Reviewed, UniProtKB/Swiss-Prot P23136 (UCRI_RHORU)

Names and origin

Protein names

Recommended name:
    Ubiquinol-cytochrome c reductase iron-sulfur subunit
    EC=1.10.2.2
Alternative name(s):
    Rieske iron-sulfur protein
      Short name=RISP

Gene names

Name:	petA
Synonyms:	fbcF

Organism

Rhodospirillum rubrum

Taxonomic identifier

1085 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length	183 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein.
Subcellular location	Cell inner membrane; Single-pass membrane protein.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 183	182	Ubiquinol-cytochrome c reductase iron-sulfur subunit		PRO_0000127763
Regions
	Transmembrane	21 – 41	21	Potential
	Domain	88 – 181	94	Rieske
Sites
	Metal binding	121	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	123	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	145	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	148	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	126 ↔ 147		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23136-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 915729F04701D7F9	FASTA	183	19,513
	10 20 30 40 50 60 MAEAEHTAST PGGESSRRDF LIYGTTAVGA VGVALAVWPF IDFMNPAADT LALASTEVDV 70 80 90 100 110 120 SAIAEGQAIT VTWRGKPVFV RHRTQKEIVV ARAVDPASLR DPQTDEARVQ QAQWLVMVGV 130 140 150 160 170 180 CTHLGCIPLG QKAGDPKGDF DGWFCPCHGS HYDSAGRIRK GPAPLNLPVP PYAFTDDTTV LIG

References

[1]	"The pet genes of Rhodospirillum rubrum: cloning and sequencing of the genes for the cytochrome bc1-complex." Majewski C., Trebst A. Mol. Gen. Genet. 224:373-382(1990) [PubMed: 2176269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-35. Strain: FR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55387 Genomic DNA. Translation: CAA39058.1.
PIR	RDQFBR. S12256.
3D structure databases
SMR	P23136. Positions 17-183.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.10.2.2. 1671.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. IPR019470. Ubiq_cytC_Rdtase_Fe-S_su_TAT. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF10399. UCR_Fe-S_N. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_RHORU

Accession

Primary (citable) accession number: P23136

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)