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P23174 (MDR3_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multidrug resistance protein 3
EC=3.6.3.44
Alternative name(s):
P-glycoprotein 3
Gene names
Name:PGY3
Synonyms:PGP3
OrganismCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifier10029 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length1281 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency By similarity.

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Miscellaneous

PGP isoforms differ in their drug transport capabilities: PGP1 and PGP2 can mediate MDR, while PGP3 apparently cannot.

Sequence similarities

Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell membrane
Membrane
   DomainRepeat
Transmembrane
Transmembrane helix
   LigandATP-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMGlycoprotein
Phosphoprotein
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

xenobiotic-transporting ATPase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12811281Multidrug resistance protein 3
PRO_0000093341

Regions

Topological domain1 – 5050Cytoplasmic By similarity
Transmembrane51 – 7323Helical; By similarity
Topological domain74 – 11845Extracellular By similarity
Transmembrane119 – 13921Helical; By similarity
Topological domain140 – 18849Cytoplasmic By similarity
Transmembrane189 – 21022Helical; By similarity
Topological domain211 – 2177Extracellular By similarity
Transmembrane218 – 23821Helical; By similarity
Topological domain239 – 29658Cytoplasmic By similarity
Transmembrane297 – 31822Helical; By similarity
Topological domain319 – 33214Extracellular By similarity
Transmembrane333 – 35422Helical; By similarity
Topological domain355 – 713359Cytoplasmic By similarity
Transmembrane714 – 73421Helical; By similarity
Topological domain735 – 75723Extracellular By similarity
Transmembrane758 – 77821Helical; By similarity
Topological domain779 – 83355Cytoplasmic By similarity
Transmembrane834 – 85421Helical; By similarity
Topological domain8551Extracellular By similarity
Transmembrane856 – 87520Helical; By similarity
Topological domain876 – 93762Cytoplasmic By similarity
Transmembrane938 – 95821Helical; By similarity
Topological domain959 – 97416Extracellular By similarity
Transmembrane975 – 99622Helical; By similarity
Topological domain997 – 1281285Cytoplasmic By similarity
Domain57 – 359303ABC transmembrane type-1 1
Domain394 – 630237ABC transporter 1
Domain713 – 1001289ABC transmembrane type-1 2
Domain1036 – 1274239ABC transporter 2
Nucleotide binding429 – 4368ATP 1 Potential
Nucleotide binding1071 – 10788ATP 2 Potential

Amino acid modifications

Modified residue6721Phosphoserine By similarity
Glycosylation911N-linked (GlcNAc...) Potential
Glycosylation971N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P23174 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 2203EF61EBB29602

FASTA1,281140,866
        10         20         30         40         50         60 
MDLEAARNGT ARRPGTVEGD FELGSISNQG RNKKKKVNLI GPLTLFRYSD WQDKLFMLLG 

        70         80         90        100        110        120 
TIMAIAHGSG LPLMMIVFGE MTDKFVNNAG NFSLPVNFSL SMINPGRILE EEMTRYAYYY 

       130        140        150        160        170        180 
SGLGGGVLVA AYIQVSFWTL AAGRQIKKIR QNFFHAILRQ EMGWFDIKGT TELNTRLTDD 

       190        200        210        220        230        240 
ISKISEGIGD KVGMFFQAVA TFFAGFIVGF IRGWKLTLVI MAISPILGLS AAVWAKILST 

       250        260        270        280        290        300 
FSDKELAAYA KAGAVAEEAL GAIRTVIAFG GQNKELERYQ KHLENAKKIG IKKAISANIS 

       310        320        330        340        350        360 
MGIAFLLIYA SYALAFWYGS TLVISKEYTI GNAMTVFFSI LIGAFSVGQA APCIDAFANA 

       370        380        390        400        410        420 
RGAAYVIFDI IDNNPKIDSF SERGHKPDSI KGNLDFSDVH FSYPSRANIK ILKGLNLKVQ 

       430        440        450        460        470        480 
SGQTVALVGN SGCGKTTTLQ LLQRLYDPTE GTISIDGQDI RNFNVRYLRE IIGVVSQEPV 

       490        500        510        520        530        540 
LFSTTIAENI RYGRGNVTME EIKKAVKEAN AYEFIMKLPQ KFDTLVGERG AQLSGGQKQR 

       550        560        570        580        590        600 
IAIARALVRN PKILLLDEAT SALDTESEAE VQAALDKARE GRTTIVIAHR LSTVRNADVI 

       610        620        630        640        650        660 
AGFEDGVIVE QGSHSELMQK EGVYFKLVNM QTSGSQILSQ EFEVELSEEK AADGMTPNGW 

       670        680        690        700        710        720 
KSHIFRNSTK KSLKSSRAHH HRLDVDADEL DANVPPVSFL KVLKLNKTEW PYFVVGTVCA 

       730        740        750        760        770        780 
IVNGALQPAI SIILSEMIAI FGPGDDAVKQ QKCNLFSLVF LGLGVLSFFT FFLQGFTFGK 

       790        800        810        820        830        840 
AGEILTTRLR SMAFKAMLRQ DMSWFDDYKN STGALSTRLA TDRAQVQGAT GTRLALIAQN 

       850        860        870        880        890        900 
TANLGTGIII SFIYGWQLTL LLLSVVPFIA VSGIVEMKML AGNAKRDKKA LEAAGKIATE 

       910        920        930        940        950        960 
AIENIRTVVS LTQERKFESM YVEKLHEPYR NSVQMAHIYG ITFSISQAFM YFSYAGCFRF 

       970        980        990       1000       1010       1020 
GAYLIVNGHM RFRDVILVFS AIVFGAVALG HASSFAPDYA KAKLSAAHLF SLFERQPLID 

      1030       1040       1050       1060       1070       1080 
SYSGEGLWPD KFEGSVTFNE VVFNYPTRAN MPVLQGLSLE VKKGQTLALV GSSGCGKSTV 

      1090       1100       1110       1120       1130       1140 
VQLLERFYDP MAGTVLLDGQ EAKKLNIQWL RAQLGIVSQE PVLFDCSIAE NIAYGDNSRV 

      1150       1160       1170       1180       1190       1200 
VSQDEIVRAA KAANIHPFIE TLPQKYKTRV GDKGTQLSGG QKQRLAIRRA LIRQPRVLLL 

      1210       1220       1230       1240       1250       1260 
DEATSALDTE SEKVVQEALD KAREGRTCIV IAHRLSTIQN ADLIVVIQNG KVKEHGTHQQ 

      1270       1280 
LLAQKGIYFS MVNIQAGAQN S

« Hide

References

[1]"Complete cDNA sequences encoding the Chinese hamster P-glycoprotein gene family."
Endicott J.A., Sarangi F., Ling V.
DNA Seq. 2:89-101(1991) [PubMed: 1685679] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M60042 mRNA. Translation: AAA68885.1.
PIRI48120.
I48123.
RefSeqNP_001230916.1. NM_001243987.1.

3D structure databases

ProteinModelPortalP23174.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100682535.

Phylogenomic databases

HOVERGENHBG080809.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF90123. ABC_TM_1. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDR3_CRIGR
AccessionPrimary (citable) accession number: P23174
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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