Reviewed, UniProtKB/Swiss-Prot P23113 (LUXC_PHOLU)

Names and origin

Protein names

Recommended name:
    Acyl-CoA reductase
    EC=1.2.1.50

Gene names

Name:

luxC

Organism

Photorhabdus luminescens (Xenorhabdus luminescens)

Taxonomic identifier

29488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Photorhabdus

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	LuxC is the fatty acid reductase enzyme responsible for synthesis of the aldehyde substrate for the luminescent reaction catalyzed by luciferase.
Catalytic activity	A long-chain aldehyde + CoA + NADP+ = a long-chain acyl-CoA + NADPH.
Pathway	Lipid metabolism; bioluminescent fatty acid reduction.
Sequence similarities	Belongs to the luxC family.

Ontologies

Keywords
Biological process	Luminescence
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	acyl-CoA reductase activity Inferred from electronic annotation. Source: InterPro long-chain-fatty-acyl-CoA reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 480	480	Acyl-CoA reductase		PRO_0000220199
Experimental info
	Sequence conflict	2 – 3	2	NK → PP in AAA63563. Ref.1
	Sequence conflict	81	1	T → P in AAA63563. Ref.1
	Sequence conflict	86 – 95	10	LKRYMGYSEE → PKKIYGIFRR in AAA63563. Ref.1
	Sequence conflict	103	1	W → R in AAA63563. Ref.1
	Sequence conflict	196	1	D → V in AAA63563. Ref.1
	Sequence conflict	209	1	Y → D in AAA63563. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P23113-1 [UniParc]. Last modified May 10, 2004. Version 2. Checksum: 57C40EF27BB3810D	FASTA	480	54,847
	10 20 30 40 50 60 MNKKISFIIN GRVEIFPESD DLVQSINFGD NSVHLPVLND SQVKNIIDYN ENNELQLHNI 70 80 90 100 110 120 INFLYTVGQR WKNEEYSRRR TYIRDLKRYM GYSEEMAKLE ANWISMILCS KGGLYDLVKN 130 140 150 160 170 180 ELGSRHIMDE WLPQDESYIR AFPKGKSVHL LTGNVPLSGV LSILRAILTK NQCIIKTSST 190 200 210 220 230 240 DPFTANALAL SFIDVDPHHP VTRSLSVVYW QHQGDISLAK EIMQHADVVV AWGGEDAINW 250 260 270 280 290 300 AVKHAPPDID VMKFGPKKSF CIIDNPVDLV SAATGAAHDV CFYDQQACFS TQNIYYMGSH 310 320 330 340 350 360 YEEFKLALIE KLNLYAHILP NTKKDFDEKA AYSLVQKECL FAGLKVEVDV HQRWMVIESN 370 380 390 400 410 420 AGVELNQPLG RCVYLHHVDN IEQILPYVRK NKTQTISVFP WEAALKYRDL LALKGAERIV 430 440 450 460 470 480 EAGMNNIFRV GGAHDGMRPL QRLVTYISHE RPSHYTAKDV AVEIEQTRFL EEDKFLVFVP

References

[1]	"Cloning and nucleotide sequences of lux genes and characterization of luciferase of Xenorhabdus luminescens from a human wound." Xi L., Cho K.W., Tu S.C. J. Bacteriol. 173:1399-1405(1991) [PubMed: 1995589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hw.
[2]	"Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria." Meighen E.A., Szittner R.B. J. Bacteriol. 174:5371-5381(1992) [PubMed: 1644764] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Hw.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62917 Genomic DNA. Translation: AAA63563.1. M90092 Genomic DNA. Translation: AAD05355.1.
PIR	A42951.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.50. 257143.
Family and domain databases
InterPro	IPR008670. LuxC. [Graphical view]
Pfam	PF05893. LuxC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LUXC_PHOLU

Accession

Primary (citable) accession number: P23113
Secondary accession number(s): Q56820

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	May 10, 2004
Last modified:	June 16, 2009
This is version 42 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)