P23170 (25KD_SARPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Development-specific 25 kDa protein
Organism	Sarcophaga peregrina (Flesh fly) (Boettcherisca peregrina)
Taxonomic identifier	7386 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Oestroidea › Sarcophagidae › Sarcophaga › Boettcherisca

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Developmental stage	Expressed in the fat body of middle third-instar larvae.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Development-specific 25 kDa protein		PRO_0000054445
Regions
	Nucleotide binding	10 – 34	25	NAD or NADP By similarity
Sites
	Active site	151	1	Proton acceptor By similarity
	Binding site	138	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23170 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 651CB188A5D1547A	FASTA	258	28,633
	10 20 30 40 50 60 MMDWNNKNVV YVGGFSGFGY QVCQMMMKKP MKHLIVCSRM ENVEMLKKLQ AINTSVKVMF 70 80 90 100 110 120 VQMNIADYAS IVKGVKQVIG HVGHVDVLIN GVGGLADKDV ETTVAVNLTG LINTTLMFMP 130 140 150 160 170 180 YMDKTQSGHG GMVVSISSVY GLEPGPAFSV YSAAKHGGIG FTRSMADEHL YHKTGVAFMC 190 200 210 220 230 240 ICPAMTSTEL MMNKRDMNWM KWVPHSEEMW KMVMDAKMQT PEECAVNMMT AMEQAKNGAI 250 YICSTSGMKE ITPTVYMH

References

[1]	"Structural analysis of a developmentally regulated 25-kDa protein gene of Sarcophaga peregrina." Matsumoto N., Sekimizu K., Soma G., Ohmura Y., Andoh T., Nakanishi Y., Obinata M., Natori S. J. Biochem. 97:1501-1508(1985) [PubMed: 2993269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Purification and cDNA cloning of the alcohol dehydrogenase of the flesh fly Sarcophaga peregrina. A structural relationship between alcohol dehydrogenase and a 25-kDa protein." Horio T., Kubo T., Natori S. Eur. J. Biochem. 237:698-703(1996) [PubMed: 8647115] [Abstract] Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X02570 Genomic DNA. Translation: CAA26412.1.
PIR	A24181. T11741.
3D structure databases
ProteinModelPortal	P23170.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002426. ADH_Ceratitis-type. IPR002424. ADH_insect. IPR002198. DH_sc/Rdtase_SDR. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR01169. CERATITISADH. PR01167. INSADHFAMILY. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

25KD_SARPE

Accession

Primary (citable) accession number: P23170

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)