P23134 (CYB_RHORU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome b

Gene names

Name:

petB

Organism

Rhodospirillum rubrum

Taxonomic identifier

1085 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodospirillales › Rhodospirillaceae › Rhodospirillum

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis By similarity.
Cofactor	Binds 2 heme groups non-covalently By similarity.
Subunit structure	The main subunits of complex b-c1 are: cytochrome b, cytochrome c1 and the Rieske protein By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Miscellaneous	Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm By similarity.
Sequence similarities	Belongs to the cytochrome b family.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	respiratory electron transport chain Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Cytochrome b		PRO_0000061773
Regions
	Transmembrane	44 – 64	21	Helical; Potential
	Transmembrane	97 – 117	21	Helical; Potential
	Transmembrane	124 – 144	21	Helical; Potential
	Transmembrane	163 – 183	21	Helical; Potential
	Transmembrane	191 – 211	21	Helical; Potential
	Transmembrane	245 – 265	21	Helical; Potential
	Transmembrane	303 – 323	21	Helical; Potential
	Transmembrane	338 – 358	21	Helical; Potential
	Transmembrane	368 – 388	21	Helical; Potential
Sites
	Metal binding	94	1	Iron 1 (heme b562 axial ligand)
	Metal binding	108	1	Iron 2 (heme b566 axial ligand)
	Metal binding	195	1	Iron 1 (heme b562 axial ligand)
	Metal binding	209	1	Iron 2 (heme b566 axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	P23134 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: F19452FDA318BE6F	FASTA	405	46,369
	10 20 30 40 50 60 MYTPPRWNNK ALKWFDERLP VLTVAHKELV VYPAPRNLNY FWNFGSLAGI AMIIMIATGI 70 80 90 100 110 120 FLAMSYTAHV DHAFDSVERI MRDVNYGWLM RYMHANGASM FFIVVYVHMF RGLYYGSYKP 130 140 150 160 170 180 PREVLWWLGL VILLLMMATA FMGYVLPWGQ MSFWGATVIT NLFSAIPVVG DDIVTLLWGG 190 200 210 220 230 240 FSVDNPTLNR FFSLHYLFPM LLFAVVFLHM WALHVKKSNN PLGIDAKGPF DTIPFHPYYT 250 260 270 280 290 300 VKDAFGLGIF LMVFCFFVFF APNFFGEPDN YIPANPMVTP THIVPEWYFL PFYAILRAVP 310 320 330 340 350 360 DKLGGVLAMF GAILILFVLP WLDTSKVRSA TFRPVFKGFF WVFLADCLLL GYLGAMPAEE 370 380 390 400 PYVTITQLAT IYYFLHFLVI TPLVGWFEKP KPLPVSISSP VTTQA

References

[1]	"The pet genes of Rhodospirillum rubrum: cloning and sequencing of the genes for the cytochrome bc1-complex." Majewski C., Trebst A. Mol. Gen. Genet. 224:373-382(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-27. Strain: FR1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X55387 Genomic DNA. Translation: CAA39059.1.
PIR	CBQFR. S12257.
3D structure databases
ProteinModelPortal	P23134.
SMR	P23134. Positions 17-390.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016175. Cyt_b/b6. IPR005798. Cyt_b/b6_C. IPR005797. Cyt_b/b6_N. IPR016174. Di-haem_cyt_TM. [Graphical view]
PANTHER	PTHR19271. PTHR19271. 1 hit.
Pfam	PF00032. Cytochrom_B_C. 1 hit. PF13631. Cytochrom_B_N_2. 1 hit. [Graphical view]
SUPFAM	SSF81648. Cytochrome_b/b6_C. 1 hit. SSF81342. Transmembr_di-haem_cytochrome. 1 hit.
PROSITE	PS51003. CYTB_CTER. 1 hit. PS51002. CYTB_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB_RHORU

Accession

Primary (citable) accession number: P23134

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program