P23175 (RASH_MSVNS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GTPase HRas

Alternative name(s):
Transforming protein p21/H-Ras

Gene names

Name:

H-RAS

Organism

Murine sarcoma virus NS.C58

Taxonomic identifier

11815 [NCBI]

Taxonomic lineage

Viruses › Retro-transcribing viruses › Retroviridae › Orthoretrovirinae › Gammaretrovirus › Mammalian virus group › Replication defective viruses › Moloney murine sarcoma virus

Virus host

Mus musculus (Mouse) [TaxID: 10090]

Protein attributes

Sequence length	189 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Enzyme regulation	Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
Subcellular location	Host cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the small GTPase superfamily. Ras family.

Ontologies

Keywords
Cellular component	Host cell membrane Host membrane Membrane
Disease	Oncogene
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Palmitate Prenylation
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	host cell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 186	186	GTPase HRas		PRO_0000030181
	Propeptide	187 – 189	3	Removed in mature form Probable		PRO_0000030182
Regions
	Nucleotide binding	10 – 17	8	GTP
	Nucleotide binding	57 – 61	5	GTP
	Nucleotide binding	116 – 119	4	GTP
	Motif	32 – 40	9	Effector region By similarity
Amino acid modifications
	Modified residue	186	1	Cysteine methyl ester; by host Probable
	Lipidation	181	1	S-palmitoyl cysteine; by host Potential
	Lipidation	184	1	S-palmitoyl cysteine; by host Potential
	Lipidation	186	1	S-farnesyl cysteine; by host Probable

Sequences

	Sequence		Length	Mass (Da)
	P23175 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 1B6272D68C479FC0	FASTA	189	21,397
	10 20 30 40 50 60 MTEYKLVVVG ARGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET CLLDILDTAG 70 80 90 100 110 120 QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI KRVKDSDDVP MVLVGNKCDL 130 140 150 160 170 180 AARTVESRQA QDLARSYGIP YIETSAKTRQ GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCVLS

References

[1]

"Biologic and molecular characterization of two newly isolated ras-containing murine leukemia viruses." Fredrickson T.N., O'Neill R.R., Rutledge R.A., Theodore T.S., Martin M.A., Ruscetti S.K., Austin J.B., Hartley J.W. J. Virol. 61:2109-2119(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M30733 Genomic RNA. Translation: AAA46574.1.
3D structure databases
ProteinModelPortal	P23175.
SMR	P23175. Positions 1-166.
ModBase	Search...
Proteomic databases
PRIDE	P23175.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. IPR020849. Small_GTPase_Ras. [Graphical view]
PANTHER	PTHR24070. PTHR24070. 1 hit.
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
SMART	SM00173. RAS. 1 hit. [Graphical view]
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51421. RAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RASH_MSVNS

Accession

Primary (citable) accession number: P23175

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program