Reviewed, UniProtKB/Swiss-Prot P23147 (LUXB2_PHOLU)

Names and origin

Protein names

Recommended name:
    Alkanal monooxygenase beta chain
    EC=1.14.14.3
Alternative name(s):
    Bacterial luciferase beta chain

Gene names

Name:

luxB

Organism

Photorhabdus luminescens (Xenorhabdus luminescens)

Taxonomic identifier

29488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity.
Catalytic activity	RCHO + reduced FMN + O2 = RCOOH + FMN + H2O + light.
Subunit structure	Heterodimer of an alpha and a beta chain.
Sequence similarities	Belongs to the bacterial luciferase oxidoreductase family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	FMN Flavoprotein
Molecular function	Monooxygenase Oxidoreductase Photoprotein
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkanal monooxygenase (FMN-linked) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Alkanal monooxygenase beta chain		PRO_0000220178

Sequences

	Sequence		Length	Mass (Da)
	P23147-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: E7D81324D344A975	FASTA	324	37,115
	10 20 30 40 50 60 MKFGLFFLNF INSTTIQEQS IARMQEITEY VDKLNFEQIL VCENHFSDNG VVGAPLTVSG 70 80 90 100 110 120 FLLGLTEKIK IGSLNHVITT HHPVRIAEEA CLLDQLSEGR FILGFSDCER KDEMPFFNRP 130 140 150 160 170 180 EQYQQQLFEE CYDIINDALT TGYCNPNGDF YNFPKISMNP HAYTQNGPRK YVTATSCHVV 190 200 210 220 230 240 EWAAKKGIPL IFKWDDSNEV KHEYAKRYQA IAGEYGVDLA EIDHQLMILV NYSEDSEKAK 250 260 270 280 290 300 EETRAFISDY ILAMHPNENF EKKLEEIITE NSVGDYMECT TAAKLAMEKC GTKGILLSFE 310 320 SMSDFTHQIN AIDIVNDNIK KYHM

References

[1]	"Cloning and nucleotide sequences of lux genes and characterization of luciferase of Xenorhabdus luminescens from a human wound." Xi L., Cho K.W., Tu S.C. J. Bacteriol. 173:1399-1405(1991) [PubMed: 1995589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hw.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62917 Genomic DNA. Translation: AAA63566.1.
3D structure databases
HSSP	HSSP built from PDB template 1BSL based on UniProtKB P07739.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.14.3. 257143.
Family and domain databases
InterPro	IPR018235. Bacterial_luciferase_CS. IPR011251. Luciferase-like. IPR016048. Luciferase-like_sub. IPR002103. Luciferase_bac. [Graphical view]
Gene3D	G3DSA:3.20.20.30. Luciferase_like. 1 hit.
Pfam	PF00296. Bac_luciferase. 1 hit. [Graphical view]
PRINTS	PR00089. LUCIFERASE.
PROSITE	PS00494. BACTERIAL_LUCIFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXB2_PHOLU

Accession

Primary (citable) accession number: P23147

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)