Reviewed, UniProtKB/Swiss-Prot P23160 (R34K_CLOPA)

Names and origin

Protein names	Recommended name:     34.2 kDa protein in rubredoxin operon     EC=1.6.4.- Alternative name(s):     ORF A
Organism	Clostridium pasteurianum
Taxonomic identifier	1501 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Sequence similarities

Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	34.2 kDa protein in rubredoxin operon		PRO_0000166790
Regions
	Nucleotide binding	268 – 278	11	FAD By similarity
Amino acid modifications
	Disulfide bond	136 ↔ 139		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23160-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 52D9CF51208EFC37	FASTA	308	34,215
	10 20 30 40 50 60 MKEEKQLDLV IIGAGPAGLT AAIYAIRAKL NTLVLENELV GGQIRETYTV ENFPGFNVIS 70 80 90 100 110 120 GADLADKMEE HAASIGVNID QFSNIEKIKL SDDEKIIETE DVIYKVKALI IATGAKSRRL 130 140 150 160 170 180 PIPEEEKLHG KVIHYCELCD GALYQGKDLV VVGGGNSAVE AAIFLTKYAR NITIVHQFDY 190 200 210 220 230 240 LQAQKYSQDE LFKHKNVKII WDSEIRNIVG ENEIEKIVVE NVKTKQKTEL KADGVFVYIG 250 260 270 280 290 300 YEPKTELFKD SININKWGYI ETDENMETNI KGVFAAGDVR SKLIRQLTTA VSDGTVAALM AEKYIGGK

References

[1]	"Cloning, sequencing and expression in Escherichia coli of the rubredoxin gene from Clostridium pasteurianum." Mathieu I., Meyer J., Moulis J.-M. Biochem. J. 285:255-262(1992) [PubMed: 1637309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60116 Genomic DNA. Translation: AAA23276.1.
PIR	S29117.
3D structure databases
HSSP	HSSP built from PDB template 1VDC based on UniProtKB Q39243.
ModBase	Search...
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. [Graphical view]
Pfam	PF00070. Pyr_redox. 2 hits. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

R34K_CLOPA

Accession

Primary (citable) accession number: P23160

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)