Reviewed, UniProtKB/Swiss-Prot P23128 (DDX6_DROME)

Names and origin

Protein names

Recommended name:
    Putative ATP-dependent RNA helicase me31b
    EC=3.6.1.-
Alternative name(s):
    Maternal expression at 31B

Gene names

Name:	me31B
ORF Names:	CG4916

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Unwinds RNA in an ATP-dependent fashion Potential.
Developmental stage	Expressed both maternally and zygotically. Ref.1
Sequence similarities	Belongs to the DEAD box helicase family. DDX6/DHH1 subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent RNA helicase activity Non-traceable author statement. Source: FlyBase RNA binding Inferred from direct assay. Source: FlyBase protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q9W1V7	1	EBI-300281,EBI-168552
cup	Q9VMA3	1	EBI-300281,EBI-95398
mei-P26	Q9W378	1	EBI-300281,EBI-187428

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P23128-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P23128-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Putative ATP-dependent RNA helicase me31b		PRO_0000054982
Regions
	Domain	89 – 259	171	Helicase ATP-binding
	Domain	269 – 429	161	Helicase C-terminal
	Nucleotide binding	102 – 109	8	ATP By similarity
	Motif	58 – 86	29	Q motif
	Motif	207 – 210	4	DEAD box
Amino acid modifications
	Modified residue	8	1	Phosphoserine Ref.5
	Modified residue	29	1	Phosphoserine Ref.5
	Modified residue	450	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	1 – 31	31	Missing in isoform B.		VSP_019286
Experimental info
	Sequence conflict	39 – 45	7	KLPPKDN → NCRQRTT in AAA28603. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified February 2, 2004. Version 3. Checksum: B783E8185EF11E86	FASTA	459	51,945
	10 20 30 40 50 60 MMTEKLNSGH TNLTSKGIIN DLQIAGNTSD DMGWKSKLKL PPKDNRFKTT DVTDTRGNEF 70 80 90 100 110 120 EEFCLKRELL MGIFEKGWER PSPIQEAAIP IALSGKDVLA RAKNGTGKTG AYCIPVLEQI 130 140 150 160 170 180 DPTKDYIQAL VMVPTRELAL QTSQICIELA KHLDIRVMVT TGGTILKDDI LRIYQKVQLI 190 200 210 220 230 240 IATPGRILDL MDKKVADMSH CRILVLDEAD KLLSLDFQGM LDHVILKLPK DPQILLFSAT 250 260 270 280 290 300 FPLTVKNFME KHLREPYEIN LMEELTLKGV TQYYAFVQER QKVHCLNTLF SKLQINQSII 310 320 330 340 350 360 FCNSTQRVEL LAKKITELGY CCYYIHAKMA QAHRNRVFHD FRQGLCRNLV CSDLFTRGID 370 380 390 400 410 420 VQAVNVVINF DFPRMAETYL HRIGRSGRFG HLGIAINLIT YEDRFDLHRI EKELGTEIKP 430 440 450 IPKVIDPALY VANVGASVGD TCNNSDLNNS ANEEGNVSK
	Isoform B. Checksum: 34E61B3C97D11A2A	FASTA	428	48,644
	10 20 30 40 50 60 MGWKSKLKLP PKDNRFKTTD VTDTRGNEFE EFCLKRELLM GIFEKGWERP SPIQEAAIPI 70 80 90 100 110 120 ALSGKDVLAR AKNGTGKTGA YCIPVLEQID PTKDYIQALV MVPTRELALQ TSQICIELAK 130 140 150 160 170 180 HLDIRVMVTT GGTILKDDIL RIYQKVQLII ATPGRILDLM DKKVADMSHC RILVLDEADK 190 200 210 220 230 240 LLSLDFQGML DHVILKLPKD PQILLFSATF PLTVKNFMEK HLREPYEINL MEELTLKGVT 250 260 270 280 290 300 QYYAFVQERQ KVHCLNTLFS KLQINQSIIF CNSTQRVELL AKKITELGYC CYYIHAKMAQ 310 320 330 340 350 360 AHRNRVFHDF RQGLCRNLVC SDLFTRGIDV QAVNVVINFD FPRMAETYLH RIGRSGRFGH 370 380 390 400 410 420 LGIAINLITY EDRFDLHRIE KELGTEIKPI PKVIDPALYV ANVGASVGDT CNNSDLNNSA NEEGNVSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second maternally expressed Drosophila gene encodes a putative RNA helicase of the 'DEAD box' family." de Valoir T., Tucker M.A., Belikoff E.J., Camp L.A., Bolduc C., Beckingham K. Proc. Natl. Acad. Sci. U.S.A. 88:2113-2117(1991) [PubMed: 1900936] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), DEVELOPMENTAL STAGE.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A). Strain: Berkeley. Tissue: Embryo.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8; SER-29 AND SER-450, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M59926 mRNA. Translation: AAA28603.1. AE014134 Genomic DNA. Translation: AAF52881.2. AE014134 Genomic DNA. Translation: AAN10728.1. AY051663 mRNA. Translation: AAK93087.1.
PIR	A39157.
RefSeq	NP_523533.2. NP_723539.1.
UniGene	Dm.2770
3D structure databases
HSSP	HSSP built from PDB template 1QDE based on UniProtKB P10081.
SMR	P23128. Positions 58-432.
ModBase	Search...
Protein-protein interaction databases
IntAct	P23128. 16 interactions.
Genome annotation databases
Ensembl	FBgn0004419. Drosophila melanogaster. [Contig view]
GeneID	34364.
KEGG	dme:Dmel_CG4916.
Organism-specific databases
FlyBase	FBgn0004419. me31B.
Phylogenomic databases
HOGENOM	P23128.
OMA	P23128. YKIEQEL.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-008302-MON.
Gene expression databases
ArrayExpress	P23128.
GermOnline	CG4916. Drosophila melanogaster.
Family and domain databases
InterPro	IPR014001. DEAD-like_N. IPR001650. DNA/RNA_helicase_C. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014021. Helicase_SF1/SF2_ATP-bd. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	788139.

Entry information

Entry name

DDX6_DROME

Accession

Primary (citable) accession number: P23128
Secondary accession number(s): Q8IPC9, Q961D2, Q9VL17

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	February 2, 2004
Last modified:	June 16, 2009
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project