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P23129 (ODO1_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-oxoglutarate dehydrogenase E1 component

EC=1.2.4.2
Alternative name(s):
Alpha-ketoglutarate dehydrogenase
Gene names
Name:odhA
Synonyms:citK
Ordered Locus Names:BSU19370
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length944 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). HAMAP-Rule MF_01169

Catalytic activity

2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2. HAMAP-Rule MF_01169

Cofactor

Thiamine pyrophosphate.

Subunit structure

Homodimer.

Sequence similarities

Belongs to the alpha-ketoglutarate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9449442-oxoglutarate dehydrogenase E1 component HAMAP-Rule MF_01169
PRO_0000162169

Experimental info

Sequence conflict351N → Y in CAA38576. Ref.1
Sequence conflict1101L → S in AAC17864. Ref.5
Sequence conflict1331P → L in AAC17864. Ref.5
Sequence conflict2311I → T in AAC17864. Ref.5
Sequence conflict275 – 2773NKD → RS in CAA38576. Ref.1
Sequence conflict283 – 31634GSIGI…SARIT → DPLESATDGRGMSNTIWGRI GSFKTLKQNQPALP in CAA38576. Ref.1
Sequence conflict488 – 4947VKQIFAE → SNKFSLK in CAA38576. Ref.1
Sequence conflict520 – 5223DAY → VAI in CAA38576. Ref.1
Sequence conflict554 – 5563DFD → HFH in CAA38576. Ref.1
Sequence conflict567 – 5693NWP → TG in CAA38576. Ref.1
Sequence conflict573 – 58513NVFGK…ILERR → MFSQAKAHFRKT in CAA38576. Ref.1
Sequence conflict837 – 8382SD → DV in AAA22628. Ref.6
Sequence conflict8381D → V in CAA38576. Ref.1
Sequence conflict927 – 94418VHKKE…LTRKN → EFIKKNRNVLYLIA in CAA38576. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P23129 [UniParc].

Last modified July 28, 2009. Version 3.
Checksum: DCBC7B28C44A9CC2

FASTA944106,278
        10         20         30         40         50         60 
MFQNSMKQRM NWEDFYGPNL GYALELYDQY TQDPNSIDPD LKEMFDELGA PPSDIKEASG 

        70         80         90        100        110        120 
TKEKGRVTAD LIQKIASAVR LAEDIRTYGH LNASVNPLRK DEKKSELFPL SDYGLTEEEI 

       130        140        150        160        170        180 
KAIPASVICK DAPKNISNGL EAIQYLRNTY KRTISFEFDH VHDFKEREWL TRKIESGELF 

       190        200        210        220        230        240 
QKNSAEKLSA VLERLTEVEG FEQFLHRTFV GQKRFSIEGL DALVPVLDDI IAQSVKSGTT 

       250        260        270        280        290        300 
SVNIGMAHRG RLNVLAHVLG KPYEIIFSEF QHAPNKDLVP SEGSIGISYG WTGDVKYHLG 

       310        320        330        340        350        360 
ANRELQDAET KSARITLANN PSHLEFINPI VEGSTRAAQE TRTQSGYPVQ DETKSLAILI 

       370        380        390        400        410        420 
HGDAAFPGEG IVAETLNLSS LKGYQVGGAI HIIANNMIGF TTESAESRST KYASDLAKGY 

       430        440        450        460        470        480 
EIPIVHVNAD DPEACLSAVK FAVEYRKTFN KDFLIDLIGY RRYGHNEMDE PSTTQPMLYD 

       490        500        510        520        530        540 
AVRKHPTVKQ IFAEKLVKEG VVTEEVVQNI EKSVTKRIED AYQKVPSKKE HTACEIELPE 

       550        560        570        580        590        600 
PVSNGFPDVD TSIDFDVLRK LNGELINWPE SFNVFGKLKR ILERRAKAFD DDRKVEWSLA 

       610        620        630        640        650        660 
ESLAFASILK DGTPIRLTGQ DSERGTFAQR NLVLHDSETG KEFVPLHHLS DCSTSFAVHN 

