit
InterProiView protein in InterPro
IPR002815 Spo11/TopoVI_A
IPR013049 Spo11/TopoVI_A_N
IPR036078 Spo11/TopoVI_A_sf
IPR034136 TOPRIM_Topo6A/Spo11
PANTHERiPTHR10848 PTHR10848, 1 hit
PfamiView protein in Pfam
PF04406 TP6A_N, 1 hit
PRINTSiPR01550 TOP6AFAMILY
SUPFAMiSSF56726 SSF56726, 1 hit

Sequencei

Sequence statusi: Complete.

P23179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALEGLRKKY KTRQELVKAL TPKRRSIHLN SNGHSNGTPC SNADVLAHIK
60 70 80 90 100
HFLSLAANSL EQHQQPISIV FQNKKKKGDT SSPDIHTTLD FPLNGPHLCT
110 120 130 140 150
HQFKLKRCAI LLNLLKVVME KLPLGKNTTV RDIFYSNVEL FQRQANVVQW
160 170 180 190 200
LDVIRFNFKL SPRKSLNIIP AQKGLVYSPF PIDIYDNILT CENEPKMQKQ
210 220 230 240 250
TIFPGKPCLI PFFQDDAVIK LGTTSMCNIV IVEKEAVFTK LVNNYHKLST
260 270 280 290 300
NTMLITGKGF PDFLTRLFLK KLEQYCSKLI SDCSIFTDAD PYGISIALNY
310 320 330 340 350
THSNERNAYI CTMANYKGIR ITQVLAQNNE VHNKSIQLLS LNQRDYSLAK
360 370 380 390
NLIASLTANS WDIATSPLKN VIIECQREIF FQKKAEMNEI DARIFEYK
Length:398
Mass (Da):45,413
Last modified:November 1, 1991 - v1
Checksum:iFDDB079411DB3C71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02987 Genomic DNA Translation: AAA65532.1
U11582 Genomic DNA Translation: AAB65075.1
AY557836 Genomic DNA Translation: AAS56162.1
BK006934 Genomic DNA Translation: DAA06663.1
PIRiS05854 BWBY11
RefSeqiNP_011841.1, NM_001179102.1

Genome annotation databases

EnsemblFungiiYHL022C; YHL022C; YHL022C
GeneIDi856364
KEGGisce:YHL022C

Similar proteinsi

Entry informationi

Entry nameiSPO11_YEAST
AccessioniPrimary (citable) accession number: P23179
Secondary accession number(s): D3DKP3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: March 28, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health