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Protein

Meiosis-specific protein SPO11

Gene

SPO11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for meiotic recombination. Mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination. The action of SPO11 is important in setting off a regulatory chain of events encompassing 5' to 3' resection. When there are no SPO11-DSBs, resection of a site specific VDE-DSB takes place but it is faster than in wild-type meiosis and increases the risk of uncovering flanking homology.1 Publication

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei135 – 1351Nucleophile1 Publication
Metal bindingi233 – 2331MagnesiumBy similarity
Metal bindingi288 – 2881MagnesiumBy similarity

GO - Molecular functioni

  • ATP binding Source: InterPro
  • chromatin binding Source: SGD
  • DNA binding Source: UniProtKB-KW
  • endodeoxyribonuclease activity, producing 3'-phosphomonoesters Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Meiosis, Sporulation

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31042-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Meiosis-specific protein SPO11
Alternative name(s):
Sporulation-specific protein 11
Gene namesi
Name:SPO11
Ordered Locus Names:YHL022C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VIII

Organism-specific databases

EuPathDBiFungiDB:YHL022C.
SGDiS000001014. SPO11.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome Source: SGD
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi135 – 1351Y → F: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 398398Meiosis-specific protein SPO11PRO_0000145473Add
BLAST

Proteomic databases

PRIDEiP23179.

Expressioni

Developmental stagei

Meiosis-specific.

Interactioni

Protein-protein interaction databases

BioGridi36401. 71 interactions.
DIPiDIP-1652N.
IntActiP23179. 2 interactions.
MINTiMINT-398270.

Structurei

3D structure databases

ProteinModelPortaliP23179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TOP6A family.Curated

Phylogenomic databases

HOGENOMiHOG000000868.
InParanoidiP23179.
KOiK10878.
OMAiSKANERQ.
OrthoDBiEOG7P2Z3N.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.

Sequencei

Sequence statusi: Complete.

P23179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALEGLRKKY KTRQELVKAL TPKRRSIHLN SNGHSNGTPC SNADVLAHIK
60 70 80 90 100
HFLSLAANSL EQHQQPISIV FQNKKKKGDT SSPDIHTTLD FPLNGPHLCT
110 120 130 140 150
HQFKLKRCAI LLNLLKVVME KLPLGKNTTV RDIFYSNVEL FQRQANVVQW
160 170 180 190 200
LDVIRFNFKL SPRKSLNIIP AQKGLVYSPF PIDIYDNILT CENEPKMQKQ
210 220 230 240 250
TIFPGKPCLI PFFQDDAVIK LGTTSMCNIV IVEKEAVFTK LVNNYHKLST
260 270 280 290 300
NTMLITGKGF PDFLTRLFLK KLEQYCSKLI SDCSIFTDAD PYGISIALNY
310 320 330 340 350
THSNERNAYI CTMANYKGIR ITQVLAQNNE VHNKSIQLLS LNQRDYSLAK
360 370 380 390
NLIASLTANS WDIATSPLKN VIIECQREIF FQKKAEMNEI DARIFEYK
Length:398
Mass (Da):45,413
Last modified:November 1, 1991 - v1
Checksum:iFDDB079411DB3C71
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02987 Genomic DNA. Translation: AAA65532.1.
U11582 Genomic DNA. Translation: AAB65075.1.
AY557836 Genomic DNA. Translation: AAS56162.1.
BK006934 Genomic DNA. Translation: DAA06663.1.
PIRiS05854. BWBY11.
RefSeqiNP_011841.1. NM_001179102.1.

Genome annotation databases

EnsemblFungiiYHL022C; YHL022C; YHL022C.
GeneIDi856364.
KEGGisce:YHL022C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02987 Genomic DNA. Translation: AAA65532.1.
U11582 Genomic DNA. Translation: AAB65075.1.
AY557836 Genomic DNA. Translation: AAS56162.1.
BK006934 Genomic DNA. Translation: DAA06663.1.
PIRiS05854. BWBY11.
RefSeqiNP_011841.1. NM_001179102.1.

3D structure databases

ProteinModelPortaliP23179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi36401. 71 interactions.
DIPiDIP-1652N.
IntActiP23179. 2 interactions.
MINTiMINT-398270.

Proteomic databases

PRIDEiP23179.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYHL022C; YHL022C; YHL022C.
GeneIDi856364.
KEGGisce:YHL022C.

Organism-specific databases

EuPathDBiFungiDB:YHL022C.
SGDiS000001014. SPO11.

Phylogenomic databases

HOGENOMiHOG000000868.
InParanoidiP23179.
KOiK10878.
OMAiSKANERQ.
OrthoDBiEOG7P2Z3N.

Enzyme and pathway databases

BioCyciYEAST:G3O-31042-MONOMER.

Miscellaneous databases

NextBioi981828.
PROiP23179.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR002815. Spo11/TopoVI_A.
IPR013049. Spo11/TopoVI_A_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10848. PTHR10848. 1 hit.
PfamiPF04406. TP6A_N. 1 hit.
[Graphical view]
PRINTSiPR01550. TOP6AFAMILY.
SUPFAMiSSF56726. SSF56726. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene."
    Atcheson C.L., Didomenico B., Frackman S., Esposito R.E., Elder R.T.
    Proc. Natl. Acad. Sci. U.S.A. 84:8035-8039(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "An atypical topoisomerase II from Archaea with implications for meiotic recombination."
    Bergerat A., de Massy B., Gadelle D., Varoutas P.-C., Nicolas A., Forterre P.
    Nature 386:414-417(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION, MUTAGENESIS OF TYR-135.
  6. "Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae."
    Burns N., Grimwade B., Ross-Macdonald P.B., Choi E.Y., Finberg K., Roeder G.S., Snyder M.
    Genes Dev. 8:1087-1105(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "Wild-type levels of Spo11-induced DSBs are required for normal single-strand resection during meiosis."
    Neale M.J., Ramachandran M., Trelles-Sticken E., Scherthan H., Goldman A.S.
    Mol. Cell 9:835-846(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSPO11_YEAST
AccessioniPrimary (citable) accession number: P23179
Secondary accession number(s): D3DKP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 11, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VIII
    Yeast (Saccharomyces cerevisiae) chromosome VIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.