       670        680        690        700        710        720 
SPLSEGSVLG FEYGYNVHSP ETLVLWEAQY GDFANAAQVY FDQFISAGRA KWGQKSGLVM 

       730        740        750        760        770        780 
LLPHGYEGQG PEHSSGRIER FLQLAAENNW TVANLTSAAQ YFHILRRQAK MLLREEIRPL 

       790        800        810        820        830        840 
VIMTPKSLLR NPNTVSEVQE LSESRFQPVY EQSGLSHDYE KVTRLVLSSG KVSIDISDHF 

       850        860        870        880        890        900 
NKLEDGKEWL HIARIEQLYP FPAKGVKELF AKLPNLKEIV WVQEEPQNMG AWGYISPYLT 

       910        920        930        940 
EIAPEGVSVQ YIGRRRRSSP AEGDPTVHKK EQERIVSDSL TRKN 

« Hide

References

« Hide 'large scale' references
[1]"Organization and regulation of the Bacillus subtilis odhAB operon, which encodes two of the subenzymes of the 2-oxoglutarate dehydrogenase complex."
Resnekov O., Melin L., Carlsson P., Mannerloev M., von Gabain A., Hederstedt L.
Mol. Gen. Genet. 234:285-296(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Detecting and analyzing DNA sequencing errors: toward a higher quality of the Bacillus subtilis genome sequence."
Medigue C., Rose M., Viari A., Danchin A.
Genome Res. 9:1116-1127(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[4]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[5]"Sequence analysis of the Bacillus subtilis 168 chromosome region between the sspC and odhA loci (184 degrees-180 degrees)."
Ghim S.-Y., Choi S.-K., Shin B.-S., Jeong Y.-M., Sorokin A., Ehrlich S.D., Park S.-H.
DNA Res. 5:195-201(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-270.
Strain: 168.
[6]"Genetic characterization of Bacillus subtilis odhA and odhB, encoding 2-oxoglutarate dehydrogenase and dihydrolipoamide transsuccinylase, respectively."
Carlsson P., Hederstedt L.
J. Bacteriol. 171:3667-3672(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 652-944.
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54805 Genomic DNA. Translation: CAA38576.1.
AL009126 Genomic DNA. Translation: CAB13829.3.
AF026147 Genomic DNA. Translation: AAC17864.1.
M27141 Genomic DNA. Translation: AAA22628.1.
PIRA32879. S25295.
RefSeqNP_389819.3. NC_000964.3.

3D structure databases

ProteinModelPortalP23129.
SMRP23129. Positions 71-943.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU19370.

Proteomic databases

PaxDbP23129.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB13829; CAB13829; BSU19370.
GeneID939507.
KEGGbsu:BSU19370.
PATRIC18975725. VBIBacSub10457_2053.

Organism-specific databases

GenoListBSU19370. [Micado]

Phylogenomic databases

eggNOGCOG0567.
HOGENOMHOG000259588.
KOK00164.
OrthoDBEOG6V1M1F.
PhylomeDBP23129.

Enzyme and pathway databases

BioCycBSUB:BSU19370-MONOMER.

Family and domain databases

Gene3D3.40.50.970. 2 hits.
HAMAPMF_01169. SucA_OdhA.
InterProIPR011603. 2oxoglutarate_DH_E1.
IPR023784. 2oxoglutarate_DH_E1_bac.
IPR001017. DH_E1.
IPR029061. THDP-binding.
IPR005475. Transketolase-like_Pyr-bd.
[Graphical view]
PANTHERPTHR23152. PTHR23152. 1 hit.
PfamPF00676. E1_dh. 1 hit.
PF02779. Transket_pyr. 1 hit.
[Graphical view]
PIRSFPIRSF000157. Oxoglu_dh_E1. 1 hit.
SMARTSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMSSF52518. SSF52518. 2 hits.
TIGRFAMsTIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNetSearch...

Entry information

Entry nameODO1_BACSU
AccessionPrimary (citable) accession number: P23129
Secondary accession number(s): O68261, Q45642
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: July 28, 2009
Last modified: July 9, 2014
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList